Equipment for concentrating stem cell exosome

Abstract

The equipment for concentrating the stem cell exosomes comprises a storage shell, a rotating mechanism is arranged on the inner wall of the storage shell, a clamping column is fixedly connected to the surface of the rotating mechanism, a clamping groove is formed in one end of the clamping column, a plurality of sets of storage mechanisms for storing the exosomes are arranged above the clamping column, and the clamping groove is formed in the other end of the clamping column. The outer surface of the storage shell is provided with external threads, the upper portion of the storage shell is in threaded connection with a sealing cover, the center of the sealing cover is provided with an opening, and the inner wall of the opening is provided with a refrigeration mechanism. The exosome storage device is reasonable in structure, exosomes are placed in the storage mechanisms correspondingly, then stacked on the clamping columns through the multiple sets of storage mechanisms and clamped through the clamping grooves, then the exosomes are rotated through the rotating mechanism, meanwhile, the temperature of the inner wall of the storage shell is reduced through the refrigeration mechanism, and the exosomes are conveniently stored.

Description

Technical Field

[0001] The utility model relates to the technical field of metal wires, in particular to a device for concentrating stem cell exosomes. Background Art

[0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins. Nowadays, they specifically refer to discoid vesicles with a diameter of 40-100nm. Exosomes were first discovered in sheep reticulocytes. A variety of cells can secrete exosomes under normal and pathological conditions. They are mainly derived from multivesicular bodies formed by the invagination of intracellular lysosomal microparticles. After the multivesicular outer membrane fuses with the cell membrane, it is released into the extracellular matrix.

[0003] All cultured cell types can secrete exosomes, and exosomes are naturally present in body fluids, including blood, saliva, urine, cerebrospinal fluid, and breast milk. The precise molecular mechanisms of their secretion and uptake, as well as their composition, "cargo," and corresponding functions are just beginning to be understood. Exosomes are considered to be specific secreted membrane vesicles that participate in intercellular communication. There is growing interest in the study of exosomes, both to study their functions and to understand how they can be used in the development of minimally invasive diagnostics.

[0004] The exosomes currently produced by the equipment used for mesenchymal stem cell exosomes have low purity and are difficult to preserve for a long time in the hot summer weather. Therefore, a device for concentrating stem cell exosomes is proposed. Utility Model Content

[0005] In order to solve the above problems, the utility model proposes a device for concentrating stem cell exosomes. The technical solution solves the problem that the exosomes produced by the current equipment used for mesenchymal stem cell exosomes mentioned in the above background technology are of low purity and difficult to preserve for a long time in hot summer weather.

[0006] To achieve the above-mentioned object, the utility model provides a device for concentrating stem cell exosomes, comprising a storage shell, the inner wall of which is provided with a rotating mechanism, a surface of which is fixedly connected with a clamping column, and one end of which is provided with a clamping groove;

[0007] A plurality of storage mechanisms for storing exosomes are provided above the clamping column, an external thread is provided on the outer surface of the storage shell, a sealing cover is threadedly connected to the upper part of the storage shell, an opening is provided at the center of the sealing cover, and a refrigeration mechanism is provided on the inner wall of the opening.

[0008] Optionally, the rotating mechanism includes a motor fixedly connected to the center of the inner wall of the storage shell, a slide rail fixedly connected to the inner wall of the storage shell, and a connecting frame fixedly connected to one side of the slide rail;

[0009] The output end of the motor is fixedly connected to the connecting frame.

[0010] Optionally, the storage mechanism includes a culture dish, a plurality of holding posts fixedly connected to the inner wall of the culture dish, and a plurality of hexagonal card blocks fixedly connected to the bottom of the culture dish;

[0011] A clamping groove is provided at one end of the clamping column for matching with the hexagonal clamping block.

[0012] Optionally, the refrigeration mechanism includes a connecting frame fixedly connected to the cooling shell, a connecting frame fixedly connected to the inner wall of the cooling shell, a semiconductor refrigerator fixedly connected to the inner wall of the connecting frame, and heat dissipation fins fixedly connected to the heating surface of the semiconductor refrigerator.

