POCT blood cell counter

Abstract

The application discloses a POCT blood cell counter, the POCT blood cell counter receives and transmits a kit through a pipetting mechanism, and the kit is used for storing a sample and / or a reagent; a blood cell counting mechanism of the POCT blood cell counter is arranged in a shell, a main body of the blood cell counting mechanism is fixedly mounted in the shell, a suction and discharge assembly is connected with the main body through a pipeline, and a first driving part is used for driving the suction and discharge assembly to move relative to a kit so as to complete liquid suction and / or liquid discharge operation. Through the mode, the first driving piece only drives the suction and discharge assembly to move relative to the kit to complete liquid suction and discharge operation, the main body fixed in the shell can perform impedance counting on samples in the kit through the liquid suction and discharge operation of the suction and discharge assembly, and the kit can realize rapid detection without independently arranging a counting mechanism; and the manufacturing cost of the kit can be reduced.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a POCT blood cell counter. Background Art

[0002] At present, when POCT blood cell counters perform blood cell testing through a test kit, the microporous sheet used for counting and testing is usually integrated into the test kit, and a new test kit is replaced for testing after the sample test is completed. However, due to the high cost of the microporous sheet, integrating the microporous sheet into the test kit will increase the manufacturing cost of the test kit, which is not conducive to the further development of POCT blood cell counters. Utility Model Content

[0003] In order to solve the technical problem of high manufacturing cost of the test kit in the prior art, the present application provides a POCT blood cell counter.

[0004] In order to solve the technical problems existing in the prior art, the present application provides a POCT blood cell counter, comprising a shell, a transfer mechanism, a first driving member and a blood cell counting mechanism; the above-mentioned transfer mechanism is arranged in the above-mentioned shell, and the above-mentioned transfer mechanism is used to receive and transmit the test kit, and the above-mentioned test kit is used to store samples and / or reagents; the above-mentioned blood cell counting mechanism is arranged in the above-mentioned shell, and the above-mentioned blood cell counting mechanism includes a main body and a suction and discharge component; the above-mentioned main body is fixedly installed in the above-mentioned shell; the above-mentioned suction and discharge component is connected to the above-mentioned main body through a pipeline; the above-mentioned first driving member is used to drive the above-mentioned suction and discharge component to move relative to the above-mentioned test kit to complete the suction and / or discharge operation.

[0005] Optionally, the above-mentioned suction and discharge assembly includes a liquid suction component, a liquid discharge component and a seat body, the above-mentioned liquid suction component and liquid discharge component are arranged on the above-mentioned seat body, and the above-mentioned seat body is connected to the above-mentioned first driving component; the above-mentioned main body also includes a liquid suction connector and a liquid discharge connector, the above-mentioned liquid suction component is connected to the above-mentioned liquid suction connector through a pipeline, and the above-mentioned liquid discharge component is connected to the above-mentioned liquid discharge connector through a pipeline.

[0006] Optionally, the main body is provided with a front cavity and a rear cavity, the blood cell counting mechanism further comprises a microporous sheet, the microporous sheet is provided with micropores, the front cavity and the rear cavity are connected through the micropores; the main body further comprises a quick-release part, the microporous sheet is detachably connected to the front cavity through the quick-release part; or, the main body as a whole is detachably installed in the shell.

[0007] Optionally, the blood cell counting mechanism further comprises a first shielding member, which is disposed outside the main body for electromagnetic shielding, and the first shielding member is provided with a through hole, and the pipeline passes through the through hole to connect the suction and discharge assembly and the main body.

[0008] Optionally, the shell includes a side panel, which serves as a component of the first shielding component. The side panel also includes an opening and closing door, which is used to shield or reveal the accommodating space formed by the first shielding component in different states so as to disassemble the entire body and / or the quick-release parts.

[0009] Optionally, the main body is provided with a front cavity and a rear cavity, the blood cell counting mechanism further comprises a microporous sheet, the microporous sheet is provided with micropores, the front cavity and the rear cavity are connected through the micropores; the suction and discharge assembly comprises a liquid suction piece and a liquid discharge piece; the liquid suction piece is connected with the front cavity, and the liquid discharge piece is connected with the rear cavity; the POCT blood cell counter further comprises a pressure building mechanism, the pressure building mechanism is connected with the rear cavity, the pressure building mechanism is used to provide negative pressure to the rear cavity, so that the test sample of the test kit enters the front cavity through the liquid suction piece under the action of negative pressure, and enters the rear cavity through the micropores; the pressure building mechanism is also used to provide positive pressure to the rear cavity, so that the test sample in the rear cavity is discharged through the liquid discharge piece under the action of positive pressure.

[0010] Optionally, the POCT blood cell counter further comprises a pipetting mechanism, which is disposed in the housing and is used for mixing the reagent and the sample in the first liquid pool of the reagent kit so that the first liquid pool is loaded with the test sample.

[0011] Optionally, the transfer mechanism includes a movable guide rail, a tray and a second driving member, the tray is slidably connected to the movable guide rail, the second driving member is connected to the tray, and the second driving member is used to drive the tray to slide along the movable guide rail; the test kit includes a plurality of liquid pools arranged linearly, the tray is used to carry the test kit, the second driving member is used to drive the test kit to move along the extension direction of the movable guide rail, and the pipetting mechanism is used to perform pipetting operations on the plurality of liquid pools to mix the reagents and the samples and obtain the test samples.

