Anti-rice black-streaked dwarf virus field inoculation identification method for rice varieties

Abstract

The invention provides an anti-rice black-streaked dwarf virus field inoculation identification method for rice varieties, and belongs to the technical field of agricultural science. A piece of rice field with certain conditions is selected as an identification nursery, different rice varieties are sown in special stages, it is identified that virus transmission interbody small brown rice planthoppers in the nursery have certain virus carried rate and population density after wheat harvest, and after rice grows to a specific growth period, the anti-rice black-streaked dwarf virus level of the rice varieties is evaluated according to the severity degree of disease. With the method, anti-rice black-streaked dwarf virus identification can be performed on a large number of rice varieties at a time, and the defects that the manual identification scale in a laboratory and the identification variety number are small are overcome. The identification method can be applied to the multiple fields of screening of anti-rice black-streaked dwarf virus rice varieties, variety resistance evaluation, resistant variety breeding, researching on the law of genetics of resistance and the like.

Description

technical field [0001] The invention relates to a field identification method for the resistance of rice varieties to black-streaked dwarf disease, belonging to the field of agricultural science and technology. Background technique [0002] Rice black-streaked dwarf disease is a viral disease caused by rice black-streaked dwarf virus (RBSDV), which is mainly transmitted by persistent non-egg-like way by the small planthopper. In recent years, with changes in farming systems and cultivation methods, warming of the winter climate, and large-scale promotion of susceptible varieties, the incidence of the virus-transmitting agent SBPH has increased. It occurred on a large scale in Fujian and quickly rose to become the most important viral disease in production. According to statistics from the Plant Protection Station of Jiangsu Province, as of 2007, the disease had seriously occurred in Yancheng, Lianyungang, Taizhou, Huai'an, Yangzhou and Xuzhou along the coast of the province...

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Problems solved by technology

In addition, the manual identification steps in the laboratory are relatively cumbersome, and the identification space is limited. Large-scale variety identification cannot be carried out. However, resource screening and resistance breeding often need to identify thousands of rice varieties or lines, and at the same time investigate the growth of these varieties in the field. This has greatly restricted the screening of rice resources for resistance to black-streaked dwarf and breeding for disease resistance. It is urgent to invent a field identification method that can be used for large-scale identification of black-streaked dwarf disease in rice. Provide basic research methods for resource excavation and screening, variety selection and genetic law research of stripe dwarf disease

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