[0013] Optionally, a plurality of air inlets are provided on the surface of the cooling shell.

[0014] Optionally, a mounting opening is provided on the surface of the cooling shell, and a heat dissipation fan is fixedly connected to the inner wall of the mounting opening.

[0015] Optionally, the cross-sectional shape of the snap-fit ​​groove is hexagonal.

[0016] Optionally, a gasket is fixedly connected to the bottom of the storage shell.

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] The present application has a reasonable structure. The exosomes are placed inside the storage mechanism respectively, and then stacked on the snap-in columns through multiple groups of storage mechanisms and snap-in through the snap-in grooves. The rotating mechanism is then used to rotate the exosomes, and the temperature of the inner wall of the storage shell is lowered by the refrigeration mechanism, so as to facilitate the storage of the exosomes.

[0019] In order to better understand and implement the present invention, the present invention is described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The three-dimensional structure of the utility model is shown in FIG. Figure 1 ;

[0021] Figure 2 The three-dimensional structure of the utility model is shown in FIG. Figure 2 ;

[0022] Figure 3 It is a schematic diagram of the cross-sectional structure of the storage shell in the utility model;

[0023] Figure 4 It is a schematic diagram of the cross-sectional structure of the cooling shell in the utility model.

[0024] In the figure: 1. storage shell; 2. sealing cover; 3. cooling shell; 4. heat dissipation fan; 5. gasket; 6. slide rail; 7. connecting frame; 8. motor; 9. clamping column; 10. culture dish; 11. clamping column; 12. connecting frame; 13. semiconductor refrigerator; 14. heat dissipation fins; 15. installation port; 16. air inlet. DETAILED DESCRIPTION

[0025] In order to have a clearer understanding of the technical features, purposes and effects of the utility model, the specific implementation methods of the utility model are now described with reference to the accompanying drawings, but the protection scope of the utility model is not limited to the following.

[0026] Reference Figure 1-4 As shown, a device for concentrating stem cell exosomes comprises a storage shell 1, the inner wall of the storage shell 1 is provided with a rotating mechanism, a clamping column 9 is fixedly connected to the surface of the rotating mechanism, and a clamping groove is provided at one end of the clamping column 9;

[0027] A plurality of storage mechanisms for storing exosomes are provided above the clamping column 9. The outer surface of the storage shell 1 is provided with an external thread. The upper part of the storage shell 1 is threadedly connected with a sealing cover 2. The center of the sealing cover 2 is provided with an opening, and the inner wall of the opening is provided with a refrigeration mechanism.

[0028] When in use, the exosomes are placed inside the storage mechanism respectively, and then stacked on the snap-in column 9 through multiple sets of storage mechanisms and snapped through the snap-in grooves. The rotating mechanism is then used to rotate it, and at the same time, the temperature of the inner wall of the storage shell 1 is lowered through the refrigeration mechanism to facilitate the storage of the exosomes.

[0029] Specifically, the rotating mechanism includes a motor 8 fixedly connected to the center of the inner wall of the storage shell 1, a slide rail 6 fixedly connected to the inner wall of the storage shell 1, and a connecting frame 7 fixedly connected to one side of the slide rail 6;

[0030] The output end of the motor 8 is fixedly connected to the connecting frame 7;

[0031] The motor 8 drives the connecting frame 7 to rotate on the surface of the slide rail 6, thereby driving the storage mechanism to rotate synchronously through the clamping column 9.

[0032] Specifically, the storage mechanism includes a culture dish 10, a plurality of holding columns 11 fixedly connected to the inner wall of the culture dish 10, and a plurality of hexagonal card blocks fixedly connected to the bottom of the culture dish 10;

[0033] A clamping groove is provided at one end of the clamping column 11 for matching with the hexagonal clamping block;

[0034] The hexagonal clamping blocks facilitate the clamping and stacking of the culture dishes 10 , and at the same time, the bottom hexagonal clamping block is clamped with the clamping groove at one end of the clamping column 9 to achieve a fixing effect.

