Isothermal block and temperature calibration system suitable for calibrating temperature detection device

Abstract

The utility model provides an isothermal block and a temperature calibration system suitable for calibrating a temperature detection device. The isothermal block is suitable for calibrating the temperature detection device, the temperature detection device comprises at least one temperature sensor, and the isothermal block comprises a working part and a temperature sensor, at least one isothermal hole extending downwards is formed in the bottom of the main shell, and the isothermal hole is configured to allow the temperature sensor to extend in and be attached to the temperature sensor; each pressing lock catch comprises a second part which is installed on the main shell and installed on the cover plate, a rotating part responding to the second part rotates to be buckled with the first part, the cover plate is fixed to the top of the main shell to form a containing space, the working part is soaked in the constant-temperature groove, and the pressing lock catches are arranged in the containing space. The temperature sensor detects the temperature of the thermostatic bath; the transition cavity is connected with the working part, and the transition cavity extends out of the thermostatic bath.

Description

Technical Field

[0001] At least one embodiment of the utility model relates to the technical field of real-time fluorescence quantitative PCR instrument calibration, and in particular to an isothermal block and a temperature calibration system suitable for calibrating a temperature detection device. Background Art

[0002] A real-time PCR instrument can monitor and quantitatively amplify DNA or RNA in real time during the polymerase chain reaction (PCR) process, so that users can conduct research and diagnosis based on the detection and amplification results. Therefore, the accuracy of the real-time PCR instrument is crucial to the results of users' research and diagnosis.

[0003] The calibration system for calibrating the real-time fluorescence quantitative PCR instrument can use a temperature detection device to calibrate the real-time fluorescence quantitative PCR instrument. The accuracy of the temperature detection device affects the accuracy of calibrating the real-time fluorescence quantitative PCR instrument. Therefore, it is necessary to improve the accuracy of the calibration temperature detection device.

[0004] In the process of implementing the embodiments of the utility model, the inventors found that the temperature calibration system in the related art has the defect of low calibration accuracy. Utility Model Content

[0005] The embodiments of the utility model provide an isothermal block and a temperature calibration system suitable for calibrating a temperature detection device, which are used to at least partially solve the above-mentioned problems, improve the calibration accuracy of the calibrated temperature detection device, reduce the calibration time, and improve work efficiency.

[0006] In order to achieve the above-mentioned purpose, the utility model provides an isothermal block suitable for calibrating a temperature detection device, the temperature detection device includes at least one temperature sensor, the isothermal block includes: a working part, including: a cover plate; a main shell, the bottom of the main shell is formed with at least one isothermal hole extending downward, the isothermal hole is configured to allow the temperature sensor to extend into and fit with the temperature sensor; and a plurality of clamping locks, each of the clamping locks includes a second part mounted on the cover plate installed on the main shell, in response to the rotating member of the second part rotating to fit with the first part, the cover plate is fixed on the top of the main shell to form a accommodating space, wherein, in response to the working part being immersed in the constant temperature bath, the temperature sensor detects the temperature of the constant temperature bath; a transition chamber connected to the working part, the transition chamber is configured to extend out of the constant temperature bath.

[0007] Optionally, the isothermal block also includes: a fixing portion, connected to the transition chamber and configured to be connected to the constant temperature bath to fix the isothermal block, wherein a standard thermometer jack is provided on the fixing portion, and the standard thermometer jack is configured to allow a standard thermometer to be inserted, and in response to the working portion being immersed in the constant temperature bath, the standard thermometer detects the temperature of the constant temperature bath.

[0008] Optionally, an alignment protrusion extending upward is formed on one side edge of the top of the main shell, and an alignment groove matching the shape of the alignment protrusion is formed on one side edge of the cover plate corresponding to the main shell.

[0009] Optionally, the isothermal hole is a blind hole.

[0010] Optionally, an annular sealing groove is formed on the top of the main shell, and a sealing protrusion that cooperates with the sealing groove is formed at a position corresponding to the cover plate and the main shell. In response to the cooperation between the sealing groove and the sealing protrusion, the cover plate and the main shell form a accommodating space.

[0011] Optionally, an elastic member is provided between the sealing protrusion and the sealing groove.

[0012] Optionally, the fixing portion further includes: a through hole, based on the transition cavity being in communication with the accommodating space, a wire connected to the temperature sensor extends from the accommodating space out of the through hole to be connected to an external host computer.

[0013] Optionally, the isothermal wells are distributed in a 12×8 array.

