A method for rapidly counting bacterial colonies

A fast, colony-based technology, applied in the field of microbiology, can solve the problems of high cost, general promotion constraints, and lack of counting result inspection, etc., to achieve the effect of facilitating popularization and application and ensuring counting accuracy

Active Publication Date: 2018-09-28
INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above methods all require specific instruments and equipment to ensure the quality of image acquisition or image processing, and the cost is high, resulting in the restriction of generalization; and the above methods lack the inspection of counting results

Method used

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  • A method for rapidly counting bacterial colonies
  • A method for rapidly counting bacterial colonies
  • A method for rapidly counting bacterial colonies

Examples

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Embodiment Construction

[0051] Such as figure 1 A kind of colony counting method shown is characterized in that, described method comprises the following steps:

[0052] Step 1: Use a digital camera to collect images of microbial colonies on the culture medium in the culture dish. The specific requirements are: the shooting angle is directly above the culture medium, and the background should be as uniform in color as possible, preferably in a color that has a large contrast with the color of the colonies. The resolution of the camera should not be less than 300,000 pixels (640*480). The higher the pixel, the better the effect. When shooting, while ensuring that the entire culture dish is captured, try to ensure that the culture medium is covered with the shooting range.

[0053] Step 2: Take the lower left corner of the image taken by the petri dish as the origin, establish a plane Cartesian coordinate system according to the actual size of the petri dish, and define the original image coordinates, ...

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Abstract

The invention relates to a bacterial colony quick counting method. The method includes the following steps that: a digital camera is utilized to carry out image acquisition on a microbial colony on a culture medium in a culture dish; a plane Cartesian coordinate system is established, an image I is generated; graying processing is performed on the image I, so that an image II can be generated; binarization processing is performed on the image II, so that an image III can be generated; the image III is cut, so that an image IV is generated; the image IV is vectorized, so that a vectorized image (1) is obtained; the vectorized image (1) is analyzed, and the number of bacterial spots forming bacterial blocks is calculated; the center of gravity (center) and radius of each bacterial spot is calculated; the vector points of the centers of gravity (centers) of the bacterial spots are simulated, so that a vector image (2) can be generated; circular vector graph spots simulating the bacterial spots and a planar vector graph-based vector image (3) are generated according to the centers and radiuses of the bacterial spots; and the area of each graph spot in the vector image (3) is calculated, and the number of the bacterial spots is calculated. The bacterial colony quick counting method of the invention has low requirements for image quality and can be realized universally. With the method adopted, counting can be carried out quickly and accurately, and the growth area of the bacterial colony can be calculated.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and in particular relates to a colony counting detection method. Background technique [0002] Colony counting is one of the most common experiments in microbial experiments. It is an effective method for counting the number of bacteria contained in items. It is widely used in food, beverages, medicines, biological products, cosmetics, sanitary products, drinking water, domestic sewage, industrial wastewater, clinical Examination of the number of bacteria in the specimen. Generally, a petri dish is used to culture on a solid medium (inside) under specific conditions, and a group of daughter cells that are visible to the naked eye with a certain shape, structure, and other characteristics centered on the microbial mother cell, that is, a colony, is cultivated. The presence and quantity of pathogenic bacteria can be determined by colony counting, or it can be used to determine the degree of c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06M11/00
CPCG06M11/00
Inventor 苑学霞张勇王文博李瑞菊
Owner INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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