A method for rapidly counting bacterial colonies

Abstract

The invention relates to a bacterial colony quick counting method. The method includes the following steps that: a digital camera is utilized to carry out image acquisition on a microbial colony on a culture medium in a culture dish; a plane Cartesian coordinate system is established, an image I is generated; graying processing is performed on the image I, so that an image II can be generated; binarization processing is performed on the image II, so that an image III can be generated; the image III is cut, so that an image IV is generated; the image IV is vectorized, so that a vectorized image (1) is obtained; the vectorized image (1) is analyzed, and the number of bacterial spots forming bacterial blocks is calculated; the center of gravity (center) and radius of each bacterial spot is calculated; the vector points of the centers of gravity (centers) of the bacterial spots are simulated, so that a vector image (2) can be generated; circular vector graph spots simulating the bacterial spots and a planar vector graph-based vector image (3) are generated according to the centers and radiuses of the bacterial spots; and the area of each graph spot in the vector image (3) is calculated, and the number of the bacterial spots is calculated. The bacterial colony quick counting method of the invention has low requirements for image quality and can be realized universally. With the method adopted, counting can be carried out quickly and accurately, and the growth area of the bacterial colony can be calculated.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and in particular relates to a colony counting detection method. Background technique [0002] Colony counting is one of the most common experiments in microbial experiments. It is an effective method for counting the number of bacteria contained in items. It is widely used in food, beverages, medicines, biological products, cosmetics, sanitary products, drinking water, domestic sewage, industrial wastewater, clinical Examination of the number of bacteria in the specimen. Generally, a petri dish is used to culture on a solid medium (inside) under specific conditions, and a group of daughter cells that are visible to the naked eye with a certain shape, structure, and other characteristics centered on the microbial mother cell, that is, a colony, is cultivated. The presence and quantity of pathogenic bacteria can be determined by colony counting, or it can be used to determine the degree of c...

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Problems solved by technology

[0005] The above methods all require specific instruments and equipment to ensure the quality of image acquisition or image processing, and the cost is high, resulting in the restriction of generalization; and the above methods lack the inspection of counting results

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