Method for determining effective component content in chenopodium ambrosioides volatile oil
A technology for the volatile oil and active ingredients of Nepeta nepeta, which is applied in the directions of measuring devices, material separation, and analysis of materials, etc., can solve the problems of unreported content determination methods, and achieve the effect of avoiding unstable measurement results
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[0054] 1. Instruments and materials
[0055] American Agilent 1100 high performance liquid chromatography (ultraviolet detector, binary pump, autosampler, column thermostat).
[0056]Ascaridin reference substance (self-made, proved to be ascaridin by structure, the purity is 90.0% by HPLC analysis), p-cymene reference substance (St.Louis, USA, purity is 99.0%), α-terpinene reference substance Product (self-made, confirmed to be α-terpinene by structure, the purity of HPLC analysis is 92.37%), di-pure water (self-made), acetonitrile (Merck Company) is chromatographically pure, and other reagents are analytically pure.
[0057] The volatile oil of Nepeta chinensis (identified as Chenopodium ambrosioides L. by Professor Wang Zhenyue, Department of Traditional Chinese Medicine Resources and Development, School of Pharmacy, Heilongjiang University of Traditional Chinese Medicine) was provided by Shaanxi Tasly Plant Pharmaceutical Co., Ltd.
[0058] 2. Methods and results
[0059]...
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