Cell bioreactor

Abstract

The utility model discloses a cell bioreactor, belongs to the technical field of cell reaction, and aims to solve the problems that when a reaction tube is shaken manually, a culture solution and cells cannot be fully mixed and reacted, the density of added cells is low, and when the reaction tube is shaken for a long time, the arm of an operator is prone to ache. Side frames are fixed to the two sides of the top face of the bottom plate respectively, limiting grooves are formed in the tops of the inner side walls of the side frames, a tooth-shaped ring is rotationally connected into the limiting groove in one side, and a limiting ring is rotationally connected into the limiting groove in the other side. A culture solution and cells can be further mixed through the first stirring rod, and the culture solution and the cells entering the two sides of the mixing pipe can be mixed through the second stirring rod, so that the cells and the culture solution are fully contacted, mixed and reacted, and the density of the added cells is improved; the condition that the arm of an operator is prone to ache when the reaction tube is manually shaken for a long time is avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell reaction, and in particular relates to a cell bioreactor. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture technology is an important and commonly used technology in cell biology research methods. Through cell culture, a large number of cells can be obtained, and it can also be used to study cell signal transduction, cell anabolism, cell growth and proliferation, etc.

[0003] At present, the cell culture method of cell reaction tube is mainly used for the culture of suspended cells. During the culture process, the culture medium is placed statically. During the cell growth process, the culture medium is static, and the culture medium is consumed unevenly, thereby limiting the proliferation of cells. Therefore, in order to obtain a higher cell density, the reaction tube needs to be shaken to allow the cells and the culture medium to fully mix and react. However, the current method of shaking the reaction tube is manual shaking. Long-term shaking can easily cause arm pain to the operator, and manual shaking cannot fully mix the culture medium and cells, resulting in a low density of the added cells.

[0004] Therefore, a cell bioreactor is needed to solve the problems in the prior art that when the reaction tube is manually shaken, the culture medium and cells cannot be fully mixed and reacted, resulting in low density of the proliferated cells, and that shaking the reaction tube for a long time easily causes arm pain for the operator. Utility Model Content

[0005] The purpose of the utility model is to provide a cell bioreactor to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell bioreactor, comprising a bottom plate, side frames are respectively fixed on both sides of the top surface of the bottom plate, a limiting groove is opened on the top of the inner wall of the side frame, a toothed ring is rotatably connected in the limiting groove on one side, and a limiting ring is rotatably connected in the limiting groove on the other side, a mixing tube is fixed between the toothed ring and the inner wall of the limiting ring, first fixing rods are respectively arranged on both sides of the middle part of the mixing tube, a plurality of first stirring rods distributed in a circle are fixed on the outer side wall of the first fixing rod, and one end of the first stirring rod is fixed to the inner wall of the mixing tube.

[0007] It should be noted in the scheme that the two side frames are respectively fixed with fixed plates on the top of a side away from each other, a second fixed rod is fixed to the middle of one side of the fixed plate, the second fixed rod is movable through one end of the mixing tube, and a plurality of second stirring rods distributed in a circle are fixed to the outer side wall of the second fixed rod.

[0008] It is further worth mentioning that a discharge pipe is connected through the middle of the bottom surface of the mixing tube, and a second valve is provided on the outer side wall of the discharge pipe.

[0009] It should be further explained that a feed pipe is connected through one side of the top of the outer side wall of the mixing tube, and a first valve is provided on the outer side wall of the feed pipe.

[0010] As a preferred embodiment, a mounting plate is fixed to a side surface of the side frame at one side, a motor is fixed to a top surface of the mounting plate, a gear is fixed to an output end of the motor, and the gear is meshed with the toothed ring.

[0011] Compared with the prior art, the cell bioreactor provided by the utility model has at least the following beneficial effects:

[0012] By setting up the mixing tube, when the bottom of the mixing tube rotates downward from an upward position, the two parts of liquid in the mixing tube can collide with each other and contact with the first stirring rod, so that the cells and the culture fluid are mixed. By setting the first stirring rod, the culture fluid and the cells can be further mixed. By setting the second stirring rod, the culture fluid and cells entering the mixing tube on both sides can be mixed, so that the cells and the culture fluid can fully contact and react, thereby improving the density of the added cells and avoiding the situation where the operator's arm pain is easily caused by manually shaking the reaction tube for a long time. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0014] Figure 2 This is a schematic diagram of the cross-sectional structure of the side frame of the utility model;

[0015] Figure 3 This is a schematic diagram of the cross-sectional structure of the mixing tube of the utility model;

[0016] Figure 4 This is a schematic diagram of the structure of the limit ring of the utility model.

