AAV purification device

Abstract

The utility model relates to the technical field of purification, in particular to an AAV purification device which comprises a purification machine, a centrifugal assembly and a liquid collector, the centrifugal assembly is connected to the interior of the purification machine, a plurality of sets of supporting assemblies are connected to the centrifugal assembly, the liquid collector is connected among the multiple sets of supporting assemblies, the lower end of the centrifugal assembly is connected with a connecting column, and the connecting column is connected with the liquid collector. A centrifugal motor is connected to the lower end of the connecting column, a control panel is arranged on the front face of the purifying machine, a pressing groove is formed in the upper end of the purifying machine, a bearing is arranged in the middle of the lower end of the pressing groove, and the centrifugal assembly is composed of a rotating disc, an upper rack, a connecting sleeve column, a pull groove, a pull ring, a turning cover and a limiting groove. The liquid collector is composed of a liquid collecting box, an inserting groove, an inserting cover and a centrifugal tube, the supporting assembly is composed of a supporting rod, a telescopic rod, a baffle, a reset spring and a connecting base, an upper bevel gear is arranged at the lower end of a connecting column, and one end of the centrifugal motor is connected with a lower bevel gear.

Description

Technical Field

[0001] The utility model relates to the technical field of purification, in particular to an AAV purification device. Background Art

[0002] Adeno-Associated Virus (AAV) is an important tool in the field of gene therapy. Its successful application not only depends on the virus's efficient transmission ability, but also requires precise separation and purification during the preparation process to ensure that pure and effective AAV vectors are obtained.

[0003] Therefore, it is necessary to design an AAV purification device to solve the problems in the above-mentioned background technology. Utility Model Content

[0004] The utility model aims to solve the problems in the prior art and proposes an AAV purification device.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] An AAV purification device comprises a purification machine, a centrifugal assembly and a liquid collector, wherein the purification machine is internally connected with the centrifugal assembly, a plurality of groups of support assemblies are connected to the centrifugal assembly, a liquid collector is connected between the plurality of groups of support assemblies, a connecting column is connected to the lower end of the centrifugal assembly, and a centrifugal motor is connected to the lower end of the connecting column;

[0007] A maintenance plate is connected to one side of the purifier, an adjustment foot is connected to the lower end of the purifier, a control panel is provided on the front of the purifier, a buckle groove is provided at the upper end of the purifier near the front, a pressure groove is provided at the upper end of the purifier, and flip doors are connected to both sides of the pressure groove near the back, an electromagnetic lock is provided at the lower end of the flip door near both sides of the front, a limit column is provided at the lower end of the flip door near both sides of the back, a pull plate is provided in the middle of the front of the flip door, and a bearing is provided in the middle of the lower end of the pressure groove;

[0008] The centrifugal assembly is composed of a turntable, an upper frame, a connecting sleeve, a draw groove, a pull ring, a flap and a limit groove, and a connecting sleeve is provided in the middle of the upper end of the turntable, an upper frame is provided on the upper part of the connecting sleeve, a pull ring is provided on the upper end of the connecting sleeve, a plurality of limit grooves are provided on the outer side of the upper frame, a plurality of flaps are provided on the upper end of the upper frame, and a plurality of draw grooves are provided on the upper part of the outer part of the turntable;

[0009] The liquid collector is composed of a liquid collecting box, a plug-in slot, a plug-in cover and a centrifuge tube, and the upper end of the liquid collecting box is connected to the plug-in cover, the plug-in slot is provided on the outside of the plug-in cover, and the upper end of the plug-in cover is connected to multiple groups of centrifuge tubes;

[0010] The support assembly is composed of a support rod, a telescopic rod, a baffle, a return spring and a connecting seat, and the connecting seat is connected to one side of the telescopic rod, the telescopic rod is provided with a return spring outside, the telescopic rod is provided with a baffle on the side away from the connecting seat, and the baffle is provided with a support rod on the side away from the telescopic rod;

[0011] An upper bevel gear is provided at the lower end of the connecting column;

[0012] One end of the centrifugal motor is connected with a lower bevel gear.

[0013] As a preferred solution of the utility model, the purifier and the inspection plate are connected by bolts, and a heat dissipation grid is provided on the inspection plate.

[0014] As a preferred solution of the utility model, the flip-up door is connected to the purification machine through an electromagnetic lock, the limit column and the purification machine are connected through plug-in connection, and the pull plate and the buckle groove are connected through sleeve connection.

[0015] As a preferred solution of the utility model, the connecting column is connected to the purifier through a bearing, the upper bevel gear and the lower bevel gear are meshingly connected, the lower bevel gear and the centrifugal motor are connected through a coupling, the lower bevel gear and the purifier are connected through a bearing, and the centrifugal motor and the purifier are connected through a hoop.

