Stem cell exosome filtering device
By designing a stem cell exosome filtration device, the regulation structure prevents air pollutants from entering and limiting structure improves pipeline stability, solves the pollution problem in stem cell culture and improves the quality of cell filtration.
Patent Information
- Application Number
- CN202421092635.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-20
- Publication Date
- 2025-01-14
- Estimated Expiration
- 2034-05-20
AI Technical Summary
During stem cell culture, bacteria or other contaminants in the air may enter the culture flask, causing the cell culture liquid to be contaminated and affect the cell quality and results.
A stem cell exosome filtration device is designed, including a flat filter and a regulatory structure. By setting up a regulatory structure, the bottle mouth of the culture bottle is fitted to the surface of the flat filter to avoid bacteria in the air and improve the pipeline through the limit structure. The stability ensures the quality of stem cell filtration.
Effectively prevent bacteria and pollutants from entering the culture bottle, improve the quality and effectiveness of stem cell filtration, and ensure the purity and stability of cell culture.
Smart Images
Figure CN222342082U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of stem cell exosomes, in particular to a stem cell exosome filtering device. Background Art
[0002] Stem cells are cells with the potential for self-renewal and differentiation. Stem cell exosomes are extracellular vesicles released by stem cells that contain a variety of biologically active substances. They are mainly wrapped by a lipid bilayer and contain a variety of biologically active substances, such as proteins, nucleic acids, cytokines, etc. Exosomes have multiple functions, including promoting cell proliferation, apoptosis, repair and regeneration, regulating immune responses, cell signal transduction, etc.
[0003] Existing related technologies often have the following defects: filtration is required during the process of stem cell culture. The stem cells are filtered by using a constant flow pump with a flat filter. During the process of stem cell filtration, the culture bottle for collecting the filtered cells is usually placed directly below the drain port. Bacteria or other contaminants in the air may cause the collected stem cell culture fluid to be contaminated, thereby affecting the quality and results of cell culture.
[0004] Therefore, the utility model provides a stem cell exosome filtering device. Utility Model Content
[0005] The utility model aims to solve the shortcoming in the prior art that bacteria or other pollutants in the air may enter the culture bottle, and proposes a stem cell exosome filtering device.
[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a stem cell exosome filtration device, comprising a flat filter, a plurality of legs are fixedly connected to the lower surface of the flat filter, a liquid inlet pipe is connected to the upper surface of the flat filter, a liquid discharge pipe is connected to the lower surface of the flat filter, an adjustment structure is provided on the surface of the flat filter, the adjustment structure comprises a frame, the frame is fixedly connected to the surface of the flat filter, a driving arm is slidably connected to the inner wall of the frame, a bottom plate is fixedly connected to the surface of the driving arm, a latch is slidably inserted into the surface of the frame, a circular groove is provided on the surface of the driving arm, and the size of the latch matches the size of the circular groove.
[0007] The effect achieved by the above components is: by setting the adjustment structure, it is easy to fit the bottle mouth of the culture bottle on the surface of the flat filter, so that the culture bottle is placed on the surface of the drainage tube, thereby preventing bacteria and pollutants in the air from entering the culture bottle when collecting filtered stem cells, thereby improving the quality of filtered cells.
[0008] Preferably, a rubber ring is fixedly connected to the surface of the flat plate filter, and an arc groove is formed on the surface of the rubber ring.
[0009] The effect achieved by the above components is: after the culture bottle is moved to a suitable position, it will fit on the surface of the rubber ring, and the mouth of the culture bottle will be stuck in the arc groove. The rubber ring can improve the airtightness of the fit between the culture bottle and the flat filter, thereby further protecting the filtered stem cells.
[0010] Preferably, the arc surface of the latch is sleeved with a spring, and two ends of the spring are fixedly connected to the latch and the frame respectively.
[0011] The effect achieved by the above components is that the bolt is inserted into the circular groove with the help of the contraction force of the spring, and the spring achieves the effect of improving the stability of the bolt inserted into the circular groove and preventing the bolt from falling out of the circular groove.
[0012] Preferably, a plurality of limit blocks are fixedly connected to the surface of the bottom plate, and the cross-section of the limit blocks is trapezoidal.
