Rooting method of subprostrate sophora root tissue culture seedling leaf stalks

A petiole and tissue culture technology is applied to the rooting field of the petioles of the tissue culture seedlings of broad bean root, and can solve the problems of serious pests and diseases, easy decline of vigor, low seed yield and the like.

CN102499083BActive Publication Date: 2013-06-12GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2013-06-12

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Abstract

The invention relates to a rooting method of subprostrate sophora root tissue culture seedling leaf stalks. The method comprises the following steps that: (1) subprostrate sophora root tissue culture seedlings are inoculated into a Murashige and Skoog (MS) seedling strengthening culture medium for strengthening the seedling so that tissue culture seedling leaves are spread to obtain the complete high-lignification-degree compound leaves with leaf stalks; and (2) the compound leaves with leaf stalks are placed into an MS rooting culture medium to be cultured and inducted for rooting. When the method provided by the invention is adopted, a large number of root materials are obtained from the subprostrate sophora root tissue culture seedling leaf stalks, and a novel path is provided for subprostrate sophora root medical material resources and active ingredient extraction material sources.
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Description

technical field

[0001] The invention relates to a method for rooting the petiole of tissue-cultured seedlings, in particular to a method for rooting the petiole of tissue-cultured seedlings of broad bean roots. Background technique

[0002] Broad bean root, also known as mountain bean root and southern bean root, is the dried root and rhizome of the leguminous plant Sophora tonkinensis Gapnep. It is a famous authentic medicinal material in Guangxi and a commonly used traditional Chinese medicine. collected. It is mainly distributed in Guangxi, and it is also produced in Yunnan and Guizhou, but the output is small. The medicinal material of broad bean root is bitter, cold and poisonous. It has the functions of clearing heat and detoxifying, reducing swelling and relieving pain, etc. It can treat sore throat, sore throat, sore throat, swollen gums, hot cough, hepatitis, constipation, jaundice, diarrhea, hemorrhoids , bald sores, scabies and mosquitoes, insects, dog bites and...

Examples

Embodiment Construction

[0012] The technical solutions of the present invention will be further described below in conjunction with the examples.

[0013] A method for rooting the petiole of broad bean root tissue culture seedling, comprising the following steps:

[0014] (1) Cultivation of strong seedlings of group-cultured clustered shoots: Take broad bean root tissue-cultured clustered shoots and inoculate them into MS strong seedling medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 12-14 hours / Cultivate for 30 days under the condition of 1 day, and the leaves will expand after the lignification of the clustered buds, and the compound leaves with stalks with three leaves will be obtained, wherein the MS strong seedling medium contains 1.0mg / L of 6-BA and 0.1mg / L of NAA , the IBA of 0.5mg / L, the agar of 30g / L sucrose and 3.4g / L, the pH value of culture medium is 5.8;

[0015] (2) rooting culture of tissue culture seedling petiole: the compound le...