Microorganism outer membrane vesicle preparation device

Abstract

The utility model discloses a microbial outer membrane vesicle preparation device, and relates to the technical field of outer membrane vesicle preparation, the microbial outer membrane vesicle preparation device comprises a preparation box, the left side of the upper surface of the inner cavity of the preparation box is rotatably connected with a centrifugal assembly, and the middle part of the upper surface of the inner cavity of the preparation box is fixedly provided with a suction pump; the output end and the input end of the suction pump are fixedly connected with telescopic hoses, and a filtering assembly is fixedly installed on the right side of the lower surface of an inner cavity of the preparation box. The motor drives the first rotating shaft and the first gear to rotate, the first gear drives the second gear, the second rotating shaft, the connecting shaft and the centrifugal barrel to rotate intermittently, so that microorganism outer membrane vesicle fermentation liquor can be centrifuged, the centrifugal barrel can vibrate through the protruding blocks, the sliding rods, the balls and the limiting grooves, the centrifugal effect of the vesicle fermentation liquor is improved, the connecting shaft is rotated, and the rotating speed is increased. And the inserting rod is taken down from the first inserting groove in the connecting shaft, the second rotating shaft and the connecting shaft can be detached, and sediment in the centrifugal barrel can be conveniently cleaned.

Description

Technical Field

[0001] The utility model relates to the technical field of outer membrane vesicle preparation, in particular to a device for preparing microbial outer membrane vesicles. Background Art

[0002] Outer membrane vesicles (OMVs) are functional vesicles that are constantly detached from the bacterial cell surface during bacterial life activities. They contain proteins, lipids, nucleic acids and other components, have a variety of special biological functions, and play a key role in bacteria-bacteria and bacteria-host interactions. Changes in the microbial population (i.e., imbalance) disrupt the balance of the human immune system and increase the likelihood of human disease. Antimicrobial agents synthesized using microbial outer membrane vesicles are widely used in clinical treatment, so a preparation device is needed to prepare microbial outer membrane vesicles.

[0003] For example, the Chinese patent with publication number CN115029258A discloses a microbial outer membrane vesicle and its preparation, preservation method and application, which uses its nano size to enter the stratum corneum to stimulate keratinocytes to enhance the expression of antimicrobial peptides and reduce the risk of atopic dermatitis infection; it can also be used to treat atopic dermatitis, fight skin inflammation, infection and wounds, etc. It can also be used as a nutrient component, absorbed and utilized by Staphylococcus epidermidis, promote the proliferation of beneficial bacteria, has the inherent properties of nanomaterials, can be loaded with small molecule drugs inside, and can be used as a nanocarrier in drug delivery systems: it is also more convenient in storage, transportation and use. It can be mass-produced by fermentation, and compared with the direct application of live bacteria, outer membrane vesicles have better biosafety. It can be targeted to specific parts and can also be used as a vaccine.

[0004] The existing technology has the following problems:

[0005] During the preparation process of the above-mentioned microbial outer membrane vesicles, when the outer membrane vesicle fermentation broth is centrifuged, the centrifugal effect is general, and it is inconvenient to operate the centrifugal mechanism, and thus it is inconvenient to remove the precipitate. At the same time, when the fermentation broth is filtered using a filter membrane, the filter pores on the filter membrane are often clogged, and it is inconvenient to disassemble and replace the filter membrane, resulting in the outer membrane vesicles being squeezed and destroyed during screening and filtration. Utility Model Content

[0006] The utility model provides a device for preparing microbial outer membrane vesicles to solve the problems raised in the above background technology.

[0007] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0008] A device for preparing microbial outer membrane vesicles comprises a preparation box, wherein a centrifugal assembly is rotatably connected to the left side of the upper surface of the inner cavity of the preparation box, a suction pump is fixedly installed in the middle of the upper surface of the inner cavity of the preparation box, the output end and the input end of the suction pump are both fixedly connected to a telescopic hose, and a filtering assembly is fixedly installed on the right side of the lower surface of the inner cavity of the preparation box.

