Carbon dioxide light-operated bioreactor adapted to growth of photosynthetic bacteria

Abstract

The utility model discloses a carbon dioxide light-operated bioreactor adaptive to the growth of photosynthetic bacteria, which comprises a reactor tank body, an illumination device, a detection device, a stirring device and a carbon dioxide gas circulation system, the tank body is provided with a feed port, an inoculation port, an air inlet pipe, the illumination device and a stirring device, and the detection device comprises a sensor arranged on the tank body and a control module communicated with the sensor. According to the utility model, the carbon dioxide circulating system is used as a carbon source supply system of photosynthetic bacteria, so that the normal growth of the photosynthetic bacteria is ensured, and meanwhile, the efficiency problem of carbon reduction and carbon sequestration is solved; the electromagnetic valve and the air pump are intelligently controlled through the CO2 concentration regulator, efficient cyclic utilization of carbon dioxide is achieved, meanwhile, supply of a carbon source in a culture medium is omitted, and the problem of later-stage material supplementing is avoided. Operation is simple, and large-scale verification and use in laboratories and factories are facilitated.

Description

Technical Field

[0001] The utility model belongs to the technical field of bioreactors, and in particular relates to a carbon dioxide light-controlled bioreactor suitable for the growth of photosynthetic bacteria. Background Art

[0002] In recent years, modern biotechnology represented by genetic engineering, enzyme engineering, and fermentation engineering has developed rapidly and has increasingly influenced and changed people's production and lifestyle. Fermentation tanks are important bioengineering equipment used to cultivate and ferment various bacteria. Among them, photosynthetic bacteria are a type of microorganism that uses light as energy and can use organic matter, sulfide, ammonia, etc. in nature as hydrogen and carbon sources for photosynthesis under anaerobic light or aerobic dark conditions. They are widely used in the fields of water purification, feed, and disease prevention in the aquaculture industry.

[0003] There are few types of bioreactor structures suitable for photosynthetic bacteria growth in the prior art, but in the field of carbon dioxide as a carbon source for cyclic fermentation, there is still a blank in China, and the problem that carbon dioxide is not easily soluble in water has not been properly solved. The gas cycle changes in peak value, which is discontinuous and unstable, and it is difficult to circulate and use efficiently. In terms of light source, due to the uneven heat of the external light source, it is difficult to irradiate for fermentation culture, and it has been gradually replaced by internal light source, but its structure is single, and the heat of the single interlayer light source is relatively concentrated, which is difficult to control, resulting in low light energy conversion rate, which has a great impact on the safety of fermentation culture and needs to be improved urgently. Utility Model Content

[0004] The technical problem to be solved by the utility model is that it is difficult for existing bioreactors to control carbon dioxide as a stable carbon source for the growth of photosynthetic bacteria, and the structure of the light source is single and difficult to control separately. To this end, the utility model provides a carbon dioxide light-controlled bioreactor adapted for the growth of photosynthetic bacteria, the bioreactor has the characteristics of reasonable, high-performance and environmentally friendly carbon dioxide supply settings, and at the same time monitors the photosynthetic bacteria growth environment by detecting the temperature, pH, light intensity, carbon dioxide concentration, and carbon dioxide solubility, so as to effectively grasp the fermentation situation and fermentation environment in real time, and the fermentation process is safe, environmentally friendly, energy-saving and efficient.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A carbon dioxide light-controlled bioreactor suitable for the growth of photosynthetic bacteria comprises a tank body, an illumination device, a detection device, a stirring device and a carbon dioxide gas circulation system. The tank body is connected to the carbon dioxide gas circulation system. The tank body is provided with a feed port, an inoculation port, an air inlet pipe, the illumination device and the stirring device. The detection device comprises a sensor arranged on the tank body and a control module connected to the sensor.

[0007] Furthermore, the tank body is provided with a carbon dioxide electrode and a temperature and photometry electrode.

