S-segment loop-mediated isothermal amplification rapid detection kit and method for server fever with thrombocytopenia syndrome bunyavirus

Abstract

The invention discloses an S-segment loop-mediated isothermal amplification rapid detection kit for server fever with thrombocytopenia syndrome bunyavirus, which is composed of the following components of: (1) 2.5 microliters of 10X amplification reaction solution; (2) 2 microliters of dNTP with the concentration of 25 mmol / l; (3) 5 pmol of F3 / B3 outer primers, respectively; 40 pmol of FIP / BIP inner primers, respectively; 20 pmol of LF / LB annular primers, respectively; (4) 1 microliter of BstDNA polymerase; (5) 1 microliter of reverse transcriptase; (6) 1 microliter of fluorescent dye; and (7) ddH2O. When the kit is used, RNA (Ribonucleic Acid) of a person to be detected is added; after amplification, the color change of a reaction system is observed, so as to judge a detection result. The detection kit and the detection method, disclosed by the invention, have the advantages of low detection cost, convenience in operation and high detection speed, realize high specificity and high flexibility which is the same as PCR (Polymerase Chain Reaction) and provide scientific bases for diagnosing and treating the server fever with thrombocytopenia syndrome in time.

Description

technical field [0001] The invention relates to a rapid detection kit for fever with thrombocytopenia syndrome bunyavirus S segment loop-mediated isothermal amplification, its use method, and its application in the detection of bunyavirus. Background technique [0002] Severe fever with thrombocytopenia syndrome bunyavirus (Severe fever with thrombocytopenia syndrome bunyavirus, SFTSV) has been confirmed as the cause of fever with thrombocytopenia syndrome that has been highly sporadic in Hubei, Henan, Jiangsu, Zhejiang and other regions since 2004. major pathogens. According to relevant research by the National Center for Disease Control and Prevention, the virus belongs to a new virus belonging to the genus Phleboviridae of the family Bunyaviridae. [0003] Similar to other Phleboviruses of the Bunyaviridae family, the genome of SFTSV consists of three single-stranded negative-sense RNA segments, large (L), medium (M), and small (S). The ends of each fragment are complem...

AI Technical Summary

Problems solved by technology

However, PCR detection technology requires expensive equipment and reagents, and fluorescent quantitative PCR requires expensive probes and complicated operation steps, and has high requirements for personnel's operation level, which is not suitable for rural, mountainous, and county-level applications. Health centers and other front-line adoption

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