T cell sorting magnetic bead assembly

Abstract

The utility model discloses a T cell sorting magnetic bead assembly which comprises a box body and a T cell sorting magnetic bead assembly stored in the box body, and the T cell sorting magnetic bead assembly at least comprises a cell centrifugal material, a reagent, a cell sorting material and consumables, a plurality of grooves are formed in the box body and used for containing cell centrifugal materials, reagents, cell sorting materials and consumables respectively, triggers which can be triggered when objects in the grooves are taken out are correspondingly arranged in the grooves for containing the cell centrifugal materials, the reagents and the cell sorting materials respectively, and a power source, a control circuit and an alarm device are further arranged in the box body. The control circuit is respectively connected with the power supply, the alarm device and the plurality of triggers, and the control circuit is set to control the alarm device to give an alarm when the triggers are not triggered according to the preset sequence. And step prompting can be performed on T cell sorting, so that the T cell sorting is simpler, and experiment errors are effectively avoided.

Description

Technical Field

[0001] The utility model relates to the field of material boxes, in particular to a T cell sorting magnetic bead assembly. Background Art

[0002] The commonly used T cell sorting method uses immunomagnetic cell sorting, which requires the functionalization of the magnetic bead surface, the binding of cells and magnetic beads, the separation of target cells and non-target cells, etc., and multiple processes need to be completed in each step. The operation is very cumbersome. For novices, it is difficult to get started and often prone to errors; for experienced people, sometimes mistakes are also easy to make due to negligence. Utility Model Content

[0003] In view of the above-mentioned deficiencies in the current T cell sorting methods, the utility model provides a T cell sorting magnetic bead assembly, which can provide step prompts for T cell sorting, making T cell sorting simpler and effectively avoiding experimental errors.

[0004] In order to achieve the above-mentioned purpose, the embodiment of the utility model adopts the following technical scheme:

[0005] A T cell sorting magnetic bead assembly comprises a box body and a T cell sorting magnetic bead assembly stored in the box body, wherein the T cell sorting magnetic bead assembly comprises at least a cell centrifugal material, a reagent, a cell sorting material and consumables, wherein a plurality of grooves are provided in the box body for placing the cell centrifugal material, the reagent, the cell sorting material and the consumables respectively, wherein triggers which are triggered when the objects in the grooves are taken out are respectively provided in the grooves, wherein a power supply, a control circuit and an alarm device are also provided in the box body, wherein the control circuit is respectively connected to the power supply, the alarm device and the plurality of triggers, and wherein the control circuit is configured to control the alarm device to issue an alarm when the triggers are not triggered in a preset sequence.

[0006] According to one aspect of the utility model, the cell centrifugation material is located at the leftmost side of the box body, the cell sorting material is located at the rightmost side of the box body, the reagent is located between the cell centrifugation material and the cell sorting material, and the consumables are located behind the reagent.

[0007] According to one aspect of the present invention, the preset sequence is to sequentially take cell centrifugation material, reagent, and cell sorting material.

[0008] According to one aspect of the utility model, the alarm device is an audible and visual alarm.

[0009] According to one aspect of the utility model, the cell centrifugal material includes at least one centrifuge tube, and the centrifuge tube is placed in a corresponding groove.

[0010] According to one aspect of the utility model, the cell sorting material includes at least two sorting columns, the sorting columns are filled with reserved reagents, and the sorting columns are respectively placed in corresponding grooves.

[0011] According to one aspect of the utility model, the reagents include at least two incubation reagents placed in a tubular object and at least two column reagents placed in a tubular object, the incubation reagents are located on the left side of the column reagents, the incubation reagents are arranged in sequence from top to bottom and are respectively placed in corresponding grooves, and the column reagents are arranged in sequence from top to bottom and are respectively placed in corresponding grooves.

[0012] According to one aspect of the utility model, the consumables include a Pasteur pipette and a gun tip, and the Pasteur pipette and the gun tip are respectively placed in corresponding grooves.

[0013] According to one aspect of the present invention, the sensor is a pressure sensor and / or a light sensor.

[0014] According to one aspect of the utility model, a label for indicating a preset sequence is provided on the edge of the groove.

