Focusing optical filter and miniaturized fluorescence detection device

Abstract

The utility model discloses a focusing optical filter and a miniaturized fluorescence detection device. The focusing optical filter comprises at least one focusing part which is formed by injection molding of an injection molding material and color master batch, and the focusing part is a convex lens or a Fresnel lens and is used for filtering light with non-target wavelength in incident light and focusing light with target wavelength at the same time. The focusing optical filter is simple in structure and is only composed of the mounting part and the focusing part, and the mounting part is used for being connected with other external parts during fluorescence detection and protecting the focusing part. The focusing part is used for focusing the light and irradiating the light on the detector, so that photon points received by the detector are increased, and the detection sensitivity is improved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of fluorescence detection devices, and in particular to a focusing filter and a miniaturized fluorescence detection device. Background Art

[0002] Molecular POCT (point-of-care testing) is a technology that can be used for rapid analysis in clinics, homes, or laboratories. In the fields of medicine and biology, molecular POCT has become a hot technology that has attracted widespread attention in applications such as diagnosis and monitoring of diseases. By pursuing application scenarios such as home self-examination and graded diagnosis and treatment at home, molecular POCT devices have become one of the current research hotspots. Since molecular POCT is not performed in a laboratory, but in non-professional places (such as homes, clinics, etc.), the device has the characteristics of miniaturization, low cost, ease of use and portability.

[0003] At present, the detection sensitivity of molecular POCT devices is low. Therefore, there are still many challenges in realizing the high-sensitivity fluorescence detection of molecular POCT, and the required devices and accessories. For example, among the required accessories, filters play an important role. In existing molecular POCT devices, the filter used is made of glass. The glass surface is coated to produce a filtering effect. However, due to the poor plasticity of glass, the coating solution also needs to be attached to a flat surface, which limits the design of complex multifunctional filters. In addition, this kind of glass filter is expensive and difficult to process.

[0004] For example, a Chinese patent application (CN116500003A) discloses a handheld nucleic acid detection device, which includes a main body, which is used to be held by a user; the main body is provided with an installation cavity for accommodating a carrying module, the carrying module is used to accommodate a reaction tube, and the reaction tube is used to accommodate an object to be tested; the carrying module is connected to a heating module, and the heating module is used to adjust the temperature of the carrying module to adjust the temperature of the reaction tube to a first temperature; the main body is connected to an excitation light module, the excitation light module is arranged at the bottom of the carrying module, and the carrying module is provided with an excitation light inlet facing the excitation light module, so that the excitation light signal of the excitation light module is irradiated on the reaction tube through the excitation light inlet; the main body is also provided with a signal acquisition module, and the signal acquisition module is used to collect fluorescence signals of the fluorescence reaction of the reaction tube under the irradiation of the excitation light signal in real time and send them to the mobile terminal to obtain the detection result.

[0005] The signal acquisition module used in this patent application adopts a combination of an acquisition sensor and a filter. The filter adopts an ordinary acrylic plate filter. The detection sensitivity of the signal acquisition module is not high.

[0006] In addition, molecular diagnostic devices using traditional fluorescence detection technology must use expensive fluorescence detectors, which often makes the cost of the device higher, thereby hindering its development in low-cost scenarios.

[0007] Therefore, in the process of realizing low-cost, miniaturized molecular POCT devices, there is still a great demand for methods and devices that can improve the sensitivity of fluorescence detection. Utility Model Content

[0008] The present disclosure provides a focusing filter and a miniaturized fluorescence detection device to at least solve one of the technical problems existing in the prior art.

[0009] According to a first aspect of the present disclosure, there is provided a focusing filter, comprising a focusing portion formed by injection molding material and a masterbatch, wherein the focusing portion is provided with at least one convex lens or a Fresnel lens for filtering light of non-target wavelengths in incident light and focusing light of target wavelengths at the same time.

[0010] In one possible implementation manner, the focusing filter further comprises a mounting portion, a mounting hole is formed on the mounting portion, at least one mounting hole is provided and is arranged corresponding to the focusing portion, and the focusing portion is provided in the mounting hole.

[0011] In one possible implementation manner, the focusing portion has a first working surface and a second working surface that are arranged opposite to each other, and the first working surface and the second working surface both protrude from two ends of the mounting hole;

[0012] Or, one of the first working surface and the second working surface protrudes from one end of the mounting hole, and the other of the first working surface and the second working surface is located in the mounting hole;

[0013] Alternatively, neither the first working surface nor the second working surface protrudes beyond the two ends of the mounting hole.