[0009] The centrifugal assembly includes eight protrusions, a second rotating shaft and a motor. The top end of the second rotating shaft is rotatably connected to the left side of the upper surface of the inner cavity of the preparation box, the lower surface of the motor is fixedly installed to the left side of the upper surface of the preparation box, the output end of the motor extends to the inner cavity of the preparation box and is fixedly connected to the first rotating shaft, the lower end of the second rotating shaft is threadedly connected to a connecting shaft, the outer surface of the connecting shaft is movably sleeved with a centrifugal bucket, and the upper surface of the centrifugal bucket is clamped with two arc-shaped pieces.

[0010] The filter assembly includes a filter box, the lower surface of which is fixedly installed on the right side of the lower surface of the inner cavity of the preparation box, the inner cavity of the filter box is fixedly connected with a connecting frame, the upper surface of the connecting frame is overlapped with a cover plate 1, a screening filter membrane and a cover plate 2 are arranged above the cover plate 1, an injection port and a liquid suction port are respectively opened on the left and right sides of the upper surface of the cover plate 2, and a liquid discharge port is opened in the middle of the upper surface of the cover plate 1.

[0011] Preferably, the outer surface of the rotating shaft 2 is fixedly connected to gear 2, the bottom end of the outer surface of the rotating shaft 1 is fixedly connected to gear 1, and the teeth of gear 1 are half of those of gear 2, the left side of gear 2 is meshed with the right side of gear 1, and two arc-shaped pieces are clamped on the upper surface of the centrifugal barrel.

[0012] Preferably, a slot 1 is provided on the left side of the outer surface of the connecting shaft near the top, an insertion rod passing through the rotating shaft 2 is inserted into the inner wall of the slot 1, and the slot 1 is a cylindrical structure.

[0013] Preferably, the inner wall of the centrifugal barrel axis is rotatably connected with five balls, and the five balls are distributed in three groups in a linear array. A limiting groove is provided on the outer surface of the connecting shaft, and the surface of the limiting groove is slidably connected to the outer surface of the ball.

[0014] Preferably, eight sliding rods are fixedly connected to the lower surface of the centrifugal barrel, and the eight sliding rods are distributed in a circular array, the upper surfaces of the eight protrusions are fixedly connected to the left side of the lower surface of the inner cavity of the preparation box, and the eight protrusions are distributed in a circular array along the axis of the centrifugal barrel, the outer surface of the sliding rod is slidably connected to the surface of the protrusion, and the protrusion has a semi-arc structure.

[0015] Preferably, the upper surface of the cover plate one is fixedly connected with a sealing ring one, and the lower surface of the cover plate two is fixedly connected with a sealing ring two, the lower surface of the sealing ring two and the upper surface of the sealing ring one overlap with the upper and lower surfaces of the screening filter membrane respectively, and the sealing ring one and the sealing ring two are both rectangular structures.

[0016] Preferably, a slot two is provided in the middle of the cover plate two and the left and right sides of the cover plate one, and slots are provided on the left and right side surfaces of the inner cavity of the filter box, a spring is fixedly connected to the side of the inner cavity of the slot away from the cover plate two, and the other end of the spring is fixedly connected to an insertion block, and the outer surface of the insertion block is plugged into the inner wall of the slot two, and the inner walls on the left and right sides of the filter box are movably connected with push rods, one end of the push rods is fixedly connected to the side of the slot two away from the cover plate two, and the slot two and the side of the insertion block close to the cover plate two are conical structures.

[0017] Preferably, a positioning frame is fixedly connected to the lower surface of the cover plate 1, a positioning groove is opened on the upper surface of the connection frame, the inner wall of the positioning groove is plugged into the outer surface of the positioning frame, and the positioning frame and the positioning groove are both rectangular structures.

