Detection method of pr gene in peripheral blood circulating tumor cells of patients with advanced breast cancer

Abstract

The invention discloses a detection method for PR genes of circulating tumor cells in peripheral blood of a later-period breast cancer patient. The detection method comprises the steps that the CTCs in the peripheral blood of the later-period breast cancer patient are separated and acquired through a film filtering device; the circulating tumor cells in the peripheral blood of the later-period breast cancer patient are identified through a cellular immunity fluorescent technique; thin sections are made through a cell wax block technique; the expression condition of the PR of the circulating tumor cells in the peripheral blood of the later-period breast cancer patient is detected through an immunologic tissue chemical technique. According to the detection method, the enrichment CTCs are separated with the help of an ISET technique, the CTCs are identified by depending on the cellular immunity fluorescent technique, and therefore the difficulties existing in CTC identification only through the ISET technique and the false negativity existing in CTC identification only through an immunological detection technique are overcome. The technology has the advantages that the requirement on equipment is not high, the method is easy to master, and real-time monitoring can be achieved. Through the technical method, the expression condition of the PR of the later-period breast cancer patient can be detected without needing to sample the breast cancer tissue. The technology belongs to a minimally invasive technology or even a non-invasive technology and can achieve real-time detection.

Description

technical field [0001] The invention relates to a new method for detecting PR gene, in particular to a method for detecting PR gene in patients with advanced breast cancer through peripheral blood. Background technique [0002] Currently, the progesterone receptor (PR) as a prognostic factor is a product of ER, which can cause and enhance the response of estrogen to ER, and play a role in promoting and synergizing. The most important indicator is that when only ER is positive, the effective rate of endocrine therapy is 50%-60%, if both ER and PR are positive, the effective rate can be as high as 70%-80%, and the effective rate of those who are negative at the same time is 10%. Existing detection of breast cancer PR needs to obtain tumor tissue samples of primary tumor or metastases through surgery or biopsy. However, not every patient can obtain tumor tissue samples, and it cannot be continuously monitored during treatment detection, which limits its use as an effective eff...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of the traditional circulating tumor cell detection technology and the unsuitable tissue sample collection for patients with advanced breast cancer-thereby inability to evaluate the PR status of patients, the present invention provides a detection method for PR genes in peripheral blood circulating tumor cells of patients with advanced breast cancer: using Separation and acquisition of CTCs in peripheral blood of patients with advanced breast cancer by membrane filtration device; identification of circulating tumor cells in peripheral blood of patients with advanced breast cancer by cell immunofluorescence technology; use of cell wax block technology to make thin-layer sections; and detection of advanced breast cancer by immunohistochemistry PR expression of circulating tumor cells in peripheral blood of patients

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