Separation and purification device for garlic extracting solution

Abstract

The utility model discloses a garlic extract liquid separation purification device, including raw material tank, pH regulation mechanism, garlic polysaccharide extraction mechanism and alliinase extraction mechanism, the pH regulation mechanism includes first pH regulation device, centrifuge and second pH regulation device that are connected through pipeline in proper order, the first pH regulation device is connected with raw material tank through pipeline, the centrifuge is connected with the second pH regulation device through pipeline, and the alliinase extraction mechanism is connected with the second pH regulation device through pipeline. The garlic polysaccharide extraction mechanism comprises a resin column, an ultrafiltration concentration device, a water purification device and a dialysis device, an ultrafiltration permeate outlet of the ultrafiltration concentration device is sequentially connected with the water purification device and the dialysis device through pipelines, and a concentrated solution outlet of the ultrafiltration concentration device is connected with the dialysis device through a pipeline; a liquid outlet of the dialysis device is sequentially connected with an airflow dryer and a garlic polysaccharide product tank through pipelines, and the allinase extraction mechanism comprises a dissolving device, an ultrafiltration device and an allinase product tank which are sequentially connected through pipelines. The garlic polysaccharide and alliinase separation and purification device is simple in process, good in impurity removal effect, capable of effectively separating and purifying garlic polysaccharide and alliinase and high in product purity.

Description

Technical Field

[0001] The utility model belongs to the technical field of polysaccharide extraction, and in particular relates to a separation and purification device for garlic extract. Background Art

[0002] Garlic polysaccharide is a polysaccharide extracted from garlic, which has good biological activity, including antibacterial, antiviral, antitumor and other effects. In recent years, with the development of science and technology, the research and utilization of natural medicines have received more and more attention. As a natural medicine with broad application prospects, the research and development of garlic polysaccharide has become a topic of widespread concern. However, garlic polysaccharide mainly exists in the cell wall of garlic, and its extraction process is complicated, the purity is low, and there are many impurities. These problems seriously affect the quality of garlic polysaccharide. Therefore, how to effectively extract garlic polysaccharide and improve its yield and purity has become a problem to be solved urgently.

[0003] On the other hand, alliinase is an important enzyme protein in garlic, belonging to the thioetherase family. It is mainly present in the cytoplasm of garlic, has strong amino acid hydrolysis activity, and can catalyze the hydrolysis reaction of amino acids and polypeptides. Alliinase not only plays an important role in garlic, but is also widely used in industrial production and biological research. In the pharmaceutical field, alliinase is widely used in some drugs and health products. Its main pharmaceutical uses include: antihypertensive effect: alliinase can promote the synthesis of sulfided amino acids and increase the synthesis of nitric oxide, thereby dilating blood vessels and lowering blood pressure. It has a significant antihypertensive effect and can be used to treat hypertension and cardiovascular and cerebrovascular diseases. Antithrombotic effect: alliinase can reduce plasma viscosity, inhibit platelet aggregation and enhance vascular elasticity. It has an antithrombotic effect and helps prevent and treat cardiovascular and cerebrovascular diseases. Antibacterial and anti-inflammatory effect: alliinase has certain antibacterial and anti-inflammatory effects, which can help the body resist bacterial and viral infections and enhance immunity. Assisting in lowering blood lipids: alliinase can promote fat metabolism, reduce blood lipid content, help regulate blood lipid levels, and prevent and treat hyperlipidemia. Due to the complex tissue structure of garlic, alliinase exists in cells and needs to break the cell walls to be released, making extraction difficult. In addition, since alliinase has poor solubility in general organic solvents and water, it is difficult to dissolve it from cells during the extraction process. Summary of the invention

[0004] In order to solve the deficiencies of the prior art, the purpose of the utility model is to provide a separation and purification device for garlic extract, which has a simple process and good impurity removal effect, can effectively separate and purify garlic polysaccharides and alliinase, and the product purity is high.