[0014] In one possible implementation manner, when the first working surface and the second working surface both protrude from the two ends of the mounting hole, the first working surface and the second working surface are respectively connected to the adjacent side surfaces of the mounting portion to form a closed surface;

[0015] When one of the first working surface and the second working surface protrudes from one end of the mounting hole, the protruding working surface is connected to the adjacent side surface of the mounting portion to form a closed surface.

[0016] In one possible implementation, the focusing filter is an integrally formed part consisting of a focusing portion and a mounting portion.

[0017] In one possible implementation manner, the focusing filter is injection molded by mixing an injection molding material and a masterbatch.

[0018] According to a second aspect of the present disclosure, a miniaturized fluorescence detection device is provided, comprising a detector, an excitation light generating device and a reaction tube, and also comprising the focusing filter described in any one of the embodiments of the first aspect, wherein the focusing filter is installed in front of the detector, and the focusing filter is used to filter light of non-target wavelengths in the incident light and simultaneously focus light of target wavelengths to irradiate the detector.

[0019] In one embodiment, the distance between the focal point of the focusing filter and the detection surface of the detector is ±30% of the focal length of the focusing filter.

[0020] In one possible implementation manner, after the excitation light generated by the excitation light generating device irradiates the reaction tube, the incident light generated by the reaction tube enters the focusing portion of the focusing filter; wherein the angle between the incident light and the excitation light is 0° to 135°.

[0021] In one possible embodiment, the detector is a fluorescence sensor;

[0022] The angle between the incident light and the excitation light is 0° to 90°.

[0023] In one possible embodiment, the miniaturized fluorescence detection device is a miniaturized molecular POCT device.

[0024] Compared with the prior art, the advantages of the present application are: 1) The focusing filter of the present application can be formed by injection molding. Due to the strong plasticity of the injection molding shape, a filter with a very small focal length (1mm) can be realized; it has a wide range of application prospects in integrated, small and precise optical detection instruments. 2) The focusing filter of the present application has a flexible structure, and can achieve precise focusing without complex optical path design and clamping design; only through structural design, the focus can be placed on the detector to improve the detection sensitivity; 3) The focusing filter of the present application has a simple structure, consisting only of a mounting part and a focusing part. The mounting part is used to connect with other external parts during fluorescence detection and protect the focusing part. The focusing part is used to filter and focus the light and then irradiate it on the detector, increasing the number of photon points received by the detector, thereby improving the detection sensitivity. 4) The focusing filter of the present application is used for intelligent miniaturized fluorescence detection devices, which has the advantages of portability, easy operation and low cost. 5) The present application forms the required filtering effect by adjusting the masterbatch, liberating the flat structure required for coating, and allowing the shape of the filter to be realized completely according to the designer's ideas. 6) The material of the filter used in the present application is of high hardness and strong wear resistance, and the filtering effect will not be damaged by external environmental factors, so that the miniaturized molecular POCT device has a longer service life and is easier to maintain.

[0025] It should be understood that the content described in this section is not intended to identify the key or important features of the embodiments of the present disclosure, nor is it intended to limit the scope of the present disclosure. Other features of the present disclosure will become easily understood through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The above and other objects, features and advantages of the exemplary embodiments of the present disclosure will become readily understood by reading the detailed description below with reference to the accompanying drawings. In the accompanying drawings, several embodiments of the present disclosure are shown in an exemplary and non-limiting manner, in which:

[0027] In the drawings, the same or corresponding reference numerals represent the same or corresponding parts.

[0028] Figure 1 The structure of the first focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 1 ;

[0029] Figure 2 The structure of the first focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 2 ;

[0030] Figure 3 The structure of the first focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 3 ;

[0031] Figure 4 The structure of the first focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 4 ;

[0032] Figure 5 The structure of the second focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 1 ;

[0033] Figure 6 The structure of the second focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 2 ;

[0034] Figure 7 The structure of the second focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 3 ;

[0035] Figure 8 The structure of the third focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 1 ;

[0036] Fig. 9 The structure of the third focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 2 ;

[0037] Fig.10 The structure of the third focusing filter of the embodiment of the present disclosure is shown in FIG. Figure 3 ;

[0038] Fig.11 A schematic diagram showing the structure of an intelligent miniaturized fluorescence detection device in a first application scenario of an embodiment of the present disclosure is shown;

[0039] Fig.12 A partial structural schematic diagram of an intelligent miniaturized fluorescence detection device in a first application scenario of an embodiment of the present disclosure is shown;

