A high-efficiency breeding method for two-line rice sterile lines

Abstract

The invention discloses an efficient breeding method of two-line hybrid rice sterile lines. The method of the invention comprises the following steps: 1) selection of combination / variety; 2) construction of the population; 3) preliminary screening; 4) ratoon rice stubbling: selecting proper ratoon rice clipped plant stubble period; 5) ratoon rice seed harvesting: harvesting sterile line seeds per plant during ratoon rice seed maturation, and naturally eliminating seeds which haven't been harvested; 6) single plant optimization and stabilization; 7) primary evaluation of fertility: setting three seeding periods according to the local climate; and detailing heading period and pollen fertility conditions of o sterile lines and contrast in each period; and 8) fertility evaluation: further evaluating and screening the preliminarily screened two-line sterile lines with low starting point conversion temperature, and screening two-line new sterile lines with low starting point conversion temperature. By selecting two-line sterile lines of hybrid progenies, understanding and artificial hybridization processes of resources can be omitted, and breeding efficiency can be greatly raised. The hybrid progeny optimization technology is closer to production actuality, breeding value is higher, and application value is greater.

Description

technical field [0001] The invention belongs to a rice sterile line breeding method, in particular to a two-line rice sterile line high-efficiency breeding method. Background technique [0002] The discovery and breeding utilization of the rice photosensitive GMS line Nongken 58S has created a new era of two-line hybrid rice breeding. Compared with three-line hybrid rice, the two-line hybrid rice has four advantages: ① The photothermo-sensitive genic male sterile gene is a recessive nuclear base, not restricted by the restorer gene, with a wide restoration spectrum and free combination; The fertility gene is a nuclear sterile gene, which is not affected by cytoplasmic genes, and has no negative impact of cytoplasmic effects; ③The photothermosensitive genic male sterile gene is generally a major recessive gene, which is easy to introduce into other genetic backgrounds and is convenient for material improvement; ④Two One line of CMS is dual-purpose, and it is easier to reprod...

AI Technical Summary

Problems solved by technology

[0005] There are also some disadvantages in the use of modern large-scale constant-temperature cold water pools for the identification of sterile lines: ①The cost of large-scale constant-temperature cold-water pools is high, the technology is complicated, and a large amount of capital investment is required; ②The identification of large-scale constant-temperature cold-water pools requires selection of the best detection period and detection of relocated plants , high labor intensity; ③The identification of the two-line CMS belongs to the one-time identification of the CMS strain, and the fertility drift of the fertility cannot be ruled out.

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