Pharmaceutical composition for treating oral cancer as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a pharmaceutical composition for treating oral cancer and a preparation method and application thereof. The pharmaceutical composition for treating oral cancer provided by the invention is prepared from the following raw materials in parts by weight: 10 to 40 parts of American cockroach, 10 to 30 parts of solidago decurrens, 10 to 20 parts of radix rehmanniae, 10 to 30 parts of picria fel-terrae, 8 to 15 parts of bunge corydalis herb, 10 to 20 parts of semen coicis and 0.5 to 2 parts of luteolin. The pharmaceutical composition can inhibit proliferation of oral cancer cells, induce apoptosis of the oral cancer cells and inhibit migration activity of the oral cancer cells, and has a good treatment effect on oral cancer.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine compositions, and in particular relates to a pharmaceutical composition for treating oral cancer, a preparation method and use thereof. Background Art

[0002] Oral cancer is one of the most common malignant tumors in the head and neck, accounting for about 3% of all malignant cancers in the body. It has a high incidence rate and seriously endangers human health. In recent years, with the rapid development of the global economy and the improvement of people's living standards, the incidence of oral cancer has increased significantly, and the incidence population has tended to be younger.

[0003] At present, the treatment of oral cancer is mainly based on surgery, supplemented by radiotherapy and chemotherapy, but these can cause defects in oral and maxillofacial tissues and organs, leading to functional disorders such as speech, chewing, and swallowing in patients, which seriously affects the production quality of patients. In addition, chemotherapy is also an important treatment for oral cancer. The therapeutic drugs include fluorouracil and cisplatin, but the existing chemotherapy methods not only have great toxic side effects, but also easily produce drug resistance. Therefore, finding highly effective and low-toxic drugs for the prevention and treatment of oral cancer remains a top priority for scientific researchers.

[0004] Periplaneta americana is an insect belonging to the genus Periplaneta in the order Blattaria of the family Blattidae, class Insecta. It is commonly known as "cockroach". Its use as medicine was first recorded in Shennong's Herbal Classic and has been recorded in important herbal classics of all dynasties. It can treat blood stasis, cold and heat, accumulation, throat and pharynx numbness, internal cold and infertility; it can be used for scar accumulation, pediatric carbuncle, beriberi, sore swelling, and mosquito bites. In recent years, the research and development of traditional Chinese medicine resources has received increasing attention, and more and more research has been conducted on the medicinal value of Periplaneta americana. At present, there is no report on the use of a pharmaceutical composition containing Periplaneta americana for the treatment of oral cancer. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a pharmaceutical composition for treating oral cancer.

[0006] The pharmaceutical composition provided by the present invention comprises the following raw materials in parts by weight: 10 to 40 parts by weight of American cockroach, 10 to 30 parts by weight of Solidago asiatica, 10 to 20 parts by weight of Radix Rehmanniae, 10 to 30 parts by weight of Scrophularia ningpoensis, 8 to 15 parts by weight of Herba Coicis, 10 to 20 parts by weight of Semen Coicis and 0.5 to 2 parts by weight of Luteolin.

[0007] Furthermore, the pharmaceutical composition comprises the following raw materials in parts by weight: 15 to 35 parts by weight of American cockroach, 10 to 20 parts by weight of Solidago asiatica, 15 to 20 parts by weight of Radix Rehmanniae, 10 to 25 parts by weight of Scrophularia ningpoensis, 9 to 13 parts by weight of Herba Coicis, 10 to 15 parts by weight of Semen Coicis, and 0.6 to 1.2 parts by weight of luteolin.

[0008] The American cockroach (Periplaneta americana) is an insect of the genus Periplaneta in the order Blattaria, family Blattidae, class Insecta. Its medicinal use was first seen in Shennong's Herbal Classic, where it was listed as a medium-grade product, which is described as "salty and flat". It is used to treat blood phlegm, cold and heat, breaking up accumulation, throat and pharyngeal paralysis, and internal cold and infertility; it has the effects of promoting blood circulation and removing blood stasis, detoxifying and eliminating malnutrition, promoting diuresis and reducing swelling, and contains a variety of effective ingredients such as protein, amino acids, peptides, sugars, and polyols. Modern research shows that the American cockroach extract has significant effects in anti-tumor, improving immunity, treating cardiovascular diseases, tissue repair, antibacterial, and anti-inflammatory.

