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Kit for identifying fruit flies and special primers therefor

A fruit fly and reagent technology, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as the inability to meet the needs of port quarantine and domestic fruit fly monitoring, and ensure the accuracy, practicability and protection of the technology. Agricultural production and ecological security, the effect of strong technical versatility

Inactive Publication Date: 2013-10-09
CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the traditional identification technology of fruit fly species based on morphological characteristics can no longer meet the needs of port quarantine and domestic fruit fly monitoring.

Method used

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  • Kit for identifying fruit flies and special primers therefor
  • Kit for identifying fruit flies and special primers therefor
  • Kit for identifying fruit flies and special primers therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Embodiment 1, the design of primer

[0091] 1. Acquisition of fruit fly DNA barcodes

[0092] The Barcode public sequences of Bactrocera dorsalis, Bactrocera capsicum, Bactrocera guava, Bactrocera melon and Bactrocera australis can be obtained by querying in the BOLD and GenBank systems. The public sequence numbers of the template sequences are Bactrocera dorsalis (DQ116269), Bactrocera capsicum (DQ116296), Bactrocera guava (DQ116264), Bactrocera melon (DQ116242), and Bactrocera japonica (GU323773.1).

[0093] For the target gene sequence that was not retrieved in the database, use the universal primers LCO-1490 (5'-GGTCAACAAATCATAAAGATATTG-3') and HCO-2198 (5'-TAAACTTCAGGGTGACCAAAAAAATCA-3') to carry out the extraction of the fruit fly genomic DNA target fragment amplification.

[0094] 2. Design of primers

[0095] Use DNAMAN software to compare and analyze the DNA barcodes of the target species of fruit flies. According to the analysis results, artificially design...

Embodiment 2

[0100] Embodiment 2, identification of fruit flies

[0101] Bactrocera dorsalis, Bactrocera capsicum, Bactrocera guava, Bactrocera melon, Bactrocera melon, Bactrocera australis, Bactrocera cerevisiae, Bactrocera nigella, Bactrocera cucurbita, Bactrocera citrus were collected from different locations Bacteria and Bacteria dorsalis, of which,

[0102] Bactrocera (Bactrocera) dorsalis (Hendel) is recorded in Zhang Bin, Liu Yinghong, Zhao Lanlan, Zhou Xu. Research progress of Bactrocera dorsalis, China Agricultural Science Bulletin, 2008, 24 (11): 391-397, available to the public Obtained from China Agricultural University.

[0103] Bactrocera (Bactrocera) latifrons (Hendel) was recorded in Huang Xing, Huang Kehui. Quarantine Pests - Morphology, Hazards and Quarantine Countermeasures of Bactrocera capsicum, Wuyi Science, 2009, 25: 21-23., the public can from obtained from China Agricultural University.

[0104] Guava fruit fly Bactrocera (Bactrocera) correcta (Bezzi) is recorde...

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Abstract

The invention discloses a kit for identifying fruit flies and special primers therefor. The primers provided in the invention comprise eleven primer pairs from a primer pair 1 to a primer pair 11. Experiments of the invention prove that a method in the invention has the following advantages: researches in the invention combine the monitoring case, port interception information, host information and the like, main economic flies in Chinese mainland and Taiwan area are contained, and the kind representativeness of the contained flies is strong, so the identification result accuracy and the technological practicality are ensured.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for identifying fruit flies and special primers thereof. Background technique [0002] Tephritid flies belong to Diptera (Diptera) Tephritidae (Tephritidae), widely distributed all over the world, mostly occur in tropical and subtropical regions. There are about 500 genera and 4,500 species of Tephritidae, and there are more than 150 species of fruit flies with important economic significance. Some fruit flies can cause devastating disasters to fruit production. There are five genera of fruit flies of economic importance in the world, namely Anastrepha, Bactrocera, Ceratitis, Dacus and Around the genus Rhagoletis. The results of the national fruit fly monitoring survey show that the economically important fruit flies that occur in mainland China and Taiwan are mainly Bactrocera species. [0003] According to my country's newly revised list of imported plant quarantine pests,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 李志红姜帆吴佳教胡学难刘海军顾渝娟
Owner CHINA AGRI UNIV
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