Tip head movement assembly of nucleic acid extraction and detection all-in-one machine
By designing the tip head movement component on the nucleic acid extraction and detection machine, the automated tip head and gun head are realized, solving the problem of low automation level in the prior art and improving the efficiency and accuracy of the experiment.
Patent Information
- Application Number
- CN202420737301.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-10
- Publication Date
- 2025-01-14
- Estimated Expiration
- 2034-04-10
AI Technical Summary
The existing nucleic acid extraction and detection all-in-one machines have low automation level in the automatic binding and separation of tip heads, which is prone to failure in binding, affecting the detection process.
A tip head movement assembly is designed, including combining the movement assembly and the separation of the movement assembly, which can achieve automated docking and separation through the gun tip and the tip head sensing assembly, and combine the movement assembly to combine the tip head with the gun tip, and separate the movement assembly by ejecting the fork. The rod prevents the tip head from moving, and the sensing assembly senses the combined state of the tip head with the gun head through the sensor and the optocoupler.
The automation level of the nucleic acid extraction and detection machine is improved, the correct combination and separation of tip heads is ensured, and the efficiency and accuracy of the experiment are improved.
Smart Images
Figure CN222348978U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of biotechnology, in particular to a component of a nucleic acid extraction and detection device, and in particular to a tip head movement component of an integrated nucleic acid extraction and detection machine. Background Art
[0002] Nucleic acid extraction and amplification detection are important experimental methods in molecular biology research. Early experiments were all done manually, which took a long time and the experimental results were easily affected by individual differences in experimental personnel. With the development of industrial automation, in the field of experimental science, traditional manual experiments are increasingly replaced by automated equipment. The repeatability and consistency of automated equipment reduce the errors of traditional manual experiments and improve the reliability and efficiency of experiments. The nucleic acid extraction and detection all-in-one machine is a highly integrated compact instrument that performs fully automatic, fully closed, and integrated operations for nucleic acid extraction and amplification. It can highly automate the automation of nucleic acid extraction and amplification detection, shorten the nucleic acid extraction and amplification process, reduce the impact of human factors, enhance the safety and effectiveness of nucleic acid sample preparation, and realize the miniaturization and portability of the device to meet the needs of grassroots or on-site rapid detection. It is the main research direction for the development of new nucleic acid extraction and detection all-in-one machines.
[0003] The nucleic acid extraction and detection integrated machine needs to take out the pipette tip to realize the pipetting and reaction of the sample to be tested. The pipette tip is a disposable pipette tip (tip head) and is a disposable consumable. Therefore, the nucleic acid extraction and detection integrated machine needs to be provided with a tip head to take out and remove the tip head, as well as related components that can drive the tip head to realize pipetting, so as to achieve smooth docking with the tip head. Among the existing nucleic acid extraction instruments, some are directly combined with the tip head by manual operation, and the separation after use is completed. The manual operation affects the efficiency of nucleic acid extraction and reduces the automation level; some nucleic acid extraction instruments are only provided with related equipment for taking the tip head, but there is no sensing device for whether the extraction is successful, or there is no device for removing the tip head. The automation level is low, and the tip head is prone to failure in combination, thereby affecting the detection process. Therefore, there is an urgent need for a device that can smoothly realize the automatic combination and separation of the nucleic acid extraction and detection integrated machine and the tip head, and can also sense whether the combination is successful, so as to improve the working efficiency of the nucleic acid extraction and detection integrated machine. Utility Model Content
[0004] In view of the problems existing in the prior art, the utility model provides a tip head motion component of a nucleic acid extraction and detection integrated machine, including a tip head combined motion component and a separation motion component assembled into one body, the combined motion component can make the tip head combined with the gun head, so that the gun head drives the tip head to move, the separation motion component stops the tip head from moving by popping out the fork rod, so that the tip head is separated from the gun head that continues to move, and the gun head can be replaced and combined with a new tip head, and a tip head sensing component is also provided to sense whether the tip head is successfully combined with the gun head. This component improves the automation level of the nucleic acid extraction and detection integrated machine, prevents errors in the process of taking off the tip head, and ensures the sensitivity and accuracy of nucleic acid detection.
[0005] The tip head described in the utility model is a disposable liquid transfer gun head, which is made of plastic.
[0006] The nucleic acid extraction and detection integrated machine needs to use the tip head to absorb, pipette, mix and react multiple reagents to achieve nucleic acid extraction, purification and amplification. In order to realize the automatic docking between the nucleic acid extraction and detection integrated machine and the tip head, the utility model provides a tip head motion component of the nucleic acid extraction and detection integrated machine, the tip head motion component includes a combination motion component and a separation motion component; the combination motion component can combine the tip head with the gun head, so that the gun head drives the tip head to move; the separation motion component prevents the tip head from moving by popping out the fork rod, so that the tip head is separated from the gun head that continues to move.