[0009] Solidago decurrens Lour. is the dried whole herb of Solidago decurrens Lour. of the Asteraceae family. It tastes pungent and bitter, and is cool in nature. It enters the lung and liver meridians. It clears away heat and detoxifies, and dispels wind-heat. It is used for laryngeal paralysis, pharyngitis, sore throat, sore and swollen toxins, and wind-heat colds.

[0010] Rehmannia glutinosa is the fresh or dried root of Rehmannia glutinosa Libosch., a plant of the Scrophulariaceae family. Rehmannia glutinosa is slowly roasted until it is about 80% dry and is commonly known as "raw rehmannia". Raw rehmannia is sweet in taste and cold in nature. It enters the heart, liver and kidney meridians. Raw rehmannia clears heat and cools blood, nourishes yin and produces body fluid. It is used for heat entering the blood, fever caused by warm toxins, vomiting blood and epistaxis, damage to yin due to febrile diseases, red tongue and thirst, constipation caused by damage to body fluid, fever due to yin deficiency, bone steaming and fatigue fever, and internal heat and thirst.

[0011] Scrophulariaceae is the dried whole herb of Scrophulariaceae. It is bitter and cold in nature. It enters the lung, stomach and liver meridians. It clears away heat and detoxifies, and relieves swelling and pain. It is used for wind-heat cold, sore throat, laryngeal paralysis, mumps, abdominal pain, dysentery, traumatic injury, carbuncle, and snake bites.

[0012] Corydalis bungeana Turcz. is the dried whole herb of Corydalis bungeana Turcz., a plant of the Poppy family. It tastes bitter and is cold in nature. It enters the heart, liver, and large intestine meridians. It clears away heat and detoxifies, dissipates stagnation and reduces swelling. It is used for epidemic colds, sore throats, sore furuncles, carbuncle on the back, and mumps and erysipelas.

[0013] Coix seeds are the dried mature seeds of Coixlacryma-jobi L. var. ma-yuen (Roman.) Stapf. It is sweet and light in taste and cool in nature. It enters the spleen, stomach and lung meridians. It can promote diuresis and eliminate dampness, strengthen the spleen and stop diarrhea, remove numbness, discharge pus, detoxify and disperse stagnation. It is used for edema, beriberi, dysuria, spleen deficiency diarrhea, dampness numbness and cramps, lung abscess, intestinal abscess, warts and cancer.

[0014] Luteolin (LUT) is a natural flavonoid compound. It was originally isolated from the leaves, stems and branches of the herbaceous plant Resedaodorata L. of the family Resedaceae. It can be isolated from a variety of natural medicinal materials, vegetables and fruits. It is currently found to be mainly present in natural Chinese medicines such as honeysuckle, schizonepeta, artichoke, perilla, scutellaria and vegetables such as cabbage, cauliflower and carrot. Studies have shown that it has multiple pharmacological effects such as anti-inflammatory, anti-allergic, anti-tumor, antioxidant, and protection of the nervous system. It is mainly used in the treatment of respiratory inflammatory diseases, tumor diseases and cardiovascular diseases.

[0015] The inventors have confirmed through a large number of experiments that the pharmaceutical composition prepared by compounding American cockroaches with Solidago asiatica, Rehmannia glutinosa, Scrophularia ningpoensis, Scutellaria baicalensis, Coix lachryma-jobi, and luteolin can inhibit the proliferation of oral cancer cells, induce apoptosis of oral cancer cells, and inhibit the migration activity of oral cancer cells, and has a good therapeutic effect on oral cancer.

[0016] The present invention also provides a method for preparing the pharmaceutical composition for treating oral cancer, comprising the following steps:

[0017] a. Crush the dried American cockroaches, soak them in an ethanol solution, reflux extract, concentrate until there is no alcohol taste, add water, mix well, filter, and obtain extract 1;

[0018] b. Mix and grind the solidago asiatica, radix rehmanniae, rhizoma sophorae flavescentis, rhizoma scrophulariae, rhizoma scrophulariae and coix seeds in the prescription, add water and boil, combine the decoctions, and filter to obtain extract 2;

[0019] c. Combine the extract 1 and the extract 2, concentrate under reduced pressure to obtain an extract, dry, and grind into extract powder, add luteolin, and mix to obtain the extract.

[0020] Furthermore, in step a, the concentration of the ethanol solution is 75% to 90%.

[0021] The soaking time is 10 to 12 hours.