[0012] Optionally, the transfer mechanism further includes a second shielding member, wherein the second shielding member is disposed on the second driving member, and the second shielding member is used to shield the second driving member from electromagnetic signals.

[0013] Optionally, the above-mentioned pipetting mechanism includes a third driving member, an adapter head and a third shielding member. The above-mentioned third driving member is connected to the above-mentioned adapter head, and the above-mentioned third driving member is used to drive the above-mentioned adapter head to move in a vertical direction. The above-mentioned adapter head is used to plug in the pipette head and perform pipetting operations through the above-mentioned pipette head. The above-mentioned third shielding member is covered on the above-mentioned third driving member, and the above-mentioned third shielding member is used to shield the electromagnetic signals of the above-mentioned third driving member.

[0014] Compared with the prior art, the POCT blood cell counter of the present application receives and transmits the test kit through a pipetting mechanism, and the test kit is used to store samples and / or reagents; the blood cell counting mechanism of the POCT blood cell counter is arranged in a shell, the main body of the blood cell counting mechanism is fixedly installed in the shell, the suction and discharge assembly is connected to the main body through a pipeline, and the first driving member is used to drive the suction and discharge assembly to move relative to the test kit to complete the suction and / or discharge operation. In the above manner, the first driving member only drives the suction and discharge assembly to move relative to the test kit to complete the suction and discharge operation, and the main body fixed in the shell can perform impedance counting on the sample in the test kit through the suction and discharge operation of the suction and discharge assembly, and the test kit can also achieve rapid detection without the need for a separate counting mechanism, which is conducive to reducing the manufacturing cost of the test kit. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 It is a structural schematic diagram of the first embodiment of the POCT blood cell counter provided by the present application;

[0017] Figure 2 yes Figure 1 A schematic structural diagram of a first embodiment of a blood cell counting mechanism;

[0018] Figure 3 yes Figure 1 A schematic structural diagram of a second embodiment of a blood cell counting mechanism;

[0019] Figure 4 It is a structural schematic diagram of an embodiment of a pressure building mechanism provided by the present application;

[0020] Figure 5 It is a structural schematic diagram of the second embodiment of the POCT blood cell counter provided by the present application.

[0021] In the figure, there is shown a POCT blood cell counter A; a blood cell counting mechanism 1; a seat; a main body 11; a front cavity 111; a rear cavity 112; a quick-release part 113; a liquid suction connector 114; a liquid discharge connector 115; a microporous sheet 12; a first electrode 13; a second electrode 14; a suction and discharge assembly 16; a liquid suction part 161; a liquid discharge part 162; a switch valve 18; a first driving part 15; a fourth shielding part 151; a first shielding part 19; a pressure building mechanism 2; a pressure pump 21; a first valve 22; a second valve 23; a three-way connector 24; a transfer mechanism 3; a moving guide rail 31; a tray 32; a second driving part 33; a second shielding part 34; a liquid transfer mechanism 4; a third driving part 41; an adapter head 42; a third shielding part 43; a shell 5; a side panel 51; an opening and closing door 52; and a reagent kit B. DETAILED DESCRIPTION

[0022] The present application is further described in detail below in conjunction with the accompanying drawings and examples. It is particularly noted that the following examples are only used to illustrate the present application, but are not intended to limit the scope of the present application. Similarly, the following examples are only some embodiments of the present application rather than all embodiments, and all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present application.

[0023] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0024] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "set", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or connected through an intermediate medium. For ordinary technicians in this field, if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of this application, the directional indications are only used to explain the relative position relationship, movement, etc. between the components in a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0025] See also Figure 1-Figure 3 , Figure 1 is a schematic structural diagram of the first embodiment of the POCT blood cell counter provided by the present application, Figure 2 yes Figure 1A schematic structural diagram of a first embodiment of a blood cell counting mechanism, Figure 3 yes Figure 1 A schematic diagram of the structure of the second embodiment of the blood cell counting mechanism. Figure 1-Figure 3 As shown, in the embodiment of the present application, the POCT blood cell counter A includes a housing 5, a transfer mechanism 3, a first driving member 15 and a blood cell counting mechanism 1.

[0026] Specifically, the housing 5 is used to shield the transfer mechanism 3, the first driving member 15 and the blood cell counting mechanism 1, so that each work process of the POCT blood cell counter A can be executed in a closed environment, reducing external interference and sample leakage risks. The transfer mechanism 3 is arranged in the housing 5, and the transfer mechanism 3 is used to receive and transmit the test kit B, and the test kit B is used to store samples and / or reagents. The blood cell counting mechanism 1 is arranged in the housing 5, and the blood cell counting mechanism 1 includes a main body 11 and an aspiration and discharge assembly 16. The main body 11 is fixedly installed in the housing 5, and the aspiration and discharge assembly 16 is connected to the main body 11 through a pipeline. The first driving member 15 is arranged in the housing 5, and the first driving member 15 is used to drive the aspiration and discharge assembly 16 to move, so that the aspiration and discharge assembly 16 can move relative to the test kit B, and the aspiration and discharge assembly 16 is used to perform aspiration and / or discharge operations on the test kit B. Among them, the samples and / or reagents in the test kit B can be mixed and reacted under external operation, so that the test kit B stores test samples that can be used for the blood cell counting mechanism 1 to test.

