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Quick and zero-damage quasipaa spinosa sampling method

A non-damaged, frog-free technology, applied in DNA preparation, recombinant DNA technology, etc., can solve the problems of poor effect of frog DNA, and achieve the effects of saving scientific research funds, less damage to the body, and protecting the environment

Active Publication Date: 2016-05-11
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, using the above method to extract the DNA of spiny-breasted frog is not effective

Method used

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  • Quick and zero-damage quasipaa spinosa sampling method
  • Quick and zero-damage quasipaa spinosa sampling method
  • Quick and zero-damage quasipaa spinosa sampling method

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1, a kind of rapid non-injury sampling method of spinosa spinosa, carries out steps successively:

[0038] 1), use the sterilized plastic film cotton swab to stick (approximately stick repeatedly for 6 seconds) the frog's oral mucus;

[0039] The preparation method of the plastic film cotton swab after above-mentioned sterilization is:

[0040] Take a cotton swab with a cotton head on the top (the length of the cotton swab is about 7.5cm), and the cotton head is made of conventional medical sterile cotton;

[0041] Firstly, the plastic film is sterilized by high-pressure hot and humid steam, that is, the plastic film is sterilized in water vapor with a pressure of 0.1 MPa for 30 minutes; the sterilized plastic film is obtained;

[0042] Then wrap the sterilized plastic film on the cotton head on the cotton swab and carry out high-pressure moist heat steam sterilization, that is, sterilize in water vapor at a pressure of 0.1 MPa for 30 minutes to obtain a ste...

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Abstract

The invention discloses a quick and zero-damage quasipaa spinosa sampling method. According to the method, by means of the oral epidermal cells of quasipaa spinosa and the characteristic of mucus secretion and by adopting homemade sterilization plastic film swabs, intraoral cells of quasipaa spinosa are sampled, oral cells which are taken out are placed in a centrifugal pipe, PBS is added to enable oral cells to be fully blended in PBS, and then sample DNA is extracted by means of a genomic DNA Mini Kit. The method has the advantages that damage to the quasipaa spinosa body is small, the sampling speed is high, the death rate is low, the DNA extraction effect is good, and the method is simple and easy to operate.

Description

technical field [0001] The invention belongs to the research field of frog molecular biology, and in particular relates to a fast and non-damaging sampling method for spinosa spinosa. Background technique [0002] Quasipa aspinosa, also known as stone frog, rock chicken, and stone scale, is a large wild frog unique to my country. It is an important group in the study of the phylogenetic relationship of Ranaidae. It has important scientific research on the evolution and geographical distribution of amphibians. value. Spinosaurus spinosa contains high protein, amino acids, multiple vitamins and minerals. The meat is crisp and tender, and it tastes delicious. Color, treatment of malnutrition and other medicinal effects. [0003] In recent years, as the edible and medicinal value of spiny-breasted frog has been recognized by people, spiny-breasted frog has become more and more popular among consumers, and the market demand has continued to rise. At the same time, the research ...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 郑荣泉孔绅绅胡文芳毛媛媛刘志芳林佳佳蒋泽元
Owner ZHEJIANG NORMAL UNIVERSITY
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