[0022] Reflux extraction 2 to 3 times; the reflux extraction temperature is 70 to 80°C.

[0023] Concentrate until there is no alcohol taste, and add 5 to 8 times the amount of water as the raw material.

[0024] Furthermore, in step b, 9 to 12 times the total mass of water of Solidago asiatica, Radix Rehmanniae, Radix Sophorae Tonkinensis, Radix Scrophulariae, Herba Corydalis and Semen Coicis is added and boiled for 2 to 3 times.

[0025] The present invention also provides application of the pharmaceutical composition in preparing a medicine for treating oral cancer.

[0026] Furthermore, the pharmaceutical composition is used to inhibit the migration of oral cancer cells.

[0027] Furthermore, the pharmaceutical composition is used to induce apoptosis of oral cancer cells.

[0028] Furthermore, the pharmaceutical composition is used to inhibit the proliferation of oral cancer cells.

[0029] Furthermore, the drug for treating oral cancer consists of the pharmaceutical composition and a pharmaceutically acceptable carrier.

[0030] The beneficial effects of the present invention are:

[0031] The present invention prepares a prescription of American cockroach, Solidago asiatica, Radix Rehmanniae, Radix Scrophulariae, Radix Achyranthis Bidentatae, Semen Coix and luteolin, and coordinates the above raw materials to maximize the efficacy, enhance the synergistic effect between the medicinal materials, and make the prepared pharmaceutical composition have a good therapeutic effect on oral cancer. The pharmaceutical composition of the present invention can inhibit the proliferation of oral cancer cells and induce apoptosis of oral cancer cells on the one hand, and inhibit the migration activity of oral cancer cells on the other hand. The pharmaceutical composition of the present invention has no toxic side effects and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 is the apoptosis rate of human oral cancer KB cells in each group.

[0033] Figure 2 It is the proliferation inhibition rate of Example 1 group on human oral cancer KB cells.

[0034] Figure 3 It is the proliferation inhibition rate of Example 2 group on human oral cancer KB cells.

[0035] Figure 4 It is the proliferation inhibition rate of Example 3 group on human oral cancer KB cells.

[0036] Figure 5 This is the proliferation inhibition rate of the comparative example 1 group on human oral cancer KB cells.

[0037] Figure 6 The activity of each group on the migration of human oral cancer KB cells. DETAILED DESCRIPTION

[0038] The present invention will be further described in detail below through specific examples. The raw materials and reagents involved in the specific implementation method are all commercially available.

[0039] Example 1

[0040] Raw materials: 15 parts of American cockroach, 10 parts of Solidago asiatica, 15 parts of Radix Rehmanniae, 10 parts of Scrophulariae Radix, 9 parts of Scrophulariae Radix, 10 parts of Coix seeds, and 0.75 parts of Luteolin.

[0041] Preparation: a. Crush the dried American cockroach, soak it in 5 times the concentration of 75% ethanol solution for 10 hours, extract it under reflux at 75°C for 3 hours, add 2 times the concentration of 75% ethanol solution to extract it for the second time, extract it under reflux at 70°C for 2 hours, concentrate it until there is no alcohol taste, add 6 times the concentration of deionized water of the dried American cockroach, mix well, filter and obtain extract 1;

[0042] b. Mix and grind Solidago asiatica, Radix Rehmanniae, Radix Scrophulariae, Herba Coicis and Semen Coix, add 10 times the total weight of Solidago asiatica, Radix Rehmanniae, Radix Scrophulariae, Herba Coicis and Semen Coix, and boil twice, combine the decoctions, and filter to obtain Extract 2;

[0043] c. Combine the extract 1 and the extract 2, concentrate under reduced pressure to obtain an extract, dry, and grind into extract powder, add luteolin, and mix to obtain the extract.

[0044] Example 2

[0045] Raw materials: 30 parts of American cockroaches, 25 parts of Solidago asiatica, 18 parts of Radix Rehmanniae, 10 parts of Scrophulariae Radix, 13 parts of Scutellariae Herba, 15 parts of Coix seeds, and 1.5 parts of Luteolin.

