Mineralization-promoting high-strength magnesium phosphate bone cement as well as preparation and application thereof

Abstract

The invention relates to mineralization-promoting high-strength magnesium phosphate bone cement as well as preparation and application thereof. The bone cement solid-phase powder comprises the following components in parts by weight: 25-80 parts of trimagnesium phosphate, 10-50 parts of bioactive glass and 10-45 parts of dipotassium phosphate. And blending the bone cement solid-phase powder with pure water according to a solid-to-liquid ratio of 2-5g / mL to obtain the mineralization-promoting high-strength magnesium phosphate bone cement. Self-curing magnesium phosphate is used for loading high-strength bioactive glass powder, in the curing process, magnesium potassium phosphate gel formed by hydration of magnesium phosphate bone cement is used for coating the bioactive glass powder, and the high-strength bone repair scaffold is formed after curing; meanwhile, the mineralization promoting capability brought by the bioactive glass can make up for the deficiency of the mineralization promoting capability of the existing magnesium phosphate bone cement system. The preparation method is simple, the used raw materials are simple, and the method is suitable for mass production.

Description

Technical Field

[0001] The invention relates to a method in the technical field of biomedical materials, in particular to a mineralization-promoting high-strength magnesium phosphate bone cement and a preparation method and application thereof. Background Art

[0002] Osteoporosis (OP) is a common systemic bone metabolic disease characterized by low bone mass and bone microstructure damage, which can easily lead to non-traumatic fractures. Osteoporotic vertebral compression fractures (OVCFs) are the most common osteoporotic fractures, accounting for about 45% of osteoporotic fractures. Percutaneous vertebroplasty (PVP) is the most commonly used minimally invasive technique for the treatment of OVCFs. Currently, PMMA bone cement is commonly used as a filler in PVP surgery. This bone cement has high mechanical strength, but a large elastic modulus and does not have the biological activity of stimulating osteogenesis. Studies have shown that about 8% to 52% of OVCFs patients will suffer from adjacent vertebral compression fractures (AVF) after PVP. Calcium phosphate bone cement is a substitute for PMMA bone cement with good osteogenic activity, but its lower mechanical strength limits its further clinical application. Magnesium phosphate bone cement has the characteristics of fast solidification and high initial strength. Studies have shown that a certain concentration of extracellular magnesium ions can promote osteoblast activity and inhibit osteoclast activity. Therefore, magnesium phosphate bone cement has good biological activity and osteogenic ability, and has received widespread attention from researchers. However, as a filling material for hard tissue repair, magnesium phosphate bone cement still needs better mechanical properties and mineralization-promoting ability. At the same time, single magnesium phosphate bone cement cannot provide the calcium ions required for bone growth, and its osteogenic ability is limited.

[0003] Bioactive glass is a special type of glass that can form an amorphous calcium phosphate layer similar to the structure of bone material when in contact with biological tissue, thereby promoting the mineralization and regeneration of bones. Therefore, it has been widely used in the field of tissue engineering for bone repair. In view of the above background, the present invention provides a method for preparing a high-strength magnesium phosphate bone cement that promotes mineralization, and the potassium magnesium phosphate gel formed by the hydration of magnesium phosphate bone cement is used to coat the bioactive glass powder, and a high-strength bone repair scaffold is formed after solidification; at the same time, the mineralization promoting ability brought by the bioactive glass can make up for the shortcomings of the existing magnesium phosphate bone cement system. Summary of the invention

[0004] The invention aims to provide a mineralization-promoting high-strength magnesium phosphate bone cement.

[0005] Another object of the present invention is to provide a method for preparing the above-mentioned mineralization-promoting high-strength magnesium phosphate bone cement product.

[0006] Another object of the present invention is to provide an application of the above product.

[0007] The object of the present invention is achieved through the following scheme: a mineralization-promoting high-strength magnesium phosphate bone cement, comprising the following steps: 1) Bone cement solid phase powder, comprising the following components in parts by weight: 25-80 parts of trimagnesium phosphate, 10-50 parts of bioactive glass, and 10-45 parts of dipotassium hydrogen phosphate.

[0008] 2) The above-mentioned bone cement solid phase powder is blended with pure water at a solid-liquid ratio of 2-5 g / mL to obtain a mineralization-promoting high-strength magnesium phosphate bone cement.

