Method for stabilizing labeled antibody

A labeled antibody and stabilization technology, applied in the field of labeled antibody stabilization, can solve problems such as long-term storage difficulties

Inactive Publication Date: 2011-11-23
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the activity of enzymes and labeled antibodies is easily affected by temperature, chemical substances, etc., and it is difficult to store them for a long time while maintaining the activity of enzymes or labeled antibodies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0048] Still another embodiment of the present invention relates to a commercially available package, which is a liquid reagent containing a labeled antibody containing a labeled antibody and a stabilizer for stabilizing the labeled antibody in the liquid reagent, and a package for packaging the labeled antibody liquid reagent. A commercial package for a container, wherein the stabilizer is at least one of amino acid and its derivatives. "Packaging for commercial use" in this specification refers to, for example, packing, filling, or storing a liquid reagent of a labeled antibody containing a labeled antibody and the above-mentioned stabilizer in a container in a state that can be distributed or that can be traded. , for example, can be called product or distribution packaging. Since the commercially available package of the present invention contains the labeled antibody reagent, it can be used, for example, as an analytical reagent for molecular biology or biochemistry, an i...

Embodiment 1～5、 comparative example 1

[0053]

[0054] An alkaline phosphatase-labeled anti-LH mouse monoclonal antibody was used as a labeled antibody, and the stability of the labeled antibody was evaluated by an accelerated storage stability test.

[0055] [labeled antibody]

[0056] The alkaline phosphatase-labeled anti-LH mouse monoclonal antibody was prepared by labeling an anti-LH mouse monoclonal antibody (manufactured by Fitzgerald Corporation) with an alkaline phosphatase labeling kit (manufactured by Dojin Chemical).

[0057] [labeled antibody solution]

[0058] The obtained alkaline phosphatase-labeled anti-LH mouse monoclonal antibody was diluted with the reagent of the following composition to obtain an alkaline phosphatase-labeled antibody solution (antibody final concentration: 0.7 μg / mL). Among them, the stabilizers shown in Table 1 below were used as stabilizers.

[0059] (Reagent Composition)

[0060] 50mM Tris

[0061] 150mM NaCl

[0062] 1mM MgCl 2

[0063] 0.025mM ZnCl 2

[0064] 0....

Embodiment 6～10、 comparative example 2

[0109]

[0110] Alkaline phosphatase-labeled anti-FSH mouse monoclonal antibody was used as a labeled antibody, and the stability of the labeled antibody was evaluated by an accelerated storage stability test.

[0111] [labeled antibody]

[0112] The alkaline phosphatase-labeled anti-FSH mouse monoclonal antibody was prepared by labeling the anti-FSH mouse monoclonal antibody (manufactured by Roche) with an alkaline phosphatase labeling kit (manufactured by Dojin Chemical).

[0113] [labeled antibody solution]

[0114] The obtained alkaline phosphatase-labeled anti-FSH mouse monoclonal antibody was diluted with the reagent of the following composition to obtain an alkaline phosphatase-labeled antibody solution (antibody final concentration: 0.4 μg / m1). Among them, the stabilizers shown in Table 4 below were used as stabilizers.

[0115] (Reagent Composition)

[0116] 50mM Tris

[0117] 150mM NaCl

[0118] 1mM MgCl 2

[0119] 0.05mM ZnCl 2

[0120] 0.1%BSA

[0121] ...

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Abstract

The present invention relates to a method for stabilizing a labeled antibody in a solution, in which the labeled antibody is stabilized by allowing the labeled antibody to be present together with at least one of amino acid and a derivative thereof in the solution.

Description

technical field [0001] The present invention relates to a method for stabilizing labeled antibodies in solution. Background technique [0002] Proteins such as enzymes, labeled antibodies, and primary antibodies are widely used in clinical examinations, biochemistry, and other fields. However, the activity of enzymes and labeled antibodies is easily affected by temperature, chemical substances, etc., and it is difficult to store them for a long time while maintaining the activity of enzymes or labeled antibodies. Therefore, various methods for stably maintaining the activities of these proteins have been proposed. [0003] Enzymes and labeled antibodies are provided in the form of liquid reagents dissolved in a buffer or freeze-dried freeze-dried reagents, and various stabilization methods have been proposed for each form. In the case of a liquid reagent, for example, a method of including crystallin in a cholesterol dehydrogenase solution (for example, Japanese Patent No....

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C07K1/00G01N33/53
CPCA61K39/39591
Inventor高木美佳子小野绘梨子
OwnerARKRAY INC