[0014] Optionally, two symmetrically arranged handles are provided on the fixing portion.

[0015] The utility model also provides a temperature calibration system suitable for calibrating a temperature detection device, wherein the temperature detection device is configured to detect the temperature of a heating hole in a real-time fluorescence quantitative PCR instrument, and the temperature calibration system comprises: a constant temperature bath; an isothermal block as described above, wherein a working portion of the isothermal block is configured to be immersed in the constant temperature bath; a standard thermometer, which is arranged in a standard thermometer jack of the isothermal block and inserted into the constant temperature bath; and a host computer, which is connected to the standard thermometer and a temperature detection device arranged in the isothermal hole of the isothermal block, and is configured to determine a calibration value based on a first temperature value measured by the temperature detection device and a second temperature value measured by the standard thermometer.

[0016] According to an embodiment of the utility model, at least one isothermal hole extending downward is formed at the bottom of the main shell, and each isothermal hole is distributed at intervals. After the temperature sensor is installed in the isothermal hole, the temperature sensor detects the temperature of the thermostatic bath based on the isothermal hole, which can shorten the time required for thermal stabilization and improve the detection efficiency of the temperature sensor. By setting a transition chamber, the working part can be immersed in a deeper position of the thermostatic bath, thereby improving the stability of the temperature sensor detection and the calibration accuracy of the calibration temperature detection device. By installing the temperature sensor in the isothermal hole when the main shell and the cover plate are separated, and fixing the cover plate on the top of the main shell after installation, the installation stability of the temperature sensor can be improved, thereby improving the accuracy of the temperature sensor detecting the temperature, so as to achieve the purpose of improving the calibration accuracy of the calibration temperature detection device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The above and other purposes, features and advantages of the present invention will become more apparent through the following description of the embodiments of the present invention with reference to the accompanying drawings, in which:

[0018] Figure 1 It is a stereoscopic diagram of an isothermal block according to an illustrative embodiment of the utility model.

[0019] Figure 2 is a side view of an isothermal block according to an illustrative embodiment of the present invention.

[0020] Figure 3 It is a cross-sectional view of a main housing according to an illustrative embodiment of the utility model.

[0021] Figure 4 It is a side view of a main housing according to an illustrative embodiment of the utility model.

[0022] Figure 5 It is a side view of the main housing at another angle according to an illustrative embodiment of the utility model.

[0023] Figure 6 It is a side view of a cover plate according to an illustrative embodiment of the utility model.

[0024] Figure 7 It is a side view of a cover plate at another angle according to an illustrative embodiment of the utility model.

[0025] In the above drawings, the meanings of the reference numerals are as follows:

[0026] 1. Work Department;

[0027] 11. Cover plate;

[0028] 111. Alignment groove;

[0029] 112, sealing protrusion;

[0030] 12. Main shell;

[0031] 121. Isothermal hole;

[0032] 122. Alignment protrusions;

[0033] 1221, Part III;

[0034] 1222, Part IV;

[0035] 123, annular sealing groove;

[0036] 13. Press the lock button tightly;

[0037] 131, Part I;

[0038] 132. Part II;

[0039] 2. Transition cavity;

[0040] 3. Fixed part;

[0041] 31. Standard thermometer jack;

[0042] 32. Through hole;

[0043] 33. Handle;

[0044] 4. Temperature sensor. DETAILED DESCRIPTION

[0045] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in combination with specific embodiments and with reference to the accompanying drawings. The terms used herein are only for describing specific embodiments and are not intended to limit the utility model.

[0046] The terms "include", "comprising", etc. used herein indicate the presence of features, steps, operations and / or components, but do not exclude the presence or addition of one or more other features, steps, operations or components. All terms used herein, including technical and scientific terms, have the meanings commonly understood by those skilled in the art unless otherwise defined. It should be noted that the terms used herein should be interpreted as having a meaning consistent with the context of this specification and should not be interpreted in an idealized or overly rigid manner.

[0047] In this document, unless otherwise specified, directional terms such as "upper", "lower", "left", "right", "inner", "outer", etc. are used to indicate the orientation or positional relationship based on the drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device, element or component referred to must have a specific orientation, be constructed or operate in a specific orientation. It should be understood that when the absolute position of the described object changes, the relative positional relationship they represent may also change accordingly. Therefore, these directional terms cannot be understood as limiting the present invention.

