Stem cell collecting and extracting equipment

Abstract

The utility model relates to the technical field of stem cell extraction. The stem cell collection and extraction equipment comprises a filter cylinder, a culture cylinder connected to the bottom of the filter cylinder and a centrifugal device installed on one side of the culture cylinder, and a filter screen is arranged in the filter cylinder in a sleeved mode. A material guide nozzle is arranged on one side of the cultivation cylinder along the top opening, a material guide pipe is arranged in the material guide nozzle in a penetrating mode, and the outer end of the material guide pipe is connected with the connecting pipe through a micro pump. A filtering inner cylinder is sleeved with a centrifugal outer cylinder of the centrifugal device, and a main shaft of a driving motor at the bottom of the centrifugal outer cylinder upwards penetrates through the centrifugal outer cylinder and then is connected with the filtering inner cylinder. Large-particle tissue fragments can be filtered out by the filter screen, stem cell tissue fluid leaks downwards into the culture cylinder, culture and decomposition of stem cells can be completed in the culture cylinder, the stem cell tissue fluid can be guided into the filter inner cylinder through the micro pump, the filter inner cylinder is driven by the driving motor to rotate at a high speed, and target stem cells can be obtained under the action of centrifugal force. Transfer among the steps is rapid and convenient, and the extraction period can be greatly shortened.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell extraction, in particular to a stem cell collection and extraction device. Background Art

[0002] Stem cells have the functions of self-renewal and differentiation. The application of stem cells in the field of regenerative medicine is quite promising, especially for tissue replacement, which is an effective tool for regeneration or repair. Usually, stem cell tissues are collected and cultured, and then the required stem cells are extracted from the culture medium. Usually, the extraction process requires first crushing the cell tissue in the culture medium, then filtering out the crushed cell tissue in the culture medium, and finally using a centrifugal device to centrifuge the culture medium to obtain the target stem cells. Existing stem cell extraction equipment is divided into multiple steps. The main steps are crushing and filtration, cell culture, and centrifugation. The equipment that completes each step is an independent structure. The transfer between each step is time-consuming, resulting in a relatively long extraction cycle. In response to the above problems, we invented a stem cell collection and extraction device. Utility Model Content

[0003] The utility model provides a stem cell collection and extraction device which solves the above technical problems.

[0004] The technical solution of the utility model is implemented as follows: the stem cell collection and extraction equipment includes a filter cartridge and a culture cartridge connected to the bottom of the filter cartridge, and a centrifugal device installed on one side of the culture cartridge; the filter cartridge is connected from top to bottom, a filter screen is sleeved inside the filter cartridge, a cover is installed along the top of the filter cartridge, and a feed nozzle is provided on the cover; a heating component is installed in the culture cartridge, a feeding nozzle is provided along one side of the top of the culture cartridge, a material guide nozzle is provided along the other side, a slag discharge nozzle is provided along the bottom of one side of the culture cartridge, and a first Switch valve; a material guide pipe is inserted into the material guide nozzle, the inner end of the material guide pipe extends into the cultivation cylinder, the outer end of the material guide pipe extends out of the material guide nozzle and is connected to a micro pump, and a connecting pipe is installed on the output end of the micro pump; the centrifugal device includes a centrifugal outer cylinder and a filtering inner cylinder sleeved in the centrifugal outer cylinder, a driving motor is installed at the bottom of the centrifugal outer cylinder, the main shaft of the driving motor passes upward through the centrifugal outer cylinder and is connected to the filtering inner cylinder; a second switch valve is installed at the top of the filtering inner cylinder after passing through the centrifugal outer cylinder, and the end of the connecting pipe is movably connected to the second switch valve.

[0005] Furthermore, the filter inner cylinder is provided with a plurality of installation grooves along the side wall, and filter plates are installed in the installation grooves; the filter inner cylinder is provided with a limiting ring along the bottom, and a pressure bearing is sleeved in the limiting ring, and the pressure bearing abuts against the inner bottom surface of the centrifugal outer cylinder.

