A method for continuous enzymatic hydrolysis of lignocellulose to increase sugar concentration

A technology of lignocellulose and sugar concentration, applied in biofuel, fermentation and other directions, can solve the problems of complex operation process, low overall concentration, and high consumption of enzymes, so as to improve the enzymatic hydrolysis rate, improve the efficiency of the enzymatic hydrolysis process, Reduce the effect of feedback inhibition

Active Publication Date: 2019-06-07
河南天冠纤维乙醇有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it consumes a lot of enzymes, the operation process is complicated, and the overall concentration is not high, so it is inconvenient to carry out continuously

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The present invention will be further described by taking the 50L enzymolysis tank as an example.

[0041] A method for continuous enzymatic hydrolysis of lignocellulose to increase sugar concentration. Multiple enzymatic hydrolysis tanks are started at the same time according to a certain feeding ratio. After the feeding is completed and enzymatic hydrolysis for a specific period of time, the materials are divided, and lignocellulose is added to the primary enzymatic hydrolysis tank. For hydrolytic enzymes, materials and water, only add materials to the secondary enzymatic hydrolysis tank to increase its solid content to the set value, and maintain a high concentration of continuous enzymatic hydrolysis, the following steps can be followed:

[0042] 1) Add 19.5L of tap water to the 50L enzymatic hydrolysis tanks M1 and M2 after the air disinfection treatment, start stirring, set the substrate concentration to 25% (w / v), and feed the water content into M1 and M2 respecti...

Embodiment 2

[0046] at 150m 3 The enzymolysis tank is taken as an example to further illustrate the present invention.

[0047] A method for continuous enzymatic hydrolysis of lignocellulose to increase sugar concentration. Multiple enzymatic hydrolysis tanks are started at the same time according to a certain feeding ratio. After the feeding is completed and enzymatic hydrolysis for a specific period of time, the materials are divided, and lignocellulose is added to the primary enzymatic hydrolysis tank. For hydrolytic enzymes, materials and water, only add materials to the secondary enzymatic hydrolysis tank to increase its solid content to the set value, and maintain a high concentration of continuous enzymatic hydrolysis, the following steps can be followed:

[0048] 1) 150m to the air after treatment 3 Add 58.5m of tap water to the enzymolysis tanks M1 and M2 respectively 3 , start stirring, set the substrate concentration to 25% (w / v), put 10 tons (1 / 6) of corn stalks with a water ...

Embodiment 3

[0052] at 150m 3 The enzymolysis tank is taken as an example to further illustrate the present invention.

[0053] A method for continuous enzymatic hydrolysis of lignocellulose to increase sugar concentration. Multiple enzymatic hydrolysis tanks are started at the same time according to a certain feeding ratio. After the feeding is completed and enzymatic hydrolysis for a specific period of time, the materials are divided, and lignocellulose is added to the primary enzymatic hydrolysis tank. For hydrolytic enzymes, materials and water, only add materials to the secondary enzymatic hydrolysis tank to increase its solid content to the set value, and maintain a high concentration of continuous enzymatic hydrolysis, the following steps can be followed:

[0054] 1) 150m to the air after treatment 3 Add 58.5m of tap water to the enzymolysis tanks M1 and M2 respectively 3 , start stirring, set the substrate concentration to 25% (w / v), put 7.5 tons (1 / 8) of corn stalks with a water...

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Abstract

The invention belongs to the technical field of cellulosic ethanol production. A method for continuous enzymatic hydrolysis of lignocellulose to increase sugar concentration. Multiple enzymatic hydrolysis tanks are started at the same time according to a certain feeding ratio. After the feeding is completed and enzymatic hydrolysis for a specific period of time, the materials are divided, and lignocellulose is added to the primary enzymatic hydrolysis tank. For hydrolytic enzymes, materials and water, only add materials to the secondary enzymolysis tank to increase its solid content to the set value, and maintain high concentration for continuous enzymolysis. The invention transforms the traditional intermittent enzymatic hydrolysis and fermentation into continuous enzymatic hydrolysis, which can give full play to the catalytic activity of the enzyme, improve the substrate conversion rate, and increase the glucose content per unit volume by 10-15%.

Description

technical field [0001] The invention belongs to the technical field of cellulosic ethanol production, and in particular relates to a method for increasing sugar concentration through continuous enzymatic hydrolysis of lignocellulose. Background technique [0002] Lignocellulosic raw materials are the most abundant and cheapest renewable resources on earth. Up to 100 billion tons of lignocellulosic biomass are produced annually through photosynthesis worldwide. my country is a big agricultural country with abundant biomass resources, and the crop straw alone has nearly 8×10 8 tons (about 400 million tons of standard coal), accounting for 10-15% of the country's total primary energy consumption, plus a huge amount of forestry fiber waste and industrial fiber waste residue, the total amount of available lignocellulosic raw materials can reach 2 billion per year more than tons. In lignocellulosic raw materials, cellulose accounts for about 35-45% of the dry weight, and hemice...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10
CPCY02E50/10
Inventor 张晓阳王林风王奇张斐洋米锡耿吴静波
Owner 河南天冠纤维乙醇有限公司
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