Solid culture medium puncher

Abstract

The utility model discloses a hole puncher for a solid culture medium. The hole puncher comprises a connecting base plate, a push rod is fixedly mounted on the connecting base plate, a punching structure is mounted on the push rod, and an external thread sleeve is mounted on the punching structure; after punching, the movable base plate is pinched and the connecting base plate is pushed, so that the push rod can directly push out residual culture medium blocks in the cavity, the punching head is prevented from being blocked, after the connecting base plate is loosened by a hand, the connecting base plate and the push rod can be pushed to move and reset under the action of the spring, and a space is reserved for the push rod again; during punching, a positioning sleeve rod can be placed at the opening of the culture dish, limitation on a linkage rotating ring is relieved by rotating a screw rod, the linkage rotating ring is rotated to enable multiple pieces of pressing cotton to abut against the opening of the culture dish at the same time, the culture dish and the punching head can be positioned, and the punching head is convenient to use. And deviation of the punching position caused by shaking of an arm or deflection of the culture dish when the punching head is taken is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of microbiological experimental equipment, in particular to a solid culture medium puncher. Background Art

[0002] A solid culture medium puncher is a device used to punch holes in a solid culture medium. Punching holes in the solid culture medium can improve the nutrient intake of microorganisms, accelerate the formation of colonies and increase gas exchange.

[0003] In the prior art, when a hole puncher is used to punch holes in a solid culture medium, remnants of the solid culture medium will remain in the cavity of the punching head, which can easily cause clogging of the punching head when punching multiple times. When punching holes, the puncher needs to be held and inserted into the culture dish. If the hand shakes or the culture dish is tilted by external forces, the punching position will be offset and the punching depth will be affected. Therefore, a solid culture medium puncher is needed to meet people's needs, thereby reducing operating errors, simplifying the operation process, and effectively reducing the probability of contamination during the experiment. Utility Model Content

[0004] The purpose of the utility model is to provide a solid culture medium puncher to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a solid culture medium puncher, comprising a connecting base plate; a push rod is fixedly mounted on the connecting base plate, a punching structure is mounted on the push rod, an external threaded sleeve is mounted on the punching structure, a positioning structure is mounted on the external threaded sleeve, an adjusting ring is mounted on the positioning structure, and a lifting structure is mounted on the adjusting ring;

[0006] The punching structure comprises a movable base plate, which is movably sleeved on the push rod, a punching head is fixedly mounted on one side of the movable base plate, a cavity is formed on the inner wall of the punching head, and the push rod is movably sleeved in the cavity;

[0007] The positioning structure includes a positioning sleeve rod, which is slidably sleeved on an externally threaded sleeve, and the externally threaded sleeve is fixedly sleeved on the punching head. A linkage swivel is rotatably installed on the inner wall of the positioning sleeve rod, and a linkage gear ring is fixedly sleeved on the linkage swivel. A plurality of linkage gear rods are slidably installed on the inner wall of the positioning sleeve rod, and the linkage gear rods are meshed with the linkage gear ring. An arc-shaped abutment plate is fixedly installed on one end of the linkage gear rod, and a pressure cotton is fixedly installed on the arc-shaped abutment plate. An L-shaped connecting frame is fixedly installed on the positioning sleeve rod, a screw rod is threadedly installed on the L-shaped connecting frame, a rubber abutment block is fixedly installed on one end of the screw rod, a rubber ring is fixedly sleeved on the linkage swivel, and the rubber abutment block is in contact with the rubber ring.

[0008] Preferably, the lifting structure includes an annular groove, which is opened on the inner wall of the adjusting ring. A limiting ring is rotatably installed in the annular groove, and the limiting ring is fixedly installed on the positioning sleeve rod. The inner wall of the adjusting ring is opened with an internal adjusting thread, and the external threaded sleeve is threadedly installed in the internal adjusting thread.

[0009] Preferably, the externally threaded sleeve is provided with two sliding grooves, both sliding grooves are provided with scale lines, both sliding grooves are slidably installed with positioning blocks, and both positioning blocks are fixedly installed on the inner wall of the positioning sleeve rod.

[0010] Preferably, a spring is slidably sleeved on the push rod, and both ends of the spring are respectively fixedly mounted on the side where the connecting base plate and the movable base plate are close to each other. A movable hole is opened on the movable base plate, and the push rod is movably mounted in the movable hole.