[0016] As a preferred solution of the utility model, the connecting column and the turntable are screwed together by threads, the connecting seat and the purification machine are connected by bolts, the support rod and the purification machine are connected by sleeve connection, and the support rod and the socket slot are connected by plug connection.

[0017] As a preferred solution of the utility model, the plug-in cover and the liquid collecting box are screwed together by threads, and the centrifuge tube and the plug-in cover are plugged together.

[0018] In summary, the technical effects and advantages of the utility model are as follows: the AAV purification device can separate large particles such as cell fragments, proteins, DNA, etc. through centrifugation during AAV purification, while retaining the AAV vector. The purity of AAV can be gradually improved through multiple centrifugation and centrifugation steps at different speeds. Before centrifugation, multiple groups of centrifuge tubes are plugged into the plug-in cover, and then the liquid collector is sleeved on the support rod. When centrifugation is required, the upper flip door is pressed down and locked by the electromagnetic lock, and then the lower bevel gear, the upper bevel gear and the centrifugal assembly are driven by the centrifugal motor to rotate centrifugally. During centrifugation, the liquid collector can rotate along the support rod, which is more adaptable to centrifugal force and easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is the overall structure diagram of the utility model;

[0020] Figure 2 This is a combined structural diagram of the centrifugal assembly and the liquid collector of the utility model;

[0021] Figure 3 This is a structural diagram of the centrifugal assembly of the utility model;

[0022] Figure 4 This is a structural diagram of the liquid collector of the utility model;

[0023] Figure 5 This is a structural diagram of the support assembly of the utility model;

[0024] Figure 6 This is a structural diagram of the centrifugal motor of the present utility model.

[0025] In the figure: 1. Purifier; 101. Inspection panel; 102. Adjustment foot; 103. Control panel; 104. Buckle slot; 105. Limiting column; 106. Upward flip door; 107. Electromagnetic lock; 108. Pull plate; 109. Pressing groove; 110. Bearing; 2. Centrifugal assembly; 201. Turntable; 202. Upper frame; 203. Connecting sleeve column; 204. Pulling groove; 205. Pull ring; 206. Flip cover; 207. Limiting groove; 3. Liquid collector; 301. Liquid collecting box; 302. Plug-in slot; 303. Plug-in cover; 304. Centrifuge tube; 4. Support assembly; 401. Support rod; 402. Telescopic rod; 403. Baffle; 404. Reset spring; 405. Connecting seat; 5. Connecting column; 501. Upper bevel gear; 6. Centrifugal motor; 601. Lower bevel gear. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0027] Reference Figure 1-Figure 6, an AAV purification device comprises a purification machine 1, a centrifugal assembly 2 and a liquid collector 3, the purification machine 1 is internally connected with the centrifugal assembly 2, a plurality of support assemblies 4 are connected to the centrifugal assembly 2, the plurality of support assemblies 4 are connected with the liquid collector 3, the lower end of the centrifugal assembly 2 is connected with a connecting column 5, and the lower end of the connecting column 5 is connected with a centrifugal motor 6; an inspection plate 101 is connected to one side of the purification machine 1, an adjusting foot 102 is connected to the lower end of the purification machine 1, a control panel 103 is provided on the front of the purification machine 1, a buckle groove 104 is provided on the upper end of the purification machine 1 near the front, and the purification machine 1 A pressing groove 109 is provided at the upper end, and an upper flip door 106 is connected to both sides of the pressing groove 109 near the back, and an electromagnetic lock 107 is provided at both sides of the lower end of the upper flip door 106 near the front, and a limit column 105 is provided at both sides of the lower end of the upper flip door 106 near the back, and a pull plate 108 is provided in the middle of the front of the upper flip door 106, and a bearing 110 is provided in the middle of the lower end of the pressing groove 109; the centrifugal assembly 2 is composed of a turntable 201, an upper frame 202, a connecting sleeve 203, a pull groove 204, a pull ring 205, a flip cover 206 and a limit groove 207, and the middle of the upper end of the turntable 201 is provided with a pull plate 108, and a bearing 110 is provided in the middle of the lower end of the pressing groove 109; A connecting sleeve 203 is provided at the middle, an upper frame 202 is provided on the upper part of the connecting sleeve 203, a pull ring 205 is provided on the upper end of the connecting sleeve 203, a plurality of limit grooves 207 are provided on the outer side of the upper frame 202, a plurality of flip covers 206 are provided on the upper end of the upper frame 202, and a plurality of pull grooves 204 are provided on the upper part of the outer part of the turntable 201; the liquid collector 3 is composed of a liquid collecting box 301, a plug-in slot 302, a plug-in cover 303 and a centrifuge tube 304, and the upper end of the liquid collecting box 301 is connected to a plug-in cover 303, a plug-in slot 302 is provided on the outer side of the plug-in cover 303, and a plug-in cover 304 is provided on the outer side of the plug-in cover 303. A plurality of centrifuge tubes 304 are connected to the upper end of 303; the support assembly 4 is composed of a support rod 401, a telescopic rod 402, a baffle 403, a return spring 404 and a connecting seat 405, and the connecting seat 405 is connected to one side of the telescopic rod 402, a return spring 404 is arranged outside the telescopic rod 402, a baffle 403 is arranged on the side of the telescopic rod 402 away from the connecting seat 405, and a support rod 401 is arranged on the side of the baffle 403 away from the telescopic rod 402; an upper bevel gear 501 is arranged at the lower end of the connecting column 5; a lower bevel gear 601 is connected to one end of the centrifugal motor 6.

