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A method for inducing reproductive diapause of Drosophila spotted wing

A technology of spotted-winged Drosophila and diapause, applied in the field of pest control, can solve problems such as large residues and fruit pollution

Active Publication Date: 2019-03-01
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of Drosophila spotted wing mainly adopts chemical preparations or pesticide preparations to kill them, which have problems such as large residues and easy pollution to fruits.

Method used

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  • A method for inducing reproductive diapause of Drosophila spotted wing
  • A method for inducing reproductive diapause of Drosophila spotted wing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take Drosophila spotted wing eggs in a 2000ml glass jar, and raise them until pupation according to conventional artificial feed and feeding methods; then place the Drosophila spotted wing eggs after pupation in an environment with a temperature of 23-24°C and a humidity of 60%-70%. For light-induced treatment, the light-to-dark ratio of the photoperiod is controlled to be L12:D12, and the light intensity is controlled to be 8000 lx. Select the pupae after the above-mentioned light-induced treatment into a petri dish with a diameter of 10 cm. When a large number of pupae emerge, select female adults within 12 hours of eclosion and carry out light-induced treatment in a 2000ml glass jar. The breeding temperature is controlled at 10 ± 1 ° C. The humidity is 60%-70%, the light-dark ratio of the control photoperiod is L4:D20, and the light intensity is 10000 lx. After 15 days, the reproductive diapause status of Drosophila spotted wing is detected.

Embodiment 2

[0024] Take Drosophila spotted wing eggs in a 2000ml glass jar, and raise them to pupation according to conventional artificial feed and feeding methods; then place the Drosophila spotted wing eggs after pupation in an environment with a temperature of 25-26°C and a humidity of 60%-70%. For light-induced treatment, the light-dark ratio of the photoperiod is controlled to be L20:D4, and the light intensity is controlled to be 12000lx. Select the pupae after the above-mentioned light-induced treatment into a petri dish with a diameter of 10 cm. When a large number of pupae emerge, select female adults within 12 hours of eclosion and carry out light-induced treatment in a 2000ml glass jar. The breeding temperature is controlled at 10 ± 1 ° C. The humidity is 60%-70%, the light-dark ratio of the photoperiod is controlled to be L10:D14, and the light intensity is 10000 lx. After 15 days, the reproductive diapause status of Drosophila spotted wing is detected.

Embodiment 3

[0026] Take Drosophila spotted wing eggs in a 2000ml glass jar, and raise them to pupation according to conventional artificial feed and feeding methods; then place Drosophila spotted wing eggs after pupation in an environment with a temperature of 26-27°C and a humidity of 60%-70%. For light-induced treatment, the light-dark ratio of the photoperiod is controlled to be L14:D10, and the light intensity is controlled to be 10000lx. Select the pupae after the above-mentioned light-induced treatment into a petri dish with a diameter of 10 cm. When a large number of pupae emerge, select female adults within 12 hours of eclosion and carry out light-induced treatment in a 2000ml glass jar. The breeding temperature is controlled at 10 ± 1 ° C. The humidity is 60%-70%, the light-dark ratio of the control photoperiod is L8:D16, and the illuminance is 8000lx. After 15 days, the reproductive diapause status of Drosophila spotted wing is detected.

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Abstract

The invention belongs to the technical field of pest prevention and control, and particularly relates to a method for inducing reproductive diapause of spotted wing drosophila. In the method, spotted wing drosophila after pupation and female adult spotted wing drosophila after eclosion are subjected to lighting induction treatment respectively, so that reproductive diapause of spotted wing drosophila is achieved by effective induction; meanwhile, data shows that during the induction treatment process of spotted wing drosophila after pupation, the light-dark ratio of the photoperiod is controlled to be L(12-20):D(4-12); during the induction treatment process of spotted wing drosophila after eclosion, the light-dark ratio of the photoperiod is controlled to be L(4-10):D(14-20), so that the reproductive diapause rate of spotted wing drosophila can reach 100%.

Description

technical field [0001] The invention belongs to the technical field of pest control, and in particular relates to a method for inducing reproductive diapause of Drosophila spotted wing. Background technique [0002] Spot-winged fruit fly Drosophila suzukii, also known as Cherry Drosophila, Suzuki's Drosophila, belongs to Diptera, Annulus suborder, Drosophilidae, Drosophila genus, and Drosophila subgenus. Drosophila spotted wing is a common pest faced by many orchard crops. Drosophila spotted wing is harmful to the planting of various berries and stone fruits such as blueberries, blackberries, cherries, strawberries, plums, peaches, grapes, figs, kiwis and pears. have greater hazards. [0003] Drosophila spotted wing first appeared in Japan and China, and has recently invaded other countries. Because the ovipositor of Drosophila spotted wing is jagged and has a certain hardness, it can easily pierce the fruit peel and lay eggs inside the intact fruit , the only visible dama...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 翟一凡林清彩郑礼于毅陈浩张思聪李丽莉
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI