Microfluidic 3D printing blood vessel perfusion device

Abstract

The utility model provides a microfluidic 3D printing blood vessel perfusion device. The microfluidic 3D printing blood vessel perfusion device comprises a base, a cover plate and a threaded straight joint, the base is provided with a first groove and a plurality of through holes, the first groove is used for containing a 3D printing intravascular stent, the through holes are formed in the two opposite side faces of the base, the through holes are communicated with the first groove, the threaded straight-through connectors are connected with the through holes, and the cover plate covers the first groove to form a closed space; and liquid is injected into the 3D printing intravascular stent in the first groove through the threaded straight joint to carry out a perfusion experiment. The 3D printing intravascular stent can be replaced more conveniently, and practicability and leakproofness of a perfusion experiment are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical detection, in particular to a microfluidic 3D printing vascular perfusion device. Background Art

[0002] Cardiovascular stenosis is a common cardiovascular disease, mainly due to the accumulation of plaques in the coronary arteries, which leads to stenosis or blockage of blood vessels, thereby affecting the blood supply to the heart. The incidence of this disease is high, especially in the middle-aged and elderly population. Myocardial infarction is a serious condition among cardiovascular diseases, which is caused by complete blockage of the coronary arteries, resulting in ischemia and necrosis of myocardial tissue. Myocardial infarction is an acute disease that requires emergency treatment. If not treated in time, it can lead to serious consequences or even death. The mortality rate of cardiovascular stenosis and myocardial infarction is relatively high, especially when a large area of ​​myocardial infarction occurs, the risk of death is higher. These diseases have a serious impact on the quality of life and health of patients, and also bring economic burdens and psychological pressures to families and society. It is very important to simulate and stress test the blood flow after cardiovascular stenosis.

[0003] Bio-3D printing is an emerging technology with a wide range of applications in medicine. The vascular stents printed by bio-3D printing can simulate conditions such as vascular wall stenosis, which plays a great role in the treatment of patients. A bio-3D printed vascular stent must undergo multiple tests and pass them before it can be used in the human body, and the perfusion experiment is one of the links to measure whether a bio-3D printed vascular stent is qualified.

[0004] Biological 3D printing products have high requirements for the environment and equipment. The perfusion devices in the existing technology are not suitable for the perfusion of 3D printed blood vessels. If the perfusion path is built according to the 3D printed blood vessels, the technical requirements are relatively high and it will cause certain pollution to the external environment. For the replacement of 3D printed blood vessel stents, it is inconvenient to reconnect the pipeline; if the external perfusion pipeline of the 3D printed blood vessel stent is exposed in the equipment, there is a risk of liquid leakage, which affects the normal operation of the equipment and even pollutes the space environment; if the 3D printed blood vessel stent is integrated with the perfusion equipment, the mechanical and control electrical components cannot be reused, resulting in load waste. Utility Model Content

[0005] In view of the shortcomings of the prior art, the utility model proposes a microfluidic 3D printed vascular perfusion device that can more conveniently replace 3D printed vascular stents, thereby improving the practicality and airtightness of the perfusion experiment.

[0006] The technical solution of the utility model is achieved in this way:

[0007] A microfluidic 3D printed vascular perfusion device, comprising a base, a cover plate and a threaded straight-through connector;

[0008] The base is provided with a first groove for placing the 3D printed blood vessel stent and a plurality of through holes arranged on two opposite sides of the base, the through holes are connected to the first groove, the threaded straight-through connector is connected to the through holes, and the cover plate is covered on the first groove to form a closed space;

[0009] Liquid is injected into the 3D printed vascular stent in the first groove through the threaded straight-through connector to perform a perfusion experiment.

[0010] In a further embodiment, a second groove for accommodating the cover plate is provided on the base, and the first groove is arranged in the middle of the second groove.