[0017] In the figure:

[0018] 100, bottom plate; 101, side frame; 102, limit groove; 103, toothed ring; 104, limit ring;

[0019] 200, mixing tube; 201, first fixing rod; 202, first stirring rod;

[0020] 300, second fixed rod; 301, second stirring rod;

[0021] 400, feed pipe; 401, first valve;

[0022] 500, discharge pipe; 501, second valve;

[0023] 600, fixed plate;

[0024] 700, mounting plate; 701, motor; 702, gear. DETAILED DESCRIPTION

[0025] See also Figure 1-4 The utility model provides a cell bioreactor, comprising a bottom plate 100, side frames 101 are fixed on both sides of the top surface of the bottom plate 100, a limiting groove 102 is provided on the top of the inner wall of the side frame 101, a toothed ring 103 is rotatably connected in the limiting groove 102 on one side, and a limiting ring 104 is rotatably connected in the limiting groove 102 on the other side, a mixing tube 200 is fixed between the toothed ring 103 and the inner wall of the limiting ring 104, the mixing tube 200 is a V-shaped structure, first fixing rods 201 are respectively arranged on both sides of the middle part of the mixing tube 200, a plurality of first stirring rods 202 distributed in a circumference are fixed to the outer wall of the first fixing rod 201, and one end of the first stirring rod 202 is fixed to the inner wall of the mixing tube 200.

[0026] Further Figure 3 As shown, the two side frames 101 are respectively fixed with a fixing plate 600 at the top of a side away from each other, a second fixing rod 300 is fixed to the middle of a side of the fixing plate 600, the second fixing rod 300 is movable through one end of the mixing tube 200, and a plurality of second stirring rods 301 distributed in a circle are fixed to the outer side wall of the second fixing rod 300.

[0027] By providing the second stirring rod 301, when the mixing tube 200 rotates, the second stirring rod 301 can stir the culture fluid and cells entering the two ends of the mixing tube, so as to further make the cells and the culture fluid fully contact and react.

[0028] Further Figure 3 As shown, a discharge pipe 500 is connected through the middle of the bottom surface of the mixing tube 200 , and a second valve 501 is provided on the outer side wall of the discharge pipe 500 .

[0029] The discharge pipe 500 is provided so that the mixture of the culture solution and cells after the reaction can be taken out from the discharge pipe 500 .

[0030] The present solution has the following working process: when the cells and the culture medium are fully mixed and reacted, firstly, the first valve 401 is opened, and the culture medium is poured into the mixing tube 200, so that the volume of the culture medium reaches the corner positions at both ends of the mixing tube 200. At this time, the culture medium has not filled the mixing tube 200. Then, the cells are poured into the mixing tube 200 from the feeding tube 400, and the first valve 401 is closed. By starting the motor 701, the motor 701 rotates to drive the gear 702 to rotate, the gear 702 rotates to drive the toothed ring 103 to rotate, and the toothed ring 103 rotates to drive the mixing tube 200 to rotate. When the mixing tube 200 rotates, the culture medium and cells move in the mixing tube 200 as the mixing tube 200 rotates. When the bottom of the mixing tube 200 moves upward from the horizontal position, the culture medium and cells are mixed by the mixing tube 200. The V-shaped structure is divided into two parts. The two parts of culture fluid and cells pass through the first fixed rod 201 and the first stirring rod 202 during this process, so that the culture fluid and cells are mixed and stirred. At the same time, the two parts of culture fluid and cells enter the two ends of the mixing tube 200 respectively, and are contacted and stirred with the second stirring rod 301. When the bottom of the mixing tube 200 rotates downward from an upward position, the two parts of liquid in the mixing tube 200 collide with each other and contact with the first stirring rod 202, further mixing the cells and culture fluid. As the mixing tube 200 rotates slowly, the cells can fully contact and react with the culture fluid. When the mixture of the reacted cells and culture fluid is taken out, the mixing tube 200 is in the initial state, the second valve 501 is opened, and the mixture flows from the discharge pipe 500 into the collection device.

[0031] According to the above working process, it can be known that: through the setting of the mixing tube 200, when the bottom of the mixing tube 200 rotates downward from an upward position, the two parts of liquid in the mixing tube 200 can collide with each other and contact with the first stirring rod 202, so that the cells and the culture fluid are mixed, and the setting of the first stirring rod 202 allows the culture fluid and cells to be further mixed, and the setting of the second stirring rod 301 allows the culture fluid and cells entering the two sides of the mixing tube 200 to be mixed, so that the cells and the culture fluid are fully contacted and mixed to react, thereby improving the density of the added cells and avoiding the situation where the operator's arm pain is easily caused by manually shaking the reaction tube for a long time.