[0040] Fig.13 Shows Fig.12 Disassembly diagram of

[0041] Fig.14 Shows Fig.12 Explosion hint Figure 1 ;

[0042] Fig.15 Shows Fig.12 Explosion hint Figure 2 ;

[0043] Fig.16 Shows Fig.12 Explosion hint Figure 3 ;

[0044] Fig.17 A schematic diagram showing the connection between a focusing filter and a carrier module of an intelligent miniaturized fluorescence detection device in a first application scenario of an embodiment of the present disclosure is shown;

[0045] Fig.18 The optical path of the intelligent miniaturized fluorescence detection device of the present disclosure embodiment is shown. Figure 1 ;

[0046] Fig.19 The optical path of the intelligent miniaturized fluorescence detection device of the present disclosure embodiment is shown. Figure 2 ;

[0047] Fig. 20 The optical path of the intelligent miniaturized fluorescence detection device of the present disclosure embodiment is shown. Figure 3 ;

[0048] Fig.21 The optical path of the intelligent miniaturized fluorescence detection device of the present disclosure embodiment is shown. Figure 4 ;

[0049] Fig. 22 A schematic diagram showing the structure of an intelligent miniaturized fluorescence detection device in a second application scenario of an embodiment of the present disclosure is shown;

[0050] Fig.23 A partial structural schematic diagram of an intelligent miniaturized fluorescence detection device in a second application scenario of an embodiment of the present disclosure is shown;

[0051] Fig.24 Shows Fig.23 Explosion hint Figure 1 ;

[0052] Fig.25 Shows Fig.23 Explosion hint Figure 2 ;

[0053] Fig.26 Shows Fig.23 Explosion hint Figure 3 .

[0054] Explanation of the numbers in the figure: focusing filter 1, focusing filter mounting plate 2, excitation light generating device 3, reaction tube 4, detector 5, carrying module 6, fluorescence sensor mounting substrate 7, background light 8, mounting part 11, focusing part 12, through hole 21, excitation light 31, mounting hole 111, first side surface 112, second side surface 113, first working surface 121, second working surface 122, reaction chamber 63, excitation light inlet 61, observation hole 62. DETAILED DESCRIPTION

[0055] In order to make the purpose, features, and advantages of the present disclosure more obvious and easy to understand, the technical solutions in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings in the embodiments of the present disclosure. Obviously, the described embodiments are only part of the embodiments of the present disclosure, not all of the embodiments. Based on the embodiments in the present disclosure, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present disclosure.

[0056] Based on the problem of poor detection sensitivity of existing miniaturized fluorescence detection devices, we accidentally discovered, after making improvements and adjustments in many aspects, that the focusing problem of the filter will lead to the sensitivity of the detection device. Based on this, the present application provides a focusing filter.

[0057] In a first aspect, according to an embodiment of the present disclosure, the utility model provides a focusing filter 1, such as Figure 1-10 A focusing filter 1 includes a focusing portion 12 formed by injection molding of an injection molding material and a masterbatch, wherein the focusing portion 12 is provided with at least one convex lens or a Fresnel lens, and is used to filter non-target wavelength light in incident light and simultaneously focus on target wavelength light.

[0058] The focusing filter 1 of the present application has a simple structure and can be composed of only a focusing part 12. The focusing part 12 is formed by injection molding material and masterbatch in a certain proportion, so that the focusing part 12 has a filtering function and can filter light of a specific wavelength (that is, filter light of non-target wavelengths in the incident light). Moreover, since the focusing part 12 formed by injection molding is a convex lens or a Fresnel lens, it can also focus the filtered incident light (that is, focus the light of the target wavelength in the incident light) at the same time.

[0059] In the present application, the injection molding material is a transparent injection molding material.

[0060] Since the focusing part 12 of the present application is a convex lens or a Fresnel lens, the convex lens can be divided into a plano-convex lens (one of the two working surfaces is a plane and the other is a convex surface), a biconvex lens (both working surfaces are convex surfaces) and a concave-convex lens (one of the two working surfaces is a convex surface and the other is a concave surface). The two working surfaces of the Fresnel lens, one of which is a plane, and the other is composed of a plurality of concentric circular curved surfaces. Therefore, the focusing part 12 has a first working surface 121 and a second working surface 122 that are arranged opposite to each other. Therefore, in the focusing filter 1 of the present application, at least one of the first working surface 121 and the second working surface 122 of the injection-molded focusing part 12 is a convex surface, and because a masterbatch is added during the injection molding, the injection-molded focusing part 12 has different colors and can filter light of different wavelengths. Therefore, the focusing part 12 of the focusing filter 1 of the present application has both the focusing function of a convex lens (ultra-short focal length focusing function, for example, the focal length of the focusing filter in the present application can be 1 to 2 mm) and the filtering function of a filter.