[0011] In a further embodiment, the second groove includes a second upper surface, a third upper surface and a fourth upper surface connected in sequence, the second upper surface and the fourth upper surface are vertically arranged relative to the third upper surface, and the second upper surface and the fourth upper surface are respectively recessed in a direction away from one side of the third upper surface.

[0012] In a further embodiment, when the cover plate is located in the second groove, the upper surface of the cover plate is flush with the upper surface of the base.

[0013] In a further embodiment, a third groove and a sealing ring embedded in the third groove are further provided in the second groove, the third groove is arranged around the first groove, and the contour of the sealing ring is adapted to the third groove.

[0014] A further embodiment further comprises a plurality of screws for fixing the cover plate on the base, a plurality of screw holes are correspondingly provided on the cover plate and the second groove, and the screws are passed through the screw holes.

[0015] In a further embodiment, the 3D printed vascular stent is one or more of polycaprolactone material, polylactic acid material, hydrogel material, and inorganic powder material.

[0016] In a further embodiment, a mounting position for fixing the 3D printed vascular stent is provided in the first groove, so that the stent in the first groove is replaceable.

[0017] In a further embodiment, one side of the base is provided with one through hole, and the other side opposite thereto is provided with two through holes.

[0018] Compared with the prior art, the utility model has the following advantages:

[0019] (1) The microfluidic 3D printed blood vessel perfusion device of the present invention can be used for the perfusion of 3D printed blood vessels without causing certain pollution to the environment.

[0020] (2) The microfluidic 3D printed vascular perfusion device of the present invention only needs to contact the outer surface of the integrated device during assembly and use, thereby avoiding contamination of the working area of ​​the device.

[0021] (3) The microfluidic 3D printed vascular perfusion device of the present invention can still ensure that the contents of the device will not leak when the internal pressure of the first groove reaches 1.5 atmospheres. It can ensure that the device will not have the risk of leakage in a vacuum environment or in extreme cases when the outside of the first groove is completely depressurized.

[0022] (4) The 3D printed vascular stent placed in the microfluidic 3D printed vascular perfusion device of the present invention can be replaced back and forth, realizing the multi-measurement function of the device and facilitating use. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0024] Figure 1 This is a schematic diagram of the external structure of the microfluidic 3D printed vascular perfusion device in Example 1 of the present invention;

[0025] Figure 2 Schematic diagram of the internal structure of the microfluidic 3D printed vascular perfusion device in Example 1 of the present invention;

[0026] Figure 3 It is a schematic diagram of the assembly of the microfluidic 3D printed vascular perfusion device in Example 1 of the present invention.

[0027] Figure identification: 1-screw, 2-cover plate, 3-3D printed vascular stent, 4-sealing ring, 5-base, 501-first upper surface, 502-second upper surface, 503-third upper surface, 504-fourth upper surface, 505-fifth upper surface, 6-threaded straight-through connector, 7-first groove, 8-through hole, 9-second groove, 10-third groove. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present utility model. In addition, the terms "first", "second", "third", "fourth", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0030] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] Implementation Method 1

[0032] like Figures 1 to 3 As shown, the embodiment of the utility model discloses a microfluidic 3D printed vascular perfusion device, including a base 5, a cover plate 2 and a threaded straight-through connector 6;

[0033] The base 5 is provided with a first groove 7 for placing the 3D printed blood vessel stent 3 and a plurality of through holes 8 arranged on two opposite sides of the base 5, the through hole 8 is connected to the first groove 7, the threaded straight-through connector 6 is connected to the through hole 8, and the cover plate 2 is covered on the first groove 7 to form a closed space; the cover plate 2 covers the 3D printed blood vessel stent 3 to form a closed space, one end of the threaded straight-through connector 6 is connected to the through hole 8, and the other end is connected to the injection pump, the waste liquid bag, and the liquid storage bag through a silicone tube, and the injection pump injects liquid into the first groove 7 through the silicone tube to perform a perfusion experiment.