[0048] When using expressions such as "at least one of A, B, and C, etc.", it should generally be interpreted as the meaning of the expression generally understood by those skilled in the art. For example, "a system having at least one of A, B, and C" should include but not be limited to a system having A alone, B alone, C alone, A and B, A and C, B and C, and / or A, B, C, etc. When using expressions such as "at least one of A, B, or C, etc.", it should generally be interpreted as the meaning of the expression generally understood by those skilled in the art. For example, "a system having at least one of A, B, or C" should include but not be limited to a system having A alone, B alone, C alone, A and B, A and C, B and C, and / or A, B, C, etc.

[0049] Figure 1 It is a stereoscopic diagram of an isothermal block according to an illustrative embodiment of the utility model. Figure 2 is a side view of an isothermal block according to an illustrative embodiment of the present invention. Figure 3 It is a cross-sectional view of a main housing according to an illustrative embodiment of the utility model. Figure 4 It is a side view of a main housing according to an illustrative embodiment of the utility model. Figure 5 It is a side view of the main housing at another angle according to an illustrative embodiment of the utility model.

[0050] like Figures 1 to 5As shown, the utility model provides an isothermal block. The isothermal block is suitable for calibrating a temperature detection device. The isothermal block can be used to form a temperature calibration system to calibrate the temperature detection device. The temperature detection device may include at least one temperature sensor 4. The temperature sensor 4 can be used to detect the temperature of the heating hole in the real-time fluorescence quantitative PCR instrument to calibrate the real-time fluorescence quantitative PCR instrument based on the detected temperature. The isothermal block may include a working part 1 and a transition chamber 2. The working part 1 may include a cover plate 11, a main shell 12 and a plurality of clamping locks 13. At least one isothermal hole 121 extending downward may be formed at the bottom of the main shell 12. The isothermal hole 121 may be a blind hole. The isothermal hole 121 may be formed as a raised structure at the bottom of the main shell 12. The isothermal hole 121 may be integrally formed with the main shell 12. The isothermal hole 121 is configured to allow the temperature sensor 4 to extend therein and fit with the temperature sensor 4. The isothermal holes 121 may be spaced apart. By extending the temperature sensor 4 into the thermostatic bath to detect the temperature based on the isothermal hole 121, the time required for thermal stabilization can be shortened, thereby improving the detection efficiency. The width of the isothermal hole 121 can be smaller than the width of the temperature sensor 4. The isothermal hole 121 can be made of an elastic material so that after the temperature sensor 4 is extended into the isothermal hole 121, the isothermal hole 121 can fit tightly with the temperature sensor 4, thereby further shortening the time required for thermal stabilization and improving the detection efficiency. The main shell 12 and the cover plate 11 can be made of high-density metal to improve the isothermal performance of the working part 1. The transition chamber 2 can be made of stainless steel. The transition chamber 2 can be connected to the working part 1 by an adhesive. The transition chamber 2 and the working part 1 can also be formed in one piece. The transition chamber 2 can isolate the working medium in the thermostatic bath from the outside, thereby preventing the working medium in the thermostatic bath from being immersed in the working part 1.

[0051] Further, each compression lock buckle 13 may include a first portion 131 mounted on the main housing 12 and a second portion 132 mounted on the cover plate 11. In response to the rotation of the rotating member of the second portion 132 rotating to engage with the first portion 131, the cover plate 11 is fixed to the top of the main housing 12 to form a receiving space. The compression lock buckle 13 may be a spring buckle. When the main housing 12 and the cover plate 11 are separated, the temperature sensor 4 may be installed in the isothermal hole 121. After the temperature sensor 4 is installed in the isothermal hole 121, the cover plate 11 may be covered on the top of the main housing 12, and based on the engagement of the first portion 131 and the second portion 132, the cover plate 11 is fixed to the main housing 12. By installing the temperature sensor 4 in the isothermal hole 121 when the main housing 12 and the cover plate 11 are separated, and fixing the cover plate 11 to the top of the main housing 12 after installation, the installation stability of the temperature sensor 4 can be improved, thereby improving the accuracy of the temperature detection by the temperature sensor 4. Since the temperature detection device includes the temperature sensor 4, the calibration accuracy of the temperature detection device can be improved. Since the calibration system uses the temperature detection device to calibrate the real-time fluorescence quantitative PCR instrument, the improvement of the detection accuracy of the temperature detection device can improve the accuracy of the calibration of the real-time fluorescence quantitative PCR instrument.