[0046] Preparation: a. Crush the dried American cockroach, soak it in 5 times the concentration of 75% ethanol solution for 12 hours, extract it under reflux at 70°C for 3 hours, add 2 times the concentration of 75% ethanol solution to extract it for the second time, extract it under reflux at 75°C for 2 hours, concentrate it until there is no alcohol taste, add 7 times the concentration of deionized water of the dried American cockroach, mix well, filter and obtain extract 1;

[0047] b. Mix and grind Solidago asiatica, Radix Rehmanniae, Radix Scrophulariae, Herba Coicis and Semen Coix, add 11 times of the total weight of Solidago asiatica, Radix Rehmanniae, Radix Scrophulariae, Herba Coicis and Semen Coix, and boil twice, combine the decoctions, and filter to obtain Extract 2;

[0048] c. Combine the extract 1 and the extract 2, concentrate under reduced pressure to obtain an extract, dry, and grind into extract powder, add luteolin, and mix to obtain the extract.

[0049] Example 3

[0050] Raw materials: 35 parts of American cockroach, 20 parts of Solidago, 20 parts of Radix Rehmanniae, 25 parts of Scrophulariae Radix, 13 parts of Scutellariae Herba, 15 parts of Coix seeds, and 1.2 parts of Luteolin.

[0051] Preparation: a. Crush the dried American cockroach, soak it in 5 times the concentration of 80% ethanol solution for 10 hours, extract it under reflux at 70°C for 3 hours, add 2 times the concentration of 75% ethanol solution to extract it for the second time, extract it under reflux at 75°C for 2 hours, concentrate it until there is no alcohol taste, add 7 times the concentration of deionized water of the dried American cockroach, mix well, filter and obtain extract 1;

[0052] b. Mix and grind Solidago asiatica, Radix Rehmanniae, Radix Scrophulariae, Herba Coicis and Semen Coix, add 10 times the total weight of Solidago asiatica, Radix Rehmanniae, Radix Scrophulariae, Herba Coicis and Semen Coix, and boil twice, combine the decoctions, and filter to obtain Extract 2;

[0053] c. Combine the extract 1 and the extract 2, concentrate under reduced pressure to obtain an extract, dry, and grind into extract powder, add luteolin, and mix to obtain the extract.

[0054] Comparative Example 1

[0055] Raw materials: 10 parts of Solidago asiatica, 15 parts of Radix Rehmanniae, 10 parts of Scrophularia ningpoensis, 9 parts of Herba Coix, 10 parts of Semen Coicis, and 0.75 parts of Luteolin.

[0056] Mix and grind Solidago striata, Radix Rehmanniae, Scrophularia ningpoensis, Herba Coicis and Semen Coix, add 10 times of water in the total weight of Solidago striata, Radix Rehmanniae, Scrophularia ningpoensis, Herba Coicis and Semen Coix, and boil twice, combine the decoctions, filter to obtain the extract, concentrate under reduced pressure to obtain an extract, dry, grind to obtain an extract powder, add luteolin, and mix to obtain the extract.

[0057] Example 4

[0058] 4.1 Experimental Materials

[0059] Human oral cancer KB cells were purchased from the Cell Resource Center of the Chinese Academy of Sciences (Shanghai); fetal bovine serum, RPMI1640 culture medium, and trypsin were of Gibco brand; penicillin and streptomycin were (Beijing Solarbio Biotechnology Co., Ltd.); PBS solution was homemade according to the recipe; the samples to be tested were prepared using the pharmaceutical composition powders prepared in Examples 1, 2, 3 of the present invention and Comparative Example 1; the CCK-8 kit was purchased from Japanese colleagues; the brand of the AnnexinV-FITC / PI apoptosis detection kit was Solarbio.

[0060] 4.2 Experimental methods

[0061] 4.2.1 Cell recovery and subculture

[0062] Place human oral cancer KB cells in a 37°C water bath and gently shake to melt, mix with a pipette, transfer to a 15mL centrifuge tube, add an appropriate amount of complete culture medium (RPMI1640 medium containing 10% fetal bovine serum, 100U / mL penicillin, and 100μg / mL streptomycin) to suspend the cells, centrifuge, discard the supernatant, add an appropriate amount of complete culture medium to resuspend the cells, transfer to a cell culture bottle, place in a 37°C, 5% CO2 incubator, culture, when the cells fill the cell bottle, discard the culture medium, wash with PBS, digest the cells with 0.25% trypsin, centrifuge when the cell gap becomes larger, discard the supernatant, wash with PBS, add complete culture medium to inoculate in a new cell bottle, place in a 37°C, 5% CO2 incubator, and culture for passage. Collect well-growing logarithmic growth cells for experiments.