[0015] According to one aspect of the utility model, a cover body is provided at the upper end of the box body, and the cover body is hinged to the box body.

[0016] According to one aspect of the utility model, it also includes a packaging box (10), on which a display screen is provided, and the display screen is connected to the control circuit.

[0017] The advantages of the implementation of the utility model are as follows: when a user takes an object from a groove, after the object is taken away, a trigger transmits data to a control circuit, and the control circuit determines whether the taking order is correct. When the object in the groove is not taken in the prescribed order, the control circuit controls an alarm device to send out an alarm to remind the user, thereby effectively preventing the user from making operating errors. A display screen is provided on the packaging box, and relevant product information, such as article number, batch number, production date, expiration date, instruction manual information, etc., can be displayed on the display screen, and the object that should be taken next can also be indicated through the display screen. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 This is a schematic diagram of the appearance of the utility model;

[0020] Figure 2This is a first structural schematic diagram of the utility model;

[0021] Figure 3 This is a second structural schematic diagram of the utility model.

[0022] 1. Box body; 2. Cell centrifugation material; 3. Reagents; 4. Cell sorting material; 5. Consumables; 6. Trigger; 7. Power supply; 8. Control circuit; 9. Label; 10. Packaging box; 11. Display screen; 12. Alarm device. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Embodiment 1:

[0025] T lymphocytes, abbreviated as T cells, are derived from lymphoid stem cells derived from the bone marrow. After differentiation and maturation in the thymus, they are distributed to immune organs and tissues throughout the body through lymphatic and blood circulation to exert immune functions.

[0026] T cells are quite complex, heterogeneous, and constantly updated in the body. They can exist at different developmental stages or functional subgroups at the same time, but the classification principles and nomenclature are rather confusing and have not yet been unified. According to their different functions in the immune response, T cells can be divided into several subgroups: helper T cells, suppressor T cells, effector T cells, cytotoxic T cells, delayed allergic reaction T cells, primitive or natural T cells, and memory T cells.

[0027] Cell sorting is a technique that separates a specific cell subpopulation from a mixed cell sample based on the characteristics of the cell, and is an important topic in cytological research. When a specific cell needs to be analyzed for function, such as detecting cell molecules through ELISA analysis of cell culture supernatant, detecting cell function through cell co-culture, etc., the premise is to obtain high-purity target cells.

[0028] like Figure 1 , Figure 2 , Figure 3As shown, a T cell sorting magnetic bead assembly comprises a box body 1 and a T cell sorting magnetic bead assembly stored in the box body 1, wherein the T cell sorting magnetic bead assembly comprises at least a cell centrifugal material 2, a reagent 3, a cell sorting material 4 and a consumable 5, a plurality of grooves are provided in the box body 1 for placing the cell centrifugal material 2, the reagent 3, the cell sorting material 4 and the consumable 5 respectively, and triggers 6 which are triggered when the objects in the grooves are taken out are respectively provided in the grooves for placing the cell centrifugal material 2, the reagent 3 and the cell sorting material 4, a power supply 7, a control circuit 8 and an alarm device 12 are also provided in the box body 1, and the control circuit 8 is respectively connected to the power supply 7, the alarm device 12 and the plurality of triggers 6, and the control circuit 8 is configured to control the alarm device 12 to issue an alarm when the trigger 6 is not triggered in a preset order. In this embodiment, the preset order is to take the cell centrifugal material 2, the reagent 3, and the cell sorting material 4 in sequence. When in use, the user takes the object in the groove. When the object is taken away, the trigger 6 transmits data to the control circuit 8, and the control circuit 8 determines whether the taking order is correct. When the object in the groove is not taken in the prescribed order, the control circuit 8 controls the alarm device 12 to issue an alarm to remind the user, effectively preventing the user from making mistakes in operation. For example, the first step should be to take the cell centrifugal material 2. When the user takes the object of other steps, the trigger 6 will transmit data to the control circuit 8, and the control circuit 8 will control the alarm device 12 to issue an alarm to remind the user that the operation is wrong. After the user puts the cell centrifugal material 2 back to the corresponding groove, the alarm device 12 stops the alarm.