[0007] Furthermore, the combined motion component includes a gun head and a tip head sensing component; the tip head sensing component includes a gun head sliding sleeve and a sensor for sensing whether the tip head is successfully combined with the gun head.
[0008] Furthermore, the gun head slide can be movably mounted outside the gun head. When the gun head takes the tip head, the tip head pushes the gun head slide to move upward, and the upper end of the gun head slide contacts the sensor, triggering the sensor signal, indicating that the tip head and the gun head are successfully combined.
[0009] Furthermore, a trigger optical coupler is provided at the upper end of the gun head slide. When the gun head slide moves upward, the optical coupler contacts the sensor, and the sensor sends a signal indicating that the tip head is combined with the gun head.
[0010] Furthermore, the separation motion assembly pops out the shift fork rod, and under the restriction of the shift fork rod, the tip head stops moving upward, thereby separating the tip head from the gun head that continues to move upward.
[0011] Furthermore, the shift fork rod is controlled by an electromagnet to pop out or retract. When the electromagnet is energized in one direction, the shift fork rod pops out, and when the electromagnet is energized in the opposite direction, the shift fork rod is retracted.
[0012] In some embodiments, when the electromagnet is energized from one direction, magnetism is generated, causing the fork rod to be pushed out due to the repulsion of like poles of magnetism; when the electromagnet is energized from another direction, opposite magnetism is generated, causing the fork rod to be retracted due to the attraction of opposite poles of magnetism.
[0013] Furthermore, two notches are provided at the lower end of the gun head slide. When the fork rod is extended, it enters the two notches at the lower end of the gun head slide, thereby stopping the tip head from moving upward and detaching from the gun head.
[0014] In some embodiments, the fork rod is in a Y shape. When the fork rod is extended, the upper ends of the Y shape can be inserted into the two notches at the lower end of the gun head slide, so that the fork rod can directly contact the upper end of the tip head, causing the tip head to detach from the gun head and fall off.
[0015] Furthermore, the gun head includes a long rod and a ball head, the ball head is fixed to the lower end of the long rod, and when the ball head is embedded in the upper end of the tip head, the tip head and the gun head are combined.
[0016] Furthermore, a first annular ridge is provided on the upper end of the ball head, and a second annular ridge is also provided on the long rod above the ball head. The first annular ridge and the second annular ridge are used to help fix the tip head so that the tip head and the gun head are more firmly combined.
[0017] The two annular ridges can be designed to match the upper end of the tip head, so that the tip head will not fall off after being combined with the gun head.
[0018] Furthermore, a limiting device for a gun tip slide is provided at the upper end of the long rod, and the limiting device includes a spring and a baffle; the upper end of the spring is fixed to the outside of the long rod to prevent the gun tip slide from moving upward, and the baffle can resist the edge of the upper end of the gun tip slide to prevent the gun tip slide from moving downward and separating from the gun tip.
[0019] The limit device can limit the highest and lowest positions of the gun head slide, so that the gun head slide is always outside the long rod, wrapping the long rod and will not fall off. At the same time, the design of the spring can give the gun head slide a certain range of upward movement space. When the tip head is combined with the gun head, it can push the gun head slide upward and the spring is compressed; when the tip head is separated from the gun head, the gun head slide moves down due to gravity, is held and fixed by the baffle and is in the lowest position, and the spring recovers.
[0020] In summary, the beneficial effects of the utility model are as follows: by arranging the combining motion component and the separating motion component of the tip head on the nucleic acid extraction and detection integrated machine, the automatic combining and automatic separation of the gun head and the consumable tip head on the nucleic acid extraction and detection integrated machine are smoothly realized, thereby further improving the automation level of the nucleic acid extraction and detection integrated machine, with a simple structure and ingenious design, making the nucleic acid extraction experiment more convenient and improving the efficiency of nucleic acid extraction. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only one embodiment of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.
[0022] Figure 1 This is a schematic diagram of the overall structure of the integrated nucleic acid extraction and detection machine provided in the embodiment;
[0023] Figure 2 It is a schematic diagram of the structure of the tip head motion component provided in the embodiment;
[0024] Figure 3 An exploded view of the tip head motion component structure provided in the embodiment;
[0025] Figure 4 It is a schematic cross-sectional structure diagram of the tip head motion assembly provided in the embodiment;
[0026] Figure 5 It is a diagram showing the combination and separation states of the tip head and the tip head motion component provided in the embodiment. DETAILED DESCRIPTION
[0027] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the implementation modes of the present invention are further described in detail below in conjunction with the accompanying drawings. It should be pointed out that the embodiments are only detailed explanations of the present invention and should not be regarded as limitations of the present invention. All features disclosed in the embodiments of the present invention, or all steps in the disclosed methods or processes, except for mutually exclusive features and / or steps, can be combined in any way.