[0011] Preferably, the inner wall of the positioning sleeve rod is provided with a plurality of guide grooves, and the linkage gear rod is slidably installed in a corresponding guide groove.

[0012] Preferably, both sides of the linkage gear rod are provided with limit sliding grooves, and limit rods are slidably installed in the two limit sliding grooves, and the two limit rods are fixedly installed on the inner walls of both sides of the guide groove respectively.

[0013] Preferably, a threaded adjustment hole is provided on the L-shaped connecting frame, and the screw is threadably installed in the threaded adjustment hole.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] (1) According to the utility model, after punching, by pinching the movable base plate and pushing the connecting base plate, the push rod can directly push out the culture medium residue remaining in the cavity, thereby avoiding blockage of the punching head. When the hand releases the connecting base plate, the connecting base plate and the push rod are pushed to move and reset under the action of the spring, thereby reserving space for the push rod for subsequent punching operations. When punching, the positioning sleeve rod can be placed at the opening of the culture dish, and the restriction on the linkage rotating ring can be released by rotating the screw rod. When the linkage rotating ring is rotated, multiple pressing cottons can be pressed against the opening of the culture dish at the same time, so that the culture dish and the punching head can be positioned, thereby avoiding the position of the punching hole being offset due to arm shaking or the culture dish being tilted when the punching head is taken.

[0016] (2) By turning the adjustment ring, the punching head can be controlled to extend into the culture dish and perform a punching operation on the solid culture medium. The scale line in the sliding groove can be checked to adjust the punching depth. During the process, there is no need to directly take the punching head to operate, so as to avoid drilling too deep due to shaking when manually taking the punching head to punch. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1This is a schematic diagram of the three-dimensional structure of a solid culture medium puncher proposed by the utility model;

[0018] Figure 2 This is a structural schematic diagram of a punching head portion of a solid culture medium puncher proposed by the utility model;

[0019] Figure 3 This is a schematic cross-sectional structural diagram of a punching head portion of a solid culture medium puncher proposed by the utility model;

[0020] Figure 4 This is a schematic diagram of the connection structure of the positioning sleeve rod part of a solid culture medium puncher proposed by the utility model;

[0021] Figure 5 This is a schematic cross-sectional structural diagram of a positioning sleeve rod portion of a solid culture medium puncher proposed by the utility model;

[0022] Figure 6 This is a schematic cross-sectional structure diagram of a linkage rotating ring portion of a solid culture medium puncher proposed by the utility model;

[0023] Figure 7 This is a structural schematic diagram of the linkage gear rod part of a solid culture medium puncher proposed by the utility model.

[0024] In the figure: 1. connecting base plate; 2. push rod; 21. movable base plate; 22. punching head; 23. cavity; 24. spring; 25. movable hole; 3. external threaded sleeve; 31. positioning sleeve rod; 4. linkage swivel; 41. linkage gear ring; 42. linkage gear rod; 43. arc-shaped abutment plate; 44. pressing cotton; 45. L-shaped connecting frame; 46. screw rod; 47. rubber abutment block; 48. rubber ring; 49. guide groove; 410. limiting slide groove; 411. limiting rod; 412. threaded adjustment hole; 5. adjusting ring; 51. annular groove; 52. limiting ring; 53. internal adjustment thread; 54. sliding groove; 55. positioning block. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] Example 1: Please refer to Figure 1-7The utility model provides a technical solution: a solid culture medium puncher, comprising a connecting base plate 1; a push rod 2 is fixedly mounted on the connecting base plate 1, a punching structure is mounted on the push rod 2, an external threaded sleeve 3 is mounted on the punching structure, a positioning structure is mounted on the external threaded sleeve 3, an adjusting ring 5 is mounted on the positioning structure, and a lifting structure is mounted on the adjusting ring 5;

[0027] The punching structure includes a movable base plate 21, which is movably sleeved on the push rod 2. A punching head 22 is fixedly installed on one side of the movable base plate 21. A cavity 23 is formed on the inner wall of the punching head 22. The push rod 2 is movably sleeved in the cavity 23.

