Gene typing diagnosis kit for multiple granulomatous angiitis and micro-arteritis and use method

Abstract

The invention belongs to the field of gene typing diagnosis, and particularly relates to a gene typing diagnosis kit for multiple granulomatous angiitis and micro-arteritis and a use method. The gene typing diagnosis kit comprises an SNP detection probe for multiple granulomatous angiitis genes and micro-arteritis genes, multiple PCR primers, multiple PCR reaction liquid, hybridization liquid and eluent. Compared with the prior art, the gene typing diagnosis kit has the following beneficial effects: by detecting the change of the expression level of specific genetic markers and disease-predisposing genes of GPA and MPA related 5 SNP sites in blood cells, the diseases are subjected to molecular genetics typing so as to distinguish GPA and MPA diseases; and the attack risk of the normal individual AAV carrying predisposing genes is evaluated, and good molecular typing efficiency is achieved.

Description

technical field [0001] The invention belongs to the field of genotyping diagnosis, and in particular relates to a genotyping diagnosis kit for multiple granulomatous vasculitis and microarteritis and a use method thereof. Background technique [0002] Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (ANCA Associated Vasculitis, AAV) includes granulomatosis with polyangiitis (GPA) and microscopic arteritis (Microscopic Polyangiitis, MPA). According to reports, 1,353 cases of ANCA-associated small vessel vasculitis have been newly detected in the clinical examination room of Peking University Institute of Nephrology in the past five years, indicating that this type of disease is relatively common in my country. At present, ANCA is the main serological diagnostic index of AAV disease. So far, there is no molecular genetic diagnosis and distinction between GPA and MPA. [0003] AAV diseases include two types, GPA and MPA. The current diagnosis of such diseases ...

AI Technical Summary

Problems solved by technology

These data indicate that the specificity of ANCA serological examination is low, and it cannot accurately distinguish between GPA and MPA disease types; moreover, its sensitivity and detection rate are greatly affected by the active state of the disease, and the positive rate of patients in the active stage of the disease is high. Positive rate of inactive patients is low

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