[0034] Specifically, Figure 3As shown, the upper surface of the base 5 is composed of a first upper surface 501, a second upper surface 502, a third upper surface 503, a fourth upper surface 504 and a fifth upper surface 505 connected in sequence, the first upper surface 501 is perpendicular to the second upper surface 502, the second upper surface 502 is perpendicular to the third upper surface 503, the third upper surface 503 is perpendicular to the fourth upper surface 504, the fourth upper surface 504 is perpendicular to the fifth upper surface 505, the first upper surface 501 and the fifth upper surface 505 are kept horizontal and at least higher than the third upper surface 503; the second upper surface 502, the third upper surface 503 and the fourth upper surface 504 form a second groove 9 for accommodating the cover plate 2, and the first groove 7 is arranged in the middle of the second groove 9. When the cover plate 2 is arranged in the second groove 9, the first groove 7 and the internal 3D printed blood vessel stent 3 are both located inside the base 5, which effectively improves the precision of the perfusion experiment.

[0035] In a further implementation method, the second upper surface 502 and the fourth upper surface 504 are respectively recessed in a direction away from the third upper surface 503, so that the shape of the cover plate 2 is adapted to the second groove 9, so that the cover plate 2 can better cover the first groove 7, so that the 3D printed blood vessel is in a closed space, and the perfusion experiment can operate normally under different air pressures.

[0036] like Figure 2 As shown, further, when the cover plate 2 is located in the second groove 9, the upper surface of the cover plate 2 is flush with the upper surface of the base 5, that is, the upper surface of the cover plate 2 is level with the fourth upper surface 504. The cover plate 2 can cover the first groove 7 without leaving a gap, thereby increasing the airtightness of the device.

[0037] In a further embodiment, a third groove 10 and a sealing ring 4 embedded in the third groove 10 are further provided in the second groove 9, the third groove 10 is arranged around the first groove 7, and the contour of the sealing ring 4 is adapted to the third groove 10. Specifically, the third groove 10 is a strip groove connected end to end, the diameter of the sealing ring 4 is slightly larger than the depth of the third groove 10, and when the cover plate 2 is arranged in the second groove 9, the sealing ring 4 can better keep the first groove 7 in a closed state. In this embodiment, the preferred sealing ring 4 is a polygon.

[0038] A further embodiment further includes a plurality of screws 1 for fixing the cover plate 2 on the base 5, and a plurality of screw holes are correspondingly provided on the cover plate 2 and the second groove 9, and the screws 1 are passed through the screw holes. Thus, the cover plate 2 and the base 5 are fixed as one, so that the cover plate 2 is fixed on the base 5 more stably.

[0039] In a further embodiment, the 3D printed vascular stent 3 is one or more of polycaprolactone material, polylactic acid material, hydrogel material, and inorganic powder material.

[0040] In an optional embodiment, a mounting position for fixing the 3D printed vascular stent 3 is provided in the first groove 7, so that the first groove 7 can be replaced. It is understandable that the structure of the mounting position is not specifically limited, as long as the 3D printed vascular stent 3 is ensured not to be displaced in the first groove 7. The structure of the mounting position can be a protrusion at the bottom, a buckle, etc. The reusability of the device is effectively improved, and the 3D printed vascular stent 3 can be replaced in the first groove 7, reducing the cost.

[0041] In a further embodiment, one side of the base 5 is provided with a through hole 8, and the other side opposite thereto is provided with two through holes 8. This enables the device to meet the perfusion experiment requirements of the Y-shaped 3D printed vascular stent 3.

[0042] Implementation Method 2

[0043] The present embodiment also proposes a method for using a microfluidic 3D printed vascular perfusion device, comprising the following steps: S1: installing the device, first placing a 3D printed vascular stent 3 in the first groove 7, with the blood vessel direction of the 3D printed vascular stent 3 facing the threaded straight connector 6, then placing a sealing ring 4 in the third groove 10, then covering the cover plate 2 in the second groove 9, screwing on the screw 1 and the threaded straight connector 6, and finally connecting the threaded straight connector 6 to the injection pump, the liquid storage bag, and the waste liquid bag through a silicone tube, and the device is installed;

[0044] S2: The syringe pump injects liquid to start perfusion.

