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Method for inducing reproductive diapause of spotted wing drosophila

A technology of diapause and diapause of spotted winged fruit flies, which is applied in the field of pest control and can solve problems such as large residues and fruit pollution

Active Publication Date: 2016-06-15
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of Drosophila spotted wing mainly adopts chemical preparations or pesticide preparations to kill them, which have problems such as large residues and easy pollution to fruits.

Method used

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  • Method for inducing reproductive diapause of spotted wing drosophila
  • Method for inducing reproductive diapause of spotted wing drosophila

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take the spot-winged fruit fly eggs in a 2000ml glass jar, and raise them until pupation according to conventional artificial feed and feeding methods; then the spot-winged fruit flies are pupated at a temperature of 23-24℃ and a humidity of 60%-70%. In the light induction treatment, the light-dark ratio of the photoperiod is controlled to be L12:D12, and the light intensity is controlled to 8000lx. The pupae after the above light induction treatment were selected into a petri dish with a diameter of 10 cm. When the pupae erupted in large numbers, the female adults within 12 hours of eclosion were selected for light induction treatment and reared in a 2000 ml glass jar, and the rearing temperature was controlled to 10±1°C. The humidity is 60%-70%, the photoperiod light-dark ratio is controlled to be L4:D20, and the light intensity is 10000lx. After 15 days, the reproductive diapause state of Drosophila melanogaster is detected.

Embodiment 2

[0024] Take the spot-winged fruit fly eggs in a 2000ml glass jar, and feed them until pupation according to conventional artificial feed and feeding methods; then the spot-winged fruit flies are pupated at a temperature of 25-26℃ and a humidity of 60%-70%. In the light induction treatment, the light-dark ratio of the photoperiod is controlled to be L20:D4, and the light intensity is controlled to be 12000lx. The pupae after the above light induction treatment were selected into a petri dish with a diameter of 10 cm. When the pupae erupted in large numbers, the female adults within 12 hours of eclosion were selected for light induction treatment and reared in a 2000 ml glass jar, and the rearing temperature was controlled to 10±1°C. The humidity is 60%-70%, the photoperiod light-dark ratio is controlled to be L10:D14, and the light intensity is 10000lx. After 15 days, the reproductive diapause state of Drosophila melanogaster is detected.

Embodiment 3

[0026] Take the spot-winged fruit fly eggs in a 2000ml glass jar, and raise them until pupation according to conventional artificial feed and feeding methods; then the spot-winged fruit flies will be pupated at a temperature of 26-27℃ and a humidity of 60%-70%. In the light induction treatment, the light-dark ratio of the photoperiod is controlled to be L14:D10, and the light intensity is controlled to 10000lx. The pupae after the above light induction treatment were selected into a petri dish with a diameter of 10 cm. When the pupae erupted in large numbers, the female adults within 12 hours of eclosion were selected for light induction treatment and reared in a 2000 ml glass jar, and the rearing temperature was controlled to 10±1°C. The humidity is 60%-70%, the photoperiod light-dark ratio is controlled to be L8:D16, and the light intensity is 8000lx. After 15 days, the reproductive diapause state of Drosophila melanogaster is detected.

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Abstract

The invention belongs to the technical field of pest prevention and control, and particularly relates to a method for inducing reproductive diapause of spotted wing drosophila. In the method, spotted wing drosophila after pupation and female adult spotted wing drosophila after eclosion are subjected to lighting induction treatment respectively, so that reproductive diapause of spotted wing drosophila is achieved by effective induction; meanwhile, data shows that during the induction treatment process of spotted wing drosophila after pupation, the light-dark ratio of the photoperiod is controlled to be L(12-20):D(4-12); during the induction treatment process of spotted wing drosophila after eclosion, the light-dark ratio of the photoperiod is controlled to be L(4-10):D(14-20), so that the reproductive diapause rate of spotted wing drosophila can reach 100%.

Description

Technical field [0001] The invention belongs to the technical field of pest prevention and control, and specifically relates to a method for inducing reproductive diapause of the spotted fruit fly. Background technique [0002] The spot-winged fruit fly Drosophilasuzukii, also known as the cherry fruit fly, the Suzuki fruit fly, belongs to the Diptera, Cycloschi suborder, Drosophila, Drosophila, and Fruit Drosophila subgenus. The spot-winged fruit fly is a common pest faced by many orchard crops. The spot-winged fruit fly grows a variety of berries and stone fruits such as blueberries, blackberries, cherries, strawberries, plums, peaches, grapes, figs, kiwis and pears. All have greater harm. [0003] The spot-winged fruit fly first appeared in Japan and China, and recently invaded other countries. Because the spot-winged fruit fly's ovipositor is jagged and has a certain degree of hardness, it can easily pierce the fruit skin and lay eggs in the intact fruit. , The only visible d...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 翟一凡林清彩郑礼于毅陈浩张思聪李丽莉
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI