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Prunella vulgaris polysaccharide having immune activity, preparation method and application thereof

A composition and technology of Prunella vulgaris, applied in the field of Prunella vulgaris polysaccharide composition and preparation thereof, can solve the research and patent report on the immune activity of the Prunella vulgaris polysaccharide composition, and the research report on the Prunella vulva polysaccharide composition, etc. question

Inactive Publication Date: 2007-05-23
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After investigation, there is no research report on the composition of Prunella vulgaris polysaccharides with a molecular weight of more than 5000 Da, and there are no researches and patent reports on the immune activity of Prunella vulgaris polysaccharide compositions

Method used

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  • Prunella vulgaris polysaccharide having immune activity, preparation method and application thereof
  • Prunella vulgaris polysaccharide having immune activity, preparation method and application thereof
  • Prunella vulgaris polysaccharide having immune activity, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Preparation of Prunella vulgaris polysaccharide composition

[0039] 5.0kg Prunella vulgaris, decoct twice, add 20 times of water each time, 1 hour each time, filter the extract, concentrate the filtrate under reduced pressure at 40-60°C to about 5000ml, add a precipitant (ethanol) until the content reaches 70 %, let stand overnight. The supernatant was discarded, 3000-5000 rpm, refrigerated and centrifuged for 15 minutes to separate the precipitate, and the precipitate was vacuum-dried at 40-50°C to obtain about 300 g of crude polysaccharide.

[0040] Take 1500ml of water, add 100g of crude polysaccharide, decoct and stir to fully dissolve, cool down, use cellophane (cut-off value about 3000-5000Da) running water dialysis for 48 hours, dialyze the internal fluid at 3000-5000 rpm, centrifuge at 40°C and rotate the supernatant Concentrate by evaporation to about 1600ml, refrigerate and centrifuge (10000-20000 rpm) for 10-20 minutes, and freeze-dry to obtain...

Embodiment 2

[0041] Example 2 Separation and purification of Prunella vulgaris polysaccharide composition

[0042]Put the aqueous solution of the preliminary purified Prunella vulgaris polysaccharide (volume about 200ml, solid content about 5.0g) on ​​the DEAE-sepharose column, sequentially eluted with 0-1.0mol / L Nacl solution stepwise, collected in separate tubes, and the collected solution was washed with sulfuric acid -Detection of polysaccharides by phenol method.

[0043] The sugar reaction positive collections eluted with various solvents were combined separately, concentrated by rotary evaporation at 40°C, dialyzed with distilled water for 48 hours with a dialysis bag (cut-off value 3000-5000Da), and the dialyzed internal solution was concentrated by rotary evaporation at 40°C to less than 50ml, and then freeze-dried , get four polysaccharide parts A, B, C, D respectively. The molecular weight and purity of these four polysaccharide parts were measured respectively. The chromatogra...

Embodiment 3

[0045] Example 3 Purity Identification of Prunella vulgaris Polysaccharide Composition

[0046] The purity of the isolated and purified polysaccharide was determined by HPSEC. The specific test conditions and methods are as follows: Waters high-performance liquid phase system, Ultrahydroge tandem column (the effective test molecular weight range is 2,000 to 2 million), Waters 2410 differential detector, Waters 2487 dual-wavelength ultraviolet detector (215 and 280nm), Evaporative light dispersion detector, online degassing device, Millennium32GPC data processing software, mobile phase is 0.003mol / L NaAc, flow rate is 0.5ml / min. Get a little (about 2 mg) of the test sample and dissolve it with mobile phase, prepare about 1% sample solution, centrifuge at 10000 rpm for ten minutes, manually inject 20 ml, and judge the purity of the polysaccharide component according to the chromatogram. The results showed that components A, B-1 and B-2 in the composition were all polysaccharide...

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Abstract

The invention relates to a prunella spike polysaccharide composite with immunity activity, and relative production, wherein its production comprises that: boiling the dry fruit of Prunella vulgaris L, extracting, depositing with alcohol, filtering the components whose molecule weight is higher than 3000Da, filtering and purifying. The polysaccharide content is higher than 50%, the polysaccharide molecule is higher than 5000Da. And it is formed by A, B-1, B-2, C, and D. The invention can improve lymphocyte transfer gain and induce IFN- gamma, to be used as active component to prepare the drug that improves the immunity of mammal.

Description

technical field [0001] The invention relates to active ingredients of traditional Chinese medicine and an extraction and separation method, in particular to an immunoactive Prunella vulgaris polysaccharide composition and its preparation method and application. Background technique [0002] Prunella is a plant of the genus Prunella L., and there are about 15 species in the world, which are similar to each other. They are widely distributed in Eurasian temperate regions and tropical mountainous areas, and are also distributed in northern Africa and North America. There are 4 species and 3 varieties produced in China, one of which is introduced. Mainly produced in Hunan, Anhui, Zhejiang, Jiangsu. The Prunella vulgaris recorded in the Chinese Pharmacopoeia is the dried fruit ear of Prunella vulgaris L., a plant of the Labiatae family. It has the effects of clearing fire, improving eyesight, dispelling stagnation, and reducing swelling. Prunella vulgaris mainly contains triterp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/536A61K31/715A61P37/04
Inventor 冯怡徐德生徐宏喜
Owner SHANGHAI UNIV OF T C M
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