[0027] The suction and discharge assembly 16 may include a suction member 161 and a discharge member 162. The suction member 161 cooperates with the main body 11 to form a suction channel for conveying the test sample; the discharge member 162 cooperates with the main body 11 to form a discharge channel for conveying the test sample; the suction member 161 is used to suck the test sample from the reagent box B, so that the main body 11 is used to perform impedance counting on the test sample and obtain the test result; when the blood cell counting mechanism 1 completes the impedance counting, the discharge member 162 is used to discharge the completed test sample to the reagent box B. It can be understood that the main body 11 of the blood cell counting mechanism 1 is used as a detection area, the main body 11 is connected to the suction member 161 through the suction channel, and the main body 11 is connected to the discharge member 162 through the discharge channel. When the suction member 161 sucks the test sample from the first liquid pool, the test sample sucked by the suction member 161 enters the main body 11 through the suction channel, so that the main body 11 performs impedance counting on the test sample and obtains the test result. After the blood cell counting mechanism 1 completes the impedance counting, the liquid discharge member 162 is used to discharge the test sample that has completed the test in the main body 11 to the rear cavity of the reagent kit B.

[0028] The reagent box B may include multiple liquid pools for storing samples, reagents and / or mixed liquids, respectively. Exemplarily, the reagent box B may include a first liquid pool and a second liquid pool, wherein the first liquid pool is used to store a mixed liquid of reagents and samples, i.e., a test sample; the liquid aspirator 161 and the liquid discharger 162 may perform liquid aspirating and liquid discharging operations in the first liquid pool of the reagent box B, and the liquid aspirator 161 may also perform liquid aspirating operations in the first liquid pool of the reagent box B, and perform liquid discharging operations on the second liquid pool of the reagent box B through the liquid discharger 162, which is not specifically limited here.

[0029] In the embodiment of the present application, the POCT blood cell counter A receives and transmits the reagent kit B through the pipetting mechanism 4, and the reagent kit B is used to store samples and / or reagents; the blood cell counting mechanism 1 of the POCT blood cell counter A is arranged in the housing 5, the main body 11 of the blood cell counting mechanism 1 is fixedly installed in the housing 5, the suction and discharge assembly 16 is connected to the main body 11 through a pipeline, and the first driving member 15 is used to drive the suction and discharge assembly 16 to move relative to the reagent kit B to complete the suction and / or discharge operation. In the above manner, the first driving member 15 only drives the suction and discharge assembly 16 to move relative to the reagent kit B to complete the suction and discharge operation, and the main body 11 fixed in the housing 5 can perform impedance counting on the sample in the reagent kit B through the suction and discharge operation of the suction and discharge assembly 16, and the reagent kit B can also achieve rapid detection without the need for a separate counting mechanism, which is conducive to reducing the manufacturing cost of the reagent kit B.

[0030] In one embodiment, the suction and discharge assembly 16 includes a liquid suction member 161, a liquid discharge member 162 and a base body 10, wherein the liquid suction member 161 and the liquid discharge member 162 are arranged on the base body 10, and the base body 10 is connected to a first driving member 15, and the first driving member 15 is used to drive the base body 10 to move, so that the liquid suction member 161 and the liquid discharge member 162 can move to a preset position relative to the reagent box B, thereby enabling the liquid suction member 161 and the liquid discharge member 162 to perform suction and discharge operations on the liquid in the reagent box B. The main body 11 also includes a liquid suction joint 114 and a liquid discharge joint 115, wherein the liquid suction member 161 is connected to the liquid suction joint 114 through a pipeline, and the liquid discharge member 162 is connected to the liquid discharge joint 115 through a pipeline.

[0031] Specifically, the first driving member 15 is used to drive the seat 10 to move so that the liquid absorbing member 161 moves to the liquid absorbing position of the reagent kit B. The liquid absorbing member 161 is used to absorb the test sample in the reagent kit B, so that the liquid absorbed by the liquid absorbing member 161 can enter a certain cavity of the main body 11 through the pipeline and the liquid absorbing joint 114 for impedance counting. After the impedance counting is completed, the test sample of the main body 11 can be discharged through the pipeline and the liquid discharge joint 115, and the test sample is discharged into the reagent kit B through the liquid discharge member 162. Therefore, the POCT blood cell counter A of this embodiment can absorb the test sample of the reagent kit B through the liquid absorbing member 161 and allow the test sample to enter the main body 11 through the pipeline and perform impedance counting. After the impedance counting is completed, the test sample that has been tested is discharged to the reagent kit B through the liquid discharge member 162, so as to realize the rapid detection of the reagent kit B; the test sample that has been tested is discharged into the reagent kit B, and there is no need to set up an additional sample waste liquid collection device in the housing 5, thereby reducing the structural space of the POCT blood cell counter A and further reducing the manufacturing cost of the POCT blood cell counter A.

[0032] Illustratively, in one embodiment, the liquid-absorbing member 161 is used to absorb the test sample from the first liquid pool, and the liquid-discharging member 162 is used to discharge the test sample that has completed the test into the first liquid pool. In another embodiment, the liquid-absorbing member 161 is used to absorb the test sample from the first liquid pool, and the liquid-discharging member 162 is used to discharge the test sample that has completed the test into other liquid pools of the reagent kit B except the first liquid pool. Illustratively, the reagent kit B may also include a dilution pool for performing a dilution operation, a sample pool for storing samples, a reagent pool for storing reagents, etc., and the liquid-discharging member 162 may be used to discharge the test sample that has completed the test into any of the dilution pools, sample pools, reagent pools, and the first liquid pool, without specific limitation herein.