[0061] In the present application, light of non-target wavelengths includes but is not limited to excitation light, and light of target wavelengths includes but is not limited to fluorescence.

[0062] The focusing filter of the present application can be applied to a device for high-sensitivity fluorescence detection. For example, when the focusing filter 1 is applied to a miniaturized fluorescence detection device, the focusing portion 12 filters out the excitation light (light of non-target wavelength) on the one hand, and on the other hand focuses the emission light fluorescence (light of target wavelength) and irradiates it onto the detector 5, increasing the number of photon points received by the detector 5, thereby improving the detection sensitivity. This makes the focusing filter 1 have a wide range of application prospects in integrated, small and precise optical detection instruments.

[0063] In one embodiment, the focusing filter 1 of the present application further includes a mounting portion 11, the mounting portion 11 is provided with a through mounting hole 111, the mounting hole 111 is provided with at least one and is arranged corresponding to the focusing portion 12, and the focusing portion 12 is arranged in the mounting hole 111. The mounting portion 11 is used to connect the focusing filter 1 with other external parts when it is used in a specific application.

[0064] For example, when the focusing portion 12 is disposed in the mounting hole 111, there may be different configurations:

[0065] 1) One of the first working surface 121 and the second working surface 122 protrudes from one end of the mounting hole 111 , and the other of the first working surface 121 and the second working surface 122 is located in the mounting hole 111 .

[0066] like Figure 1-4 As shown, the first working surface 121 protrudes from one end of the mounting hole 111 and is connected to the first side surface 112 of the adjacent mounting portion 11 to form a closed surface. The second working surface 122 is located in the mounting hole 111 to protect the second working surface 122 .

[0067] 2) The first working surface 121 and the second working surface 122 both protrude from two ends of the mounting hole 111 .

[0068] like Figure 5-7 As shown, the mounting portion 11 is a relatively thin plate-shaped structure, and the two working surfaces of the focusing portion 12 are both protruding from the two ends of the mounting hole of the mounting portion 11 .

[0069] Furthermore, it can be seen from FIGS. 5-7 that the first working surface 121 and the second working surface 122 of the focusing portion 12 are respectively connected to the first side surface 112 and the second side surface 113 of the adjacent mounting portion to form a closed surface.

[0070] 3) The first working surface 121 and the second working surface 122 do not protrude from both ends of the mounting hole 111 .

[0071] like Figure 8-10 As shown, the first working surface 121 and the second working surface 122 of the focusing part 12 are both located in the mounting hole 111 and do not protrude from the two open ends of the mounting hole 111, so that when the mounting part 11 is connected with other components, it will not interfere with other components and damage the working surface.

[0072] It can be seen from the above three settings that the shape of the mounting portion 11 is generally a plate-like structure, and the side surface of the mounting portion where the two open ends of the mounting hole 111 are located is flat, which is convenient for connection and installation with other components. The thickness of the mounting portion 11 and the size of the mounting hole 111 can be adaptively adjusted according to the size of the focusing portion 12.

[0073] The focusing filters 1 with the three different configurations mentioned above can all be used in a fluorescence detection device to improve the detection sensitivity of the detection device.

[0074] Furthermore, the focusing filter 1 of the present application is an integrally formed part formed by the focusing part 12 and the mounting part 11. The integrally formed part is easy to produce and saves costs.

[0075] In the present application, the focusing filter 1 can be produced in one piece by mixing the injection molding material with the masterbatch through the injection molding process, so that the focusing filter 1 has plasticity, and because the masterbatch is added to the injection molding material to produce a filtering effect, a focusing filter is produced. The focusing filter can filter light of a specific wavelength and focus the filtered light at the same time. Through the injection molding process, focusing filters of various shapes and functions can be produced. Compared with the current glass-based filters, more functions can be achieved, and the cost of injection molding is lower, and the potential for mass production is greater. For example, the focusing filter material (injection molding material) is one of PMMA, PC, PET, PETG, PS, PU, ​​TPU, LSR, and transparent nylon. Among them, the desired filtering effect is formed by adjusting the masterbatch, which liberates the existing glass coating from having to have a flat structure, and the shape of the filter can be completely realized according to the designer's idea.

