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A kind of goat straw frozen fine production method
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A production method and thin tube technology, applied in the preservation, application, and animal husbandry of human or animal bodies, to achieve the effect of breaking through the limit of animal life, promoting application and promotion, and achieving long-term preservation
Active Publication Date: 2021-05-04
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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However, the production of thin tube frozen semen is still a complicated process, which requires not only suitable diluent, protective agent, equilibrium temperature and equilibrium time, cooling rate and thawing rate, but also equipment such as programmed cooling device, filling and sealing
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Embodiment 1
[0018] The invention relates to a production method of goat straw frozen semen, which comprises the preparation of frozen diluent, semen collection and microscopic examination, semen dilution, semen subpackage and balance, semen freezing, thawing and re-inspection. The specific operation steps are as follows:
[0019] (1) Preparation of frozen diluent: under aseptic conditions, aseptically dispense the purchased Casu OVIXCELL Sheep Semen Diluent (Product No.: 020997) into 50ml centrifuge tubes and pre-warm them in a 34°C water bath;
[0020] (2) Semen collection and microscopic examination: The semen of 5 Hainan black goat rams was collected by electric stimulation method, and the semen quality was routinely inspected, and the selected vigor was not less than 65%, the live rate was not less than 60%, and the density was not low. at 10 8 pcs / ml of fresh essence is used for the subsequent production of frozen essence;
[0021] (3) Dilution: Slowly pour the pre-warmed frozen di...
Embodiment 2
[0027] The invention relates to a production method of goat straw frozen semen, which comprises the preparation of frozen diluent, semen collection and microscopic examination, semen dilution, semen subpackage and balance, semen freezing, thawing and re-inspection. The specific operation steps are as follows:
[0028] (1) Preparation of frozen diluent: Under sterile conditions, prepare frozen diluent according to the following method, accurately weigh 3.07g of Tris, 1.26g of fructose, 1.64g of citric acid, 100000IU of penicillin, 0.1g of streptomycin, and 5mL of glycerin , 1.6g of phosphatidylcholine and 0.4g of cholesterol were added to 85ml of double distilled water to adjust the pH to 6.8-7.0, added to double distilled water to make up to 100mL, filtered through a sterile filter with a pore size of 0.22μm, and dispensed to 50ml under sterile conditions Centrifuge the tube and pre-warm it in a 34°C water bath;
[0029] (2) Semen collection and microscopic examination: The s...
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Abstract
The invention discloses a production method of goat thin tube frozen semen, belongs to the technical field of livestock and poultry frozen semen production, and in particular relates to a production method of goat thin tube frozen semen. The specific steps of the goat thin tube frozen semen production method are as follows: 1. Preparation of frozen diluent; 2. Semen collection and microscopic examination; 3. Dilution; 4. Subpackaging; 5. Balance; 6. Freezing; 7. Thawing and recheck. The method for producing frozen semen with thin tubes of the present invention is simple and quick to operate, and does not require complex instruments and equipment. When the quality of fresh semen meets the requirements, the survival rate of frozen semen with thin tubes after freezing and thawing is not lower than 0.35, which can not only meet the requirements of artificial insemination for frozen semen The requirement of sperm live rate can also fully improve the utilization rate of rams, break through the limitation of animal life, realize the long-term preservation of ram semen, and greatly promote the application and promotion of goat frozen semen.
Description
technical field [0001] The invention belongs to the technical field of production of frozen semen of livestock and poultry, and in particular relates to a production method of goat thin tube frozen semen. Background technique [0002] Goat semen cryopreservation is a major breakthrough in artificial insemination technology. It not only expands the use efficiency of ram semen in terms of time and space, and gives full play to the reproductive performance of high-quality rams, but also protects high-quality or special breeding livestock and poultry. It is also of great significance in terms of planting and introduction, and reducing production costs. my country's research on goat semen cryopreservation technology began in the 1980s. After years of experimental research and production practice exploration, key technological breakthroughs have been made in the production of goat granule frozen semen and thin tube frozen semen, and some areas have established Procedures for the p...
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Application Information
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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 施力光李义书周汉林侯冠彧
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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