Genotyping diagnostic kit for multiple granulomatosis vasculitis and arteritis arteriosis and method of use

Abstract

The invention belongs to the field of genotyping diagnosis, and in particular relates to a genotyping diagnostic kit for multiple granulomatous vasculitis and microarteritis and a method of use thereof, including SNP detection probes for granulomatous polyangiitis gene and microarteritis gene, Multiple PCR primers, multiple PCR reaction solution, hybridization solution and eluent. Compared with the prior art, the present invention has the following beneficial effects: by detecting the specific genetic markers of the 5 SNP sites related to GPA and MPA and the changes in the expression level of disease susceptibility genes in blood cells, the present invention can treat such diseases Molecular genetic typing was performed to distinguish between GPA and MPA. And it can assess the risk of AAV in normal individuals carrying susceptibility genes, and has good molecular typing efficiency.

Description

technical field [0001] The invention belongs to the field of genotyping diagnosis, and in particular relates to a genotyping diagnosis kit for multiple granulomatous vasculitis and microarteritis and a use method thereof. Background technique [0002] Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (ANCA Associated Vasculitis, AAV) includes granulomatosis with polyangiitis (GPA) and microscopic arteritis (Microscopic Polyangiitis, MPA). According to reports, 1,353 cases of ANCA-associated small vessel vasculitis have been newly detected in the clinical examination room of Peking University Institute of Nephrology in the past five years, indicating that this type of disease is relatively common in my country. At present, ANCA is the main serological diagnostic index of AAV disease. So far, there is no molecular genetic diagnosis and distinction between GPA and MPA. [0003] AAV diseases include two types, GPA and MPA. The current diagnosis of such diseases ...

AI Technical Summary

Problems solved by technology

These data indicate that the specificity of ANCA serological examination is low, and it cannot accurately distinguish between GPA and MPA disease types; moreover, its sensitivity and detection rate are greatly affected by the active state of the disease, and the positive rate of patients in the active stage of the disease is high. Positive rate of inactive patients is low

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