[0028] The positioning structure includes a positioning sleeve rod 31, which is slidably sleeved on the external threaded sleeve 3, and the external threaded sleeve 3 is fixedly sleeved on the punching head 22. A linkage swivel 4 is rotatably installed on the inner wall of the positioning sleeve rod 31, and a linkage gear ring 41 is fixedly sleeved on the linkage swivel 4. A plurality of linkage gear rods 42 are slidably installed on the inner wall of the positioning sleeve rod 31, and the linkage gear rods 42 are meshed with the linkage gear ring 41. An arc-shaped abutment plate 43 is fixedly installed on one end of the linkage gear rod 42, and a pressing cotton 44 is fixedly installed on the arc-shaped abutment plate 43. An L-shaped connecting frame 45 is fixedly installed on the positioning sleeve rod 31, and a screw rod 46 is threadedly installed on the L-shaped connecting frame 45. A rubber abutment block 47 is fixedly installed on one end of the screw rod 46. A rubber ring 48 is fixedly sleeved on the linkage swivel 4, and the rubber abutment block 47 contacts with the rubber ring 48. The positioning sleeve rod 31 is placed at the opening of the culture dish, and then the screw 46 is rotated to separate the rubber abutment block 47 from the rubber ring 48. The rubber ring 48 is rotated at this time to make the multiple pressing cottons 44 move outward at the same time, so that the multiple pressing cottons 44 are pressed against the opening of the culture dish at the same time. At the same time, the pressing cottons 44 can adapt to the curvature of the opening of the culture dish, so as to position and connect the punching head 22 and the culture dish to avoid skewed punching due to the shaking of the punching head 22 during punching. After adjustment, the screw rod 46 is rotated in the opposite direction so that the screw rod 46 drives the rubber pressing block 47 to press against the rubber ring 48 again to prevent the rubber ring 48 and the linkage swivel 4 from rotating and loosening. After punching, one hand pinches the movable base plate 21 and presses the connecting base plate 1. The connecting base plate 1 will squeeze the spring 24 and drive the push rod 2 to move in the movable hole 25 on the movable base plate 21. The continuously moving push rod 2 will move in the cavity 23 on the punching head 22. At the same time, the push rod 2 will push out the solid culture medium residue remaining in the cavity 23 to avoid clogging the punching head 22.

[0029] In order to enable the push rod 2 to reset after the remaining debris in the cavity 23 is pushed out and to leave space for the cavity 23 for subsequent punching operations, a spring 24 is slidably sleeved on the push rod 2, and the two ends of the spring 24 are respectively fixedly mounted on the side where the connecting substrate 1 and the movable substrate 21 are close to each other. A movable hole 25 is provided on the movable substrate 21, and the push rod 2 is movably mounted in the movable hole 25. When the connecting substrate 1 is pressed, the spring 24 will be squeezed, and when the connecting substrate 1 is released, the compressed spring 24 will be released and push the connecting substrate 1 to move and reset, so that the connecting substrate 1 drives the push rod 2 to move and reset.

[0030] In order to prevent the linkage gear rod 42 from shifting in position during the sliding process, a plurality of guide grooves 49 are provided on the inner wall of the positioning sleeve rod 31. The linkage gear rod 42 is slidably installed in a corresponding guide groove 49. Limiting grooves 410 are provided on both sides of the linkage gear rod 42. Limiting rods 411 are slidably installed in the two limiting grooves 410. The two limiting rods 411 are respectively fixed on the inner walls of the guide grooves 49 on both sides. The sliding linkage gear rod 42 will slide in the guide grooves 49, thereby limiting the sliding direction of the linkage gear rod 42. The sliding linkage gear rod 42 will slide on the two limiting rods 411 through the two limiting grooves 410, respectively, to prevent the linkage gear rod 42 from shifting during the sliding process.

[0031] In order to facilitate the adjustment of the rubber stopper 47 so that it can move away from or closer to the pressing rubber ring 48 to limit the position of the linkage swivel 4 and prevent the linkage swivel 4 from loosening, a threaded adjustment hole 412 is provided on the L-shaped connecting frame 45, and the screw 46 is threadedly installed in the threaded adjustment hole 412. When the screw 46 rotates, it will rotate and move in the threaded adjustment hole 412, so that the moving screw 46 can drive the rubber stopper 47 to move away from or closer to the pressing rubber ring 48.