[0018] Due to the adoption of the above technical solution, the utility model has achieved the following technical progress compared with the prior art:

[0019] The utility model provides a device for preparing microbial outer membrane vesicles. A motor drives a rotating shaft and a gear to rotate. The gear drives a gear, a rotating shaft, a connecting shaft and a centrifugal bucket to rotate intermittently, so that the microbial outer membrane vesicle fermentation liquid can be centrifuged. The centrifugal bucket can be vibrated by means of a convex block, a sliding rod, a ball and a limiting groove, so that the centrifugal effect of the vesicle fermentation liquid is improved, thereby improving the preparation quality of the finished microbial outer membrane vesicles. The connecting shaft is rotated to remove the insertion rod from a slot one on the connecting shaft, and the rotating shaft and the connecting shaft can be disassembled, so that the sediment in the centrifugal bucket can be cleaned up.

[0020] The utility model provides a microbial outer membrane vesicle preparation device, under the action of the flow force flowing from the injection port to the liquid suction port, the vesicle fermentation liquid flows laterally, so that the vesicle fermentation liquid on the surface of the screening filter membrane is affected by the flow force flowing to the discharge port and the flow force flowing to the liquid suction port, so that the vesicle fermentation liquid can pass through the screening filter membrane to prevent the filter holes from being blocked, thereby reducing the probability of the filter holes being blocked and the vesicles being squeezed and destroyed during vesicle screening, and the cover plate two and the cover plate one can be disassembled by moving the push rod, the spring and the plug block, so that the screening filter membrane can be replaced, and the sealing ring two and the sealing ring one with rectangular structures can be set to prevent the vesicle fermentation liquid from leaking during filtration. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural schematic diagram of the utility model;

[0022] Figure 2 This is a schematic diagram of the fermentation component structure of the utility model;

[0023] Figure 3 This is a schematic diagram of the cross-sectional structure of a fermentation barrel of the present invention;

[0024] Figure 4 It is a schematic diagram of the enlarged structure of point A of the utility model;

[0025] Figure 5 It is an enlarged structural diagram of B of the utility model;

[0026] Figure 6 This is a schematic diagram of the structure of the filter assembly of the utility model;

[0027] Figure 7 This is a schematic diagram of the explosion structure of the filter assembly of the utility model;

[0028] Figure 8 It is a schematic diagram of the enlarged structure of point C of the utility model.

[0029] In the figure: 1. preparation box; 2. filter assembly; 21. filter box; 22. connecting frame; 221. positioning groove; 23. cover plate 1; 231. drain port; 232. positioning frame; 24. sealing ring 1; 25. screening filter membrane; 26. cover plate 2; 261. injection port; 262. suction port; 27. sealing ring 2; 28. notch; 29. ​​slot 2; 210. push rod; 211. spring; 212. plug; 3. suction pump; 4. telescopic hose; 5. centrifugal assembly; 51. motor; 52. shaft 1; 53. gear 1; 54. shaft 2; 55. gear 2; 56. connecting shaft; 57. centrifugal barrel; 58. arc sheet; 59. ball; 510. limit groove; 511. slot 1; 512. plug; 513. bump; 514. slide rod. DETAILED DESCRIPTION