[0035] Specifically, the refrigeration mechanism includes a connection frame 12 fixedly connected to the cooling shell 3, fixedly connected to the inner wall of the cooling shell 3, a semiconductor refrigerator 13 fixedly connected to the inner wall of the connection frame 12, and a heat dissipation fin 14 fixedly connected to the heating surface of the semiconductor refrigerator 13;

[0036] A plurality of air inlets 16 are provided on the surface of the cooling shell 3, a mounting opening 15 is provided on the surface of the cooling shell 3, and a heat dissipation fan 4 is fixedly connected to the inner wall of the mounting opening 15;

[0037] The temperature inside the storage shell 1 is lowered by the semiconductor refrigerator 13 , and the heat dissipation fan 4 is combined with the heat dissipation fins 14 to dissipate the heat.

[0038] Specifically, the cross-sectional shape of the clamping groove is hexagonal, and the hexagonal shape facilitates the clamping of the hexagonal clamping block.

[0039] Specifically, a gasket 5 is fixedly connected to the bottom of the storage shell 1 , and the gasket 5 is in contact with the ground to protect the storage shell 1 .

[0040] The above disclosure is only a preferred embodiment of the present invention, which certainly cannot be used to limit the scope of rights of the present invention. Therefore, equivalent changes made according to the claims of the present invention still fall within the scope covered by the present invention.

Claims

1. A device for concentrating stem cell exosomes, characterized in that: It comprises a storage shell (1), the inner wall of the storage shell (1) is provided with a rotating mechanism, a clamping column (9) is fixedly connected to the surface of the rotating mechanism, and a clamping groove is provided at one end of the clamping column (9); A plurality of storage mechanisms for storing exosomes are arranged above the clamping column (9); an external thread is provided on the outer surface of the storage shell (1); a sealing cover (2) is threadedly connected to the upper portion of the storage shell (1); an opening is provided at the center of the sealing cover (2); and a refrigeration mechanism is provided on the inner wall of the opening.

2. The device for concentrating stem cell exosomes according to claim 1, characterized in that: The rotating mechanism comprises a motor (8) fixedly connected to the center of the inner wall of the storage shell (1), a slide rail (6) fixedly connected to the inner wall of the storage shell (1), and a connecting frame (7) fixedly connected to one side of the slide rail (6); The output end of the motor (8) is fixedly connected to the connecting frame (7).

3. The device for concentrating stem cell exosomes according to claim 1, characterized in that: The storage mechanism comprises a culture dish (10), a plurality of holding columns (11) fixedly connected to the inner wall of the culture dish (10), and a plurality of hexagonal holding blocks fixedly connected to the bottom of the culture dish (10); One end of the clamping column (11) is provided with a clamping groove for matching with a hexagonal clamping block.

4. The device for concentrating stem cell exosomes according to claim 1, characterized in that: The refrigeration mechanism comprises a connection frame (12) fixedly connected to a cooling shell (3), fixedly connected to the inner wall of the cooling shell (3), a semiconductor refrigerator (13) fixedly connected to the inner wall of the connection frame (12), and heat dissipation fins (14) fixedly connected to the heating surface of the semiconductor refrigerator (13).

5. The device for concentrating stem cell exosomes according to claim 4, characterized in that: A plurality of air inlets (16) are provided on the surface of the cooling shell (3).

6. The device for concentrating stem cell exosomes according to claim 4, characterized in that: The surface of the cooling shell (3) is provided with a mounting opening (15), and the inner wall of the mounting opening (15) is fixedly connected with a heat dissipation fan (4).

7. The device for concentrating stem cell exosomes according to claim 1, characterized in that: The cross-section of the clamping groove is hexagonal.

8. The device for concentrating stem cell exosomes according to claim 1, characterized in that: A gasket (5) is fixedly connected to the bottom of the storage shell (1).

Images