[0076] The injection molding material includes transparent plastics, which include but are not limited to: PMMA (polymethyl methacrylate), PC (polycarbonate), PET (polyethylene terephthalate), PETG (polyethylene terephthalate-1,4-cyclohexanedimethanol ester), PS (polystyrene), PU (polyurethane), TPU, LSR (liquid silicone rubber) and one of transparent nylon.

[0077] The focusing filter material used in the present application has high hardness and strong wear resistance, and the filtering effect will not be damaged by external environmental factors, so that the detection device has a longer service life and is easier to maintain.

[0078] Alternatively, the focusing portion 12 and the mounting portion 11 are separate parts, for example, the focusing portion 12 and the mounting portion 11 are injection molded separately, and the focusing portion 12 and the mounting portion 11 can be fixed by bonding.

[0079] In the present application, when the focusing filter 1 is injection molded, the masterbatch accounts for 2 to 10 wt% of the injection molding material, and the injection molding temperature is 250 to 350° C. The masterbatch accounts for 2 to 10 wt% of the injection molding material, and the prepared focusing filter 1 has a good filtering effect. Preferably, the masterbatch accounts for 2 to 5 wt% of the injection molding material.

[0080] Masterbatch is composed of pigment and solvent and is widely used in the plastic industry. The masterbatch used in this application can be obtained through commercial channels. The principle of masterbatch filtering incident light is that the pigment dispersed in the focusing filter has strong absorption of excitation light and weak absorption of emitted light fluorescence.

[0081] When the focusing filter 1 is actually applied to a device based on fluorescence detection, when the focusing filter 1 filters the excitation light and simultaneously focuses the emitted light fluorescence onto the detection surface of the detector, the smaller the masterbatch ratio is, the greater the detection value is after the focusing filter 1 formed by injection molding filters the incident light and focuses it onto the detection surface of the detector. The greater the masterbatch ratio is, the smaller the detection value of the corresponding detector is. Therefore, when the masterbatch accounts for 2 to 10 wt% of the injection molding material, the detection value of the corresponding detector is 15000 to 500.

[0082] In the second aspect, according to an embodiment of the present disclosure, the utility model also provides a miniaturized fluorescence detection device, including a detector 5, an excitation light generating device 3 and a reaction tube 4, and also includes a focusing filter 1 in any one of the embodiments in the first aspect, the focusing filter 1 is installed in front of the detector 5, and the focusing filter 1 is used to filter the light of non-target wavelengths in the incident light and simultaneously focus the light of the target wavelength on the detector 5. Specifically, the focusing filter filters the excitation light in the incident light and simultaneously focuses the emission light fluorescence on the detector 5. The miniaturized fluorescence detection device with the focusing filter 1 can focus the emission light fluorescence onto the detection surface of the detector while filtering out the excitation light, thereby increasing the photon points received by the detector, thereby improving the detection sensitivity. In addition, the present application simplifies the optical path design, which is conducive to the use of precision instruments. For example, the miniaturized fluorescence detection device can be a miniaturized molecular POCT device.

[0083] When the focusing filter 1 is applied to a miniaturized fluorescence detection device, after the excitation light generated by the excitation light generating device 3 is irradiated on the reaction tube 4, the incident light generated by the reaction tube 4 enters the focusing portion 12 of the focusing filter 1. At this time, the incident light contains two kinds of light: excitation light (light of non-target wavelength) and emission light fluorescence (light of target wavelength). Therefore, after the incident light enters the focusing portion, the focusing portion filters the excitation light and simultaneously focuses the emission light fluorescence to irradiate the detector, thereby improving the detection sensitivity of the detector.

[0084] For example, the arrangement distance between the detector 5 and the focusing filter 1 may be: the distance between the focus of the focusing filter 1 and the detection surface of the detector 5 is ±30% of the focal length of the focusing filter 1. Specifically, when the distance between the focus and the detection surface is between -30% and 0% of the focal length, it means that the detection surface of the detector is located in front of the focus, and the focusing portion of the focusing filter 1 has converged the emitted light fluorescence but has not yet converged to the focus, and at this distance, the photon points received by the detection surface of the detector 5 can also be greatly increased.

[0085] When the distance between the focus and the detection surface is between 0% and 30% of the focal length, it means that the detection surface of the detector 5 is located behind the focus, and the focusing part 12 has converged the emitted light fluorescence to the focus and the emitted light fluorescence is in a partially dispersed state. This distance can also increase the photon points received by the detection surface of the detector.

[0086] Of course, the best situation is that the detection surface of the detector 5 is located at the focus. This installation distance can maximize the increase of photon points received by the detection surface of the detector, thereby maximizing the detection sensitivity. Therefore, the focus and focal length of the focusing unit 12 can be adjusted according to actual conditions.