[0063] 4.2.2 Cell grouping and drug administration

[0064] (1) Negative control group: cultured with complete medium without drugs;

[0065] (2) Example 1 group: cultured in complete medium containing 5%, 15%, 25%, and 30% of the drug of Example 1, respectively;

[0066] (3) Example 2 group: cultured in complete medium containing 5%, 15%, 25%, and 30% of the drug of Example 1, respectively;

[0067] (4) Example 3 group: cultured in complete medium containing 5%, 15%, 25%, and 30% of the drug of Example 1, respectively;

[0068] (5) Comparative Example 1: The cells were cultured in complete culture medium containing 5%, 15%, 25%, and 30% of the drug of Example 1, respectively.

[0069] 4.2.3 Statistical analysis

[0070] SPSS 17.0 software was used for analysis. Data were expressed as mean ± standard deviation. The t test was used for comparison between groups, and one-way analysis of variance was used for comparison between multiple groups. P < 0.05 was considered statistically significant.

[0071] 4.2.4 Determination of cell apoptosis rate by flow cytometry

[0072] Human oral cancer cells in logarithmic growth phase were obtained and digested with 0.25% trypsin to prepare a cell suspension. The cell density was adjusted to 5×10 4 / mL, the cell suspension was transferred to a 96-well plate, 200 μL per well, and pre-cultured for 24 hours until the cells adhered well. Grouping was performed according to "4.2.2 Cell Grouping and Drug Administration" (only 15% and 25% concentrations were set for the drug group), 6 replicates per group, and after 48 hours of culture, 5 μL of PI and AnnexinV / FITC were added respectively under light-proof conditions. After 20 minutes, the apoptosis rate of human oral cancer KB cells was determined by flow cytometry in strict accordance with the steps in the operating instructions of the AnnexinV-FITC / PI apoptosis detection kit.

[0073] The results are shown in Table 1 and Figure 1 As shown in the results, it can be seen that the cell apoptosis rates of Examples 1, 2, and 3 groups are significantly higher than those of the negative control group and the comparative example 1 group, indicating that the pharmaceutical composition of the present invention has the activity of inducing apoptosis of human oral cancer KB cells.

[0074] Table 1 Cell apoptosis rate in each group

[0075] Group Cell apoptosis rate (%) Negative control 0.87±0.15 Example 1 Group - containing 15% 10.24±1.34 Example 1 Group - containing 25% 16.41±2.17 Example 2 Group - containing 15% 9.16±0.60 Example 2 Group - containing 25% 15.78±1.18 Example 3 Group - containing 15% 11.35±2.46 Example 3 Group - containing 25% 18.27±1.54 Comparative Example 1 - containing 15% 6.39±1.67 Comparative Example 1 - containing 25% 10.61±1.39

[0076] 4.2.5 CCK-8 assay to determine cell proliferation activity

[0077] Human oral cancer cells in logarithmic growth phase were obtained and digested with 0.25% trypsin to prepare a cell suspension. The cell density was adjusted to 5×10 4 / mL, the cell suspension was transferred to a 96-well plate, 200μL per well, and pre-cultured for 24h until the cells adhered well. Grouping was carried out according to "4.2.2 Cell Grouping and Drug Administration Culture", with 6 replicates per group. After 48h of culture, the original culture medium was aspirated and discarded, and 100μL of fresh culture medium and 10μL of CCK-8 reaction solution were added to each well. Incubation was carried out for 4h, and the absorbance value (OD) of each group at 450nm was measured by an ELISA instrument, and the inhibition rate of the drug on human oral cancer cells was calculated according to the following formula, and the concentration-inhibition rate curve was drawn. Inhibition rate = (1-OD drug group / OD negative control group) × 100%.

[0078] The results are shown in Table 2 and Figure 2 to Figure 5 As shown, it can be seen from the results that the groups of Examples 1, 2, and 3 can significantly inhibit the proliferation activity of human oral cancer KB cells, and this proliferation inhibition effect shows a relatively obvious dose-effect relationship.