[0013] The effect achieved by the above components is that the culture bottle will fit on the surface of the limiting block, and the limiting block reaches the position to limit the culture bottle, so that the culture bottle is placed in the center position of the bottom plate.
[0014] Preferably, a limiting structure is provided on the surface of the flat filter, the limiting structure comprises a bracket, the surface of the bracket is rotatably connected to a bidirectional screw, the surface of the bidirectional screw is threadedly connected to two limiting arms, and the two limiting arms are fixedly connected to an arc plate on one side close to each other.
[0015] The effect achieved by the above components is: by setting a limiting structure, the stability of the pipeline sleeved on the surface of the liquid inlet pipe is improved, thereby avoiding excessive pressure in the pipeline to transport stem cells, causing the pipeline to detach from the surface of the liquid inlet pipe, thereby improving the effect of stem cell filtration.
[0016] Preferably, a round rod is slidably penetrated through the surface of the limiting arm, and one end of the round rod is fixedly connected to the surface of the bracket.
[0017] The effect achieved by the above components is that the two driving arms will slide along the surface of the round rod when they move, and the round rod can limit the moving path of the driving arms, thereby preventing the driving arms from rotating when moving.
[0018] Preferably, a turntable is fixedly connected to the surface of the bidirectional screw, and a plurality of anti-slip strips are fixedly connected to the sides of the two arc-shaped plates close to each other.
[0019] The effects achieved by the above components are: the rotation of the turntable will drive the bidirectional screw to rotate, the turntable facilitates the rotation of the bidirectional screw, the arc plate will drive the anti-slip strip to fit the surface of the pipe, and the anti-slip strip can enhance the effect of the limit plate restricting the pipe.
[0020] In summary:
[0021] 1. In the utility model, by setting an adjustment structure, the culture bottle is placed on the surface of the bottom plate, and the driving arm is pulled. The movement of the driving arm will drive the bottom plate to move, and the movement of the bottom plate will drive the culture bottle to move in the direction close to the drainage pipe. After the culture bottle moves to a suitable position, the bottle mouth will fit on the surface of the flat filter, thereby preventing bacteria and pollutants in the air from entering the culture bottle when collecting filtered stem cells.
[0022] 2. In the utility model, a limiting structure is provided to achieve the goal of sleeveing the pipe on the surface of the liquid inlet pipe, and rotating the bidirectional screw. The rotation of the bidirectional screw will drive the two driving arms toward each other with the help of the thread. When the two driving arms move, they will drive the two arc plates toward each other. After the arc plates move to a suitable position, they will squeeze the pipe, thereby limiting the pipe on the surface of the liquid inlet pipe, thereby improving the stability of the pipe sleeved on the surface of the liquid inlet pipe. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;
[0024] Figure 2 It is a structural schematic diagram of the utility model from another angle;
[0025] Figure 3 It is a structural schematic diagram of the regulating structure of the utility model;
[0026] Figure 4 It is a structural schematic diagram of the limiting structure of the utility model.
[0027] Legend: 1. Flat filter; 2. Support legs; 3. Liquid inlet; 4. Liquid outlet; 5. Adjustment structure; 51. Frame; 52. Drive arm; 53. Bottom plate; 54. Latch; 55. Round groove; 56. Rubber ring; 57. Arc groove; 58. Spring; 59. Limit block; 6. Limit structure; 61. Bracket; 62. Bidirectional screw; 63. Limit arm; 64. Arc plate; 65. Round rod; 66. Turntable; 67. Anti-slip strip. DETAILED DESCRIPTION
[0028] Reference Figure 1As shown, the utility model provides a technical solution: a stem cell exosome filtration device, comprising a flat filter 1, a plurality of legs 2 are fixedly connected to the lower surface of the flat filter 1, a liquid inlet pipe 3 is connected to the upper surface of the flat filter 1, a liquid discharge pipe 4 is connected to the lower surface of the flat filter 1, and an adjustment structure 5 is provided on the surface of the flat filter 1. By providing the adjustment structure 5, it is convenient to fit the bottle mouth of the culture bottle to the surface of the flat filter 1, so that the culture bottle is sleeved on the surface of the liquid discharge pipe 4, thereby avoiding the bacteria and pollutants in the air from entering the culture bottle when collecting the filtered stem cells, thereby improving the quality of the filtered cells. A limiting structure 6 is provided on the surface of the flat filter 1, and by providing the limiting structure 6, the stability of the pipeline sleeved on the surface of the liquid inlet pipe 3 is improved, thereby avoiding the situation that the pipeline is detached from the surface of the liquid inlet pipe 3 due to excessive pressure in the pipeline transporting stem cells, thereby improving the effect of stem cell filtration.