[0005] In order to achieve the above objectives, the utility model adopts the following technical solutions:

[0006] A device for separating and purifying garlic extract comprises a raw material tank, a pH adjusting mechanism, a garlic polysaccharide extraction mechanism and an alliinase extraction mechanism. The pH adjusting mechanism comprises a first pH adjusting device, a centrifuge and a second pH adjusting device which are sequentially connected through pipelines. The first pH adjusting device is connected to the raw material tank through a pipeline. The garlic polysaccharide extraction mechanism comprises a resin column, an ultrafiltration concentration device, a pure water device and a dialysis device. The second pH adjusting device is sequentially connected to the resin column and the ultrafiltration concentration device through a pipeline. The ultrafiltration permeate outlet of the ultrafiltration concentration device is sequentially connected to the pure water device and the dialysis device through a pipeline. The concentrated liquid outlet of the ultrafiltration concentration device is connected to the dialysis device through a pipeline. The liquid outlet of the dialysis device is sequentially connected to an air flow dryer and a garlic polysaccharide product tank through a pipeline. The alliinase extraction mechanism comprises a dissolving device, an ultrafiltration device and an alliinase product tank which are sequentially connected through pipelines. The dissolving device is connected to the sediment outlet of the centrifuge through a pipeline.

[0007] Preferably, the first pH adjusting device is connected to the acid solution storage tank through a pipeline, and the second pH adjusting device is connected to the alkali solution storage tank through a pipeline.

[0008] Preferably, the aforementioned resin column adopts D101 macroporous adsorption resin column.

[0009] Preferably, the ultrafiltration concentration device is provided with a ceramic ultrafiltration membrane element, the ceramic ultrafiltration membrane element has a diameter of 52 mm, a length of 1.5 m, includes 61 channels, the channel diameter is 4 mm, and the filtration area of ​​a single membrane tube is 1.29 m 2 .

[0010] Preferably, the molecular weight cut-off of the aforementioned ceramic ultrafiltration membrane element is 1~30KD.

[0011] Preferably, a freeze drying device is provided between the aforementioned ultrafiltration device and the alliinase product tank.

[0012] Preferably, the aforementioned pure water device adopts a reverse osmosis membrane device, and its retentate outlet is connected to a sewage treatment device through a pipeline.

[0013] The utility model is beneficial in that: the process is simple, the impurity removal effect is good, garlic polysaccharide and alliinase can be effectively separated and purified, the product purity is high, and the purified garlic polysaccharide solution has high light transmittance; garlic polysaccharide can be effectively separated from proteins such as alliinase by pH adjustment, and the activity of alliinase can be better maintained; non-water-soluble impurities can be removed by adsorption through a macroporous adsorption resin column, and small molecular impurities such as pigments and minerals can be further removed through ultrafiltration concentration treatment, and the purity of garlic polysaccharide can be further improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the utility model.

[0015] The meanings of the reference numerals in the figure are as follows: 1. raw material tank, 2. resin column, 3. ultrafiltration concentration device, 4. first pH adjustment device, 5. centrifuge, 6. second pH adjustment device, 7. pure water device, 8. dialysis device, 9. air flow dryer, 10. garlic polysaccharide product tank, 11. acid storage tank, 12. alkali storage tank, 13. sewage treatment device, 14. dissolution device, 15. ultrafiltration device, 16. alliinase product tank, 17. freeze drying device. DETAILED DESCRIPTION

[0016] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0017] See also Figure 1 The utility model discloses a separation and purification device for garlic extract, which comprises a raw material tank 1, a pH adjustment mechanism, a garlic polysaccharide extraction mechanism and an alliinase extraction mechanism.

[0018] The pH adjusting mechanism comprises a first pH adjusting device 4, a centrifuge 5 and a second pH adjusting device 6 which are sequentially connected through pipelines. The inlet of the first pH adjusting device 4 is connected to a raw material tank 1 and an acid storage tank 11 through a pipeline, and the second pH adjusting device 6 is connected to an alkali storage tank 12 through a pipeline. Acid is added to the garlic extract to adjust the pH to pH 4.0, so that garlic polysaccharide can be effectively separated from proteins such as alliinase. After centrifugal precipitation, the precipitate is used to extract alliinase. The supernatant is neutralized to neutrality with alkali solution, and after neutralization, it is passed through a macroporous resin column 2. After the sample is loaded, the effluent is collected to obtain a decolorized and deodorized garlic polysaccharide solution.