[0009] The invention provides a preparation method of a mineralization-promoting high-strength magnesium phosphate bone cement. The trimagnesium phosphate and bioactive glass are homemade by the following method; the dipotassium hydrogen phosphate is anhydrous or trihydrate; and the solid phase powder is mixed by crushing and mixing in a dry environment using a crusher.

[0010] 1. Preparation of trimagnesium phosphate powder by solid phase reaction method: 1) Magnesium hydrogen phosphate trihydrate and magnesium hydroxide were mixed in a molar ratio of 2:1 in an ethanol medium at 300 rpm for 2-4 hours and dried at 60°C; 2) The mixed powder is calcined in a muffle furnace at 1150°C for 2-5 hours; 3) The sintered cake is ball-milled in an ethanol medium at 300-400 rpm for 4-18 hours and dried at 60° C. to obtain trimagnesium phosphate powder.

[0011] 2. Preparation of bioactive glass by solid phase reaction method: 1) Mix the raw materials of silicon dioxide, calcium carbonate, sodium carbonate and phosphorus pentoxide in a mass ratio of 45:24.5:24.5:6 to obtain a mixed powder, and the mixing method is dry mixing; 2) calcining the mixed powder at 1400°C for 2h to obtain molten glass slurry, which was then poured into water and rapidly cooled; 3) The product was dried at 100°C for 3 h, mechanically crushed, and passed through a 500-mesh screen to a particle size of 1-30 um to obtain bioactive glass.

[0012] The above-mentioned bone cement solid phase powder is blended with pure water at a solid-liquid ratio of 2-5 g / mL to obtain the mineralization-promoting high-strength magnesium phosphate bone cement.

[0013] The invention provides application of a mineralization-promoting high-strength magnesium phosphate bone cement in preparing a high-strength mineralization-promoting bone cement material.

[0014] The high-strength bioactive glass powder is loaded with self-curing magnesium phosphate, and during the curing process, the potassium magnesium phosphate gel formed by the hydration of magnesium phosphate bone cement is used to coat the bioactive glass powder, and a high-strength bone repair scaffold is formed after curing; at the same time, the mineralization-promoting ability brought by the bioactive glass can make up for the deficiency of the mineralization-promoting ability of the existing magnesium phosphate bone cement system. The preparation method of the present invention is simple, the raw materials used are simple, suitable for mass production, and has broad clinical application prospects.

[0015] The advantages of the present invention are: 1. The potassium magnesium phosphate gel formed by the hydration of magnesium phosphate bone cement is used to coat the bioactive glass powder, which forms a high-strength bone repair scaffold after solidification; the mineralization-promoting ability brought by the bioactive glass can make up for the shortcomings of the existing magnesium phosphate bone cement system.

[0016] 2. The preparation method of the present invention is simple, the raw materials used are simple, suitable for mass production, and has broad clinical application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is the compressive strength curve of the magnesium phosphate bone cement prepared in Example 3 of the present invention. DETAILED DESCRIPTION

[0018] The following examples are implemented based on the technical solutions of the invention, and provide detailed implementation methods and specific operation processes, but the protection scope of the present invention is not limited to the following examples.

[0019] Example 1 A mineralization-promoting high-strength magnesium phosphate bone cement is prepared according to the following steps: 1) Bone cement solid phase powder, comprising the following components by weight: 45 parts of trimagnesium phosphate, 10 parts of bioactive glass, and 45 parts of dipotassium hydrogen phosphate; crushing and mixing using a pulverizer in a dry environment to prepare a bone cement solid phase powder; wherein, Described trimagnesium phosphate is prepared by the following method: 120 g of magnesium hydrogen phosphate trihydrate and 20 g of magnesium hydroxide were ball-milled in an ethanol medium at 300 rpm for 2-4 hours, and dried at 60° C. to obtain a mixed powder; the mixed powder was calcined in a muffle furnace at 1150° C. for 5 hours, and the product was ball-milled in an ethanol medium at 400 rpm for 18 hours, and dried at 60° C. to obtain trimagnesium phosphate powder; The bioactive glass is prepared by the following method: 45g of silicon dioxide, 24.5g of calcium carbonate, 24.5g of sodium carbonate and 6g of phosphorus pentoxide were mixed evenly, calcined at 1400℃ for 2h, the molten glass slurry was poured into water and rapidly cooled, the product was dried at 100℃ for 3h, mechanically crushed, and passed through a 500-mesh sieve to obtain bioactive glass with a particle size of 1-30um; 2) The above-mentioned bone cement solid phase powder is blended with pure water at a solid-liquid ratio of 4 g / mL to obtain a mineralized high-strength magnesium phosphate bone cement.