[0087] After the excitation light generated by the excitation light generating device 3 is irradiated on the reaction tube 4, the incident light generated by the reaction tube 4 enters the focusing portion 12 of the focusing filter 1; wherein the angle between the incident light and the excitation light is 0° to 135°. For example, the detector is a fluorescence sensor; the angle between the incident light and the excitation light is 0° to 90°; the angle between the incident light and the excitation light is 30°.

[0088] The specific application scenarios of the focusing filter of the present application are given below. The focusing filter of the present application can replace the existing glass filter, thereby improving the detection sensitivity.

[0089] Example 1

[0090] This embodiment 1 applies the focusing filter 1 in the published Chinese patent application (CN 116500003A). By replacing the existing filter in the device with the focusing filter 1 of the present application, the detection sensitivity of the device can be improved.

[0091] like Figure 11-16 As shown, an intelligent miniaturized fluorescence detection device is a miniaturized handheld nucleic acid detection device.

[0092] The intelligent miniaturized fluorescence detection device includes a detector 5, an excitation light generating device 3, a reaction tube 4, a focusing filter 1, a focusing filter mounting plate 2, and a carrier module 6. The focusing portion 12 is formed by injection molding of an injection molding material and a masterbatch, so that the focusing portion 12 is a double convex lens formed by a first working surface 121 and a second working surface 122. The focusing portion 12 is arranged in the mounting hole 111 of the mounting portion 11, so that the first working surface 121 protrudes from one of the open ends of the mounting hole, and is connected to the first side surface 112 of the adjacent mounting portion to form a closed surface, and the second working surface 122 is located in the mounting hole 111; the focusing portion 12 and the mounting portion 11 are integrally formed. The excitation light generating device 3 is arranged at the bottom of the carrier module 6, such as Figure 14-15 and Fig.18As shown, a reaction chamber 63 is provided in the carrier module 6, and the reaction chamber 63 is used to accommodate the reaction tube 4, and the carrier module 6 is provided with an excitation light inlet 61 connected with the reaction chamber 63 toward the excitation light generating device 3, so that the excitation light signal of the excitation light generating device 3 is irradiated on the reaction tube 4 through the excitation light inlet 61. A heating module is provided on one side of the carrier module 6, and an observation hole 62 is opened on the carrier module 6, and the observation hole 62 is connected with the reaction chamber 63. The observation hole 62 is arranged away from the heating module and a focusing filter mounting plate 2 is installed on the observation hole side.

[0093] A through hole 21 is provided on the focusing filter mounting plate 2. The focusing filter 1 is mounted on the through hole 21 of the focusing filter mounting plate 2 through the mounting portion 11, so that the second working surface 122 of the focusing portion 12 of the focusing filter 1 is arranged corresponding to the observation hole 62. The detector 5 is connected to the focusing filter mounting plate 2, so that the focusing filter mounting plate 2 is located between the detector 5 and the carrier module 6. The detector 5 corresponds to the first working surface 121 of the focusing portion 12 of the focusing filter 1 and is arranged close to the focusing portion 12. The excitation light generated by the excitation light generating device 3 is irradiated on the reaction tube 4 through the excitation light inlet 61, and the incident light generated after the reactants in the reaction tube 4 are excited is irradiated on the second working surface 122 of the focusing filter 1 through the observation hole 62. The focusing portion 12 of the focusing filter 1 is used to filter out the excitation light so that the emission light fluorescence passes through, and the emission light fluorescence is focused and irradiated on the detection surface of the detector 5, increasing the photon points received by the detector 5, thereby improving the detection sensitivity. The angle between the incident light entering the focusing filter 1 and the excitation light 31 is 90°. Fig.18 shown.

[0094] Among them Figure 11-16 In the embodiment, the focusing portion 12 of the focusing filter 1 has two working surfaces, wherein the first working surface 121 protrudes out of the mounting hole 111 of the mounting portion 11, and the second working surface 122 is located in the mounting hole 111. The protruding first working surface 121 is arranged corresponding to the detector.

[0095] Of course, the focusing filter can also be turned over so that the first working surface 121 is arranged corresponding to the observation hole 62, such as Fig.17 As shown, after the focusing filter mounting plate 2 is connected to the carrier module 6, the first working surface 121 of the focusing portion 12 of the focusing filter 1 is located in the observation hole 62 of the carrier module 6, so that the mounting portion and the side of the carrier module are in contact. The side of the mounting portion 11 of the focusing filter 1 can be in contact with the detector 5, so that the focusing portion 12 focuses the light, so that the focus is just irradiated on the detection surface of the detector, thereby enhancing the light effect and improving the detection sensitivity.