[0030] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0031] like Figure 1 , Figure 2 , Figure 6As shown, a device for preparing microbial outer membrane vesicles is provided. The left side of the upper surface of the inner cavity of the preparation box 1 is rotatably connected with a centrifugal component 5, which is convenient for centrifuging the fermentation liquid of the microbial outer membrane vesicles and improving the quality of the finished product. A suction pump 3 is fixedly installed in the middle of the upper surface of the inner cavity of the preparation box 1. The output and input ends of the suction pump 3 are fixedly connected with a telescopic hose 4, which is convenient for extracting the fermentation liquid of the outer membrane vesicles. A filtering component 2 is fixedly installed on the right side of the lower surface of the inner cavity of the preparation box 1 to make the filtering of the outer membrane vesicles more convenient and easy to use. The centrifugal component 5 includes eight protrusions 513, a second rotating shaft 54 ​​and a motor 51. The top of the second rotating shaft 54 ​​is rotatably connected to the left side of the upper surface of the inner cavity of the preparation box 1, and the lower surface of the motor 51 is fixedly installed to the left side of the upper surface of the preparation box 1. The output end of the motor 51 extends to the inner cavity of the preparation box 1 and is fixedly connected with a rotating shaft 1 52. The lower end of the rotating shaft 2 54 A connecting shaft 56 is threadedly connected, and a centrifugal bucket 57 is movably sleeved on the outer surface of the connecting shaft 56. Two arc-shaped pieces 58 are clamped on the upper surface of the centrifugal bucket 57 to prevent the fermentation liquid from leaking when the centrifugal bucket 57 rotates. The filter assembly 2 includes a filter box 21. The lower surface of the filter box 21 is fixedly installed on the right side of the lower surface of the inner cavity of the preparation box 1. The inner cavity of the filter box 21 is fixedly connected with a connecting frame 22. The upper surface of the connecting frame 22 is overlapped with a cover plate 1 23. A screening filter membrane 25 and a cover plate 26 are arranged above the cover plate 1 23. An injection port 261 and a liquid suction port 262 are respectively provided on the left and right sides of the upper surface of the cover plate 26, so that the vesicle fermentation liquid flows laterally under the action of the flow force flowing from the injection port 261 to the liquid suction port 262. A discharge port 231 is provided in the middle of the upper surface of the cover plate 1 23 to facilitate the vesicle fermentation liquid to enter the bottom of the cover plate 1 23.

[0032] The microorganisms are placed in the centrifugal barrel 57, and the arc-shaped piece 58 is stuck on the centrifugal barrel 57 to allow the microorganisms to ferment. When the microorganism fermentation is completed, the motor 51 drives the rotating shaft 2 54, the connecting shaft 56, and the centrifugal barrel 57 to rotate, and the microorganism outer membrane vesicle fermentation liquid is centrifuged. After the centrifugation is allowed to stand for a period of time, the arc-shaped piece 58 is removed, and the left telescopic hose 4 is inserted into the supernatant of the fermentation liquid in the centrifugal barrel 57, and the right telescopic hose 4 is inserted into the injection port 261. The suction pump 3 works to draw the supernatant through the telescopic hose 4 and the injection port 261 into the bottom of the cover plate 26 for filtration, and suction is applied to the liquid suction port 262 to form a pressure difference between the injection port 261 and the liquid suction port 262, so that the vesicle fermentation liquid flows laterally under the flow force from the injection port 261 to the liquid suction port 262, thereby obtaining a sterile filtrate containing microbial outer membrane vesicles, and the microbial outer membrane vesicles can be prepared.

[0033] like Figure 2 As shown, the outer surface of the second rotating shaft 54 ​​is fixedly connected to the second gear 55, and the bottom end of the outer surface of the first rotating shaft 52 is fixedly connected to the first gear 53, and the teeth of the first gear 53 are half of the teeth of the second gear 55, and the left side of the second gear 55 is meshed with the right side of the first gear 53.

[0034] The motor 51 drives the shaft 1 52 and the gear 1 53 to rotate, and the gear 1 53 drives the gear 2 55, the shaft 2 54, the connecting shaft 56, and the centrifugal bucket 57 to rotate intermittently, so that the microbial outer membrane vesicle fermentation liquid can be centrifuged.

[0035] like Figure 2 , Figure 3 , Figure 5 As shown, the inner wall of the axis of the centrifugal barrel 57 is rotatably connected to five balls 59, and the five balls 59 are distributed in three groups in a linear array. The outer surface of the connecting shaft 56 is provided with a limiting groove 510, and the surface of the limiting groove 510 is slidably connected to the outer surface of the ball 59. The lower surface of the centrifugal barrel 57 is fixedly connected to eight slide bars 514, and the eight slide bars 514 are distributed in a circular array. The upper surfaces of the eight protrusions 513 are all fixedly connected to the left side of the lower surface of the inner cavity of the preparation box 1, and the eight protrusions 513 are distributed in a circular array along the axis of the centrifugal barrel 57. The outer surface of the slide bar 514 is slidably connected to the surface of the protrusion 513, and the protrusion 513 has a semi-arc structure.