[0032] The working principle is as follows: when in use, place the positioning sleeve 31 at the opening of the culture dish, and then rotate the screw 46. When the screw 46 rotates, it will rotate and move in the threaded adjustment hole 412, so that the moving screw 46 drives the rubber block 47 to disengage from the pressure on the rubber ring 48, thereby releasing the limit on the rubber ring 48. At this time, rotate the rubber ring 48, and the rubber ring 48 will drive the linkage swivel 4 to rotate on the inner wall of the positioning sleeve 31. The rotating linkage swivel 4 will drive the linkage gear ring 41 to rotate. When the linkage gear ring 41 rotates, it will drive the linkage gear rod 42 to slide by meshing with the linkage gear rod 42. The sliding linkage gear rod 42 will slide in the guide groove 49, thereby limiting the sliding direction of the linkage gear rod 42. The linkage gear rod 42 will slide on the two limiting rods 411 respectively through the two limiting slide grooves 410 to prevent the linkage gear rod 42 from deviating during the sliding process. The continuously sliding linkage gear rod 42 will drive the arc-shaped abutment plate 43 and the pressing cotton 44 to move. By configuring multiple linkage gear rods 42, arc-shaped abutment plates 43 and pressing cotton 44, multiple pressing cottons 44 can be moved outward at the same time when the linkage swivel 4 is rotated, so that multiple pressing cottons 44 can be pressed against the opening of the culture dish at the same time. At the same time, the pressing cotton 44 can adapt to the curvature of the opening of the culture dish, so as to position and connect the punching head 22 and the culture dish, and avoid the skewed punching caused by the shaking of the punching head 22 during punching. After adjustment, the screw rod 46 is rotated in the opposite direction so that the screw 46 drives the rubber The stop block 47 is pressed against the rubber ring 48 again to prevent the rubber ring 48 and the linkage swivel ring 4 from rotating and loosening, and then the adjusting ring 5 is rotated. The adjusting ring 5 will rotate on the limiting ring 52 through the annular groove 51, so that the adjusting ring 5 can achieve the effect of rotation. The rotating adjusting ring 5 will drive the external threaded sleeve 3 to move vertically downward through the cooperation with the internal adjusting thread 53. The moving external threaded sleeve 3 will slide on the two positioning blocks 55 respectively through the two sliding grooves 54, thereby limiting the moving direction of the external threaded sleeve 3. The vertically descending external threaded sleeve 3 will drive the punching head 22 to descend, so that the punching head 22 extends into the culture dish and punches holes in the solid culture medium. The descent of the punching head 22 is controlled by rotating the adjusting ring 5, and the sliding groove is checked in coordination. The scale lines in 54 are used to adjust the punching depth. After punching, the pressing cotton 44 is loosened from the culture dish and taken out. Then, one hand pinches the movable base plate 21 and presses the connecting base plate 1. The connecting base plate 1 will squeeze the spring 24 and drive the push rod 2 to move in the movable hole 25 on the movable base plate 21. The continuously moving push rod 2 will move in the cavity 23 on the punching head 22. At the same time, the push rod 2 will push out the solid culture medium residues remaining in the cavity 23 to avoid clogging the punching head 22. After pushing out, the connecting base plate 1 is released. At this time, the compressed spring 24 will release and push the connecting base plate 1 to move and reset, so that the connecting base plate 1 drives the push rod 2 to move and reset, and re-reserve space for the cavity 23 for subsequent punching operations.

[0033] Example 2: Figure 1-Figure 6 In order to avoid the difficulty in controlling the drilling depth when manually taking the drilling head 22, and to improve the accuracy of the drilling depth, the lifting structure includes an annular groove 51, which is arranged on the inner wall of the adjusting ring 5, and a limiting ring 52 is rotatably installed in the annular groove 51, and the limiting ring 52 is fixedly installed on the positioning sleeve rod 31. An inner adjusting thread 53 is arranged on the inner wall of the adjusting ring 5, and the external threaded sleeve 3 is threadedly installed in the internal adjusting thread 53. Two sliding grooves 54 are arranged on the external threaded sleeve 3, and scale lines are arranged in the two sliding grooves 54. Positioning blocks 55 are slidably installed in the two sliding grooves 54, and the two positioning blocks 55 are fixedly installed on the inner wall of the positioning sleeve rod 31. The adjusting ring 5 is rotated to adjust The node ring 5 will rotate on the limiting ring 52 through the annular groove 51, so that the adjusting ring 5 can achieve the effect of rotation. The rotating adjusting ring 5 will drive the external threaded sleeve 3 to move vertically downward by cooperating with the internal adjusting thread 53. The moving external threaded sleeve 3 will slide on the two positioning blocks 55 respectively through the two sliding grooves 54, thereby limiting the moving direction of the external threaded sleeve 3. The vertically descending external threaded sleeve 3 will drive the punching head 22 to descend, so that the punching head 22 extends into the culture dish and punches holes in the solid culture medium. The descent of the punching head 22 is controlled by rotating the adjusting ring 5, and the scale line in the sliding groove 54 is checked to adjust the punching depth. The remaining features are the same as those in Example 1.