[0029] In actual application, the cell centrifugal material 2 is located at the leftmost side of the box body 1, the cell sorting material 4 is located at the rightmost side of the box body 1, the reagent 3 is located between the cell centrifugal material 2 and the cell sorting material 4, and the consumable 5 is located behind the reagent 3. Placing the cell centrifugal material 2 and the cell sorting material 4, which are larger in volume, on both sides is convenient for users to take, and placing the consumable 5 and the reagent 3, which are smaller in volume, in the middle area makes it easier to find the required objects when the consumable 5 and the reagent 3 are placed together.

[0030] In practical application, the warning device 12 is a sound and light alarm. When in use, the sound and light alarm will emit colored light and voice to warn.

[0031] In this embodiment, the sound and light alarm can be the Likean sound and light alarm AD-103.

[0032] In this embodiment, the control circuit 8 is a circuit control chip, and generally, the circuit control chip AIP1628 can be used.

[0033] In practical applications, the cell centrifugal material 2 includes at least one centrifugal tube, which is placed in a corresponding groove.

[0034] In this embodiment, the capacity of the centrifuge tube is 50 ml.

[0035] In actual application, reagent 3 includes at least two incubation reagents placed in a tubular object and at least two column reagents placed in a tubular object. The incubation reagents are located on the left side of the column reagents. The incubation reagents are arranged in sequence from top to bottom and placed in corresponding grooves respectively. The column reagents are arranged in sequence from top to bottom and placed in corresponding grooves respectively.

[0036] In this embodiment, the incubation reagent includes incubation reagent I and incubation reagent II, incubation reagent I contains 5ml 40μL of 2% human AB type serum, 3ml 1mM EDTA buffer, and 1ml 10μL of T cell biotin; incubation reagent II contains 5ml 30μL of 2% human AB type serum, 3ml 1mM EDTA buffer, and 1ml 20μL of T cell biotin.

[0037] In this embodiment, the column reagents include column reagent I and column reagent II, column reagent 3I contains 5ml 40μL of 2% human AB type serum, 1ml 1mM EDTA buffer; column reagent 3II contains 5ml 30μL of 2% human AB type serum, 1ml 1mM EDTA buffer.

[0038] In practical applications, the cell sorting material 4 includes at least two sorting columns, which are filled with reserved reagents and are placed in corresponding grooves. The sorting columns are used to collect T cells, and the sorting columns already contain reagents 3, which can omit the pretreatment step and improve the efficiency of T cell sorting.

[0039] In this embodiment, the sorting column includes sorting column I and sorting column II, the reserved reagent in sorting column I includes 3ml40μL of 2% human AB type serum, 1ml 1mM EDTA buffer; the reserved reagent in sorting column II includes 3ml 40μL of 2% human AB type serum, 1ml 1mM EDTA buffer.

[0040] In practical applications, the consumables 5 include a Pasteur pipette and a gun tip, which are placed in corresponding grooves, respectively. The Pasteur pipette and the gun tip are disposable products, and are used as consumables 5 for T cell sorting to absorb liquid, perform cell tests, and the like.

[0041] In practical applications, the sensor is a pressure sensor and / or a light sensor. When the pressure sensor or the light sensor senses that the object in the groove is removed, it transmits data to the control circuit 8.

[0042] In this embodiment, the pressure sensor may be a LSB206 S strain force sensor, and the optical sensor may be an EE-SX1340 transmissive micro photoelectric sensor.

[0043] In practical applications, a label 9 for indicating a preset sequence is provided on the edge of the groove, which is convenient for indicating the order in which the materials are used and reducing the probability of errors.

[0044] In practical applications, a cover is provided at the upper end of the box body 1, and the cover is hinged to the box body 1 to facilitate opening and closing of the cover.

[0045] In practical applications, the packaging box 10 is also included, and a display screen 11 is provided on the packaging box 10, and the display screen 11 is connected to the control circuit 8. The display screen 11 can display relevant information of the product, such as the article number, batch number, production date, expiration date, instruction manual information, etc., and can also indicate the object to be taken next through the display screen 1111. The packaging box 10 can also be recycled for multiple times, and the display screen 11 is modified accordingly according to the different placement of the objects in the groove, so that the utility model can be applied to the sorting of multiple cells.