[0036] The protrusion 513, the slide bar 514, the ball 59, and the limiting groove 510 enable the centrifugal bucket 57 to rotate and move up and down at the same time, so that the fermentation liquid in the centrifugal bucket 57 is vibrated, thereby improving the centrifugal effect of the vesicle fermentation liquid and improving the preparation quality of the finished microbial outer membrane vesicles.

[0037] like Figure 4 As shown, a slot 1 511 is provided on the left side of the outer surface of the connecting shaft 56 near the top, and an insert rod 512 passing through the rotating shaft 2 54 is inserted into the inner wall of the slot 1 511 , and the slot 1 511 is a cylindrical structure.

[0038] The connecting shaft 56 and the second rotating shaft 54 ​​are limited and fixed by the insertion rod 512 to prevent the connecting shaft 56 from loosening when the second rotating shaft 54 ​​drives the connecting shaft 56 to rotate. The connecting shaft 56 is rotated and the insertion rod 512 is removed from the first slot 511 on the connecting shaft 56. The second rotating shaft 54 ​​and the connecting shaft 56 can be disassembled to facilitate cleaning of the sediment in the centrifugal bucket 57.

[0039] like Figure 6 , Figure 7 As shown, the upper surface of the cover plate 1 23 is fixedly connected with a sealing ring 1 24, and the lower surface of the cover plate 26 is fixedly connected with a sealing ring 27. The lower surface of the sealing ring 27 and the upper surface of the sealing ring 1 24 overlap with the upper and lower surfaces of the screening membrane 25 respectively, and the sealing ring 1 24 and the sealing ring 27 are both rectangular structures.

[0040] The fermentation liquid is injected into the bottom of the cover plate 26 through the injection port 261, and suction is applied at the suction port 262 to form a pressure difference between the injection port 261 and the suction port 262, so that the vesicle fermentation liquid flows laterally under the flow force from the injection port 261 to the suction port 262. The vesicle fermentation liquid on the surface of the screening filter membrane 25 is affected by both the flow force flowing toward the discharge port 231 and the flow force flowing toward the suction port 262, so that the vesicle fermentation liquid with a diameter smaller than the filter hole of the screening filter membrane 25 can pass through the filter hole and enter the bottom of the cover plate 1 23 through the discharge port 231, while the vesicle fermentation liquid with a diameter larger than the filter hole cannot pass through the filter hole. The lateral flow force will drive the vesicle fermentation liquid with a larger diameter to flow laterally to prevent the filter hole from being blocked, thereby reducing the probability of the filter hole being blocked and the vesicle being squeezed and destroyed during vesicle screening, thereby improving the screening efficiency.

[0041] like Figure 7 , Figure 8 As shown, a slot 29 is provided in the middle of the left and right sides of the cover plate 26 and the cover plate 1 23, and a notch 28 is provided on the left and right sides of the inner cavity of the filter box 21. A spring 211 is fixedly connected to the side of the inner cavity of the notch 28 away from the cover plate 26, and the other end of the spring 211 is fixedly connected to an insert block 212, and the outer surface of the insert block 212 is plugged into the inner wall of the slot 29. Push rods 210 are movably connected to the inner walls of the left and right sides of the filter box 21, and one end of the push rod 210 is fixedly connected to the side of the slot 29 away from the cover plate 26. The slot 29 and the insert block 212 are conical structures on the side close to the cover plate 26. A positioning frame 232 is fixedly connected to the lower surface of the cover plate 1 23, and a positioning groove 221 is provided on the upper surface of the connecting frame 22. The inner wall of the positioning groove 221 is plugged into the outer surface of the positioning frame 232, and the positioning frame 232 and the positioning groove 221 are both rectangular structures.

[0042] By moving the push rod 210, the spring 211, and the plug block 212, the cover plate 26 and the cover plate 1 23 can be disassembled, so that the screening filter membrane 25 can be replaced. By setting the rectangular sealing ring 27 and the sealing ring 1 24, the vesicle fermentation liquid can be prevented from leaking during filtration.

