2 results about "Zanthoxylum simulans" patented technology

This invention belongs to the field of Sichuan pepper cultivation technology, and particularly relates to a method and application for increasing the volatile oil content of Dingtan Sichuan pepper fruits. A method for increasing the volatile oil content of Dingtan Sichuan pepper fruits includes the following steps: pruning branches during the fruit harvesting period (mid-to-late July); layering and pruning branches in October to create an open-center tree shape; turning the soil once in June-July after pruning; applying organic fertilizer in July of the same year after pruning; applying compound fertilizer once in August and once in March-April of the following year, wherein the fertilization area extends horizontally 1.2-1.5 times beyond the tree canopy line and vertically 20-30 cm above the soil surface; maintaining the moisture content at 25%-30%; spraying with acetamiprid once in September and once in October; burning Sichuan pepper branches and leaves when the temperature is below 0℃; and controlling pests and diseases. This invention improves the metabolic activity of Sichuan pepper by improving light conditions and nutritional status, increasing the time shift of metabolism, and promoting the synthesis of volatile oils.

The present application relates to the technical field of pepper germplasm resource screening, and particularly relates to a DNA barcode for identifying the genetic relationship of pepper, a primer set and application thereof. The present application provides a DNA barcode and primer set for identifying pepper germplasm resources. The DNA barcode is obtained according to the SSR sequences of Zanthoxylum simulans, Zanthoxylum simulans and hybrid offspring. The barcode is applied to the identification of pepper germplasm resources after the correct amplification of the primer set is screened. The experimental results show that the DNA barcode and primer set can be combined in various forms to identify the genetic relationship of pepper, and the detection cycle is short, the operation is simple, the detection process is economical and saving, the identification result is accurate and reliable, and the repeatability is good. The present application overcomes the shortcomings of the traditional morphological identification method, such as inaccuracy, time-consuming and laboriousness, and plays an active role in the identification of the genetic relationship of high-quality pepper germplasm resources and genetic breeding. Meanwhile, the present application provides an effective method for the identification and protection of germplasm resources.