[0028] Reference Figure 1 and Figure 6The purifier 1 and the inspection plate 101 are connected by bolts, the inspection plate 101 is provided with a heat dissipation grid, the flip door 106 is connected to the purifier 1 by an electromagnetic lock 107, the limit column 105 and the purifier 1 are connected by plugging, the pull plate 108 and the buckle groove 104 are connected by sleeve connection, the connecting column 5 is connected to the purifier 1 by a bearing 110, the upper bevel gear 501 and the lower bevel gear 601 are meshed, the lower bevel gear 601 and the centrifugal motor 6 are connected by a coupling, the lower bevel gear 601 and the purifier 1 are connected by a bearing, and the centrifugal motor 6 and the purifier 1 are connected by a hoop. When centrifugation is required, the flip door 106 is pressed down and locked by the electromagnetic lock 107, and then the lower bevel gear 601, the upper bevel gear 501 and the centrifugal assembly 2 are driven by the centrifugal motor 6 to rotate centrifugally, which is easy to operate.

[0029] Reference Figure 1-Figure 5 Adeno-Associated Virus (AAV) is an important tool in the field of gene therapy. Its successful application not only depends on the efficient transmission ability of the virus, but also requires precise separation and purification during the preparation process to ensure that a pure and effective AAV vector is obtained. The connecting column 5 and the turntable 201 are screwed together, the connecting seat 405 and the purifier 1 are bolted together, the support rod 401 and the purifier 1 are connected by sleeve connection, the support rod 401 and the plug-in slot 302 are plug-in connected, and the plug-in cover 303 and the liquid collection box 30 1 is screwed together by threads, and the centrifuge tube 304 and the plug-in cover 303 are plugged together. During AAV purification, large particles such as cell debris, protein, and DNA can be separated by centrifugation, while retaining the AAV vector. The purity of AAV can be gradually improved through multiple centrifugation steps and centrifugation steps at different speeds. Before centrifugation, multiple groups of centrifuge tubes 304 are plugged into the plug-in cover 303, and then the liquid collector 3 is sleeved on the support rod 401. During centrifugation, the liquid collector 3 can rotate along the support rod 401 to better adapt to the centrifugal force.

[0030] Working principle: AAV purification device, the purification machine 1 and the inspection plate 101 are connected by bolts, the inspection plate 101 is provided with a heat dissipation grid, the upper flip door 106 is connected to the purification machine 1 by an electromagnetic lock 107, the limit column 105 and the purification machine 1 are connected by plugging, the pull plate 108 and the buckle groove 104 are connected by sleeve connection, the connecting column 5 is connected to the purification machine 1 by a bearing 110, the upper bevel gear 501 and the lower bevel gear 601 are meshed, the lower bevel gear 601 and the centrifugal motor 6 are connected by a coupling, the lower bevel gear 601 and the purification machine 1 are connected by a bearing, and the centrifugal motor 6 and the purification machine 1 are connected by a hoop. When centrifugation is required, the upper flip door 106 is pressed down and locked by the electromagnetic lock 107, and then the lower bevel gear 601, the upper bevel gear 501 and the centrifugal assembly 2 are driven by the centrifugal motor 6 to perform centrifugal rotation. The invention is easy to operate, the connecting column 5 and the turntable 201 are screwed together, the connecting seat 405 and the purification machine 1 are bolted together, the support rod 401 and the purification machine 1 are sleeved together, the support rod 401 and the plug-in slot 302 are plugged together, the plug-in cover 303 and the liquid collection box 301 are screwed together, and the centrifuge tube 304 and the plug-in cover 303 are plugged together. During AAV purification, large particles such as cell debris, protein, and DNA can be separated by centrifugation, while retaining the AAV vector. The purity of AAV can be gradually improved through multiple centrifugation and centrifugation steps at different speeds. Before centrifugation, multiple groups of centrifuge tubes 304 are plugged into the plug-in cover 303, and then the liquid collector 3 is sleeved on the support rod 401. During centrifugation, the liquid collector 3 can rotate along the support rod 401 to better adapt to centrifugal force.