[0020] The obtained mineralizing high-strength magnesium phosphate bone cement has an injectability of 95% and an average compressive strength of 32 MPa.

[0021] Example 2 A mineralization-promoting high-strength magnesium phosphate bone cement is prepared according to the following steps: 1) Bone cement solid phase powder, comprising the following components in parts by weight: 50 parts of trimagnesium phosphate, 25 parts of bioactive glass, and 25 parts of dipotassium hydrogen phosphate; crushing and mixing using a pulverizer in a dry environment to prepare a bone cement solid phase powder; 2) The above-mentioned bone cement solid phase powder is blended with pure water at a solid-liquid ratio of 4 g / mL to obtain a mineralized high-strength magnesium phosphate bone cement.

[0022] The obtained mineralizing high-strength magnesium phosphate bone cement has an injectability of 95% and an average compressive strength of 52 MPa.

[0023] Example 3 A mineralization-promoting high-strength magnesium phosphate bone cement is prepared according to the following steps: 1) Bone cement solid phase powder, comprising the following components in parts by weight: 30 parts of trimagnesium phosphate, 50 parts of bioactive glass, and 20 parts of dipotassium hydrogen phosphate; crushing and mixing using a pulverizer in a dry environment to prepare a bone cement solid phase powder; 2) The above-mentioned bone cement solid phase powder is blended with pure water at a solid-liquid ratio of 4 g / mL to obtain a mineralized high-strength magnesium phosphate bone cement.

[0024] The obtained high-strength magnesium phosphate bone cement for promoting mineralization has an injectability of 95%; the compressive strength curve of the magnesium phosphate bone cement prepared in this example is as follows Figure 1 As shown, the average compressive strength is 76MPa.

Claims

1. A method for preparing a mineralized high-strength magnesium phosphate bone cement, characterized in that: The following steps are involved: 1) Bone cement solid phase powder, comprising the following components in parts by weight: 25-80 parts of trimagnesium phosphate, 10-50 parts of bioactive glass, and 10-45 parts of dipotassium hydrogen phosphate; 2) The above-mentioned bone cement solid phase powder is blended with pure water at a solid-liquid ratio of 2-5 g / mL to obtain a mineralization-promoting high-strength magnesium phosphate bone cement.

2. The method for preparing the mineralized high-strength magnesium phosphate bone cement according to claim 1, characterized in that: In step 1), the trimagnesium phosphate is prepared according to the following steps: 1) Magnesium hydrogen phosphate trihydrate and magnesium hydroxide were mixed in a molar ratio of 2:1 in an ethanol medium by ball milling at 300 rpm for 2-4 hours, and dried at 60°C to obtain a mixed powder; 2) The mixed powder is calcined in a muffle furnace at 1150°C for 2-5 hours to obtain a sample product; 3) The sample product was ball-milled in an ethanol medium at 300-400 rpm for 4-18 h and dried at 60° C. to obtain trimagnesium phosphate powder.

3. The method for preparing the mineralized high-strength magnesium phosphate bone cement according to claim 1, characterized in that: In step 1), the bioactive glass is prepared according to the following steps: 1) dry-mixing raw materials of silicon dioxide, calcium carbonate, sodium carbonate and phosphorus pentoxide in a mass ratio of 45:24.5:24.5:6 to obtain a mixed powder; 2) calcining the mixed powder at 1400°C for 2h to obtain molten glass slurry, which was then poured into water and rapidly cooled; 3) The product was dried at 100°C for 3 h, mechanically crushed, and passed through a 500-mesh screen to a particle size of 1-30 um to obtain bioactive glass.

4. The method for preparing the mineralized high-strength magnesium phosphate bone cement according to claim 1, characterized in that: In step 1), the potassium hydrogen phosphate is anhydrous or trihydrate; the solid phase powder is mixed by crushing and mixing using a crusher in a dry environment.

5. Use of the mineralization-promoting high-strength magnesium phosphate bone cement according to any one of claims 1 to 4 in the preparation of high-strength mineralization-promoting bone cement materials.

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