[0033] In one embodiment, if Figure 2 As shown, the main body 11 is provided with a front cavity 111 and a rear cavity 112, and the blood cell counting mechanism 1 also includes a first electrode 13, a second electrode 14 and a microporous sheet 12. The microporous sheet 12 is provided with micropores, and the front cavity 111 and the rear cavity 112 are connected through the micropores. The first electrode 13 is penetrated in the rear cavity 112, and the second electrode 14 is used to be electrically connected to the detection sample in the front cavity 111. The liquid absorption component 161 is connected to the front cavity 111 through the liquid absorption channel, and the liquid discharge component 162 is connected to the rear cavity 112 through the liquid discharge channel. The liquid absorption component 161 is used to absorb the detection sample in the first liquid pool so that the detection sample enters the front cavity 111, and the detection sample in the front cavity 111 enters the rear cavity 112 through the micropores. The liquid discharge component 162 is used to discharge the detection sample in the rear cavity 112.

[0034] Specifically, the front chamber 111 of the main body 11 can also be called the front pool, and the rear chamber 112 can also be called the rear pool or the waste liquid chamber, etc. The microporous sheet 12 is used to isolate the front chamber 111 and the rear chamber 112. The microporous sheet 12 is provided with micropores, and the front chamber 111 and the rear chamber 112 are connected through the micropores. The liquid suction joint 114 can be arranged on one side of the front chamber 111, and the liquid discharge joint 115 can be arranged on one side of the rear chamber 112, and the liquid suction joint 114 and the liquid discharge joint 115 can be arranged on the same side wall of the main body 11, so as to facilitate the regular distribution of the liquid suction part 161, the liquid discharge part 162 and the pipeline, and improve the layout aesthetics of the blood cell counting mechanism 1. Among them, the microporous sheet 12 can be called a gemstone hole sheet, a gemstone sheet or a detection sheet, and the micropores can be called gemstone holes or detection holes. For example, the detection sample in the front chamber 111 flows through the micropores into the rear chamber 112 to allow the cells of the detection sample to flow through the micropores one by one. The first electrode 13 is disposed in the rear cavity 112, and one free end of the first electrode 13 may be disposed in the rear cavity 112, and the other end may be located outside the rear cavity 112. The first electrode 13 may be called a rear cell electrode, and the second electrode 14 may be called a front cell electrode.

[0035] The liquid suction piece 161 is connected to the liquid suction joint 114 through a pipeline, and the liquid discharge piece 162 is connected to the liquid discharge joint 115 through a pipeline. The liquid suction piece 161 is used to suck the test sample from the reagent kit B, and make the test sample enter the front chamber 111 through the pipeline and the liquid suction joint 114, and the test sample in the front chamber 111 enters the rear chamber 112 through the micropore. In this process, the first electrode 13, the second electrode 14 and the test sample between the first electrode 13 and the second electrode 14 form a detection circuit. Since blood cells are poor conductors, resistance will be formed when blood cells flow through the micropores and the electric pulse signal between the first electrode 13 and the second electrode 14 will change. The blood cell counting mechanism 1 can characterize the blood cell characteristics of the test sample and obtain the test result by collecting the change of the electric pulse signal between the first electrode 13 and the second electrode 14.

[0036] In a possible implementation, the blood cell counting mechanism 1 can move relative to the reagent kit B; specifically, the blood cell counting mechanism 1 moves, and the reagent kit B does not move; or, the blood cell counting mechanism 1 moves, and the reagent kit B moves; or, the blood cell counting mechanism 1 does not move, and the reagent kit B moves. For example, the reagent kit B is placed on the transfer mechanism 3, and the blood cell counting mechanism 1 can move relative to the position of the reagent kit B, so that the liquid absorbing member 161 can be opposite to the front chamber 111 and absorb the test sample in the front chamber 111.

[0037] In the embodiment of the present application, the POCT blood cell counter A absorbs the test sample in the first liquid pool through the liquid absorption part 161, so that the test sample enters the front chamber 111 through the liquid absorption joint 114, and the test sample in the front chamber 111 enters the rear chamber 112 through the micropores and obtains the test result. The liquid discharge part 162 is used to discharge the test sample in the rear chamber 112 to the rear chamber 112 of the reagent kit B, so that the blood cell counting mechanism 1 can perform impedance counting on the test sample of the reagent kit B, and the reagent kit B can realize rapid detection without setting the microporous sheet 12, and the microporous sheet 12 of the blood cell counting mechanism 1 can be reused, which is beneficial to reduce the manufacturing cost of the reagent kit B, reduce the detection cost of the sample, and improve the practicality of the POCT blood cell counter A.

[0038] Optionally, the main body 11 further includes a quick-release piece 113 , and the microporous sheet 12 is detachably connected to the front cavity 111 via the quick-release piece 113 ; or, the main body 11 is detachably installed in the shell 5 as a whole.

