A nitrogen-fixing bacterium pj3-5 capable of degrading petroleum and tolerant to petroleum and its application

A nitrogen-fixing bacteria and oil technology, applied in the field of microorganisms, can solve the problems of excessive oxygen consumption, aerobic biological oil degradation limitation, failure, etc., and achieve the effect of positive application prospects.

Active Publication Date: 2021-06-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of nutrients can lead to some potential problems, such as excessive consumption of oxygen in the environment, forming an anaerobic environment, which in turn reduces the ability of indigenous microorganisms to degrade oil
Aerobic biodegradation of petroleum is often limited by combined nitrogen levels
In 2011, Mercer summarized the reported effects of biological treatment (bio-stimulation and degradative agent addition) in beach pollution treatment, and found that 10 reports were successful and 6 reports failed

Method used

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  • A nitrogen-fixing bacterium pj3-5 capable of degrading petroleum and tolerant to petroleum and its application
  • A nitrogen-fixing bacterium pj3-5 capable of degrading petroleum and tolerant to petroleum and its application
  • A nitrogen-fixing bacterium pj3-5 capable of degrading petroleum and tolerant to petroleum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Nitroase activity analysis of the strain PJ3-5 of the present invention

[0042] 1, nitrogenase assay conditions:

[0043] 1) The strain PJ3-5 (referred to as PJ3-5 strain) of the present invention activates twice at 30 ° C in the LB medium, and after the transfer liquid culture 10HR, K.oxytoca (no nitrogen-free) medium wash bacteria, adjusted Start OD 600 = 0.2 or so, inoculated to 5 mlk.oxytoca (no nitrogen-free) medium, anaerobutant tube, 30 ° C, and the rotational speed of 200 RMP.

[0044] 2) When cultured 4Hr, 8Hr, 12Hr, 16Hr, 20Hr, 24HR, after the anaerobic tube is replaced into argon, after adjusting different oxygen concentrations, the acetylene is injected, and the reaction is injected into trichloroacetic acid after 0.5 h, then take the reaction, then take 100 μL of gas was measured in the gas in the gas in the gas spectroscopy (SQ-204 gas chromatography, FID detector, 2 meter filling column, and internal GDX502).

[0045] SQ-204 gas chromatography test...

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Abstract

The invention relates to the field of microorganisms, and specifically discloses a nitrogen-fixing bacterium PJ 3-5 that can degrade petroleum and tolerate petroleum, and its preservation number is: CGMCC No.14658. The nitrogenase activity analysis shows that the bacterial strain of the present invention has strong nitrogenase activity, and can still maintain 75% nitrogenase activity under the condition of 5% oxygen concentration. The strain can still maintain its nitrogenase activity in a petroleum-polluted environment with a petroleum concentration of 1%-5%. Not only that, in the nitrogen-free medium of 1% petroleum, the presence of the strain of the present invention can also improve the growth ability of other petroleum-degrading bacterial strains by 60%. Not only that, the strain of the present invention also has the ability to degrade long-chain alkanes, the degradation ability can reach 69-100%, and has a good and positive application prospect in the future restoration of oil-contaminated soil.

Description

Technical field [0001] The present invention relates to the field of microorganisms, in particular to a nitrogen having degradable oil and tolerate petroleum. Background technique [0002] In biological repairs, microorganisms play an important role. During natural evolution, microorganisms pass through different catalytic mechanisms metabolic organic substances, "Storage" function enzymes as evolutionary raw materials, which can coexist with a variety of organic components, especially good at natural or synthetic organic substrates as carbon Source and energy, since the 1940s, using microbial treatment of petroleum pollution is considered a powerful strategy. [0003] During biodegradation, the addition of nutrients has an important role in activating indigenous microorganisms, promoting the petroleum hydrocarbons in microbial metabolism and degradation. However, excessive use of nutrients can result in some potential problems, such as oxygen in the over-consumption environment,...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/20C02F3/34C05G3/00C05F11/08C12R1/065
CPCC02F3/34C05F11/08C05G3/00C12N1/205C12R2001/065
Inventor王磊陈琳陈宏坤张心昱郑国砥
OwnerCHINA AGRI UNIV