[0079] Table 2 Proliferation inhibition rate of each group on human oral cancer KB cells

[0080]

[0081] 4.2.6 Transwell assay for cell migration activity

[0082] Human oral cancer cells in logarithmic growth phase were taken, digested and centrifuged, and then cultured with complete medium containing or without drugs for 48 h (the negative control group did not contain drugs, and the example group and the comparative group both contained 25% drugs), and then replaced with serum-free medium for starvation treatment for 12 h. After digestion and centrifugation, the cells were resuspended in serum-free medium and counted. 200 μL of cell suspension was added to each upper chamber, and each well contained 2×10 5 The whole chamber was placed in a 24-well plate containing complete culture medium and cultured for 24 h. The upper chamber was removed and fixed with anhydrous methanol, stained with crystal violet, washed 3 times with PBS, washed and air-dried, and observed under a fluorescent inverted microscope. Five fields of view were randomly selected under each microscope and counted under a high-power microscope to calculate the average value.

[0083] The results are shown in Table 3 and Figure 6 As shown in the results, it can be seen that the number of cell migration in the example group is significantly lower than that in the negative control group and the comparative example group, indicating that the pharmaceutical composition of the present invention has the activity of inhibiting the migration of human oral cancer KB cells.

[0084] Table 3 Number of migrating cells in each group

[0085] Group Negative control group Example 1 Group Example 2 Group Example 3 Group Comparative Example 1 Number of migrated cells 432.31±10.46 236.52±8.64 244.69±9.25 224.76±9.87 356.38±8.73

[0086] In summary, the pharmaceutical composition of the present invention can induce apoptosis of oral cancer cells, inhibit the proliferation of oral cancer cells, and inhibit the migration of oral cancer cells. It can be seen that the pharmaceutical composition of the present invention can be used for the treatment of oral cancer and has broad application prospects.

Claims

1. A pharmaceutical composition for treating oral cancer, characterized in that: The invention comprises the following raw materials in parts by weight: 10-40 parts by weight of American cockroach, 10-30 parts by weight of Solidago asiatica, 10-20 parts by weight of Radix Rehmanniae, 10-30 parts by weight of Scrophularia ningpoensis, 8-15 parts by weight of Herba Coicis, 10-20 parts by weight of Coix seeds and 0.5-2 parts by weight of luteolin.

2. The pharmaceutical composition according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 15-35 parts by weight of American cockroach, 10-20 parts by weight of Solidago asiatica, 15-20 parts by weight of Radix Rehmanniae, 10-25 parts by weight of Scrophularia ningpoensis, 9-13 parts by weight of Herba Coicis, 10-15 parts by weight of Coix seeds and 0.6-1.2 parts by weight of luteolin.

3. The method for preparing the pharmaceutical composition for treating oral cancer according to claim 1 or 2, characterized in that: The steps include: a. Crush the dried American cockroaches, soak them in an ethanol solution, reflux extract, concentrate until there is no alcohol taste, add water, mix well, filter, and obtain extract 1; b. Mix and grind the solidago asiatica, radix rehmanniae, rhizoma scrophulariae, rhizoma scrophulariae and coix seeds in the prescription, add water and boil, combine the decoctions, and filter to obtain extract 2; c. Combine the extract 1 and the extract 2, concentrate under reduced pressure to obtain an extract, dry, and grind into extract powder, add luteolin, and mix to obtain the extract.

4. The method for preparing the pharmaceutical composition for treating oral cancer according to claim 3, characterized in that: In step a, at least one of the following is satisfied: The concentration of the ethanol solution is 75% to 90%; The soaking time is 10 to 12 hours; Reflux extraction 2 to 3 times; the temperature of reflux extraction is 70 to 80°C; Concentrate until there is no alcohol taste, and add 5 to 8 times the amount of water as the raw material.

5. The method for preparing the pharmaceutical composition for treating oral cancer according to claim 3, characterized in that: In step b, add 9 to 12 times the total weight of Solidago asiatica, Radix Rehmanniae, Radix Scrophulariae, Radix Corydalis and Semen Coicis to boil for 2 to 3 times.

6. Use of the pharmaceutical composition according to claim 1 or 2 in the preparation of a drug for treating oral cancer.

7. The use according to claim 6, characterized in that: The pharmaceutical composition is used to inhibit the migration of oral cancer cells.

8. The use according to claim 6, characterized in that: The pharmaceutical composition is used to induce apoptosis of oral cancer cells.

9. The use according to claim 6, characterized in that: The pharmaceutical composition is used to inhibit the proliferation of oral cancer cells.

10. The use according to claim 6, characterized in that: The drug for treating oral cancer consists of the pharmaceutical composition according to claim 1 or 2 and a pharmaceutically acceptable carrier.

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