Nitrogen-fixing bacterium PJ 3-5 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 3-5

A nitrogen-fixing bacteria and oil technology, applied in the field of microorganisms, can solve the problems of excessive oxygen consumption, limitation of aerobic biological oil degradation, failure, etc., and achieve the effect of positive application prospects.

Active Publication Date: 2018-08-21
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of nutrients can lead to some potential problems, such as excessive consumption of oxygen in the environment, forming an anaerobic environment, which in turn reduces the ability of indigenous microorganisms to degrade oil
Aerobic biodegradation of petroleum is often limited by combined nitrogen levels
In 2011, Mercer summarized the reported effects of biological treatment (bio-stimulation and degradative agent addition) in beach pollution treatment, and found that 10 reports were successful and 6 reports failed

Method used

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  • Nitrogen-fixing bacterium PJ 3-5 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 3-5
  • Nitrogen-fixing bacterium PJ 3-5 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 3-5
  • Nitrogen-fixing bacterium PJ 3-5 capable of degrading petroleum and tolerating petroleum and application of nitrogen-fixing bacterium PJ 3-5

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Embodiment 1

[0041] Nitrogenase activity analysis of bacterial strain PJ3-5 described in embodiment 1 of the present invention

[0042] 1. Nitrogenase determination conditions:

[0043] 1) The bacterial strain PJ3-5 of the present invention (abbreviated as PJ3-5 bacterial strain) was activated twice in LB medium at 30°C, and after transferring to liquid culture for 10 hours, the bacterial cells were washed with K.oxytoca (nitrogen-free) medium, and adjusted to OD 600 =0.2 or so, inoculate into 5mL K.oxytoca (nitrogen-free) medium anaerobic tube, 30°C, rotation speed 200rmp.

[0044] 2) When culturing for 4hr, 8hr, 12hr, 16hr, 20hr, and 24hr respectively, replace the anaerobic tube with argon, adjust the different oxygen concentrations, inject acetylene, and inject trichloroacetic acid to terminate the reaction after 0.5h of reaction, and then take 100 μL of gas was used to determine the ethylene content in the gas by gas chromatography (SQ-204 gas chromatography, FID detector, 2 m packed...

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Abstract

The invention relates to the field of microorganisms, and particularly relates to a nitrogen-fixing bacterium PJ 3-5 capable of degrading petroleum and tolerating petroleum, and the conservation number is CGMCC No.14658. The activity analysis of dinitrogenase proves that a bacterial strain has a stronger activity of dinitrogenase, and the activity of the dinitrogenase is still maintained at 75% under the condition of 5% oxygen concentration. The bacterial strain still keeps the activity of the dinitrogenase in the petroleum polluted environment with the petroleum concentration of 1%-5%. More than that, the existence of the bacterial strain further improves the growth capability of other petroleum degrading bacterial strains by 60% in a nitrogen-free culture medium with petroleum accountingfor 1%. More than that, the bacterial strain further has the capability of degrading long-chain alkane, the degrading capability is up to 69-100%, and the provided bacterial strain has an excellent positive application prospect on balancing nitrogen and degrading petroleum pollutants in the remediation of petroleum polluted soil in the future.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a nitrogen-fixing bacterium strain capable of degrading petroleum and tolerant to petroleum. Background technique [0002] In bioremediation, microorganisms play an important role. In the process of natural evolution, microorganisms metabolize organic substances through different catalytic mechanisms, and "store" functional enzymes as raw materials for evolution, which can coexist with various types of organic components, and are particularly good at using natural or synthetic organic substrates as carbon Since the 1940s, the use of microorganisms to control oil pollution has been considered a powerful strategy. [0003] During the biodegradation process, the addition of nutrients plays an important role in activating indigenous microorganisms, promoting microbial metabolism and degrading petroleum hydrocarbons in the environment. However, excessive use of nutrients can lead to some pot...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C05G3/00C05F11/08C12R1/065
CPCC02F3/34C05F11/08C05G3/00C12N1/205C12R2001/065
Inventor王磊陈琳陈宏坤张心昱郑国砥
OwnerCHINA AGRI UNIV