Method for determining physiological state of microbial community and method for treating drainage water

A technology of microbial community and physiological state, applied in biological water/sewage treatment, biochemical equipment and methods, water treatment parameter control, etc., can solve problems such as difficulties, and achieve the effect of stable drainage treatment

Inactive Publication Date: 2011-06-08
SEIKO PMC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, until now, an effective method for judging the physiological state of the microbial community that exists in the treatment tank has not been established quickly and accurately, and it is difficult to prevent abnormalities such as swelling.

Method used

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  • Method for determining physiological state of microbial community and method for treating drainage water
  • Method for determining physiological state of microbial community and method for treating drainage water
  • Method for determining physiological state of microbial community and method for treating drainage water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0160] [Improvement example of adding microbial agent to activated sludge system]

[0161] In an activated sludge treatment facility for industrial wastewater, for the purpose of improving the bulking (SVI value) of activated sludge, the countermeasure of adding microbial agents is adopted based on the fluorescence microscope observation image of the microbial community constituting the sludge.

[0162] The volume load of the target activated sludge treatment equipment is 1.4kg-BOD / m 3 / day.

[0163] [Fluorescence staining of activated sludge]

[0164] The activated sludge sample obtained from the sampling of the aeration tank outlet of the activated sludge treatment equipment was double tested using the first fluorochrome PI (final concentration 1mg / L) and the second fluorochrome CFDA (final concentration 10mg / L). dyeing.

[0165] In addition, PI and CFDA used the following reagents:

[0166] · · PI (Propidium iodide, manufactured by Wako Pure Chemical Industries, Ltd., 1...

Embodiment 2

[0184] [Example of improvement based on shortening operation of solids residence time in activated sludge system]

[0185]In an activated sludge treatment facility for industrial wastewater, in order to improve the transparency of the treated water, the operation of shortening the residence time of solids is carried out based on the fluorescence microscope observation image of the microbial community constituting the sludge.

[0186] In the embodiment, the volume load of the activated sludge treatment equipment as the object is 1.2kg-BOD / m 3 / sky.

[0187] The double staining method of the activated sludge sample, the observation method, and the criteria for judging the physiological state of the sample were performed in the same manner as in the case of Example 1 above.

[0188] [Improve the agglutination of sludge-forming microbial communities by shortening the residence time of solids]

[0189] Fluorescence microscopy was performed on the activated sludge samples before t...

Embodiment 3

[0199] [Example of improvement in operation by adding microbial agents to the activated sludge system and shortening the residence time of solids]

[0200] In the activated sludge treatment equipment of industrial drainage, in order to improve the sedimentation rate of sludge and increase the COD reduction rate, based on the fluorescence microscope observation image of the microbial community that constitutes the sludge, adding microbial agents and shortening the solid retention time operation.

[0201] In the embodiment, the volume load of the activated sludge treatment equipment as the object is 2.2kg-BOD / m 3 / sky.

[0202] The double staining method of the activated sludge sample, the observation method, and the criteria for judging the physiological state of the sample were performed in the same manner as in the case of Example 1 above.

[0203] [Improvement by adding microbial agents and shortening the solid residence time]

[0204] Fluorescence microscopy was performe...

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PUM

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Abstract

Provided is a method for determining the physiological state of a microbial community whereby the physiological state of a microbial community present in a treatment tank of an apparatus for biologically treating drainage water can be quickly and accurately determined. Also provided is a method for treating drainage water with the use of the above-described method. A method for determining the physiological state of a microbial community comprising a step of dyeing a microbial community collected from a treatment tank of an apparatus for treating drainage water with a first fluorescent dye which binds to a nucleotide chain of the microorganisms and a second fluorescent dye which is decomposed with an enzyme in microbial cells and thus emits a fluorescence having a wavelength differing from that of the first fluorescent dye as described above, and a step of measuring, relating to the microbial community having been dyed as described above, the first fluorescence intensity (F1) originating from the first fluorescent dye as described above and the second fluorescence intensity (F2) originating from the second fluorescent dye as described above, wherein the preference level of the physiological state of the microbial community is determined based on the ratio (F1 / F2) of the measured values.

Description

technical field [0001] The present invention relates to a method for judging the physiological state of microbial flora that can quickly and accurately judge the physiological state of microbial flora existing in a treatment tank of biological treatment equipment for drainage. The present invention also relates to a wastewater treatment method capable of stably performing wastewater treatment while maintaining and controlling the physiological state of the microbial community based on the physiological state of the microbial community judged by the determination method. The present invention can be applied to treatment tanks of various drainage treatment equipment such as an aeration tank of a drainage treatment equipment using an activated sludge method or a reaction tank of a drainage treatment equipment using a biofilm method. Background technique [0002] In the wastewater treatment facility using the activated sludge method, while supplying air to the treatment tank (ae...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C02F3/12
CPCC02F2209/36G01N33/569C02F2209/003C02F3/348G01N33/582C02F2103/001C12Q1/04
Inventor 五十岚亮二山本琢二
Owner SEIKO PMC CORP
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