[0039] Specifically, in one embodiment, the main body 11 is detachably installed in the shell 5 as a whole. For example, the main body 11 can be detachably installed in the shell 5 by means of assembly parts such as snaps, screws and bolts, so that the user can replace or maintain the microporous sheet 12 in the main body 11 by disassembling the main body 11. In another embodiment, a mounting hole is provided on the side wall of the front cavity 111, and the quick-release part 113 is installed on the main body 11 through the mounting hole, and the microporous sheet 12 located in the front cavity 111 is limited. When the microporous sheet 12 needs to be replaced, the quick-release part 113 is removed from the mounting hole to separate the quick-release part 113 from the main body 11, so that the user can pull the microporous sheet 12 out of the front cavity 111 through the mounting hole to replace the microporous sheet 12; or, the quick-release part 113 is connected to the microporous sheet 12, and when the quick-release part 113 is removed from the mounting hole, the microporous sheet 12 can be pulled out of the front cavity 111 from the mounting hole along with the quick-release part 113, The user can replace the microporous sheet 12; alternatively, the quick-release piece 113 is arranged on the side facing the opening of the front cavity 111 to confine the second electrode 14 and the microporous sheet 12 in the front cavity 111. When the microporous sheet 12 needs to be replaced, the user can separate the quick-release piece 113 from the main body 11 so that the second electrode 14 and the microporous sheet 12 can fall off the front cavity 111. The user can replace the microporous sheet 12 and, after the replacement, put the replaced microporous sheet 12 and the second electrode 14 back into the front cavity 111 so that the quick-release piece 113 is reconnected with the main body 11 to ensure that the detection function of the blood cell counting mechanism 1 works normally.

[0040] Since the blood cell counting mechanism 1 can be used for multiple impedance counting, the micropores of the microporous sheet 12 may be blocked by liquid, reagent crystallization, etc. after multiple counting, resulting in a high maintenance frequency of the blood cell counting mechanism 1. Therefore, the blood cell counting mechanism 1 of this embodiment can detachably connect the microporous sheet 12 with the front cavity 111 through a quick-release part 113, or the main body 11 of the blood cell counting mechanism 1 can be detachably installed in the housing 5 as a whole, so that the user can disassemble and replace the microporous sheet 12 of the blood cell counting mechanism 1, which is conducive to the regular maintenance of the microporous sheet 12 and reduces the adverse effects of accidents such as blockage on the detection function of the blood cell counting mechanism 1.

[0041] Optionally, see Figure 4 , Figure 4 Schematic diagram of the structure of an embodiment of the pressure building mechanism provided by the present application. Figure 4 As shown, the POCT blood cell counter A of this embodiment also includes a pressure building mechanism 2, which is connected to the rear chamber 112. The pressure building mechanism 2 is used to provide negative pressure to the rear chamber 112 so that the test sample in the front chamber 111 enters the rear chamber 112 through the micropores under the action of negative pressure; the pressure building mechanism 2 is also used to provide positive pressure to the rear chamber 112 so that the test sample in the rear chamber 112 is discharged through the discharge member 162 under the action of positive pressure.

[0042] Specifically, the pressure-building mechanism 2 is connected to the rear chamber 112 and is used to build pressure on the rear chamber 112. For example, the pressure-building mechanism 2 can build pressure through pressure-building components such as a syringe and a pressure pump 21, so that the rear chamber 112 is in a positive pressure environment or a negative pressure environment. Among them, positive pressure usually refers to a pressure environment with a pressure higher than atmospheric pressure, and negative pressure usually refers to a pressure environment with a pressure lower than atmospheric pressure. The front chamber 111 is usually in a pressure environment of normal pressure or atmospheric pressure. When the pressure-building mechanism 2 provides negative pressure to the rear chamber 112, a pressure difference is formed between the front chamber 111 and the rear chamber 112, and the test sample in the front chamber 111 will enter the rear chamber 112 through the micropores under the action of the negative pressure of the rear chamber 112, so that the impedance counting is performed when the test sample flows through the micropores and the corresponding test results are obtained. After the detection is completed, the pressure building mechanism 2 provides positive pressure for the rear chamber 112, one end of the drainage member 162 is connected to the rear chamber 112 through a pipeline and a drainage joint, and the other end of the drainage member 162 is exposed to the atmospheric environment, and a pressure difference is formed between the detection sample in the rear chamber 112 and the drainage member 162, so that the detection sample in the rear chamber 112 is discharged to the rear chamber 112 through the drainage member 162 under the pressure difference.

[0043] In the embodiment of the present application, the POCT blood cell counter A adjusts the pressure environment of the rear chamber 112 through the pressure building mechanism 2, so that when the rear chamber 112 is in a positive pressure environment, the test sample in the front chamber 111 enters the rear chamber 112 through the micropores, and when the rear chamber 112 is in a negative pressure environment, the test sample in the rear chamber 112 is discharged through the discharge member 162 under the action of the positive pressure, so that the blood cell counting mechanism 1 can quickly count the test samples of the test kit B, and the test kit B can achieve rapid detection without the need to set the microporous sheet 12, and the microporous sheet 12 of the blood cell counting mechanism 1 can be reused, which is beneficial to reduce the manufacturing cost of the test kit B, reduce the detection cost of the sample, and improve the practicality of the POCT blood cell counter A.

[0044] Furthermore, the blood cell counting mechanism 1 further includes a switch valve 18, and the drain connector 115 is connected to the rear chamber 112 via the switch valve 18. When the blood cell counting mechanism 1 has completed counting and detecting the test sample, the switch valve 18 is opened, and the pressure building mechanism 2 provides positive pressure to the rear chamber 112. Under the action of the positive pressure, the test sample in the rear chamber 112 can be discharged into the reagent box B through the switch valve 18, the drain connector 115, the pipeline, and the drain member 162 in sequence.

