Human H-FABP colloidal gold test paper and preparation method thereof

A technology for detecting test strips and colloidal gold, applied in the field of in vitro diagnostic medical testing, can solve the problems of high economic cost, unfavorable promotion and application of POCT, and high price of end products, and achieve the effects of promoting rapid release, shortening sample turnaround time, and improving labeling effect.

Active Publication Date: 2014-07-02
SHANGHAI KEHUA BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the main biological raw materials used in the research and development of myocardial products, that is, antibody pairs, generally use imported antibodies, and t

Method used

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  • Human H-FABP colloidal gold test paper and preparation method thereof
  • Human H-FABP colloidal gold test paper and preparation method thereof
  • Human H-FABP colloidal gold test paper and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the preparation of H-FABP colloidal gold detection test paper

[0047] In this embodiment, the buffer system solution involved in the preparation of the detection test paper of the present invention is prepared according to the following formula:

[0048]

[0049]

[0050]

[0051] Buffer solution 3: Tris-Hcl 10mM

[0052] Sucrose 5%

[0053] Buffer solution 4: Phosphate buffer 10mM

[0054] Tween-20 0.1%

[0055] The preparation method of H-FABP colloidal gold detection test paper comprises the steps:

[0056] Preparation of colloidal gold particles around 1.40nm:

[0057] (1) Add 200ml0.01% chloroauric acid solution in a 250ml Erlenmeyer flask, use a magnetic heating stirrer at 180r / min to stir and heat to boiling;

[0058] (2) keep boiling for 1min;

[0059] (3) Increase the rotating speed to 350r / min, and quickly add 2ml of 2% trisodium citrate solution to the side of the vortex;

[0060] (4) Continue to boil for...

Embodiment 2

[0080] Example 2: Detection time and sensitivity

[0081] 1. Prepare three different buffer solutions 1: ①, ② and ③, ① add 0.05% PEG-20000 in mass concentration, ② do not add PEG-20000, ③ add 0.5% trisodium citrate to prepare three different gold Label conjugate pad;

[0082] 2. Use high, medium and low-value quality control products with three different concentrations to test three different detection test papers, among which the concentrations of high, medium and low-value quality control levels are 120ng / ml, 30ng / ml, and 6ng / ml respectively , the results are shown in Table 1.

[0083] 3. Utilize the test paper KHB prepared according to the preparation method of Example 1 and the existing product A with a good reputation on the market to compare the detection time, and the results are shown in Table 2.

[0084] 4. Utilize a series of H-FABP quality control products diluted by gradient, detect respectively with the detection test paper of the present invention, the result s...

Embodiment 3

[0094] Embodiment 3: detection test paper analytical performance - specificity

[0095] 1. Prepare two different sample pad treatment solutions, namely buffer solution 2: ① without S16 and Tween-20; ② with 0.5% S16 and 0.05% Tween-20. Two different test papers ① and ② were prepared by treating the sample pad with the above two different treatment solutions.

[0096] 2. Compared with the existing marketed product A, a representative viscous serum sample (moderately positive) was used for detection, and the results are shown in Table 3.

[0097] Table 3 Effect of viscous samples on specificity

[0098]

5min

10min

15min

Test strip ①

+

++

+++

Test paper②

+

++

++

Existing product A

+

++

+++

[0099] Note: "-" means negative, "±" means gray area, "+" means weak positive, "++" means moderate positive, "+++" means strong positive.

[0100] 3. No cross-interference phenomenon was found in the test...

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Abstract

The invention relates to a preparation method of a piece of human H-FABP colloidal gold test paper. The preparation method comprises the steps of preparing colloidal gold particles, preparing gold labelling antibody, preparing a gold labelling combination mat, coating a nitrocellulose membrane, preparing a sample processing mat, assembling the test paper and the like. According to the preparation method of the human H-FABP colloidal gold test paper, by designing a buffer solution system in the preparation process of the test paper, the problems that the detection time is long, the specificity is poor, the cost is high, and the like in the existing test paper can be solved. The invention also discloses a piece of human H-FABP colloidal gold test paper and application thereof.

Description

【Technical field】 [0001] The invention relates to the field of in vitro diagnostic medical testing, in particular to a human heart-type fatty acid binding protein (Heart-type Fatty Acid Binding Protein, H-FABP) colloidal gold detection test paper and a preparation method thereof. 【Background technique】 [0002] In recent years, cardiovascular and cerebrovascular diseases, especially acute myocardial infarction (AMI) have become the killers with extremely high prevalence and the highest mortality rate among Chinese population. Within the "golden 6 hours" of treatment, patients can quickly enter the green treatment channel, which can greatly reduce the disability and mortality rates. Therefore, the accurate and rapid diagnosis of AMI has important medical significance. [0003] As a small molecule soluble protein biochemical marker for early myocardial diagnosis, H-FABP exists in the cytoplasm of myocardial cells and is abundant. When the myocardium is damaged, the integrity ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/544G01N33/532
CPCG01N33/532G01N33/558G01N33/6893G01N2800/324
Inventor 黄芳芳金巍李基
Owner SHANGHAI KEHUA BIO ENG
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