The invention discloses a traditional Chinese medicine composition for the treatment of bovine mastitis. The composition is prepared from 9 Chinese medicinal materials including radix bupleuri, Chinese violet, dandelion, wild chrysanthemum flower, forsythia, herba andrographis, pangolin, musk and toad venom. The composition contains a plurality of biological active ingredients including alkaloids, glycosides and organic acids, has the same source with the feed, easiness for absorption and no toxic or side effect, has the effects of clearing heat, detoxifying, resisting bacteria, relieving inflammation, stimulating the menstrual flow, invigorating blood circulation, subsiding swelling, relieving carbuncle, activating channels and promoting lactation, and can promote animal growth and development and enhance the immunity of animals.