[0045] Among them, since the first electrode 13 is arranged on the side wall of the rear cavity 112 and has a certain height difference with the microporous sheet 12, during the counting process of the detection sample entering the rear cavity 112 from the front cavity 111, the detection sample needs to have a certain amount of accumulation in the rear cavity 112 before the first electrode 13 can contact and electrically connect with the accumulated detection sample. At this time, the switch valve 18 is in a closed state to prevent the detection sample from being directly discharged after entering the rear cavity 112 from the front cavity 111 and unable to be stored in the rear cavity 112, thereby resulting in the inability to achieve electrical connection with the first electrode 13, affecting the counting and detection function of the blood cell counting mechanism 1. After the blood cell counting mechanism 1 completes the counting and detection of the detection sample, the switch valve 18 can be opened, and the detection sample located in the rear cavity 112 can be discharged through the drainage joint 115. And since the drainage joint 115 is arranged at the bottom of the rear cavity 112, it can ensure that the detection sample in the rear cavity 112 is completely discharged.

[0046] It is understandable that, since the channel where the pipette connector 114 is located does not have a valve or other structure, when the pressure-building mechanism 2 provides positive pressure to the rear chamber 112, part of the test sample in the rear chamber 112 will also be discharged from the pipette connector 114, but due to the obstruction of the micropores of the microporous sheet 12, the amount of the test sample discharged from the pipette connector 114 is not large and can be ignored. Therefore, in this embodiment, no valve structure is added to the channel where the pipette connector 114 is located to avoid increasing the volume and cost of the blood cell counting mechanism 1.

[0047] Optionally, the pressure building mechanism 2 includes a pressure pump 21, a first valve 22, a second valve 23 and a three-way connector 24. The first end of the pressure pump 21 is connected to the first end of the three-way connector 24 through the first valve 22, the second end of the pressure pump 21 is connected to the second end of the three-way connector 24 through the second valve 23, and the third end of the three-way connector 24 is connected to the rear cavity 112 of the blood cell counting mechanism 1.

[0048] At this time, when the pressure building mechanism 2 needs to provide negative pressure for the rear chamber 112, the first valve 22 connected to the negative pressure passage of the pressure pump 21 can be opened, and the second valve 23 connected to the positive pressure passage of the pressure pump 21 can be closed, and then the pressure pump 21 provides negative pressure to the rear chamber 112 through the first valve 22 and the first end of the three-way joint 24; when the pressure building mechanism 2 needs to provide positive pressure to the rear chamber 112, the second valve 23 connected to the positive pressure passage of the pressure pump 21 is opened, and the first valve 22 connected to the negative pressure passage of the pressure pump 21 is closed, and the pressure pump 21 provides positive pressure to the rear chamber 112 through the second valve 23 and the second end of the three-way joint 24.

[0049] In one embodiment, if Figure 3 As shown, the blood cell counting mechanism 1 of this embodiment further includes a first driving member 15, a seat 10, a first shielding member 19 and / or a fourth shielding member 151. The liquid suction member 161 and the liquid discharge member 162 are arranged on the seat 10. The first driving member 15 is used to drive the seat 10 to move in the vertical direction so that the liquid suction member 161 and the liquid discharge member 162 move relative to the reagent kit B. The first shielding member 19151 is arranged outside the main body 11 for electromagnetic shielding. The first shielding member 19 is provided with a through hole. The pipeline connecting the main body 11 and the suction and discharge assembly 16 is routed through the through hole provided by the first shielding member 19 to ensure the electromagnetic shielding performance of the first shielding member 19. And / or, the fourth shielding member 151 is arranged outside the first driving member 15, and the fourth shielding member 151 is used to shield the electromagnetic signal of the first driving member 15.

[0050] Specifically, the first driving member 15 can be, but is not limited to, a driving member such as a driving motor or a cylinder that can drive the seat body 10 to move. A liquid absorbing member 161 and a liquid discharging member 162 are installed on one side of the seat body 10, and the main body 11 can be arranged on the side of the blood cell counting mechanism 1 away from the liquid absorbing member 161 and the liquid discharging member 162, and the main body 11 can be fixedly arranged at a certain position of the blood cell counting mechanism 1, or the main body 11 can move with the seat body 10, which is not specifically limited here. The first driving member 15 is used to drive the seat body 10 to move in a vertical direction. For example, the first driving member 15 can drive the seat body 10 to move up and down in a vertical direction so that the liquid absorbing member 161 and the liquid discharging member 162 can be located at different heights.

[0051] Among them, since the main body 11 of the blood cell counting mechanism 1 obtains the detection result by collecting the electric pulse signal between the first electrode 13 and the second electrode 14, the electric pulse signal is usually weak and easily interfered by other factors such as electromagnetic, which easily leads to large errors in impedance counting. Therefore, the blood cell counting mechanism 1 of this embodiment is provided with a first shielding member 19 arranged outside the main body 11, and / or a fourth shielding member 151 arranged outside the first driving member 15, so as to reduce the interference of the electromagnetic signal received by the main body 11, improve the accuracy of impedance counting, and improve the anti-interference performance of the POCT blood cell counter A.

[0052] Optionally, see Figure 5 , Figure 5 Schematic diagram of the structure of the second embodiment of the POCT blood cell counter provided by the present application. Figure 5 As shown, the housing 5 may include a bottom plate and a side plate 51. The bottom plate is used as a bearing seat for structures such as the transfer component, the blood cell counting mechanism 1, and the first driving member 15, and the side plate 51 is arranged around the side of the bottom plate. In this embodiment, when the first shielding member 19 shields the main body 11, the first shielding member 19 forms an accommodating space, and the main body 11 is located in the accommodating space of the first shielding member 19, so that the first shielding member 19 can be used to block electromagnetic signals outside the accommodating space. The side plate 51 is used as a component of the first shielding member 19. For example, the first shielding member 19 may include multiple shielding surfaces, and the side plate 51 is used as one of the shielding surfaces of the first shielding member 19 and is arranged outside the main body 11 for electromagnetic shielding.