[0008] Furthermore, the sensor includes a pH sensor, a temperature sensor, a photometric sensor and a CO2 concentration sensor, and the pH sensor, temperature sensor, photometric sensor and CO2 concentration sensor are respectively connected to the pH regulator, temperature regulator, light regulator and CO2 concentration regulator in the control module.

[0009] Furthermore, the carbon dioxide gas circulation system includes: a gas storage device, an air intake pump, an air outlet pump, an air intake solenoid valve, and an air outlet solenoid valve. The air intake solenoid valve is connected to the air intake pump, the air outlet solenoid valve is connected to the air outlet pump, and the gas storage device is connected to the air intake pump and the air outlet pump, respectively.

[0010] Furthermore, the control module controls the start and stop of the air intake solenoid valve and the air outlet solenoid valve through output signals.

[0011] Furthermore, the connection and disconnection time of the air intake solenoid valve is adjusted and determined by the control module according to the carbon dioxide concentration in the reactor.

[0012] Furthermore, the lighting device includes a glass tube, an LED light group, a sealing ring, and a built-in fixing plate.

[0013] Furthermore, a plurality of LED light groups are arranged in the glass tube, and the LED light groups are fixed on a built-in fixing plate.

[0014] Furthermore, a condensation hood is arranged outside the tank body.

[0015] Furthermore, the gas storage device is a special large-capacity air bag.

[0016] Compared with the prior art, the advantages and beneficial effects of the utility model are:

[0017] 1. In the carbon dioxide gas circulation system provided in the utility model, the solenoid valve and the air pump are intelligently controlled by the CO2 concentration regulator to adjust the CO2 flow rate and flow rate introduced into the reactor, thereby realizing efficient recycling of carbon dioxide, and at the same time eliminating the carbon source supply in the culture medium, avoiding the problem of late feeding, and improving the working efficiency of the system.

[0018] 2. The utility model uses a reasonable CO2 concentration gradient adjustment system to make the CO2 concentration provided by the system meet the carbon source demand of photosynthetic bacteria light culture, and the carbon dioxide concentration can be automatically adjusted through the carbon dioxide electrode and the carbon dioxide concentration adjustment device. The staggered inlet and outlet gas control with a certain pressure is used to facilitate efficient and stable gas circulation, avoid the problem of reciprocating intermittent inlet and outlet gas, and ensure the stable increase and decrease of CO2 gradient. The photosynthetic bacteria are always in a growth environment suitable for CO2 supply, which improves the fermentation efficiency and quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some implementation examples of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0020] Figure 1 This is a system diagram of the carbon dioxide light-controlled bioreactor of the utility model;

[0021] Figure 2 The three-side structural view of the carbon dioxide light-controlled bioreactor tank of the utility model;

[0022] Figure 3 This is an axonometric view of the tank of the carbon dioxide light-controlled bioreactor of the utility model;

[0023] Figure 4 This is a schematic diagram of the structure of the lighting device of the utility model;

[0024] Among them, 1. feed port; 2. inoculation port; 3. lighting device; 4. carbon dioxide electrode; 5. temperature and photometry electrode; 6. stirring device; 7. air inlet pipe; 8. tank body; 9. condensation cover;

[0025] 3-1, glass tube; 3-2, LED light assembly; 3-3, sealing ring; 3-4, built-in fixing plate;

[0026] 10-1, gas storage device; 10-2, air intake pump; 10-3, air outlet pump; 10-4, air intake solenoid valve; 10-5, air outlet solenoid valve. DETAILED DESCRIPTION

[0027] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings. The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0028] Example 1

[0029] like Figure 1 and Figure 2 As shown, the carbon dioxide light-controlled bioreactor adapted for photosynthetic bacteria growth provided by the utility model comprises a tank body 8, an illumination device 3, a detection device, a stirring device 6 and a carbon dioxide gas circulation system. The tank body 8 is connected to the carbon dioxide gas circulation system, and the tank body 8 is provided with a feed port 1, an inoculation port 2, an illumination device 3, a carbon dioxide electrode 4, a temperature and photometric electrode 5, a stirring device 6 and an air inlet pipe 7. The detection device comprises a sensor arranged on the tank body and a control module connected to the sensor.