[0032] Further as Figure 3 As shown, a feeding pipe 400 is connected through one side of the top of the outer wall of the mixing tube 200 , and a first valve 401 is provided on the outer wall of the feeding pipe 400 .

[0033] The feed tube 400 is provided so that the culture medium and cells can enter the mixing tube 200 .

[0034] Further as Figure 1As shown, a mounting plate 700 is fixed to one side of the side frame 101 , a motor 701 is fixed to the top surface of the mounting plate 700 , a gear 702 is fixed to the output end of the motor 701 , and the gear 702 is meshed with the toothed ring 103 .

[0035] The motor 701 is set to rotate the toothed ring 103, thereby rotating the mixing tube 200. The rotation speed of the motor 701 is set to a rotation speed at which the cells and the culture medium will not rotate at the same speed as the mixing tube 200 due to the centrifugal force when the mixing tube 200 rotates.

[0036] In summary: when the cells and the culture medium are fully mixed for reaction, first open the first valve 401, pour the culture medium into the mixing tube 200, so that the volume of the culture medium reaches the corner positions at both ends of the mixing tube 200. At this time, the culture medium has not filled the mixing tube 200. Then, pour the cells from the feed tube 400 into the mixing tube 200, close the first valve 401, and start the motor 701. The motor 701 rotates to drive the gear 702 to rotate. The gear 702 rotates to drive the toothed ring 103 to rotate. The toothed ring 103 rotates to drive the mixing tube 200 to rotate. When the mixing tube 200 rotates, the culture medium and cells move in the mixing tube 200 as the mixing tube 200 rotates. When the bottom of the mixing tube 200 moves upward from a horizontal position, the culture medium and cells are moved by the V-shaped structure of the mixing tube 200. The culture medium and cells are divided into two parts. During this process, the two parts of culture medium and cells pass through the first fixed rod 201 and the first stirring rod 202, so that the culture medium and cells are mixed and stirred. At the same time, during this process, the two parts of culture medium and cells enter the two ends of the mixing tube 200 respectively, and are contacted and stirred with the second stirring rod 301. When the bottom of the mixing tube 200 rotates downward from an upward position, the two parts of liquid in the mixing tube 200 collide with each other and contact with the first stirring rod 202, further mixing the cells and the culture medium. As the mixing tube 200 rotates slowly, the cells can fully contact and react with the culture medium. When the mixture of the reacted cells and the culture medium is taken out, the mixing tube 200 is in an initial state, the second valve 501 is opened, and the mixture flows from the discharge pipe 500 into the collection device.

[0037] The motor 701 can be purchased from the market, and is a mature technology in this field, which has been fully disclosed, so it will not be repeated in the specification.

[0038] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell bioreactor, comprising a bottom plate (100), characterized in that: Side frames (101) are fixed to both sides of the top surface of the bottom plate (100), and a limiting groove (102) is provided at the top of the inner wall of the side frame (101). A toothed ring (103) is rotatably connected in the limiting groove (102) on one side, and a limiting ring (104) is rotatably connected in the limiting groove (102) on the other side. A mixing tube (200) is fixed between the toothed ring (103) and the inner wall of the limiting ring (104). First fixing rods (201) are provided on both sides of the middle part of the mixing tube (200), and a plurality of first stirring rods (202) distributed in a circumference are fixed to the outer wall of the first fixing rod (201), and one end of the first stirring rod (202) is fixed to the inner wall of the mixing tube (200).

2. A cell bioreactor according to claim 1, characterized in that: A fixing plate (600) is fixed to the top of each of the two side frames (101) which are away from each other. A second fixing rod (300) is fixed to the middle of a side of the fixing plate (600). The second fixing rod (300) movably passes through one end of the mixing tube (200). A plurality of second stirring rods (301) distributed in a circle are fixed to the outer side wall of the second fixing rod (300).

3. A cell bioreactor according to claim 2, characterized in that: A discharge pipe (500) is connected through the middle of the bottom surface of the mixing pipe (200), and a second valve (501) is provided on the outer side wall of the discharge pipe (500).

4. A cell bioreactor according to claim 3, characterized in that: A feed pipe (400) is connected through one side of the top of the outer wall of the mixing pipe (200), and a first valve (401) is provided on the outer wall of the feed pipe (400).

5. A cell bioreactor according to claim 4, characterized in that: A mounting plate (700) is fixed to one side of the side frame (101), a motor (701) is fixed to the top surface of the mounting plate (700), a gear (702) is fixed to the output end of the motor (701), and the gear (702) is meshed with the toothed ring (103).

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