[0053] The side panel 51 also includes an opening and closing door 52, which is used to cover or reveal the accommodation space formed by the first shielding member 19 in different states, so as to disassemble the main body 11 as a whole and / or the quick-release member 113. Specifically, the opening and closing door 52 can be rotatably connected to the side panel 51 through a rotating shaft, or the opening and closing door 52 can be detachably connected to the side panel 51 through assembly parts such as snap fasteners, screws and bolts, so that the user can cover or reveal the accommodation space formed by the first shielding member 19 by switching the state of the opening and closing door 52. When the user rotates or disassembles the opening and closing door 52 to reveal the accommodation space, the user can disassemble the main body 11 as a whole in the first shielding member 19 through the exposed position of the opening and closing door 52, or disassemble the quick-release member 113 in the main body 11 through the exposed position of the opening and closing door 52, thereby improving the user's disassembly efficiency, facilitating the user to disassemble and replace the microporous sheet 12, and further improving the user's use experience.

[0054] In one embodiment, the POCT blood cell counter A further comprises a pipetting mechanism 4, which is disposed in the housing 5 and is used to mix reagents and samples in the first liquid pool of the reagent kit B so that the first liquid pool is loaded with the test sample.

[0055] Specifically, reagents and / or samples are stored in the reagent box B. Exemplarily, the reagent box B may include a first liquid pool, a sample pool, and a reagent pool, the sample pool is used to store samples, the reagent pool is used to store reagents, and the pipetting mechanism 4 may be used to perform pipetting operations between the first liquid pool, the sample pool, and the reagent pool to mix the reagents and samples and allow the first liquid pool to store the test sample; or, the reagent box B may include a first liquid pool and a sample pool, the POCT blood cell counter A also includes a reagent mechanism storing reagents, and the pipetting mechanism 4 may be used to perform pipetting operations on the reagents of the reagent mechanism and the samples of the sample pool to mix the reagents and samples and allow the first liquid pool to store the test sample.

[0056] In an embodiment of the present application, the POCT blood cell counter A mixes the reagent and sample in the first liquid pool of the reagent kit B through the pipetting mechanism 4, so that the first liquid pool is loaded with the test sample, thereby reducing the user's manual participation, improving the automated detection level of the POCT blood cell counter A, and thereby improving the detection efficiency of the POCT blood cell counter A.

[0057] Alternatively, if Figure 1 As shown, the transfer mechanism 3 includes a moving guide rail 31, a tray 32 and a second driving member 33. The tray 32 is slidably connected to the moving guide rail 31. The second driving member 33 is connected to the tray 32. The second driving member 33 is used to drive the tray 32 to slide along the moving guide rail 31. The reagent box B includes a plurality of liquid pools arranged linearly. The tray 32 is used to carry the reagent box B. The second driving member 33 is used to drive the reagent box B to move along the extension direction of the moving guide rail 31. The pipetting mechanism 4 is used to perform a pipetting operation on the plurality of liquid pools to mix the reagents and samples and obtain the test samples.

[0058] Specifically, the linear arrangement direction of the reagent box B and the moving direction of the tray 32 are parallel, and the transfer mechanism 3 drives the tray 32 to move in the horizontal direction through the second driving member 33, so as to transfer the reagent box B to the designated pipetting position, detection position, etc. through the tray 32. The reagent box B includes a plurality of liquid pools arranged linearly. When the pipetting mechanism 4 performs a pipetting operation on the plurality of liquid pools, the second driving member 33 is used to drive the tray 32 to move in the horizontal direction and make the plurality of liquid pools respectively located under the pipetting mechanism 4, so that the pipetting mechanism 4 can directly absorb the liquid of the corresponding liquid pool or discharge the liquid to the corresponding liquid pool, so that the pipetting operation of the POCT blood cell counter A of this embodiment is simple, and the motor drives the tray 32 to move in only one direction, and the driving space is small, which is conducive to the miniaturization application of the POCT blood cell counter A.

[0059] Alternatively, if Figure 1 As shown, the transfer mechanism 3 further includes a second shielding member 34 . The second shielding member 34 is disposed on the second driving member 33 . The second shielding member 34 is used to shield the second driving member 33 from electromagnetic signals.

[0060] Specifically, the second shielding member 34 is covered on the second driving member 33, and the second shielding member 34 is used to shield the electromagnetic signal of the second driving member 33, so that the electromagnetic signal generated by the second driving member 33 during the driving process is not easy to interfere with the components other than the second shielding member 34, further reducing the electromagnetic interference during impedance counting and improving the accuracy of blood cell counting.

[0061] In one embodiment, if Figure 1 As shown, the pipetting mechanism 4 includes a third driving member 41, an adapter head 42 and a third shielding member 43. The third driving member 41 is connected to the adapter head 42. The third driving member 41 is used to drive the adapter head 42 to move in a vertical direction. The adapter head 42 is used to plug in a pipette head and perform pipetting operations through the pipette head. The third shielding member 43 is covered on the third driving member 41. The third shielding member 43 is used to shield the third driving member 41 from electromagnetic signals.