[0030] The sensor includes a pH sensor, a temperature sensor, a photometric sensor and a CO2 concentration sensor, and the pH sensor, the temperature sensor, the photometric sensor and the CO2 concentration sensor are respectively connected to the pH regulator, the temperature regulator, the light regulator and the CO2 concentration regulator in the control module. The carbon dioxide electrode 4 is connected to the CO2 concentration regulator, and the temperature and photometric electrode 5 is connected to the temperature regulator and the light regulator.

[0031] The stirring device 6 is fixedly arranged on the upper side of the tank body 8. The stirring device 6 can make the fermentation liquid flow continuously and evenly, which is beneficial for the photosynthetic bacteria in each part to obtain sufficient light intensity and is beneficial to the fermentation of the photosynthetic bacteria.

[0032] The lighting device includes a glass tube, an LED light group, a sealing ring, and a built-in fixing plate (built-in PCB board). Figure 4 As shown, in each glass tube, 8 sets of LED light groups 3-2 are arranged side by side on a built-in PCB board 3-4, wherein each set of LED light groups 3-2 is composed of 6 LED lights, and the arrangement of 8 sets of light groups 3-2 in each lamp tube can provide sufficient light intensity. The eight sets of LED light groups light up at intervals as needed, and the light intensity is divided into level one, level two, level three, and level four, which are controlled by the corresponding two, four, six, and eight sets of LED light groups respectively.

[0033] The carbon dioxide circulation system includes a gas storage device (special large-capacity air bag) 10-1, an air intake pump 10-2, an air intake solenoid valve 10-4, an air outlet pump 10-3, and an air outlet solenoid valve 10-5. The air intake solenoid valve 10-4 is connected to the air intake pump 10-2, and the air outlet solenoid valve 10-5 is connected to the air outlet pump 10-3. The gas storage device (special large-capacity air bag) 10-1 is connected to the air intake pump 10-2 and the air outlet pump 10-3 through pipelines. Compared with gas tanks, large-capacity air bags can be easily moved and placed.

[0034] The CO2 concentration regulator in the control module can adjust the CO2 flow rate and flow rate change in the reactor in a gradient manner, and make real-time automatic adjustments after the monitoring feedback of the carbon dioxide electrode. It controls the air pump and the air inlet and outlet solenoid valves to open on demand for a fixed time, and adopts a certain pressure offset inlet and outlet control to ensure that the air inlet and outlet pumps work normally. It is conducive to efficient and stable gas circulation, avoids the problem of reciprocating intermittent air inlet and outlet, and ensures the stable increase and decrease of the CO2 gradient.

[0035] The air inlet solenoid valve and the air outlet solenoid valve are used as control elements and are controlled by the control module. The connection and disconnection time are adjusted and determined by the control module according to the carbon dioxide concentration in the reactor. When the gas circulation system is ventilated, the control module controls the air inlet pump 10-2 to draw gas from the gas tank. At this time, the system automatically opens the air inlet solenoid valve 10-4. After the set time of the air outlet solenoid valve 10-5 is over, the system opens the air outlet solenoid valve 10-5 and starts the air outlet pump 10-3 to pump gas into the gas storage device (special large-capacity air bag) 10-1. After the same time as ventilation, the second round of periodic reciprocating gas circulation with the same process is started to ensure sufficient supply of carbon dioxide in the reactor and the normal growth of photosynthetic bacteria.

