The invention relates to an extracting and purifying method for
prunella vulgaris total flavonoids. The method comprises the steps: (1) fetching and crushing a
prunella vulgaris medicinal material, sieving the crushed
prunella vulgaris medicinal material, adding 5-95%
ethanol, of which the weight is 1-30 times that of the crushed
prunella vulgaris medicinal material, into the crushed
prunella vulgaris medicinal material, carrying out refluxing extraction for 1-3 times, carrying out refluxing extraction for 1-5 hours at a time, filtrating extractions, and combining filtrates, so as to obtain an extract; (2) heating to concentrate the extract until the concentration of the extract is 0.05-0.5g / mL, and then, adjusting the pH value to 1-7, so as to obtain a liquid sample; (3) loading the liquid sample to macroporous adsorption resin NKA-9, carrying out
impurity removal with 1-10BV water, and then, eluting with 1-10BV 10-95%
ethanol; (4) continuously carrying out a purifying process in the step (3) repeatedly so as to obtain enrichment,
drying the enrichment at reduced pressure until the enrichment becomes
powder or extractum, thereby obtaining the
prunella vulgaris total flavonoids. The invention also provides application of the prunella vulgaris total flavonoids in preparation of drugs for treating
liver cancer. Proven by pharmacological
drug effect researches, the prunella vulgaris total flavonoids have obvious
liver tumor resisting activity and are of an obvious
dose-effect relationship, so that a theoretical and experimental basis is provided for the development of new
liver tumor resisting drugs, which are prepared from enrichment of the prunella vulgaris total flavonoids and have extensive clinical use value and commercial development value, and industrial production.