A single neuron culture mold for studying pressure gradient damage and its preparation method and application

A pressure gradient, single neuron technology, applied in biochemical equipment and methods, culture process, tissue culture, etc.

Active Publication Date: 2018-04-24
李昱辉 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are deficiencies in the study of neuron stress

Method used

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  • A single neuron culture mold for studying pressure gradient damage and its preparation method and application
  • A single neuron culture mold for studying pressure gradient damage and its preparation method and application
  • A single neuron culture mold for studying pressure gradient damage and its preparation method and application

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Embodiment Construction

[0040] This embodiment provides a single neuron culture mold for studying pressure gradient damage, the preparation method of which is as follows:

[0041] 1) Preparation of photomask

[0042] Axon outgrowth photomask 1-1 and cell trapping spot photomask 1-2 were prepared as figure 1 , figure 2 As shown, the light-transmitting part of the axon growth photomask 1-1 is rectangular, with a length and width of 1 cm and 20 μm, respectively; the light-transmitting part of the cell capture spot photomask 1-2 is circular, with a diameter of 150 μm; The photomask is a film material;

[0043] The photomask is prepared by Shenzhen Guangyi Co., Ltd.;

[0044] 2) Preparation of hydrogel solution

[0045] Dissolve GelMA into phosphate buffered saline (DPBS), prepare a 5% final concentration of GelMA hydrogel solution, and add 0.5% photosensitizer to obtain a hydrogel solution;

[0046] The photosensitizer is Irgacure 2959;

[0047] 3) Preparation of trapezoidal GelMA hydrogel

[004...

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Abstract

The invention provides a single neuron culture mould for researching pressure gradient damage, and a preparation method and application thereof. The mould comprises a glass piece, a trapezoidal hydrogel layer and a neuronal cell-containing hydrogel layer; the trapezoidal hydrogel layer is sized as a trapezoid through a trapezoid groove; the neuronal cell-containing hydrogel layer is positioned at the head end (H) of the trapezoidal hydrogel layer; the trapezoidal hydrogel layer and the neuronal cell-containing hydrogel layer are solidified on the glass piece. Trapezoidal axon growth hydrogel with a high head and a low tail is prepared by performing light polymerization twice, and neuronal cells are fixed to the head end (H) of the axon growth hydrogel, external force with fixed intensity is loaded in a direction which is perpendicular to the axon growth hydrogel, and the pressure gradient felt by a neuron long axis is simulated by utilizing the gradient stress formed by different deformation degrees after gel is stressed.

Description

technical field [0001] The invention belongs to the field of cell damage research, in particular to a single neuron culture mold for studying pressure gradient damage, a preparation method and application thereof. Background technique [0002] Cells in the body experience various stresses (shearing force, pressure, stretching force, etc.) depending on their location. When cells are stressed beyond the tolerance range, damage will occur. It is an important topic in the field of biomedicine to study the impact of this damage process on cell behavior. In order to study the damage of force to cells, various mechanical loading systems have been developed. Among them, the flexcell system in the United States can complete the loading of cell fluid shear force, pressure, tension, etc. Most of these loading systems are mechanically loaded on the whole cell, that is, a single cell receives the same force at the same time, which can satisfy most researches. But there are deficiencies...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0793C12N5/079C08F289/00C08F220/10
CPCC08F289/00C12N5/0619C12N5/0622C12N2527/00C12N2533/30C12N2533/54C08F220/10
Inventor 武珅王宁利张敬学毛迎燕李昱辉徐峰
Owner 李昱辉
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