[0019] The garlic polysaccharide extraction mechanism includes a resin column 2, an ultrafiltration concentration device 3, a pure water device 7 and a dialysis device 8. The second pH adjustment device 6 is connected to the resin column 2 and the ultrafiltration concentration device 3 in sequence through a pipeline. The ultrafiltration permeate outlet of the ultrafiltration concentration device 3 is connected to the pure water device 7 and the dialysis device 8 in sequence through a pipeline. The concentrated liquid outlet of the ultrafiltration concentration device 3 is connected to the dialysis device 8 through a pipeline. The liquid outlet of the dialysis device 8 is connected to the air flow dryer 9 and the garlic polysaccharide product tank 10 in sequence through a pipeline.

[0020] The resin column 2 uses a D101 macroporous adsorption resin column 2, which can remove water-insoluble impurities by adsorption, thereby improving the purity of garlic polysaccharides. The ultrafiltration concentration device 3 is provided with a ceramic ultrafiltration membrane element, which has a diameter of 52 mm and a length of 1.5 m, including 61 channels with a channel diameter of 4 mm. The filtration area of ​​a single membrane tube is 1.29 m 2. The molecular weight cutoff of the ceramic ultrafiltration membrane element is 1~30KD. The operating pressure of the ultrafiltration concentration device 3 is 0.6~1Mpa, the membrane surface flow rate is 4m / s, and the concentration multiple is 20~30 times. The garlic extract can be ultrafiltered through a small molecular weight ceramic ultrafiltration membrane to remove impurities such as pigments, minerals, and small molecules. The pure water device 7 is used to filter the ultrafiltration permeate produced by the ultrafiltration concentration device 3 to obtain deionized water, and then the concentrate of the ultrafiltration concentration device 3 is dialyzed with deionized water to remove more impurities and improve the purity of garlic polysaccharides.

[0021] The alliinase extraction mechanism includes a dissolving device 14, an ultrafiltration device 15 and an alliinase product tank 16 which are sequentially connected by pipelines, and the dissolving device 14 is connected to the sediment outlet of the centrifuge 5 by a pipeline. A freeze drying device 17 is arranged between the ultrafiltration device 15 and the alliinase product tank 16. The ultrafiltration device 14 uses an ultrafiltration membrane with a molecular weight cutoff of 7000 to effectively remove small molecular impurities.

[0022] Embodiment: (1) pH adjustment: the garlic extract in the raw material tank 1 is sent to the first pH adjustment device 4, hydrochloric acid is added dropwise to the garlic extract, and the mixture is rapidly stirred until the pH reaches 4.0, and the mixture is sent to the centrifuge 5 for centrifugal precipitation, and the supernatant is sent to the second pH adjustment device 6, and neutralized to neutrality with sodium hydroxide;

[0023] (2) Separation: The pH-adjusted garlic extract was sent to the macroporous resin column 2, the flow rate was controlled to be 6 mL / min, and the effluent was collected to obtain the garlic polysaccharide solution;

[0024] (3) Ultrafiltration and concentration: The garlic polysaccharide solution is sent to the ultrafiltration and concentration device 3 for ultrafiltration and concentration treatment, with an operating pressure of 0.6-1 MPa, a membrane surface flow rate of 4 m / s, and a concentration multiple of 20-30 times;

[0025] (4) Dialysis: The ultrafiltration permeate produced by the ultrafiltration concentration device 3 is sent to the pure water device 7 for filtration to obtain deionized water, and then the deionized water and the concentrate of the ultrafiltration concentration device 3 are sent to the dialysis device 8 for dialysis to further remove impurities;

[0026] (5) Drying: The dialyzed garlic polysaccharide concentrate is sent to an air flow dryer 9 for drying to obtain garlic polysaccharide powder, which is then stored in a garlic polysaccharide product tank 10;

[0027] (6) Dissolving: The precipitate from the centrifuge in step (1) is sent to a dissolving device and quickly dissolved with a phosphate buffer solution having a pH of 6.23 to obtain a crude alliinase;

[0028] (7) Filtration and drying: The crude alliinase is sent to an ultrafiltration device. After filtration, the retentate is freeze-dried to obtain the alliinase product.

[0029] Product performance testing

[0030] 1. The transmittance and precipitation rate of the garlic polysaccharide obtained in the example were tested, wherein the transmittance determination step was: taking distilled water as a blank, taking a sample and measuring the transmittance at a wavelength of 660 nm to detect the clarification effect. The test results are shown in Table 1.

