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Application of specific lectin combination in preparation of test vector for identifying lung cancer type and stage

A lectin, a specific technology, is applied in the field of test vectors for identifying the type and stage of lung cancer, achieving the effect of simple detection operation and controllable cost

Active Publication Date: 2017-07-28
深圳格道糖生物技术有限公司
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Problems solved by technology

Although there is still no complete understanding of the relationship between glycosylation changes and tumor development, these changes are always beneficial to early diagnosis or as an indicator of prognosis

Method used

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  • Application of specific lectin combination in preparation of test vector for identifying lung cancer type and stage
  • Application of specific lectin combination in preparation of test vector for identifying lung cancer type and stage
  • Application of specific lectin combination in preparation of test vector for identifying lung cancer type and stage

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Experimental program
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Embodiment Construction

[0046] The following mainly introduces the key process of the present invention in the research process.

[0047] 1. Experimental part

[0048] 1.1 Reagents and materials

[0049]Glycine, disodium hydrogen phosphate, sodium dihydrogen phosphate, Tween-20 and protease inhibitors were all purchased from Sigma-Aldrich, USA. Bovine serum albumin (BSA) was purchased from Merck, Germany. Cy3 fluorescence was purchased from Amerhsma, USA. Other chemical reagents were of analytical grade without further purification before use. All experimental water was ultrapure water treated by Milli-Q50 pure water system (Millipore, USA). Sephadex G-25 column desalting was purchased from GE Healthcare, USA. Chip hybridization kit was purchased from Bio-Rad, USA. Other common glassware are all made in China. Thirty-seven lectins (see Table 1 for specific names) were purchased from Sigma-Aldrich and Vector, Germany, respectively.

[0050] Table 1 Names of lectins and their specific recogniti...

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Abstract

The present invention clarifies the use of specific lectin combinations in preparing test vectors for identifying lung cancer types in serum samples. Among them, the combination of lectins GSL-I and SNA, the combination of any two or more of the lectins HHL, WFA, WGA, PWM, LEL and MAL-I, the lectins MAL-I, PTL-II, BPL and MAL-II Any two or more combinations correspond to the identification of small cell lung cancer, lung adenocarcinoma, and lung squamous cell carcinoma respectively. The present invention also clarifies the use of additional specific lectin combinations in preparing test vectors for identifying the staging of corresponding types of lung cancer.

Description

technical field [0001] The invention relates to a scheme for using lectins to identify lung cancer types and stages based on serum glycoprotein glycoforms, and specifically relates to the use of specific lectin combinations in preparing test carriers for identifying lung cancer types and stages. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is a malignant tumor originating from the bronchial mucosa or glands. Lung cancer is the most common cause of death from malignant tumors worldwide. Moreover, its incidence is increasing year by year at a rate of about 5%. It is estimated that by 2020, the number of deaths due to lung cancer will reach 50% of the total number of deaths caused by malignant tumors. [0003] Lung cancer has become a major health and social problem that needs to be solved urgently in various countries around the world, especially in developing countries. A nationwide sampling survey conducted in 2010 showed that th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57423G01N33/68G01N2333/4724G01N2440/38
Inventor 李铮贾丽苑马恬然杜昊骐钟耀刚于汉杰梁一倩
Owner 深圳格道糖生物技术有限公司
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