[0045] The beneficial effects of the utility model are:

[0046] (1) The microfluidic 3D printed blood vessel perfusion device of the present invention can be used for the perfusion of 3D printed blood vessels without causing certain pollution to the environment.

[0047] (2) The microfluidic 3D printed vascular perfusion device of the present invention only needs to contact the outer surface of the integrated device during assembly and use, thereby avoiding contamination of the working area of ​​the device.

[0048] (3) The microfluidic 3D printed vascular perfusion device of the present invention can still ensure that the contents of the device will not leak when the internal pressure of the first groove reaches 1.5 atmospheres. It can ensure that the device will not have the risk of leakage in a vacuum environment or in extreme cases when the outside of the first groove is completely depressurized.

[0049] (4) The 3D printed vascular stent 3 placed in the microfluidic 3D printed vascular perfusion device of the present invention can be replaced back and forth, realizing the multi-measurement function of the device and facilitating use.

[0050] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microfluidic 3D printed vascular perfusion device, characterized in that: It comprises a base (5), a cover plate (2) and a threaded straight-through connector (6); The base (5) is provided with a first groove (7) for placing the 3D printed blood vessel stent (3) and a plurality of through holes (8) arranged on two opposite sides of the base (5), the through holes (8) are connected to the first groove (7), the threaded straight joint (6) is connected to the through holes (8), and the cover plate (2) is arranged to cover the first groove (7) to form a closed space; Liquid is injected into the 3D printed blood vessel stent (3) in the first groove (7) through the threaded straight-through connector (6) to perform a perfusion experiment.

2. A microfluidic 3D printed vascular perfusion device according to claim 1, characterized in that: The base (5) is provided with a second groove (9) for accommodating the cover plate (2), and the first groove (7) is arranged in the middle of the second groove (9).

3. A microfluidic 3D printed vascular perfusion device according to claim 2, characterized in that: The second groove (9) comprises a second upper surface (502), a third upper surface (503) and a fourth upper surface (504) which are connected in sequence, the second upper surface (502) and the fourth upper surface (504) are arranged vertically relative to the third upper surface (503), and the second upper surface (502) and the fourth upper surface (504) are respectively recessed in a direction away from the third upper surface (503).

4. A microfluidic 3D printed vascular perfusion device according to claim 3, characterized in that: When the cover plate (2) is located in the second groove (9), the upper surface of the cover plate (2) is flush with the upper surface of the base (5).

5. A microfluidic 3D printed vascular perfusion device according to claim 2, characterized in that: A third groove (10) and a sealing ring (4) embedded in the third groove (10) are also provided in the second groove (9); the third groove (10) is arranged around the first groove (7); and the contour of the sealing ring (4) is adapted to the third groove (10).

6. A microfluidic 3D printed vascular perfusion device according to claim 2, characterized in that: It also includes a plurality of screws (1) for fixing the cover plate (2) on the base (5); a plurality of screw holes are correspondingly arranged on the cover plate (2) and the second groove (9); and the screws (1) are inserted into the screw holes.

7. A microfluidic 3D printed vascular perfusion device according to claim 1, characterized in that: The 3D printed vascular stent (3) is one or more of polycaprolactone material, polylactic acid material, hydrogel material, and inorganic powder material.

8. The microfluidic 3D printed vascular perfusion device according to claim 1, characterized in that: A mounting position for fixing the 3D printed blood vessel stent (3) is provided in the first groove (7), so that the 3D printed blood vessel stent (3) can be replaced in the first groove (7).

9. The microfluidic 3D printed vascular perfusion device according to claim 1, characterized in that: One side of the base (5) is provided with one through hole (8), and the other side opposite thereto is provided with two through holes (8).

Images