Cell climbing slide gripping device

Abstract

The utility model discloses a cell climbing slide gripping device, which comprises a mounting seat, three or four grippers extending downwards are mounted on the mounting seat, the three or four grippers extending downwards are rotationally connected with the mounting seat in a concentric circle manner, each gripper comprises a gripping rod, the bottom end of each gripping rod is connected with a gripping head, and the gripping head is connected with the mounting seat. The grabbing rod and the grabbing head are connected to form a hoe shape, the grabbing head is provided with a cutting edge which is conveniently inserted between the bottom of a cell slide and a culture dish, the upper end of the grabbing rod is connected with a turntable through a connecting rod, and the turntable is rotationally connected with the mounting seat. The cell climbing slide grabbing device is simple in structure and ingenious in arrangement, the cell climbing slide can be easily grabbed in the culture dish, the probability that the cell climbing slide is broken due to misoperation when the cell climbing slide is grabbed is reduced, and therefore test failure caused by breaking of the cell climbing slide is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experimental equipment, in particular to a cell climbing slice grabbing device. Background Art

[0002] Cell culture is a commonly used research method in medical research. In the fields of medicine and other fields, the number of technical indicators for analyzing and observing cell samples is gradually increasing, which requires the frequent use of cell slides for various experiments. Cell slides are glass slides with cells growing on one side. Cell slides are experimental techniques that place cell culture slides flat on the bottom of a well plate to allow cells to grow on the surface of the slide. They are widely used in medical and biological research such as immunocytochemistry, immunofluorescence staining, in situ hybridization, and TUNEL apoptosis detection.

[0003] In the prior art, cell slides are often carried out in 6-well plates, 12-well plates or 24-well plates. Due to the small diameter and relatively deep culture wells, and the fact that the cell slides are tightly attached to the culture plates due to a small amount of residual culture medium, the tension is high, and it is difficult to lift them up with hard objects, and it is easy to cause the slides to break and affect the experiment. At present, people often use ordinary metal tweezers to directly remove cell slides from cell culture dishes or honeycomb cell culture plates. The clamping process is difficult, often causing scratches on the surface of the slides or even causing them to break, destroying the surface cells and seriously affecting the smooth completion of the experiment. For this reason, people have developed a clamping device for cell slides, and some clamping devices for cell slides have also been reported in public literature, such as:

[0004] 1. Chinese Patent: A clamping device for grabbing cell climbing slices, application number: 202121711097.9, application date: 2021.07.26, abstract: The utility model relates to a clamping device for grabbing cell climbing slices, including a lifting module, a plurality of clamps, a clamp driving module, and a cell culture plate, wherein the cell culture plate is provided with a plurality of grooves, and the plurality of clamps and the clamp driving module are installed on the lower side of the lifting module, the lifting module is suitable for driving the clamp driving module and the plurality of clamps to move up and down along a straight line, the clamp driving module is suitable for driving the plurality of clamps to open and close, the groove is suitable for loading and taking cell climbing slices, the plurality of clamps are located on the upper side of the groove, and the plurality of clamps are suitable for grabbing the cell climbing slices. The device greatly reduces the dependence on the operator's experience, and the device can be used simply by understanding it; the work efficiency is greatly improved; the climbing slice is grabbed successfully once, and the damage to the cell structure in the edge area caused by multiple hooking of the cell climbing slices is minimized, while the risk of damaging the cell climbing slices is reduced.

[0005] 2. Chinese Patent: A Cell Slide Grabbing Device, Application Number: 202222317806.6, Application Date: 2022.08.31, Abstract: The utility model relates to a cell slide grabbing device, including a cylinder, the bottom of which is connected to an adsorption disk, a piston ring is slidably installed inside the cylinder, a fixed block is bolted inside the lifting cylinder, and a push rod is slidably connected inside the fixed block, the adsorption disk is located at the top of the climbing sheet, and the lifting cylinder is pulled by an auxiliary ring to drive the piston ring to move upward, so that The air pressure in the adsorption plate becomes smaller and attracts the climbing piece. The floating ring moves downward and drives the two push rods to press down and squeeze. The two clamping rods swing to clamp the climbing piece. The limiting ring is rotated to limit and fix the floating ring. When the clamping piece is released, the clamping of the limiting ring on the floating ring is released. At the same time, the pressure plate is pressed to drive the push rod to move. The push rod drives the pressure block on the surface to squeeze the supporting spring. At the same time, the push rod pushes the sealing piston at the bottom to extend to the bottom of the lifting cylinder, so that atmospheric pressure enters the cylinder, the air pressure remains balanced, the bottom of the adsorption plate loses its adsorption force, and the adsorption on the climbing piece is released.