[0006] Furthermore, the heating assembly includes a control box installed at the bottom of the cultivation cylinder, and a fixed plate installed inside the cultivation cylinder, a plurality of heating wires are installed on the fixed plate, and the control box is electrically connected to the heating wires.

[0007] Furthermore, the material guide tube is an elastic corrugated hose structure, a filter head is installed at the end of the material guide tube, and the filter head is located in the middle of the cultivation cylinder.

[0008] By adopting the above technical scheme, the utility model has the following beneficial effects: after the collected stem cell tissue is crushed, the filter net can filter out large particles of tissue fragments, and the stem cell tissue fluid can leak into the culture tube, and the stem cell culture and decomposition can be completed in the culture tube, and then the stem cell tissue fluid can be introduced into the filter inner tube through the micro pump, and the driving motor drives the filter inner tube to rotate at a high speed, and the stem cell tissue fluid is separated under the action of centrifugal force to obtain the target stem cells. Each step is closely connected, and the transfer between each step is relatively fast and convenient, which can greatly shorten the extraction cycle. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0010] Figure 1 It is a schematic diagram of the structure of the utility model;

[0011] Figure 2 This is a schematic diagram of the split structure of the utility model;

[0012] Among them: 1. filter cylinder; 2. cultivation cylinder; 3. filter screen; 4. cover; 5. feed nozzle; 6. feeding nozzle; 7. guide nozzle; 8. slag discharge nozzle; 9. first switch valve; 10. guide pipe; 11. micro pump; 12. connecting pipe; 13. centrifugal outer cylinder; 14. filter inner cylinder; 15. drive motor; 16. second switch valve; 17. installation groove; 18. filter plate; 19. limit ring; 20. pressure bearing; 21. control box; 22. fixing plate; 23. heating wire; 24. filter head. DETAILED DESCRIPTION

[0013] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0014] like Figure 1-2 As shown, the stem cell collection and extraction device includes a filter cartridge 1 and a culture cartridge 2 connected to the bottom of the filter cartridge 1, and a centrifugal device installed on one side of the culture cartridge 2. The filter cartridge 1 is through-through from top to bottom, and a filter screen 3 is sleeved inside the filter cartridge 1. A cover 4 is installed along the top of the filter cartridge 1, and a feed nozzle 5 is provided on the cover 4. A heating component is installed in the culture cartridge 2, and a feed nozzle 6 is provided along one side of the top of the culture cartridge 2, and a feed guide nozzle 7 is provided along the other side. A slag discharge nozzle 8 is provided along the bottom of one side of the culture cartridge 2, and a first switch valve 9 is installed on both the feed nozzle 6 and the slag discharge nozzle 8. A feed guide pipe 10 is inserted into the feed guide nozzle 7, and the inner end of the feed guide pipe 10 extends into the culture cartridge 2. The outer end of the feed guide pipe 10 extends out of the feed guide nozzle 7 and is connected to a micro pump 11, and a connecting pipe 12 is installed on the output end of the micro pump 11. The centrifugal device includes a centrifugal outer cylinder 13 and a filter inner cylinder 14 sleeved in the centrifugal outer cylinder 13. A driving motor 15 is installed at the bottom of the centrifugal outer cylinder 13. The main shaft of the driving motor 15 passes through the centrifugal outer cylinder 13 upward and is connected to the filter inner cylinder 14. A second switch valve 16 is installed at the top of the filter inner cylinder 14 after passing through the centrifugal outer cylinder 13. The end of the connecting pipe 12 is movably connected to the second switch valve 16.

[0015] The filter inner cylinder 14 of this embodiment is provided with a plurality of mounting grooves 17 along the side wall, and a filter plate 18 is installed in the mounting grooves 17. A limit ring 19 is provided along the bottom of the filter inner cylinder 14, and a pressure bearing 20 is sleeved in the limit ring 19, and the pressure bearing 20 abuts against the inner bottom surface of the centrifugal outer cylinder 13. The driving motor 15 can drive the filter inner cylinder 14 to rotate at a high speed, so that the stem cell tissue fluid in the filter inner cylinder 14 performs centrifugal motion, and tiny water molecules can be thrown into the centrifugal outer cylinder 13 to obtain target stem cells. The filter inner cylinder 14 has high requirements for structural strength. The filter inner cylinder 14 is provided with a plurality of mounting grooves 17 distributed alternately, which has little effect on the structural strength and can accelerate the efficiency of centrifugal filtration.

