Rooting method of subprostrate sophora root tissue culture seedling leaf stalks

Abstract

The invention relates to a rooting method of subprostrate sophora root tissue culture seedling leaf stalks. The method comprises the following steps that: (1) subprostrate sophora root tissue culture seedlings are inoculated into a Murashige and Skoog (MS) seedling strengthening culture medium for strengthening the seedling so that tissue culture seedling leaves are spread to obtain the complete high-lignification-degree compound leaves with leaf stalks; and (2) the compound leaves with leaf stalks are placed into an MS rooting culture medium to be cultured and inducted for rooting. When the method provided by the invention is adopted, a large number of root materials are obtained from the subprostrate sophora root tissue culture seedling leaf stalks, and a novel path is provided for subprostrate sophora root medical material resources and active ingredient extraction material sources.

Description

technical field [0001] The invention relates to a method for rooting the petiole of tissue-cultured seedlings, in particular to a method for rooting the petiole of tissue-cultured seedlings of broad bean roots. Background technique [0002] Broad bean root, also known as mountain bean root and southern bean root, is the dried root and rhizome of the leguminous plant Sophora tonkinensis Gapnep. It is a famous authentic medicinal material in Guangxi and a commonly used traditional Chinese medicine. collected. It is mainly distributed in Guangxi, and it is also produced in Yunnan and Guizhou, but the output is small. The medicinal material of broad bean root is bitter, cold and poisonous. It has the functions of clearing heat and detoxifying, reducing swelling and relieving pain, etc. It can treat sore throat, sore throat, sore throat, swollen gums, hot cough, hepatitis, constipation, jaundice, diarrhea, hemorrhoids , bald sores, scabies and mosquitoes, insects, dog bites and...

AI Technical Summary

Problems solved by technology

However, the geographical distribution of the broad bean root is quite limited, and it often grows sporadically in the crevices of rocky mountains; under natural conditions, the broad bean root is severely damaged by diseases and insect pests during the flowering and pod stage, and the seeds are easy to fall off naturally when they mature, and the seeds are vigorous after harvesting. Easy to decline, these often result in low seed yield and poor quality, making it difficult to achieve large-scale cultivation in production

However, in order to meet market demand and obtain economic benefits, people excavated a large number of broad bean root resources, causing wild broad bean root resources to be on the verge of depletion

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