[0006] Through applied research, it was found that the above-mentioned disclosed cell climbing slice clamp device has a certain clamping force, and the cell climbing slice is tightly attached to the culture plate. When the cell climbing slice clamp is used to clamp the cell climbing slice, the cell climbing slice may be damaged by the cell climbing slice clamp due to improper operation. Summary of the invention

[0007] The purpose of the utility model is to provide a cell slide grabbing device, which has a simple structure and ingenious arrangement, and can easily grab cell slides in the wells of a culture dish or a cell culture plate, thereby reducing the probability of the slides being broken due to improper operation when grabbing the cell slides, thereby avoiding test failure due to the breakage of the cell slides.

[0008] In order to achieve the purpose of the utility model, the technical solution adopted is:

[0009] A cell slide grabbing device comprises a mounting seat, wherein the mounting seat is provided with three or four downwardly extending claws, wherein the three or four downwardly extending claws are rotatably connected to the mounting seat in a concentric circle, wherein the claws comprise a grabbing rod, wherein a grabbing head is connected to the bottom end of the grabbing rod, wherein the grabbing rod and the grabbing head are connected in a hoe shape, wherein the grabbing head is provided with a cutting edge for facilitating insertion between the bottom of the cell slide and a culture dish, wherein when the grabbing claws rotate around the mounting seat, the diameter of the rotating circle of the cutting edge is smaller than the diameter of the cell slide, and the diameter of the rotating circle of the grabbing rod is larger than the diameter of the cell slide, wherein the upper end of the grabbing rod is connected to a turntable via a connecting rod, and the turntable is rotatably connected to the mounting seat.

[0010] Further preferred: the mounting seat is a cylindrical mounting seat, and a pillar is vertically connected to the top center of the mounting seat; a mounting hole is arranged in the middle of the rotating disk, the rotating disk is sleeved on the pillar, one end of the connecting rod is connected to the rotating disk, and the other end of the connecting rod extends toward the outer circle of the mounting seat and is connected to the upper end of the vertically arranged grabbing rod. The rotating disks with three or four downwardly extending claws are sleeved and stacked on the pillar, and the connection position of the connecting rod and the grabbing rod must ensure that all grabbing heads are on the same horizontal plane.

[0011] Further preferred: the pillar is a stud with an external thread, and a locking nut is screwed on the pillar to press and limit the turntable on the top surface of the mounting seat. When the position of the gripper is adjusted and the cell slide is grasped, the locking nut is tightened to press and limit the turntable on the top surface of the mounting seat to prevent the gripper from rotating during the grasping process and causing the cell slide to fall from the grasping head.

[0012] Further preferably, the bottom of the connecting rod is fixedly connected with a support ball head for connecting and supporting the top surface of the mounting seat. Since the turntables of the three or four downwardly extending claws are sleeved and stacked on the pillar, the connecting rod will be at a certain distance from the top surface of the mounting seat. The support ball head is fixedly connected to the bottom of the connecting rod, and the spherical surface of the support ball head contacts the top surface of the mounting seat, which plays a supporting role and is conducive to the rotation of the claw.

[0013] The cell slide grabbing device has a simple structure and is cleverly arranged. Three or four downwardly extending claws are installed on a mounting seat. The three or four downwardly extending claws are connected to the mounting seat in a concentric circle, and the claws are gathered together and lowered along the wall of the culture well. Then, the grabbing heads at the bottom of the claws are inserted under the cell slide. At this time, there is a gap between one side of the cell slide and the bottom of the well of the culture dish or cell culture plate. Then, the claws are rotated around the center of the cell slide until the claws are located at the equal points on the periphery of the cell slide. At this time, the three or four claws lift the cell slide around and separate it from the bottom of the culture dish or cell culture well. The cell slide can be easily grabbed out of the well of the culture dish or cell culture plate by lifting the whole. The probability of the cell slide being broken due to improper operation when grabbing the cell slide is reduced, thereby avoiding the failure of the experiment due to the breakage of the cell slide. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the cell slide grabbing device extending into the culture dish;

[0015] Figure 2 is a schematic diagram of the structure of the gripper;

[0016] Figure 3 This is a schematic diagram of the positional relationship between the cell slide grabbing device extending into the culture dish and the cell slide;