[0029] The specific settings and functions of the adjustment structure 5 and the limiting structure 6 are described in detail below.
[0030] Reference Figure 2 and Figure 3 As shown, in this embodiment: the adjustment structure 5 includes a frame 51, the frame 51 is fixedly connected to the surface of the flat filter 1, the inner wall of the frame 51 is slidably connected to a driving arm 52, the surface of the driving arm 52 is fixedly connected to a bottom plate 53, the surface of the frame 51 is slidably inserted with a latch 54, the surface of the driving arm 52 is provided with a circular groove 55, and the size of the latch 54 matches the size of the circular groove 55. A rubber ring 56 is fixedly connected to the surface of the flat filter 1, and the surface of the rubber ring 56 is provided with an arc groove 57. After the culture bottle is moved to a suitable position, it will fit on the surface of the rubber ring 56, and the bottle mouth of the culture bottle will be stuck in the arc groove 57. The rubber ring 56 can improve the airtightness of the fit between the culture bottle and the flat filter 1, thereby further protecting the filtered stem cells. The arc surface of the latch 54 is covered with a spring 58, and the two ends of the spring 58 are fixedly connected to the latch 54 and the frame 51 respectively. The latch 54 will be inserted into the circular groove 55 with the help of the contraction force of the spring 58. The spring 58 can improve the stability of the latch 54 inserted into the circular groove 55 and prevent the latch 54 from falling out of the circular groove 55. The surface of the bottom plate 53 is fixedly connected with a plurality of limit blocks 59, and the cross section of the limit blocks 59 is trapezoidal. The culture bottle will fit on the surface of the limit blocks 59, and the limit blocks 59 can limit the position of the culture bottle, so that the culture bottle is placed in the center of the bottom plate 53.
[0031] Reference Figure 4As shown, specifically, the limiting structure 6 includes a bracket 61, the surface of the bracket 61 is rotatably connected with a bidirectional screw 62, the surface of the bidirectional screw 62 is threadedly connected with two limiting arms 63, and the two limiting arms 63 are fixedly connected with an arc plate 64 on the side close to each other. A round rod 65 is slidably penetrated through the surface of the limiting arm 63, one end of the round rod 65 is fixedly connected to the surface of the bracket 61, and the two driving arms 52 will slide along the surface of the round rod 65 when moving, and the round rod 65 can limit the moving path of the driving arm 52, thereby preventing the driving arm 52 from rotating when moving. A rotating disk 66 is fixedly connected to the surface of the bidirectional screw 62, and the two arc plates 64 are fixedly connected with a plurality of anti-slip strips 67 on the side close to each other, and the rotation of the rotating disk 66 will drive the bidirectional screw 62 to rotate, and the rotating disk 66 can facilitate the rotation of the bidirectional screw 62, and the arc plate 64 will drive the anti-slip strip 67 to fit on the surface of the pipeline, and the anti-slip strip 67 can improve the effect of the limiting plate limiting the pipeline.