[0052] Further, in response to the working part 1 being immersed in the constant temperature bath, the temperature sensor 4 can detect the temperature of the constant temperature bath. The transition chamber 2 is connected to the working part 1. The transition chamber 2 is configured to extend out of the constant temperature bath. Since the working part 1 is connected to the transition chamber 2, the working part 1 can be immersed in a deeper position of the constant temperature bath, for example, the immersion depth can be 37 cm. Thus, the stability of the temperature around the temperature sensor 4 is better, the stability of the detection of the temperature sensor 4 can be improved, and thus the calibration accuracy of the calibration temperature detection device can be improved.

[0053] According to the embodiment of the utility model, at least one isothermal hole 121 extending downward is formed at the bottom of the main shell 12, and each isothermal hole 121 is distributed at intervals. After the temperature sensor 4 is installed in the isothermal hole 121, the temperature sensor 4 detects the temperature of the thermostatic bath based on the isothermal hole 121, which can shorten the time required for thermal stabilization and improve the detection efficiency of the temperature sensor 4. By providing the transition chamber 2, the working part 1 can be immersed in a deeper position of the thermostatic bath, thereby improving the stability of the detection of the temperature sensor 4 and the calibration accuracy of the calibration temperature detection device. By installing the temperature sensor 4 in the isothermal hole 121 when the main shell 12 and the cover plate 11 are separated, and fixing the cover plate 11 on the top of the main shell 12 after installation, the installation stability of the temperature sensor 4 can be improved, thereby improving the accuracy of the temperature detection of the temperature sensor 4, so as to achieve the purpose of improving the calibration accuracy of the calibration temperature detection device.

[0054] In some embodiments, the isothermal block may further include a fixing portion 3. The fixing portion 3 may be connected to the transition chamber 2. The fixing portion 3 may be connected to a constant temperature bath to fix the isothermal block. A standard thermometer jack 31 may be provided on the fixing portion 3. The standard thermometer jack 31 is configured to allow a standard thermometer to be inserted. In response to the working portion 1 being immersed in the constant temperature bath, the standard thermometer detects the temperature of the constant temperature bath. By comparing the first temperature value measured by the temperature detection device and the second temperature value measured by the standard thermometer, the accuracy of the temperature detection device can be confirmed, thereby calibrating the temperature detection device. The standard thermometer jack 31 may be made of an elastic material, so that the standard thermometer can be fixed based on elasticity after the detection end of the standard thermometer reaches the target position by pushing the standard thermometer. The target position may be the position where the temperature sensor 4 is located in the constant temperature bath.

[0055] Figure 6 It is a side view of a cover plate according to an illustrative embodiment of the utility model.

[0056] like Figure 5 and Figure 6 As shown, in some embodiments, an alignment protrusion 122 extending upward is formed on one side edge of the top of the main housing 12. An alignment groove 111 matching the shape of the alignment protrusion 122 is formed on one side edge of the cover plate 11 corresponding to the main housing 12. Based on the cooperation between the alignment groove 111 and the alignment protrusion 122, the cover plate 11 and the main housing 12 can be aligned relatively quickly and accurately. The alignment protrusion 122 may include a third portion 1221 and a fourth portion 1222. The length of the third portion 1221 may be equal to the width of the main housing 12. The fourth portion 1222 may be connected to the third portion 1221 and extend from the center of the third portion 1221 to the other side of the main housing 12.

[0057] Figure 7 It is a side view of a cover plate at another angle according to an illustrative embodiment of the utility model.

[0058] like Figure 7 As shown, in some embodiments, an annular sealing groove 123 is formed on the top of the main housing 12. A sealing protrusion 112 that cooperates with the annular sealing groove 123 is formed at the corresponding position of the cover plate 11 and the main housing 12. In response to the cooperation between the annular sealing groove 123 and the sealing protrusion 112, the cover plate 11 and the main housing 12 form a receiving space.

[0059] In some embodiments, an elastic member may be provided between the sealing protrusion 112 and the annular sealing groove 123. The elastic member may be a sealing rubber strip. The elastic member may be placed in the annular sealing groove 123 and pressed by the sealing protrusion 112 of the cover plate 11. At the same time, the pressing lock 13 may further press the elastic member, thereby preventing the working medium in the thermostatic bath from being immersed in the working part 1.

[0060] In some embodiments, the fixing portion 3 may further include a through hole 32. The through hole 32 may be connected to the accommodation space based on the transition cavity 2. After the temperature sensor 4 is installed in the isothermal hole 121, the wire connected to the temperature sensor 4 may extend from the accommodation space through the through hole 32 to connect to an external host computer.