[0017] Figure 4This is a schematic diagram of the position relationship when the cell slide grabbing device extends into the culture dish to grab the cell slide;

[0018] Figure 5 This is a schematic diagram of the cell slide grabbing device grabbing a cell slide out of a culture dish;

[0019] The names corresponding to the numbers in the figure are:

[0020] 1. Culture dish; 2. Gripper; 3. Mounting seat; 4. Locking nut; 5. Pillar; 6. Grabbing head; 7. Cutting edge; 8. Grabbing rod; 9. Connecting rod; 10. Support ball head; 11. Turntable; 12. Mounting hole; 13. Cell slide. DETAILED DESCRIPTION

[0021] In order to make the technical solution and advantages of the present application more clear, the technical solution is clearly and completely described below in conjunction with embodiments. Example

[0022] A cell climbing slice grabbing device, which includes a mounting seat 3, the mounting seat 3 is equipped with three or four downwardly extending claws 2, the three downwardly extending claws 2 are rotatably connected to the mounting seat 3 in a concentric circle, the claws 2 include a grabbing rod 8, the bottom end of the grabbing rod 8 is connected with a grabbing head 6, the grabbing rod 8 and the grabbing head 6 are connected in a hoe shape, the grabbing head 6 is provided with a cutting edge 7 for easy insertion between the bottom of the cell climbing slice 13 and the culture dish 1, the upper end of the grabbing rod 8 is connected to a turntable 11 through a connecting rod 9, and the turntable 11 is rotatably connected to the mounting seat 3.

[0023] The mounting seat 3 is a cylindrical mounting seat, and a pillar 5 is vertically connected to the top center of the mounting seat 3; a mounting hole 12 is arranged in the middle of the rotating disk 11, and the rotating disk 11 is inserted into the pillar 5, one end of the connecting rod 9 is connected to the rotating disk 11, and the other end of the connecting rod 9 extends toward the outer circle of the mounting seat 3 and is connected to the upper end of the vertically arranged grabbing rod 8. The rotating disks 11 of three or four downwardly extending grippers 2 are inserted and stacked on the pillar 5, and the connection position of the connecting rod 9 and the grabbing rod 8 must ensure that all the grabbing heads 6 are on the same horizontal plane.

[0024] The use process of the above-mentioned cell climbing slice grabbing device is as follows: the three grippers 2 are gathered together and lowered along the wall of the culture well, and then the gripping head 6 at the bottom of the gripper 2 is inserted under the cell climbing slice. At this time, there is a gap between one side of the cell climbing slice 13 and the bottom of the culture dish or cell culture plate well. The gripper 2 is then rotated and separated around the center of the cell climbing slice, and turned to the equal division point where the gripper 2 is located on the periphery of the cell climbing slice 13. At this time, the three grippers 2 lift the cell climbing slice 13 around and separate it from the bottom of the culture dish or cell culture well plate. The mounting seat 3 is held by hand to prevent the gripper 2 from rotating, and the cell climbing slice can be easily grabbed out of the culture dish or cell culture plate well by lifting it as a whole. Then, tweezers are used to gently clamp the edge of the cell climbing slice from the side and place it on a glass slide for cell experiments, thereby reducing the probability of the climbing slice being broken due to improper operation when grabbing the cell climbing slice, thereby avoiding the failure of the experiment due to the breakage of the cell climbing slice. Example

[0025] A cell climbing slice grabbing device, which includes a mounting seat 3, the mounting seat 3 is equipped with four downwardly extending claws 2, the four downwardly extending claws 2 are rotatably connected to the mounting seat 3 in a concentric circle, the claws 2 include a grabbing rod 8, the bottom end of the grabbing rod 8 is connected with a grabbing head 6, the grabbing rod 8 and the grabbing head 6 are connected in a hoe shape, the grabbing head 6 is provided with a cutting edge 7 for easy insertion between the bottom of the cell climbing slice 13 and the culture dish 1, the upper end of the grabbing rod 8 is connected to a turntable 11 through a connecting rod 9, and the turntable 11 is rotatably connected to the mounting seat 3.

[0026] The mounting seat 3 is a cylindrical mounting seat, and a pillar 5 is vertically connected to the top center of the mounting seat 3; a mounting hole 12 is arranged in the middle of the rotating disk 11, and the rotating disk 11 is inserted into the pillar 5, one end of the connecting rod 9 is connected to the rotating disk 11, and the other end of the connecting rod 9 extends toward the outer circle of the mounting seat 3 and is connected to the upper end of the vertically arranged grabbing rod 8. The rotating disks 11 of three or four downwardly extending grippers 2 are inserted and stacked on the pillar 5, and the connection position of the connecting rod 9 and the grabbing rod 8 must ensure that all the grabbing heads 6 are on the same horizontal plane.