[0043] The working principle of the present invention is as follows: microorganisms are placed in a centrifugal barrel 57, and an arc-shaped piece 58 is clamped on the centrifugal barrel 57 to allow the microorganisms to ferment. When the fermentation of the microorganisms is completed, the motor 51 drives the rotating shaft 1 52 and the gear 1 53 to rotate, and the gear 1 53 drives the gear 2 55, the rotating shaft 2 54, the connecting shaft 56, and the centrifugal barrel 57 to rotate intermittently. The protrusion 513, the slide bar 514, the ball 59, and the limit groove 510 enable the centrifugal barrel 57 to rotate and move up and down at the same time, so that the fermentation liquid in the centrifugal barrel 57 is vibrated, thereby improving the centrifugal effect of the vesicle fermentation liquid, thereby improving the preparation quality of the finished microbial outer membrane vesicles. The centrifuge is allowed to stand for a period of time, the arc-shaped piece 58 is removed, the telescopic hose 4 on the left side is inserted into the supernatant of the fermentation liquid in the centrifugal barrel 57, and the telescopic hose 4 on the right side is inserted into the injection port 261. The suction pump 3 works to pump the fermentation liquid in the centrifugal barrel 57 through the telescopic hose 4. The liquid is injected into the bottom of the cover plate 26 through the injection port 261, and suction is applied to the liquid suction port 262 to form a pressure difference between the injection port 261 and the liquid suction port 262, so that the vesicle fermentation liquid flows laterally under the flow force from the injection port 261 to the liquid suction port 262. The vesicle fermentation liquid on the surface of the screening filter membrane 25 is affected by both the flow force flowing toward the discharge port 231 and the flow force flowing toward the liquid suction port 262, so that the vesicle fermentation liquid with a diameter smaller than the filter hole of the screening filter membrane 25 can pass through the filter hole and enter the bottom of the cover plate 23 through the discharge port 231. The vesicle fermentation liquid with a diameter larger than the filter hole cannot pass through the filter hole. The lateral flow force will drive the vesicle fermentation liquid with a larger diameter to flow laterally to prevent the filter hole from being blocked, thereby reducing the probability of the filter hole being blocked and the vesicle being squeezed and destroyed during vesicle screening, thereby improving the screening efficiency, so that a sterile filtrate containing microbial outer membrane vesicles can be obtained, and the microbial outer membrane vesicles can be prepared.

[0044] The above generally describes the present invention in detail, but it is obvious to a person skilled in the art that some modifications or improvements can be made to the present invention. Therefore, modifications or improvements that do not deviate from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A microbial outer membrane vesicle preparation device, comprising a preparation box (1), characterized in that: A centrifugal assembly (5) is rotatably connected to the left side of the upper surface of the inner cavity of the preparation box (1), a suction pump (3) is fixedly installed in the middle of the upper surface of the inner cavity of the preparation box (1), and the output end and input end of the suction pump (3) are both fixedly connected to a telescopic hose (4), and a filter assembly (2) is fixedly installed on the right side of the lower surface of the inner cavity of the preparation box (1); The centrifugal assembly (5) comprises eight protrusions (513), a second rotating shaft (54) and a motor (51); the top end of the second rotating shaft (54) is rotatably connected to the left side of the upper surface of the inner cavity of the preparation box (1); the lower surface of the motor (51) is fixedly installed to the left side of the upper surface of the preparation box (1); the output end of the motor (51) extends to the inner cavity of the preparation box (1) and is fixedly connected to the first rotating shaft (52); the lower end of the second rotating shaft (54) is threadedly connected to a connecting shaft (56); the outer surface of the connecting shaft (56) is movably sleeved with a centrifugal bucket (57); and the upper surface of the centrifugal bucket (57) is clamped with two arc-shaped sheets (58); The filter assembly (2) comprises a filter box (21), the lower surface of the filter box (21) being fixedly mounted to the right side of the lower surface of the inner cavity of the preparation box (1), the inner cavity of the filter box (21) being fixedly connected to a connection frame (22), the upper surface of the connection frame (22) being overlapped with a cover plate 1 (23), a screening filter membrane (25) and a cover plate 2 (26) being arranged above the cover plate 1 (23), an injection port (261) and a liquid suction port (262) being respectively provided on the left and right sides of the upper surface of the cover plate 2 (26), and a liquid discharge port (231) being provided in the middle of the upper surface of the cover plate 1 (23).