[0061] In some embodiments, the isothermal wells 121 may be distributed in a 12×8 array to be suitable for calibrating a real-time fluorescence quantitative PCR instrument with 96 wells or less.

[0062] In some embodiments, two symmetrically arranged handles 33 may be provided on the fixing portion 3, so that the isothermal block can be moved more conveniently.

[0063] The utility model also provides a temperature calibration system suitable for calibrating a temperature detection device. The temperature detection device is configured to detect the temperature of a heating hole in a real-time fluorescence quantitative PCR instrument. The temperature calibration system may include a constant temperature bath, an isothermal block, a standard thermometer and a host computer. The working part of the isothermal block is configured to be immersed in the constant temperature bath. The standard thermometer can be set in the standard thermometer jack of the isothermal block and inserted into the constant temperature bath. The host computer is connected to the standard thermometer and the temperature detection device set in the isothermal hole of the isothermal block. The host computer can determine the calibration value of the calibration temperature detection device based on the first temperature value measured by the temperature detection device and the second temperature value measured by the standard thermometer. The second temperature value can be a standard value, and the second temperature value and the first temperature value can be error values ​​of the calibration temperature detection device. Based on the error value, the calibration value of the calibration temperature detection device can be determined, so that the detection temperature of the temperature detection device can reach the standard value.

[0064] The above specific embodiments further illustrate the purpose, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above are only specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An isothermal block, characterized in that: Suitable for calibrating a temperature detection device, the temperature detection device comprising at least one temperature sensor, the isothermal block comprising: Work Department, including: Cover plate; A main housing, wherein at least one isothermal hole extending downward is formed at the bottom of the main housing, and the isothermal hole is configured to allow the temperature sensor to extend into and fit with the temperature sensor; and a plurality of pressing locks, each of the pressing locks comprising a second portion mounted on the cover plate, in response to a rotating member of the second portion rotating to engage with a first portion mounted on the main housing, the cover plate being fixed to the top of the main housing to form a receiving space, wherein in response to the working part being immersed in the constant temperature bath, the temperature sensor detects the temperature of the constant temperature bath; and A transition chamber is connected to the working part, and the transition chamber is configured to extend out of the constant temperature bath.

2. The isothermal block according to claim 1, characterized in that: Also includes: A fixing part is connected to the transition chamber and is configured to be connected to the constant temperature bath to fix the isothermal block, wherein a standard thermometer jack is provided on the fixing part, and the standard thermometer jack is configured to allow a standard thermometer to be inserted, and in response to the working part being immersed in the constant temperature bath, the standard thermometer detects the temperature of the constant temperature bath.

3. The isothermal block according to claim 1, characterized in that: An alignment protrusion extending upward is formed on one side edge of the top of the main shell, and an alignment groove matching the shape of the alignment protrusion is formed on one side edge of the cover plate corresponding to the main shell.

4. The isothermal block according to claim 1, characterized in that: The isothermal hole is a blind hole.

5. The isothermal block according to claim 1, characterized in that: An annular sealing groove is formed on the top of the main shell, and a sealing protrusion that cooperates with the sealing groove is formed at a position corresponding to the cover plate and the main shell. In response to the cooperation between the sealing groove and the sealing protrusion, the cover plate and the main shell form a accommodating space.

6. The isothermal block according to claim 5, characterized in that: An elastic member is arranged between the sealing protrusion and the sealing groove.

7. The isothermal block according to claim 2, characterized in that: The fixing portion further comprises: The through hole is connected with the accommodating space based on the transition cavity, and the wire connected with the temperature sensor extends from the accommodating space out of the through hole to be connected with an external host computer.

8. The isothermal block according to claim 1, characterized in that: The isothermal wells are distributed in a 12×8 array.

9. The isothermal block according to claim 2, characterized in that: The fixing portion is provided with two symmetrically arranged handles.

10. A temperature calibration system suitable for calibrating a temperature detection device, characterized in that: The temperature detection device is configured to detect the temperature of a heating hole in a real-time fluorescence quantitative PCR instrument, and the temperature calibration system comprises: Constant temperature bath; The isothermal block as claimed in any one of claims 2 to 9 above, wherein the working portion of the isothermal block is configured to be immersed in the constant temperature bath; A standard thermometer is disposed in a standard thermometer jack of the isothermal block and inserted into the constant temperature bath; and The host computer is connected to the standard thermometer and the temperature detection device arranged in the isothermal hole of the isothermal block, and is configured to determine the calibration value based on the first temperature value measured by the temperature detection device and the second temperature value measured by the standard thermometer.

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