[0028] The tip head motion component of the nucleic acid extraction and detection integrated machine provided in this embodiment is as follows Figure 1-5 As shown, Figure 1 This is a schematic diagram of the overall structure of the nucleic acid extraction and detection integrated machine; Figure 2 This is a schematic diagram of the tip head motion component structure; Figure 3 This is an exploded view of the tip head motion component structure; Figure 4 It is a schematic diagram of the cross-sectional structure of the tip head motion component; Figure 5 This is a diagram showing the state changes of the tip head and the tip head motion component when they are combined and separated.
[0029] The tip in this embodiment is a disposable pipette tip (Zhejiang Boyu Biotechnology Co., Ltd., T200 transparent tip) made of plastic.
[0030] like Figures 1 to 5 The nucleic acid extraction and detection integrated machine 1 needs to absorb, pipette, mix and react multiple reagents through the tip head 2 to achieve nucleic acid extraction, purification and amplification. In order to realize the automatic docking between the nucleic acid extraction and detection integrated machine 1 and the tip head 2, a tip head motion component 3 is set in the nucleic acid extraction and detection integrated machine 1, and the tip head motion component 3 includes a combination motion component 4 and a separation motion component 5; the combination motion component 4 can make the tip head 2 and the gun head 6 combine, so that the gun head 6 drives the tip head 2 to move; the separation motion component 5 prevents the tip head 2 from moving by popping out the fork rod 7, so that the tip head 2 is separated from the gun head 6 that continues to move.
[0031] like Figure 2 , 3 , the combined motion component 4 includes a gun head 6 and a tip head sensing component 8; the tip head sensing component 8 includes a gun head sliding sleeve 9 and a sensor 10, which is used to sense whether the tip head 2 is successfully combined with the gun head. The gun head sliding sleeve 9 can be movably sleeved on the outside of the gun head 6. When the gun head 6 takes the tip head 2, the tip head 2 pushes the gun head sliding sleeve 9 to move upward, and the upper end of the gun head sliding sleeve 9 contacts the sensor 10, triggering the sensor signal, and it is considered that the tip head 2 is successfully combined with the gun head 3. A trigger optical coupler 11 is provided at the upper end of the gun head sliding sleeve 9. When the gun head sliding sleeve 9 moves upward, the trigger optical coupler 11 contacts the sensor 10, and the sensor 10 sends a signal that the tip head 2 is successfully combined with the gun head 3.
[0032] like Figures 2 to 5 , the separation motion assembly 5 ejects the fork rod 7, and under the restriction of the fork rod 7, the tip head 2 stops moving upward, thereby separating the tip head 2 from the gun head 3 which continues to move upward. The fork rod 7 is controlled by the electromagnet 12 to eject or retract. When the electromagnet 12 is energized in one direction, magnetism is generated, so that the fork rod 7 is ejected due to the repulsion of the same poles of the magnet; when the electromagnet 12 is energized in the opposite direction, the opposite magnetism is generated, so that the fork rod 7 is retracted due to the attraction of the opposite poles of the magnet.
[0033] like Figures 2 to 5 The lower end of the gun head sleeve 9 is provided with two notches 13. When the fork rod 13 is extended, it enters the position of the two notches 13 at the lower end of the gun head sleeve 9, so that the tip head 2 stops moving upward and separates from the gun head 3.
[0034] Preferably, the fork rod 7 is in a Y shape. When the fork rod 7 is extended, the upper ends 14 of the Y shape can be inserted into the two notches 13 at the lower end of the gun head slide 9, so that the fork rod 7 can directly contact the upper end of the tip head 2, causing the tip head 2 to detach from the gun head 3 and fall off.
[0035] like Figure 3 The gun head 3 includes a long rod 15 and a ball head 16. The ball head 16 is fixed to the lower end of the long rod 15. When the ball head 16 is embedded in the upper end of the tip head 2, the tip head 2 and the gun head 3 are combined. A first annular ridge 17 is provided on the upper end of the ball head 16, and a second annular ridge 18 is also provided on the long rod 15 above the ball head 16. The first annular ridge 17 and the second annular ridge 18 are used to help fix the tip head 2, so that the tip head 2 and the gun head 3 are more firmly combined. The first annular ridge 17 and the second annular ridge 18 can be designed to match the upper end 19 of the tip head 2, so that the tip head 2 will not fall off after being combined with the gun head 3.