[0031] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An AAV purification device, comprising a purification machine (1), a centrifugal assembly (2) and a liquid collector (3), characterized in that: The purifier (1) is internally connected to a centrifugal assembly (2), a plurality of support assemblies (4) are connected to the centrifugal assembly (2), a liquid collector (3) is connected between the plurality of support assemblies (4), a connecting column (5) is connected to the lower end of the centrifugal assembly (2), and a centrifugal motor (6) is connected to the lower end of the connecting column (5); A maintenance plate (101) is connected to one side of the purifier (1), an adjustable foot (102) is connected to the lower end of the purifier (1), a control panel (103) is provided on the front of the purifier (1), a buckle groove (104) is provided on the upper end of the purifier (1) near the front, a pressing groove (109) is provided on the upper end of the purifier (1), and an upturned door (106) is connected to both sides of the pressing groove (109) near the back, an electromagnetic lock (107) is provided on both sides of the lower end of the upturned door (106) near the front, a limit column (105) is provided on both sides of the lower end of the upturned door (106) near the back, a pull plate (108) is provided in the middle of the front of the upturned door (106), and a bearing (110) is provided in the middle of the lower end of the pressing groove (109); The centrifugal assembly (2) is composed of a turntable (201), an upper frame (202), a connecting sleeve (203), a pull groove (204), a pull ring (205), a flap (206) and a limit groove (207), wherein a connecting sleeve (203) is provided in the middle of the upper end of the turntable (201), an upper frame (202) is provided on the upper part of the connecting sleeve (203), a pull ring (205) is provided on the upper end of the connecting sleeve (203), a plurality of groups of limit grooves (207) are provided on the outer side of the upper frame (202), a plurality of groups of flaps (206) are provided on the upper end of the upper frame (202), and a plurality of groups of pull grooves (204) are provided on the upper part of the outer part of the turntable (201); The liquid collector (3) is composed of a liquid collecting box (301), a plug-in slot (302), a plug-in cover (303) and a centrifuge tube (304); the upper end of the liquid collecting box (301) is connected to the plug-in cover (303); the plug-in slot (302) is formed on the outside of the plug-in cover (303); and the upper end of the plug-in cover (303) is connected to a plurality of groups of centrifuge tubes (304); The support assembly (4) is composed of a support rod (401), a telescopic rod (402), a baffle (403), a return spring (404) and a connecting seat (405); one side of the connecting seat (405) is connected to the telescopic rod (402); the return spring (404) is arranged outside the telescopic rod (402); a baffle (403) is arranged on a side of the telescopic rod (402) away from the connecting seat (405); and a support rod (401) is arranged on a side of the baffle (403) away from the telescopic rod (402); An upper bevel gear (501) is provided at the lower end of the connecting column (5); One end of the centrifugal motor (6) is connected to a lower bevel gear (601).

2. An AAV purification device according to claim 1, characterized in that: The purifier (1) and the inspection plate (101) are connected via bolts, and a heat dissipation grid is provided on the inspection plate (101).

3. An AAV purification device according to claim 1, characterized in that: The flip-up door (106) is connected to the purification machine (1) via an electromagnetic lock (107), the limiting column (105) and the purification machine (1) are connected via plugging, and the pull plate (108) and the buckle slot (104) are connected via sleeve connection.

4. An AAV purification device according to claim 1, characterized in that: The connecting column (5) is connected to the purifier (1) via a bearing (110); the upper bevel gear (501) and the lower bevel gear (601) are meshedly connected; the lower bevel gear (601) and the centrifugal motor (6) are connected via a coupling; the lower bevel gear (601) and the purifier (1) are connected via a bearing; and the centrifugal motor (6) and the purifier (1) are connected via a clamp.

5. An AAV purification device according to claim 1, characterized in that: The connecting column (5) and the rotating disk (201) are screwed together, the connecting seat (405) and the purification machine (1) are bolted together, the supporting rod (401) and the purification machine (1) are sleeved together, and the supporting rod (401) and the plug-in slot (302) are plugged together.

6. An AAV purification device according to claim 1, characterized in that: The plug-in cover (303) and the liquid collecting box (301) are screwed together via threads, and the centrifuge tube (304) and the plug-in cover (303) are plugged together.

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