[0062] Specifically, the third driving member 41 of the liquid transfer mechanism 4 is used to drive the adapter head 42 to move downward in the vertical direction, so that the pipette head plugged into the adapter head 42 can be located below the liquid surface of the liquid pool of the reagent box B, so that the pipette head can be used to absorb the sample and / or reagent in the liquid pool. The third driving member 41 is used to drive the adapter head 42 to move upward in the vertical direction, and to enable the pipette head to be located above the moving path of the reagent box B. The second driving member 33 is used to drive the tray 32 to move in the horizontal direction so that the multiple liquid pools are respectively located below the pipette head. The pipette head can discharge the absorbed liquid to the corresponding liquid pool to achieve mixing of the reagent and the sample and allow the first liquid pool to store the test sample. The third shielding member 43 is covered on the third driving member 41. The third shielding member 43 is used to shield the electromagnetic signal of the third driving member 41, so that the electromagnetic signal generated by the third driving member 41 during the liquid transfer process is not easy to interfere with the components other than the third shielding member 43, further reducing the electromagnetic interference received during impedance counting, and improving the accuracy of blood cell counting.

[0063] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A POCT blood cell counter, characterized in that: include: case; A transfer mechanism, disposed in the housing, for receiving and transferring a reagent kit, wherein the reagent kit is used to store samples and / or reagents; a first driving member; A blood cell counting mechanism is disposed in the housing, and the blood cell counting mechanism comprises: A main body, fixedly installed in the shell; A suction and discharge assembly, wherein the suction and discharge assembly is connected to the main body through a pipeline; The first driving member is used to drive the aspiration and discharge assembly to move relative to the reagent box to complete aspiration and / or discharge operations.

2. The POCT blood cell counter according to claim 1, characterized in that: The suction and discharge assembly comprises a liquid suction member, a liquid discharge member and a seat body, wherein the liquid suction member and the liquid discharge member are arranged on the seat body, and the seat body is connected to the first driving member; The main body also includes a liquid suction joint and a liquid discharge joint. The liquid suction piece is connected to the liquid suction joint through a pipeline, and the liquid discharge piece is connected to the liquid discharge joint through a pipeline.

3. The POCT blood cell counter according to claim 1, characterized in that: The main body is provided with a front cavity and a rear cavity, and the blood cell counting mechanism further comprises a microporous sheet, the microporous sheet is provided with micropores, and the front cavity and the rear cavity are connected through the micropores; The main body further comprises a quick-release part, and the microporous sheet is detachably connected to the front cavity via the quick-release part; or, the main body as a whole is detachably installed in the shell.

4. The POCT blood cell counter according to claim 1, characterized in that: The blood cell counting mechanism also includes a first shielding component, which is arranged outside the main body to perform electromagnetic shielding. The first shielding component is provided with a through hole, and the pipeline passes through the through hole to connect the suction and discharge assembly and the main body.

5. The POCT blood cell counter according to claim 4, characterized in that: The shell includes a side plate, which is a component of the first shielding member. The side plate also includes an opening and closing door, which is used to shield or reveal the accommodating space formed by the first shielding member in different states so as to disassemble the entire body and / or the quick-release parts.

6. The POCT blood cell counter according to claim 1, characterized in that: The main body is provided with a front cavity and a rear cavity, and the blood cell counting mechanism further comprises a microporous sheet, the microporous sheet is provided with micropores, and the front cavity and the rear cavity are connected through the micropores; The suction and discharge assembly includes a liquid suction component and a liquid discharge component; the liquid suction component is connected to the front cavity, and the liquid discharge component is connected to the rear cavity; The POCT blood cell counter also includes a pressure building mechanism, which is connected to the rear chamber. The pressure building mechanism is used to provide negative pressure to the rear chamber so that the test sample of the test kit enters the front chamber through the liquid absorption piece under the action of negative pressure, and enters the rear chamber through the micropores; the pressure building mechanism is also used to provide positive pressure to the rear chamber so that the test sample in the rear chamber is discharged through the liquid discharge piece under the action of positive pressure.

7. The POCT blood cell counter according to claim 1, characterized in that: The POCT blood cell counter also includes a pipetting mechanism, which is disposed in the housing and is used to mix the reagent and the sample in the first liquid pool of the reagent kit so that the first liquid pool is loaded with the test sample.

8. The POCT blood cell counter according to claim 7, characterized in that: The transfer mechanism comprises a movable guide rail, a tray and a second driving member, wherein the tray is slidably connected to the movable guide rail, the second driving member is connected to the tray, and the second driving member is used to drive the tray to slide along the movable guide rail; The reagent box includes a plurality of liquid pools arranged linearly, the tray is used to carry the reagent box, the second driving member is used to drive the reagent box to move along the extension direction of the movable guide rail, and the pipetting mechanism is used to perform pipetting operations on the plurality of liquid pools to mix the reagents and the samples and obtain the test samples.

9. The POCT blood cell counter according to claim 8, characterized in that: The transfer mechanism further includes a second shielding member, which is disposed on the second driving member and is used to shield the second driving member from electromagnetic signals.

10. The POCT blood cell counter according to claim 7, characterized in that: The pipetting mechanism includes a third driving member, an adapter head and a third shielding member. The third driving member is connected to the adapter head. The third driving member is used to drive the adapter head to move in a vertical direction. The adapter head is used to plug in a pipette head and perform pipetting operations through the pipette head. The third shielding member is covered on the third driving member. The third shielding member is used to shield the third driving member from electromagnetic signals.

Images