[0032] Working principle: Exosomes can be secreted by a variety of cell types, including stem cells, tumor cells, immune cells, etc. When the filtered stem cell exosomes need to be collected, the culture bottle is first placed on the surface of the bottom plate 53. The culture bottle will fit on the surface of the limit block 59. The limit block 59 reaches the position to limit the culture bottle, so that the culture bottle is placed in the center of the bottom plate 53. Then, the latch 54 is pulled. The latch 54 moves in the frame 51 and gradually comes out of the circular groove 55. The movement of the latch 54 will stretch the spring 58. At this time, the spring 58 is in a stretched state. When the latch 54 comes out of the circular groove 55, the spring 58 is stretched. 5, the driving arm 52 is pulled, and the movement of the driving arm 52 drives the bottom plate 53 to move, and the movement of the bottom plate 53 drives the culture bottle to move toward the direction close to the drain pipe 4. After the culture bottle moves to a suitable position, it fits on the surface of the rubber ring 56, and the bottle mouth of the culture bottle is stuck in the arc groove 57. The rubber ring 56 improves the airtightness of the fit between the culture bottle and the flat filter 1, thereby further protecting the filtered stem cells, and then the latch 54 is released. The latch 54 is inserted into the circular groove 55 with the help of the contraction force of the spring 58, thereby limiting the position of the driving arm 52, and further limiting the position of the culture bottle.
[0033] When it is necessary to limit the position of the pipe connected to the liquid inlet pipe 3, first, the pipe is put on the surface of the liquid inlet pipe 3, and then the turntable 66 is rotated. The rotation of the turntable 66 will drive the bidirectional screw 62 to rotate, and the rotation of the bidirectional screw 62 will rotate in the bracket 61. The rotation of the bidirectional screw 62 will drive the two driving arms 52 to approach each other with the help of the thread. The movement of the two driving arms 52 will slide along the surface of the round rod 65, and the round rod 65 will limit the movement path of the driving arms 52, thereby preventing the driving arms 52 from rotating when moving. When the two driving arms 52 move, they will drive the two arc plates 64 to move in a direction close to each other, and the arc plates 64 will drive the anti-slip strips 67 to fit the surface of the pipe. The arc plates 64 will squeeze the pipe, thereby limiting the pipe on the surface of the liquid inlet pipe 3.
[0034] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.
Claims
1. A stem cell exosome filtration device, comprising a flat plate filter (1), wherein a plurality of legs (2) are fixedly connected to the lower surface of the flat plate filter (1), an upper surface of the flat plate filter (1) is connected to a liquid inlet pipe (3), and a lower surface of the flat plate filter (1) is connected to a liquid discharge pipe (4), and an adjustment structure (5) is provided on the surface of the flat plate filter (1), characterized in that: The regulating structure (5) comprises a frame (51), the frame (51) is fixedly connected to the surface of the flat plate filter (1), a driving arm (52) is slidably connected to the inner wall of the frame (51), a bottom plate (53) is fixedly connected to the surface of the driving arm (52), a latch (54) is slidably inserted into the surface of the frame (51), a circular groove (55) is formed on the surface of the driving arm (52), and the size of the latch (54) is matched with the size of the circular groove (55).
2. A stem cell exosome filtration device according to claim 1, characterized in that: A rubber ring (56) is fixedly connected to the surface of the flat plate filter (1), and an arc-shaped groove (57) is formed on the surface of the rubber ring (56).
3. A stem cell exosome filtration device according to claim 1, characterized in that: The arc surface of the latch (54) is sleeved with a spring (58), and the two ends of the spring (58) are respectively fixedly connected to the latch (54) and the frame (51).
4. The stem cell exosome filtration device according to claim 1, characterized in that: A plurality of limit blocks (59) are fixedly connected to the surface of the bottom plate (53), and the cross section of the limit blocks (59) is trapezoidal.
5. The stem cell exosome filtration device according to claim 1, characterized in that: The surface of the flat plate filter (1) is provided with a limiting structure (6), the limiting structure (6) comprising a bracket (61), the surface of the bracket (61) being rotatably connected to a bidirectional screw (62), the surface of the bidirectional screw (62) being threadedly connected to two limiting arms (63), and the two limiting arms (63) are fixedly connected to an arc plate (64) on one side close to each other.
6. A stem cell exosome filtration device according to claim 5, characterized in that: A round rod (65) is slidably penetrated through the surface of the limiting arm (63), and one end of the round rod (65) is fixedly connected to the surface of the bracket (61).
7. The stem cell exosome filtration device according to claim 5, characterized in that: A rotating disk (66) is fixedly connected to the surface of the bidirectional screw rod (62), and a plurality of anti-slip strips (67) are fixedly connected to the sides of the two arc-shaped plates (64) close to each other.