[0016] The heating assembly of this embodiment includes a control box 21 installed at the bottom of the culture tube 2, and a fixed plate 22 installed inside the culture tube 2. A plurality of heating wires 23 are installed on the fixed plate 22, and the control box 21 is electrically connected to the heating wires 23. The heating wires 23 can be used to heat and keep the stem cell tissue fluid and the culture fluid in the culture tube 2 warm, and no external objects are needed to stabilize the environmental temperature during stem cell cultivation.

[0017] The guide tube 10 of this embodiment is an elastic corrugated hose structure, and a filter head 24 is installed at the end of the guide tube 10, and the filter head 24 is located in the middle of the culture tube 2. The filter head 24 can be used to extract the supernatant in the culture tube 2, and the stem cell tissue fluid can be drawn into the centrifugal device. Since the guide tube 10 has elastic elasticity, the filter head 24 will not sink to the bottom of the culture tube 2 under the action of buoyancy, and the supernatant in the culture tube 2 can be smoothly extracted.

[0018] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A stem cell collection and extraction device, characterized in that: The invention comprises a filter cartridge (1) and a cultivation cartridge (2) connected to the bottom of the filter cartridge (1), and a centrifugal device installed on one side of the cultivation cartridge (2); the filter cartridge (1) is connected from top to bottom, a filter screen (3) is sleeved inside the filter cartridge (1), a cover (4) is installed along the top of the filter cartridge (1), and a feed nozzle (5) is provided on the cover (4); a heating component is installed inside the cultivation cartridge (2), a feeding nozzle (6) is provided along one side of the top of the cultivation cartridge (2), and a material guide nozzle (7) is provided along the other side; a slag discharge nozzle (8) is provided along the bottom of one side of the cultivation cartridge (2), and a first switch valve (9) is installed on both the feeding nozzle (6) and the slag discharge nozzle (8); a material guide pipe (10) is passed through the material guide nozzle (7), and the The inner end of the material guide pipe (10) extends into the culture cylinder (2), and the outer end of the material guide pipe (10) extends out of the material guide nozzle (7) and is connected to a micro pump (11), and a connecting pipe (12) is installed on the output end of the micro pump (11); the centrifugal device comprises a centrifugal outer cylinder (13) and a filtering inner cylinder (14) sleeved in the centrifugal outer cylinder (13), a driving motor (15) is installed at the bottom of the centrifugal outer cylinder (13), and the main shaft of the driving motor (15) passes through the centrifugal outer cylinder (13) upward and is connected to the filtering inner cylinder (14); the top of the filtering inner cylinder (14) passes through the centrifugal outer cylinder (13) and is installed with a second switch valve (16), and the end of the connecting pipe (12) is movably connected to the second switch valve (16).

2. The stem cell collection and extraction device according to claim 1, characterized in that: The filter inner cylinder (14) is provided with a plurality of mounting grooves (17) along the side wall, and a filter plate (18) is installed in the mounting groove (17); the filter inner cylinder (14) is provided with a limiting ring (19) along the bottom, and a pressure bearing (20) is sleeved in the limiting ring (19), and the pressure bearing (20) abuts against the inner bottom surface of the centrifugal outer cylinder (13).

3. The stem cell collection and extraction device according to claim 1, characterized in that: The heating assembly comprises a control box (21) installed at the bottom of the cultivation cylinder (2), and a fixing plate (22) installed inside the cultivation cylinder (2), a plurality of heating wires (23) are installed on the fixing plate (22), and the control box (21) is electrically connected to the heating wires (23).

4. The stem cell collection and extraction device according to claim 1, characterized in that: The material guide tube (10) is an elastic corrugated hose structure. A filter head (24) is installed at the end of the material guide tube (10), and the filter head (24) is located in the middle of the cultivation cylinder (2).

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