[0027] The pillar 5 is a stud with an external thread, and a locking nut 4 is screwed on the pillar 5 for pressing and limiting the turntable 11 on the top surface of the mounting seat 3. After adjusting the position of the gripper 2 and grabbing the cell climbing sheet 13, tighten the locking nut 4 to press and limit the turntable 11 on the top surface of the mounting seat 3 to prevent the gripper 2 from rotating during the grabbing process and causing the cell climbing sheet to fall off the grabbing head 6.

[0028] The use process of the above-mentioned cell climbing slice grabbing device is as follows: the four grippers 2 are gathered together and lowered along the wall of the culture well, and then the gripping head 6 at the bottom of the gripper 2 is inserted under the cell climbing slice. At this time, there is a gap between one side of the cell climbing slice 13 and the bottom of the culture dish or cell culture plate well, and then the gripper 2 is rotated and separated around the center of the cell climbing slice, and turned to the equal division point where the gripper 2 is located on the periphery of the cell climbing slice 13. At this time, the four grippers 2 lift the cell climbing slice 13 around and separate it from the bottom of the culture dish or cell culture well plate, tighten the locking nut 4 to press the turntable 11 to the top surface of the mounting seat 3, at this time, the gripper 2 is limited and cannot rotate, and then the gripping device is lifted as a whole to easily grab the cell climbing slice out of the culture dish or cell culture plate well, and then use tweezers to gently clamp the edge of the cell climbing slice from the side and place it on a glass slide for cell experiments, reducing the probability of the climbing slice being broken due to improper operation when grabbing the cell climbing slice, thereby avoiding the failure of the experiment due to the breakage of the cell climbing slice. Example

[0029] The difference from Example 1 or Example 2 is that: the bottom of the connecting rod 9 is fixedly connected with a support ball head 10 for connecting and supporting the top surface of the mounting seat 3. Since the rotating disks 11 of the three or four downwardly extending claws 2 are sleeved and stacked on the pillar 5, the connecting rod 9 will be at a certain distance from the top surface of the mounting seat 3. The support ball head 10 is fixedly connected to the bottom of the connecting rod 9, and the spherical surface of the support ball head 10 contacts the top surface of the mounting seat 3, which plays a supporting role and is conducive to the rotation of the claw 2.

[0030] The above description is not a limitation of the present application, and the present application is not limited to the above examples. Any changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present application should fall within the scope of protection of the present application.

Claims

1. A cell slide grabbing device, characterized in that: The invention comprises a mounting seat (3), wherein the mounting seat (3) is provided with three or four downwardly extending grippers (2), wherein the three or four downwardly extending grippers (2) are rotatably connected to the mounting seat (3) in a concentric circle, wherein the grippers (2) comprise a grabbing rod (8), wherein the bottom end of the grabbing rod (8) is connected to a grabbing head (6), wherein the grabbing rod (8) and the grabbing head (6) are connected in a hoe-like shape, wherein the grabbing head (6) is provided with a cutting edge (7) for facilitating insertion between the bottom of a cell climbing sheet (13) and a culture dish (1), wherein the upper end of the grabbing rod (8) is connected to a rotating disk (11) via a connecting rod (9), and the rotating disk (11) is rotatably connected to the mounting seat (3).

2. The cell slide grabbing device according to claim 1, characterized in that: The mounting seat (3) is a cylindrical mounting seat, and a pillar (5) is vertically connected to the center of the top of the mounting seat (3); a mounting hole (12) is arranged in the middle of the turntable (11), and the turntable (11) is inserted into the pillar (5). One end of the connecting rod (9) is connected to the turntable (11), and the other end of the connecting rod (9) extends toward the outer circle of the mounting seat (3) and is connected to the upper end of the vertically arranged grabbing rod (8).

3. The cell slide grabbing device according to claim 2, characterized in that: The support (5) is a stud with an external thread, and a locking nut (4) is screwed onto the support (5) and is used to press and limit the rotating disk (11) on the top surface of the mounting seat (3).

4. The cell slide grabbing device according to claim 2, characterized in that: The bottom of the connecting rod (9) is fixedly connected to a support ball head (10) for connecting and supporting the top surface of the mounting seat (3).

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