2. A microbial outer membrane vesicle preparation device according to claim 1, characterized in that: The outer surface of the second rotating shaft (54) is fixedly connected to a second gear (55), the bottom end of the outer surface of the first rotating shaft (52) is fixedly connected to a first gear (53), and the teeth of the first gear (53) are half of those of the second gear (55), the left side of the second gear (55) is meshed with the right side of the first gear (53), and the upper surface of the centrifugal bucket (57) is clamped with two arc-shaped pieces (58).

3. A microbial outer membrane vesicle preparation device according to claim 1, characterized in that: A slot 1 (511) is provided on the left side of the outer surface of the connecting shaft (56) near the top, and an insertion rod (512) passing through the second rotating shaft (54) is inserted into the inner wall of the slot 1 (511), and the slot 1 (511) is a cylindrical structure.

4. A microbial outer membrane vesicle preparation device according to claim 1, characterized in that: The inner wall of the axis of the centrifugal barrel (57) is rotatably connected to five balls (59), and the five balls (59) are distributed in three groups in a linear array. The outer surface of the connecting shaft (56) is provided with a limiting groove (510), and the surface of the limiting groove (510) is slidably connected to the outer surface of the balls (59).

5. A device for preparing microbial outer membrane vesicles according to claim 1, characterized in that: The lower surface of the centrifugal bucket (57) is fixedly connected to eight sliding bars (514), and the eight sliding bars (514) are distributed in a circular array. The upper surfaces of the eight protrusions (513) are fixedly connected to the left side of the lower surface of the inner cavity of the preparation box (1), and the eight protrusions (513) are distributed in a circular array along the axis of the centrifugal bucket (57). The outer surface of the sliding bar (514) is slidably connected to the surface of the protrusion (513), and the protrusion (513) has a semi-arc structure.

6. A device for preparing microbial outer membrane vesicles according to claim 1, characterized in that: The upper surface of the cover plate 1 (23) is fixedly connected to a sealing ring 1 (24), and the lower surface of the cover plate 2 (26) is fixedly connected to a sealing ring 2 (27). The lower surface of the sealing ring 2 (27) and the upper surface of the sealing ring 1 (24) overlap with the upper and lower surfaces of the screening filter membrane (25) respectively. The sealing ring 1 (24) and the sealing ring 2 (27) are both rectangular structures.

7. A device for preparing microbial outer membrane vesicles according to claim 1, characterized in that: A second slot (29) is provided in the middle of the left and right sides of the second cover plate (26) and the first cover plate (23); a notch (28) is provided on the left and right side surfaces of the inner cavity of the filter box (21); a spring (211) is fixedly connected to the side of the inner cavity of the notch (28) away from the second cover plate (26); an insert block (212) is fixedly connected to the other end of the spring (211); an outer surface of the insert block (212) is plugged into the inner wall of the second slot (29); push rods (210) are movably connected to the inner walls of the left and right sides of the filter box (21); one end of the push rod (210) is fixedly connected to the side of the second slot (29) away from the second cover plate (26); and the second slot (29) and the insert block (212) are conical structures on the side close to the second cover plate (26).

8. A device for preparing microbial outer membrane vesicles according to claim 1, characterized in that: A positioning frame (232) is fixedly connected to the lower surface of the cover plate 1 (23); a positioning groove (221) is provided on the upper surface of the connection frame (22); an inner wall of the positioning groove (221) is plugged into an outer surface of the positioning frame (232); and both the positioning frame (232) and the positioning groove (221) are rectangular structures.

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