[0036] According to the needs of the growth and synthesis of photosynthetic bacteria, the CO2 concentration and the solubility parameters in the culture medium are set in the control module, and the carbon dioxide concentration in the tank body 8 is monitored by the carbon dioxide electrode 5 and transmitted to the control module for adjustment. When the concentration is insufficient, the control module transmits a control signal to the carbon dioxide regulator to adjust the carbon dioxide flow rate and flow size, and by starting the solenoid valve and the inlet and outlet air pumps, the operating power of the air pump is adjusted, and the carbon dioxide flow rate is controlled to increase as required, ensuring that the carbon dioxide dissolved in the water in the tank can ensure the normal growth of the strain; when the carbon dioxide concentration is too high, the external control system transmits a control signal to the microcontroller to adjust the carbon dioxide concentration, and by opening the outlet solenoid valve and the outlet air pump, the carbon dioxide is pumped into the gas storage device, so that the carbon dioxide concentration in the tank body is weakened.

[0037] A condensation hood 9 is also provided outside the tank body. The setting of the condensation hood can control the temperature inside the fermentation tank within an appropriate range, avoiding adverse effects of excessive temperature on the growth of microorganisms and the fermentation process, ensuring that the temperature and environmental conditions of the fermentation process are effectively controlled, thereby ensuring the quality and yield of the fermentation product.

[0038] The above-mentioned embodiments only represent the implementation methods of the present utility model, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the present utility model. It should be pointed out that for those skilled in the art, several variations and improvements can be made without departing from the concept of the present utility model, and these all belong to the protection scope of the present utility model.

Claims

1. A carbon dioxide light-controlled bioreactor adapted for the growth of photosynthetic bacteria, characterized in that: It includes a tank body, a lighting device, a detection device, a stirring device and a carbon dioxide gas circulation system. The tank body is connected to the carbon dioxide gas circulation system. The tank body is provided with a feed port, an inoculation port, an air inlet pipe, the lighting device and the stirring device. The detection device includes a sensor arranged on the tank body and a control module connected to the sensor.

2. The carbon dioxide light-controlled bioreactor according to claim 1, characterized in that: The tank body is provided with a carbon dioxide electrode and a temperature and photometric electrode.

3. The carbon dioxide light-controlled bioreactor according to claim 1, characterized in that: The sensors include a pH sensor, a temperature sensor, a photometric sensor and a CO2 concentration sensor, and the pH sensor, the temperature sensor, the photometric sensor and the CO2 concentration sensor are respectively connected to a pH regulator, a temperature regulator, a light regulator and a CO2 concentration regulator in a control module.

4. The carbon dioxide light-controlled bioreactor according to claim 1, characterized in that: The carbon dioxide gas circulation system comprises: a gas storage device, an air intake pump, an air outlet pump, an air intake solenoid valve, and an air outlet solenoid valve. The air intake solenoid valve is connected to the air intake pump, the air outlet solenoid valve is connected to the air outlet pump, and the gas storage device is connected to the air intake pump and the air outlet pump, respectively.

5. The carbon dioxide light-controlled bioreactor according to claim 1, characterized in that: The control module controls the start and stop of the air inlet solenoid valve and the air outlet solenoid valve through output signals.

6. The carbon dioxide light-controlled bioreactor according to claim 5, characterized in that: The connection and disconnection time of the air intake solenoid valve is adjusted and determined by the control module according to the carbon dioxide concentration in the reactor.

7. The carbon dioxide light-controlled bioreactor according to claim 1, characterized in that: The lighting device comprises a glass tube, an LED lamp group, a sealing ring, and a built-in fixing plate.

8. The carbon dioxide light-controlled bioreactor according to claim 7, characterized in that: A plurality of LED light groups are arranged in the glass tube, and the LED light groups are fixed on a built-in fixing plate.

9. The carbon dioxide light-controlled bioreactor according to claim 1, characterized in that: A condensation hood is arranged outside the tank body.

10. The carbon dioxide light-controlled bioreactor according to claim 4, characterized in that: The gas storage device is a special large-capacity air bag.

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