[0036] like Figure 3 The upper end of the long rod 15 is provided with a limiting device 20 for the gun head slide 9, and the limiting device 20 includes a spring 21 and a baffle 22; the upper end of the spring 21 is fixed to the outside of the long rod 15, and is used to prevent the gun head slide 9 from moving upward, and the baffle 22 can abut against the edge 23 of the upper end of the gun head slide 9, preventing the gun head slide 9 from moving downward and separating from the gun head 3. The limiting device 20 can limit the highest and lowest positions of the gun head slide 9, so that the gun head slide 9 is always outside the long rod 15, wrapping the long rod 15, and will not fall. At the same time, the design of the spring 21 can give the gun head slide 9 a certain range of upward movement space. When the tip head 2 is combined with the gun head 3, it can push the gun head slide 9 to move upward, and the spring 21 is compressed; when the tip head 2 is separated from the gun head 3, the gun head slide 9 moves downward due to gravity, is abutted and fixed by the baffle 22, and is in the lowest position, and the spring 21 recovers.
[0037] like Figure 5 As shown, the states of the tip head 2 and the tip head motion component 3 in the nucleic acid extraction and detection integrated machine 1 provided in this embodiment are divided into four states. The first state is the initial state, the combined motion component 4 has not yet been combined with the tip head 2, and the tip head 2 is placed inside the nucleic acid extraction and detection integrated machine 1; the second state is the working state, the combined motion component 4 is combined with the tip head 2 and drives the tip head to complete pipetting and other tasks; the third state is the stop working and separation preparation state, the fork rod 7 of the separation motion component 5 is extended to prevent the tip head 2 from moving upward; the fourth state is the separation state, the fork rod 7 of the separation motion component 5 is retracted, and the tip head 2 is completely separated from the combined motion component 4.
[0038] The above is only a specific implementation method of the utility model, but the protection scope of the utility model is not limited to this. Any changes or substitutions that are not conceived through creative work should be covered within the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope defined in the claims.
Claims
1. A tip head motion component of a nucleic acid extraction and detection machine, characterized in that: It includes a combined motion component and a separated motion component; the combined motion component can combine the tip head with the gun head, so that the gun head drives the tip head to move; the separated motion component prevents the tip head from moving by popping out the shift fork rod, so that the tip head is separated from the gun head that continues to move.
2. The tip head motion assembly according to claim 1, characterized in that: The combined motion component includes a gun head and a tip head sensing component; the tip head sensing component includes a gun head sliding sleeve and a sensor for sensing whether the tip head is successfully combined with the gun head.
3. The tip head motion assembly according to claim 2, characterized in that: The gun head sliding sleeve can be movably sleeved outside the gun head. When the gun head takes the tip head, the tip head pushes the gun head sliding sleeve to move upward, and the upper end of the gun head sliding sleeve contacts the sensor, triggering the sensor signal, and it is considered that the tip head and the gun head are successfully combined.
4. The tip head motion assembly according to claim 3, characterized in that: A trigger optical coupler is provided at the upper end of the gun head sliding sleeve. When the gun head sliding sleeve moves upward, the optical coupler contacts the sensor, and the sensor sends a signal indicating that the tip head is combined with the gun head.
5. The tip head motion assembly according to claim 4, characterized in that: The separation motion assembly pops out the shift fork rod, and under the restriction of the shift fork rod, the tip head stops moving upward, thereby separating the tip head from the gun head that continues to move upward.
6. The tip head motion assembly according to claim 5, characterized in that: The shift fork rod is controlled by an electromagnet to pop out or retract. When the electromagnet is energized in one direction, the shift fork rod pops out, and when the electromagnet is energized in the opposite direction, the shift fork rod is retracted.
7. The tip head motion assembly according to claim 6, characterized in that: The lower end of the gun head sliding sleeve is provided with two notches. When the shift fork rod is extended, it enters the two notches at the lower end of the gun head sliding sleeve, so that the tip head stops moving upward and separates from the gun head.
8. The tip head motion assembly according to claim 7, characterized in that: The gun head comprises a long rod and a ball head, wherein the ball head is fixed to the lower end of the long rod, and when the ball head is embedded in the upper end of the tip head, the tip head and the gun head are combined.
9. The tip head motion assembly according to claim 8, characterized in that: A first annular ridge is provided on the upper end of the ball head, and a second annular ridge is also provided on the long rod above the ball head. The first annular ridge and the second annular ridge are used to help fix the tip head so that the tip head and the gun head are more firmly combined.
10. The tip head motion assembly according to claim 9, characterized in that: A limiting device for a gun head slide is provided at the upper end of the long rod, and the limiting device includes a spring and a baffle; the upper end of the spring is fixed to the outside of the long rod to block the gun head slide from moving upward, and the baffle can resist the edge of the upper end of the gun head slide to prevent the gun head slide from moving downward and separating from the gun head.