[0034] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A solid culture medium puncher, comprising a connecting substrate (1); characterized in that: A push rod (2) is fixedly mounted on the connecting base plate (1), a punching structure is mounted on the push rod (2), an external threaded sleeve (3) is mounted on the punching structure, a positioning structure is mounted on the external threaded sleeve (3), an adjusting ring (5) is mounted on the positioning structure, and a lifting structure is mounted on the adjusting ring (5); The punching structure comprises a movable base plate (21), the movable base plate (21) is movably sleeved on the push rod (2), a punching head (22) is fixedly mounted on one side of the movable base plate (21), a cavity (23) is formed on the inner wall of the punching head (22), and the push rod (2) is movably sleeved in the cavity (23); The positioning structure comprises a positioning sleeve rod (31), the positioning sleeve rod (31) is slidably sleeved on the external threaded sleeve (3), the external threaded sleeve (3) is fixedly sleeved on the punching head (22), a linkage rotating ring (4) is rotatably mounted on the inner wall of the positioning sleeve rod (31), a linkage gear ring (41) is fixedly sleeved on the linkage rotating ring (4), a plurality of linkage gear rods (42) are slidably mounted on the inner wall of the positioning sleeve rod (31), and the linkage gear rods (42) are meshed with the linkage gear ring (41). An arc-shaped abutment plate (43) is fixedly mounted on one end of the linkage gear rod (42), a pressing cotton (44) is fixedly mounted on the arc-shaped abutment plate (43), an L-shaped connecting frame (45) is fixedly mounted on the positioning sleeve rod (31), a screw rod (46) is threadedly mounted on the L-shaped connecting frame (45), a rubber abutment block (47) is fixedly mounted on one end of the screw rod (46), a rubber ring (48) is fixedly sleeved on the linkage rotating ring (4), and the rubber abutment block (47) is in contact with the rubber ring (48).

2. A solid culture medium puncher according to claim 1, characterized in that: The lifting structure comprises an annular groove (51) which is provided on the inner wall of the adjusting ring (5); a limiting ring (52) is rotatably mounted in the annular groove (51); the limiting ring (52) is fixedly mounted on the positioning sleeve rod (31); an inner wall of the adjusting ring (5) is provided with an inner adjusting thread (53); and the outer threaded sleeve (3) is threadedly mounted in the inner adjusting thread (53).

3. A solid culture medium puncher according to claim 1, characterized in that: The external threaded sleeve (3) is provided with two sliding grooves (54), each of which is provided with a scale line, and each of which is slidably installed with a positioning block (55), and each of which is fixedly installed on the inner wall of the positioning sleeve rod (31).

4. A solid culture medium puncher according to claim 1, characterized in that: A spring (24) is slidably sleeved on the push rod (2), and two ends of the spring (24) are respectively fixedly mounted on the side where the connecting base plate (1) and the movable base plate (21) are close to each other. A movable hole (25) is opened on the movable base plate (21), and the push rod (2) is movably mounted in the movable hole (25).

5. A solid culture medium puncher according to claim 1, characterized in that: The inner wall of the positioning sleeve rod (31) is provided with a plurality of guide grooves (49), and the linkage gear rod (42) is slidably mounted in a corresponding guide groove (49).

6. A solid culture medium puncher according to claim 1, characterized in that: The linkage gear rod (42) is provided with a limit sliding groove (410) on both sides, and a limit rod (411) is slidably installed in the two limit sliding grooves (410). The two limit rods (411) are respectively fixedly installed on the inner walls on both sides of the guide groove (49).

7. A solid culture medium puncher according to claim 1, characterized in that: The L-shaped connecting frame (45) is provided with a threaded adjustment hole (412), and the screw rod (46) is threadedly installed in the threaded adjustment hole (412).

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