Extracting and purifying method for prunella vulgaris total flavonoids and application of prunella vulgaris total flavonoids

Abstract

The invention relates to an extracting and purifying method for prunella vulgaris total flavonoids. The method comprises the steps: (1) fetching and crushing a prunella vulgaris medicinal material, sieving the crushed prunella vulgaris medicinal material, adding 5-95% ethanol, of which the weight is 1-30 times that of the crushed prunella vulgaris medicinal material, into the crushed prunella vulgaris medicinal material, carrying out refluxing extraction for 1-3 times, carrying out refluxing extraction for 1-5 hours at a time, filtrating extractions, and combining filtrates, so as to obtain an extract; (2) heating to concentrate the extract until the concentration of the extract is 0.05-0.5g / mL, and then, adjusting the pH value to 1-7, so as to obtain a liquid sample; (3) loading the liquid sample to macroporous adsorption resin NKA-9, carrying out impurity removal with 1-10BV water, and then, eluting with 1-10BV 10-95% ethanol; (4) continuously carrying out a purifying process in the step (3) repeatedly so as to obtain enrichment, drying the enrichment at reduced pressure until the enrichment becomes powder or extractum, thereby obtaining the prunella vulgaris total flavonoids. The invention also provides application of the prunella vulgaris total flavonoids in preparation of drugs for treating liver cancer. Proven by pharmacological drug effect researches, the prunella vulgaris total flavonoids have obvious liver tumor resisting activity and are of an obvious dose-effect relationship, so that a theoretical and experimental basis is provided for the development of new liver tumor resisting drugs, which are prepared from enrichment of the prunella vulgaris total flavonoids and have extensive clinical use value and commercial development value, and industrial production.

Description

technical field

[0001] The invention relates to the technical fields of chemistry and medicine, in particular to a method for extracting and purifying total flavonoids from Prunella vulgaris and its application. Background technique

[0002] Cancer is a general term for a large class of malignant tumors. It is the world's largest disease that seriously threatens human health. Various inducing factors such as environmental pollution, chemical pollution (chemical toxins), ionizing radiation, free radical toxins, etc. can lead to cancer happened. Current cancer treatments include surgery, chemotherapy, radiotherapy, traditional Chinese medicine, etc. These treatments have certain curative effects on cancer, but their effects are limited, and radiotherapy and chemotherapy can also cause great damage to normal tissues. Therefore, The treatment of cancer is still a great test facing mankind. TCM treatment of cancer has now been widely recognized by medical experts at home and ab...