[0031] Table 1 Transmittance and precipitation rate of garlic polysaccharide

[0032]

[0033] It can be seen from Table 1 that pH adjustment can effectively improve the clarification effect of garlic polysaccharide, the resin column has a good impurity removal effect, and the transmittance is significantly improved.

[0034] 2. The activity recovery rate and specific activity of the alliinase obtained in the embodiment were detected, wherein the enzyme activity determination step was: taking alliin as a substrate, taking 1 ml of the above-mentioned alliin concentrate, adding 1 ml of enzyme solution, reacting in a 25 ml stoppered test tube for 3 min, immediately adding 2 ml of 10% trichloroacetic acid to terminate the reaction, adding 1 ml of 12,4-dinitrophenylhydrazine, keeping warm at 25°C for 5 min, then adding 5 ml of 2.5 mol / L NaOH, reacting for 10 min, and then colorimetric analysis at 520 nm (with the inactivated enzyme, substrate, 2,4-dinitrophenylhydrazine, and NaOH reaction system as blank), and the detection results are shown in Table 2.

[0035] Table 2 Activity recovery rate and specific activity of alliinase

[0036]

[0037] As shown in Table 2, after the pH value was adjusted to 4.0 before passing through the resin column, and then the precipitate was quickly dissolved in a phosphate buffer solution with a pH of 6.23, the activity recovery rate of alliinase could reach 65%, and the specific activity was 15.624.

[0038] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the above embodiments do not limit the utility model in any form, and any technical solution obtained by equivalent replacement or equivalent transformation falls within the protection scope of the utility model.

Claims

1. A separation and purification device for garlic extract, characterized in that: The invention comprises a raw material tank (1), a pH adjustment mechanism, a garlic polysaccharide extraction mechanism and an alliinase extraction mechanism, wherein the pH adjustment mechanism comprises a first pH adjustment device (4), a centrifuge (5) and a second pH adjustment device (6) which are sequentially connected via pipelines, the first pH adjustment device (4) is connected to the raw material tank (1) via a pipeline, the garlic polysaccharide extraction mechanism comprises a resin column (2), an ultrafiltration concentration device (3), a pure water device (7) and a dialysis device (8), and the second pH adjustment device (6) is sequentially connected to the resin column (2) and the ultrafiltration concentration device (3) via a pipeline. The ultrafiltration permeate outlet of the ultrafiltration concentration device (3) is connected to the pure water device (7) and the dialysis device (8) in sequence through pipelines; the concentrate outlet of the ultrafiltration concentration device (3) is connected to the dialysis device (8) through pipelines; the liquid outlet of the dialysis device (8) is connected to the air flow dryer (9) and the garlic polysaccharide product tank (10) in sequence through pipelines; the alliinase extraction mechanism comprises a dissolution device (14), an ultrafiltration device (15) and an alliinase product tank (16) connected in sequence through pipelines; the dissolution device (14) is connected to the sediment outlet of the centrifuge (5) through a pipeline.

2. The separation and purification device for garlic extract according to claim 1, characterized in that: The first pH adjusting device (4) is connected to the acid solution storage tank (11) through a pipeline, and the second pH adjusting device (6) is connected to the alkali solution storage tank (12) through a pipeline.

3. The separation and purification device for garlic extract according to claim 1, characterized in that: The resin column (2) adopts D101 macroporous adsorption resin column.

4. The separation and purification device for garlic extract according to claim 1, characterized in that: The ultrafiltration concentration device (3) is provided with a ceramic ultrafiltration membrane element, the ceramic ultrafiltration membrane element has a diameter of 52 mm, a length of 1.5 m, includes 61 channels, the channel diameter is 4 mm, and the filtration area of ​​a single membrane tube is 1.29 m 2 .

5. The separation and purification device for garlic extract according to claim 4, characterized in that: The molecular weight cut-off of the ceramic ultrafiltration membrane element is 1-30KD.

6. The device for separating and purifying a garlic extract according to claim 1, characterized in that: A freeze drying device (17) is provided between the ultrafiltration device and the alliinase product tank.

7. The device for separating and purifying garlic extract according to claim 1, characterized in that: The pure water device (7) adopts a reverse osmosis membrane device, and its retentate outlet is connected to a sewage treatment device (13) through a pipeline.

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