[0096] The detector is a fluorescence sensor; since the focusing filter is made by injection molding, it has a certain plasticity, so the structure is more flexible, and does not require complex optical path design, and precise focusing can be achieved without clamping design. Therefore, different excitation light paths can be designed to meet different needs.

[0097] In addition, the excitation light generating device 3 can be arranged at other positions besides the bottom of the reaction tube 4:

[0098] like Fig.19 As shown in the figure; the excitation light generating device 3 is arranged at the bottom of the reaction tube, the focusing filter 1 and the detector 5 are both arranged on the side of the reaction tube 4, and the focusing filter 1 and the excitation light generating device 3 are arranged at an angle of 30°. The excitation light 31 generated by the excitation light generating device 3 irradiates the reaction tube 4 from the bottom, and the incident light generated after the reactants in the reaction tube 4 are excited passes through the focusing filter 1. The detector 5 is arranged on the side of the focusing filter 1. The focusing filter 1 filters the excitation light in the incident light and focuses the emitted light fluorescence and then irradiates it on the detection surface of the detector 5; the angle between the incident light passing through the focusing filter and the excitation light 31 is 30°.

[0099] like Fig. 20 As shown; the excitation light generating device 3 and the focusing filter 1 are both arranged at the top of the reaction tube 4, the generated excitation light 31 is irradiated on the reaction tube 4 from the top, the focusing filter 1 and the excitation light 31 are arranged in parallel, the incident light generated after the reactants in the reaction tube are excited passes through the focusing filter 1, and the detector 5 is arranged on the side of the focusing filter 1. The focusing filter 1 filters the excitation light in the incident light and focuses the emitted light fluorescence and then irradiates it on the detection surface of the detector 5; the angle between the incident light passing through the focusing filter and the excitation light 31 is 0°.

[0100] like Fig.21 As shown, the excitation light generating device 3 is arranged at the top of the reaction tube, the excitation light 31 is irradiated on the reaction tube 4 from the top, the focusing filter 1 is arranged on the side of the reaction tube 4, the focusing filter 1 and the excitation light are arranged at an angle of 90°, the incident light generated after the reactants in the reaction tube 4 are excited passes through the focusing filter 1, and the detector 5 is arranged on the side of the focusing filter 1. The focusing filter 1 filters the excitation light in the incident light and focuses the emitted light fluorescence and then irradiates it on the detection surface of the detector 5; the angle between the incident light passing through the focusing filter 1 and the excitation light is 90°.

[0101] Example 2

[0102] This embodiment 2 applies the focusing filter 1 to the published Chinese patent application (CN115404159A). On this basis, the detection sensitivity of the device is improved by adding the focusing filter 1 and the fluorescence sensor 5.

[0103] like Figure 22-26 As shown, this embodiment 2 provides an intelligent miniaturized fluorescence detection device.

[0104] An intelligent miniaturized fluorescence detection device includes a carrier module 6, a reaction tube 4, a control module and an excitation light generating device 3, wherein the excitation light generating device 3 is arranged at the bottom of the carrier module 6. A reaction chamber is arranged in the carrier module 6, and the reaction chamber is used to accommodate the reaction tube 4. The carrier module 6 is provided with an excitation light inlet 61 connected with the reaction chamber toward the excitation light generating device 3, so that the excitation light signal of the excitation light generating device 3 is irradiated on the reaction tube 4 through the excitation light inlet 61. It also includes a focusing filter mounting plate 2, a fluorescence sensor mounting substrate 7, a fluorescence sensor 5, a background light 8 and a focusing filter 1. The background light 8 and the fluorescence sensor 5 are both connected to the control module.

[0105] The carrying module 6 is provided with eight observation holes 62 along its length direction, and the focusing filter mounting plate 2 is provided with eight through holes 21 along its length direction, and a focusing filter 1 is mounted in each through hole 21, so that one of the working surfaces of the focusing part 12 is arranged in one-to-one correspondence with the observation hole 62. The fluorescence sensor mounting substrate 7 is fixedly mounted on the side of the focusing filter mounting plate 2, and eight fluorescence sensors 5 are mounted on the fluorescence sensor mounting substrate 7 and arranged in one-to-one correspondence with the other working surface of the focusing part 12.