[0046] In summary, the method of using the utility model is:

[0047] (1) PBMC / BMMC counting: Place the counted samples in a centrifuge tube and centrifuge at 1600 rpm for 6 minutes, and discard the supernatant.

[0048] (2) Resuspend 1×10 7 Incubate cells at 2-8°C in the dark for 5 minutes.

[0049] (3) Add Incubation Reagent II and incubate at 2-8°C in the dark for 10 minutes.

[0050] (4) Prepare separation column I and place it in a suitable MACS magnetic field separator.

[0051] (5) Add the incubated cells to the sorting column I, collect the T cells flowing down, wash the column with column reagent I, and collect the T cells flowing down.

[0052] (6) The collected cells are mixed, passed through the sorting column II for a second time, the column is washed with column reagent II, the T cells flowing down are collected, mixed, stained, and CD3 purity is detected by flow cytometry.

[0053] The above are only specific implementations of the utility model, but the protection scope of the utility model is not limited thereto. Any changes or substitutions that can be easily thought of by any technician familiar with the technical field within the technical scope disclosed in the utility model should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model shall be based on the protection scope of the claims.

Claims

1. A T cell sorting magnetic bead assembly, comprising a box body (1) and a T cell sorting magnetic bead assembly stored in the box body (1), wherein the T cell sorting magnetic bead assembly at least comprises a cell centrifugal material (2), a reagent (3), a cell sorting material (4) and a consumable (5), characterized in that: The box body (1) is provided with a plurality of grooves for placing cell centrifugal materials (2), reagents (3), cell sorting materials (4) and consumables (5) respectively. The grooves for placing the cell centrifugal materials (2), reagents (3) and cell sorting materials (4) are respectively provided with triggers (6) which are triggered when the objects in the grooves are taken out. The box body (1) is also provided with a power supply (7), a control circuit (8) and an alarm device (12). The control circuit (8) is respectively connected to the power supply (7), the alarm device (12) and the plurality of triggers (6), and the control circuit (8) is configured to control the alarm device (12) to issue an alarm when the triggers (6) are not triggered in a preset sequence.

2. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: The cell centrifugal material (2) is located at the leftmost side of the box body (1), the cell sorting material (4) is located at the rightmost side of the box body (1), the reagent (3) is located between the cell centrifugal material (2) and the cell sorting material (4), and the consumable (5) is located behind the reagent (3).

3. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: The preset sequence is to sequentially take the cell centrifugation material (2), the reagent (3), and the cell sorting material (4).

4. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: The warning device (12) is an audible and visual alarm.

5. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: The cell centrifugal material (2) comprises at least one centrifugal tube, which is placed in a corresponding groove.

6. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: The cell sorting material (4) comprises at least two sorting columns, wherein the sorting columns are filled with a reserved reagent (3), and the sorting columns are respectively placed in corresponding grooves.

7. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: The reagent (3) includes at least two incubation reagents placed in a tubular object and at least two column reagents placed in a tubular object, wherein the incubation reagents are located on the left side of the column reagents, the incubation reagents are arranged in sequence from top to bottom and are respectively placed in corresponding grooves, and the column reagents are arranged in sequence from top to bottom and are respectively placed in corresponding grooves.

8. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: The consumables (5) include a Pasteur pipette and a gun tip, and the Pasteur pipette and the gun tip are respectively placed in corresponding grooves.

9. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: The trigger (6) is a pressure sensor and / or a light sensor.

10. The T cell sorting magnetic bead assembly according to any one of claims 1 to 7, characterized in that: The edge of the groove is provided with a label (9) for indicating a preset sequence.

11. The T cell sorting magnetic bead assembly according to claim 1, characterized in that: A cover body is provided at the upper end of the box body (1), and the cover body is hinged to the box body (1).

12. The T cell sorting magnetic bead assembly according to claim 9, characterized in that: It also comprises a packaging box (10), wherein the packaging box (10) is provided with a display screen (11), and the display screen (11) is connected to the control circuit (8).

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