Molecular tagging primer and method for distinguishing South China Sea coryphaena equiselis and coryphaena hippurus

A technology of molecular markers and marker primers, which is applied in the field of molecular biology, can solve the problems of mixed germplasm of mahi-mahi fish, and achieve the effects of strong repeatability, simple identification method and stable results

Inactive Publication Date: 2018-04-03
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a molecular marker primer and method for distinguishing South China Sea spiny mahi and mahi mahi, which helps to solve the problem of mixed germplasm of fish of the genus Mahi mahi. The method of the present invention is simple to operate, easy to master, and accurate high sex

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  • Molecular tagging primer and method for distinguishing South China Sea coryphaena equiselis and coryphaena hippurus

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Embodiment Construction

[0017] The technical solution of the present invention will be further explained by examples below, but the protection scope of the present invention is not limited by any form of examples.

[0018] A molecular marker primer and method for distinguishing South China sea mahi-mahi and mahi-mahi, comprising the following steps:

[0019] (1) Select the mahi-mahi and mahi-mahi sample group collected from the survey of swimming animals in the West Pacific and the South China Sea to form the mahi-mahi sample group and the mahi-mahi sample group, and extract the total genomic DNA of the two sample groups;

[0020] (2) Carrying out PCR amplification reaction with the extracted total genomic DNA as template;

[0021] (3) Purify and sequence the successfully amplified PCR products, and compare the sequencing results.

[0022] The obtained molecular marker primer sequences were MiFish-U-F: 5'-GTCGGTAAAACTCGTGCCAGC-3'; MiFish-U-R: 5'-CATAGTGGGGTATCTAATCCCAGTTTG-3'.

[0023] Take 1ul of ...

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Abstract

The invention relates to a molecular tagging primer for distinguishing South China Sea coryphaena equiselis and coryphaena hippurus. The sequence of the primer is MiFish-U-F: 5' -GTCGGTAAAACTCGTGCCAGC-3'; Mifish-U-R: 5'-CATAGTGGGGTATCTAATCCCAGTTTG-3. The molecular tagging primer helps to solve the problem that coryphaena fish is confused in germplasm, and the method provided by the invention is simple to operate, easy to master and high in accuracy.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a molecular marker primer and a method for identifying South China Sea spiny mahi-mahi and mahi-mahi. Background technique [0002] Dolphins belong to the class Actinoptera, Perciformes, Mahidae, and the genus Dolphin. Dolphins are rare in China, and occasionally appear in the southwestern waters of Taiwan. Its external morphological characteristics are very similar to those of dolphin mahi, the main features are: body extremely elongated and flattened laterally, head tall, tapering to the rear, the highest point is on the head; forehead with a bony protuberance, long and blunt snout, eyes Large, large mouth, slightly inclined, lower jaw slightly protruding upper jaw, upper and lower jaws, palatine bone and tongue surface all have teeth, upper and lower jaw teeth are multi-row, and the shape of teeth is sparsely arranged; the body is not easy to fall off small round scales, the h...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李渊张静宋普庆林龙山
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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