[0106] The excitation light generated by the excitation light generating device 3 is irradiated onto the reaction tube 4 through the excitation light inlet 61. The incident light generated after the reactants in the reaction tube 4 are excited is irradiated onto the working surface of the focusing filter 1 through the observation hole 62. The focusing part of the focusing filter 1 is used to filter out the excitation light so that the emission light fluorescence passes through, and the emission light fluorescence is focused and irradiated onto the photosensitive surface of the fluorescence sensor 5. The fluorescence sensor 5 quantitatively detects the fluorescence signal of the reaction tube in real time and transmits the fluorescence signal to the control module. After receiving the fluorescence signal, the control module processes it to obtain the detection result, and controls and adjusts the light color of the background light 8 to display the current detection result.

[0107] The intelligent miniaturized fluorescence detection device with the focusing filter 1 can also filter the excitation light and simultaneously focus the emitted light fluorescence onto the fluorescence sensor, thereby improving the detection sensitivity.

[0108] It should be understood that the various forms of processes shown above can be used to reorder, add or delete steps. For example, the steps recorded in this disclosure can be executed in parallel, sequentially or in different orders, as long as the desired results of the technical solutions disclosed in this disclosure can be achieved, and this document does not limit this.

[0109] In addition, the terms "first" and "second" are used only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present disclosure, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined. In the description of the present application, it should be understood that the orientation or position relationship indicated by the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present application.

[0110] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present disclosure. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification and features of different embodiments or examples, unless they are contradictory.

[0111] The above is only a specific embodiment of the present disclosure, but the protection scope of the present disclosure is not limited thereto. Any person skilled in the art who is familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed in the present disclosure, which should be included in the protection scope of the present disclosure. Therefore, the protection scope of the present disclosure should be based on the protection scope of the claims.

Claims

1. A focusing filter, characterized in that: The focusing part comprises a focusing part formed by injection molding of an injection molding material and a masterbatch, wherein the focusing part is at least provided with a convex lens or a Fresnel lens, and is used for filtering light of non-target wavelengths in incident light and focusing light of target wavelengths at the same time.

2. The focusing filter according to claim 1, characterized in that: The focusing filter also includes a mounting portion, a mounting hole is formed on the mounting portion, at least one mounting hole is provided and is arranged corresponding to the focusing portion, and the focusing portion is arranged in the mounting hole.

3. The focusing filter according to claim 2, characterized in that: The focusing portion has a first working surface and a second working surface that are arranged opposite to each other, and the first working surface and the second working surface both protrude from two ends of the mounting hole; Or, one of the first working surface and the second working surface protrudes from one end of the mounting hole, and the other of the first working surface and the second working surface is located in the mounting hole; Alternatively, neither the first working surface nor the second working surface protrudes beyond the two ends of the mounting hole.

4. The focusing filter according to claim 3, characterized in that: When the first working surface and the second working surface both protrude from the two ends of the mounting hole, the first working surface and the second working surface are respectively connected to the adjacent side surfaces of the mounting portion to form a closed surface; When one of the first working surface and the second working surface protrudes from one end of the mounting hole, the protruding working surface is connected to the adjacent side surface of the mounting portion to form a closed surface.

5. The focusing filter according to any one of claims 1 to 4, characterized in that: The focusing filter is an integrally formed part consisting of a focusing part and a mounting part.

6. The focusing filter according to claim 5, characterized in that: The focusing filter is formed by mixing injection molding material and masterbatch.

7. A miniaturized fluorescence detection device, comprising a detector, an excitation light generating device and a reaction tube, characterized in that: It also includes the focusing filter described in any one of claims 1-6, wherein the focusing filter is installed in front of the detector, and is used to filter light of non-target wavelengths in the incident light and simultaneously focus the light of target wavelength to irradiate the detector.

8. The miniaturized fluorescence detection device according to claim 7, characterized in that: The distance between the focal point of the focusing filter and the detection surface of the detector is ±30% of the focal length of the focusing filter.

9. The miniaturized fluorescence detection device according to claim 8, characterized in that: After the excitation light generated by the excitation light generating device irradiates the reaction tube, the incident light generated by the reaction tube enters the focusing portion of the focusing filter; wherein the angle between the incident light and the excitation light is 0° to 135°.

10. The miniaturized fluorescence detection device according to claim 9, characterized in that: The detector is a fluorescence sensor; The angle between the incident light and the excitation light is 0° to 90°.

11. The miniaturized fluorescence detection device according to any one of claims 7 to 10, characterized in that: The miniaturized fluorescence detection device is a miniaturized molecular POCT device.

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