A kind of preparation method and application of Prunella vulgaris polysaccharide extract

A technique for extracting polysaccharides and Prunella vulgaris, applied to medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, to achieve the effects of inhibiting and inducing the proliferation of vascular smooth muscle cells, enriching extraction methods, and saving extraction time

Active Publication Date: 2018-02-27
湖南丛桂草堂医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on its enzyme-assisted ultrasonic hot water extraction of polysaccharides from Prunella vulgaris

Method used

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  • A kind of preparation method and application of Prunella vulgaris polysaccharide extract

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Experimental program
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Effect test

Embodiment 1

[0039] The steps of the preparation method of Prunella vulgaris polysaccharide extract in this embodiment are as follows:

[0040] (1) Pretreatment of medicinal materials:

[0041] The dried ear medicinal material of Prunella vulgaris L. of the Lamiaceae plant was pulverized to 60 meshes, and the ethanol solution with a volume concentration of 95% was added at a liquid-to-material ratio of 20 mL / g. After refluxing for 5 hours at 60°C, the residue was filtered. The filter residue is collected and placed in the air to dry naturally, and then set aside to obtain Prunella vulgaris powder from which lipids, pigments and small molecular sugars have been removed.

[0042] (2) Extraction:

[0043] Take 150g of Prunella vulgaris powder obtained in step (1), add 5% cellulase solution at a material-to-liquid ratio of 45mL / g in an ultrasonic reactor, soak the enzyme for 150min at 60℃, and then under the ultrasonic power of 200W. Perform 2h extraction at ℃, centrifuge the extracted solution at 30...

Embodiment 2

[0056] The steps of the preparation method of Prunella vulgaris polysaccharide extract in this embodiment are as follows:

[0057] (1) Pretreatment of medicinal materials:

[0058] The dried ear medicinal material of Prunella vulgaris L. of the Lamiaceae plant was pulverized to 60 meshes, and the ethanol solution with a volume concentration of 95% was added at a liquid-to-material ratio of 15 mL / g. After refluxing for 4.5 hours at 75°C, the residue was filtered , Collect the filter residue and place it in the air to dry naturally, and then set aside to obtain Prunella vulgaris powder from which fats, pigments and small molecular sugars have been removed.

[0059] (2) Extraction:

[0060] Take 150g of the Prunella vulgaris powder obtained in step (1), add 4.5% cellulase solution at a material-to-liquid ratio of 50mL / g in an ultrasonic reactor, soak the enzyme for 140min at 65℃, and then under the ultrasonic power of 400W 60 Extract for 2.5 hours at ℃, centrifuge the extracted solution...

Embodiment 3

[0073] The steps of the preparation method of Prunella vulgaris polysaccharide extract in this embodiment are as follows:

[0074] (1) Pretreatment of medicinal materials:

[0075] The dried ear medicinal material of Prunella vulgaris L., a Lamiaceae plant, was crushed to 80 meshes, and a 90% volume concentration ethanol solution was added at a material-to-liquid ratio of 10 mL / g. After refluxing at 55°C for 5 hours, the residue was filtered. The filter residue is collected and placed in the air to dry naturally, and then set aside to obtain Prunella vulgaris powder from which lipids, pigments and small molecular sugars have been removed.

[0076] (2) Extraction:

[0077] Take 150g of Prunella vulgaris powder obtained in step (1), add 5.5% cellulase solution at a material-to-liquid ratio of 30mL / g in an ultrasonic reactor, soak the enzyme hydrolysis at 55℃ for 160min, and then under the ultrasonic power of 300W 70 Perform extraction at ℃ for 3 hours, centrifuge the extracted solution...

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Abstract

The invention discloses a preparation method of spica prunellae polysaccharide extracts.The method comprises the following steps that spica prunellae is smashed and added into an ethanol water solution on the basis that the ratio of solid to liquid is 10-20 mL / g, reflux extraction is performed, and filtering and drying are performed to obtain spica prunellae powder; the spica prunellae powder is added into a cellulase water solution on the basis that the ratio of solid to liquid is 30-50 mL / g, soaking and enzymolysis are performed, ultrasonic extraction is performed, and a supernate is collected; concentration is performed; absolute ethyl alcohol with the volume being 3-4 times that of the obtained concentrated product is added into the obtained concentrated product, alcohol precipitation is performed, and precipitations are collected; the obtained precipitations are dissolved into a small amount of water and added into a n-butyl alcohol-chloroform solution with the volume being one quarter that of the precipitations on the basis of the volume ratio being 4:1, stirring, standing and configuring are performed, and a supernate is collected to obtain a deproteinization polysaccharide solution; secondary alcohol precipitation is performed, and precipitations are collected to obtain deproteinization polysaccharide; the obtained deproteinization is freezen and dried to obtain dried spica prunellae polysaccharide extract powder.The method is high in spica prunellae polysaccharide extraction rate, simple in technology, green, free of toxicity, environmentally friendly and suitable for industrial popularization.

Description

Technical field [0001] The invention relates to the technical field of traditional Chinese medicine, and more specifically to a preparation method and application of a Prunella vulgaris polysaccharide extract. Background technique [0002] Prunella vulgaris is the dry ear of Prunella vulgaris (Prunella vulgaris L.). In my country, Prunella vulgaris is concentrated in the Huai River Basin and the middle and lower reaches of the Yangtze River. Jiangsu, Anhui, Zhejiang and Henan have the most abundant wild resources. ; In addition, South Korea, Japan, Ukraine and Kashmir are also distributed. The main components of Prunella vulgaris include triterpenes and their saponins, sterols, flavonoids, coumarins, polysaccharides and so on. Prunella vulgaris has a long history of medicinal use. Its main effects include clearing liver and purging fire, improving eyesight, dispelling swelling and swelling; it is used for red and swollen eyes, night pain, headache, dizziness, scrofula, gallbladde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P9/14
CPCA61K31/715A61K36/536C08B37/0003
Inventor 林丽美夏伯候廖端芳周亚敏
Owner 湖南丛桂草堂医药有限公司
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