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A method for the determination of stable isotopes of carbon and nitrogen in samples with a high carbon-to-nitrogen ratio

A stable isotope, high carbon-nitrogen ratio technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high accuracy impact, achieve good reproducibility, improve accuracy and reproducibility, and high accuracy Effect

Active Publication Date: 2020-08-07
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the carbon-nitrogen ratio of the sample in this method has a high impact on the accuracy of the analysis results.

Method used

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  • A method for the determination of stable isotopes of carbon and nitrogen in samples with a high carbon-to-nitrogen ratio
  • A method for the determination of stable isotopes of carbon and nitrogen in samples with a high carbon-to-nitrogen ratio
  • A method for the determination of stable isotopes of carbon and nitrogen in samples with a high carbon-to-nitrogen ratio

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for determining carbon and nitrogen stable isotopes in a high carbon to nitrogen ratio sample, comprising the following steps:

[0020] a. The sample is dried at 80°C for 24 hours by soil or sediment, and ground through a 120-mesh sieve to obtain the sample to be tested.

[0021] b. Wrap the sample to be tested with a tin cup, and use IAEA-CH-6 (sucrose, δC13=-10.449‰), IAEA-CH-3 (cellulose, δC13=-24.724‰), NBS-18 ( Calcite, δC13=-5.014‰) is the standard product.

[0022] c. Wrap the sample to be tested with a tin cup, and use IAEA-N-1 (ammonium sulfate, δN15=0.4‰), IAEA-N-2 (ammonium sulfate, δN15=20.3‰) and IAEA-N0-3 in the δN15 test (potassium nitrate, δN15=25.6‰) is the standard.

[0023] d. The conditions of the elemental analyzer are: carrier gas He pressure 50kPa, injector He purge flow rate 80ml / min, oxygen pressure 15kPa, oxidation furnace 1020°C, reduction furnace 650°C, column oven 115°C, oxidation tube catalyst 25 grams of silver-plated cobalt ox...

Embodiment 2

[0029] a. Wetland soil sample δC13 detection, the sample was dried at 80°C for 24 hours, and ground through a 120-mesh sieve to obtain the sample to be tested. The carbon-nitrogen ratio of the sample was 12.85.

[0030] b. Wrap 5 mg of the sample to be tested in a tin cup, and use IAEA-CH-6 (sucrose, δC13=-10.449‰), IAEA-CH-3 (cellulose, δC13=-24.724‰), NBS-18 (calcite, δC13 =-5.014‰) is the standard product.

[0031] c. The conditions of the elemental analyzer are: carrier gas He pressure 50kPa, injector He purge flow rate 80ml / min, oxygen pressure 15kPa, oxidation furnace 1020°C, reduction furnace 650°C, column oven 115°C, oxidation tube catalyst 25 grams of silver-plated cobalt oxide and 25 grams of chromium chloride, 50 grams of granular pure copper (φ0.7mm) in the reduction tube.

[0032] d. The analysis conditions of the stable isotope mass spectrometer are: ion source vacuum 2×10 -6 mBar, ion source voltage 5KV, carrier gas He pressure 15psi, split gas He pressure 27p...

Embodiment 3

[0038] a. Wetland soil sample δN15 detection, the sample was dried at 80°C for 24 hours, and ground through a 120-mesh sieve to obtain the sample to be tested. The carbon-nitrogen ratio of the sample was 12.85.

[0039] b. Wrap 35 mg of the sample to be tested in a tin cup, and use IAEA-N-1 (ammonium sulfate, δN15 = 0.4‰), IAEA-N-2 (ammonium sulfate, δN15 = 20.3‰), IAEA-N0-3 (potassium nitrate , δN15=25.6‰) is the standard product.

[0040] c. The conditions of the elemental analyzer are: carrier gas He pressure 50kPa, injector He purge flow rate 80ml / min, oxygen pressure 15kPa, oxidation furnace 1020°C, reduction furnace 650°C, column oven 115°C, oxidation tube catalyst 25 grams of silver-plated cobalt oxide and 25 grams of chromium chloride, 50 grams of granular pure copper (φ0.7mm) in the reduction tube.

[0041] d. The analysis conditions of the stable isotope mass spectrometer are: ion source vacuum 2×10 -6 mBar, ion source voltage 5KV, carrier gas He pressure 15psi, sp...

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Abstract

The invention discloses a method for determining carbon and nitrogen stable isotopes in a high carbon-to-nitrogen ratio sample. The method comprises the following specific steps: a plant, soil or sediment sample is dried, ground through a sieve mesh, wrapped in a tin cup, and detected by elemental analyzer-stable isotope mass spectrometer. An excessive carbon dioxide signal in the high carbon-to-nitrogen ratio sample can be suppressed by the shunting effect of He on a sample gas, so that the ratio of carbon and nitrogen isotopes in the high carbon-to-nitrogen ratio sample can be rapid and accurately detected, the result is accurate, the reproducibility is good, and the method is efficient.

Description

[0001] The present invention is funded by Tianjin Application Fundamental Program (Project No. 15JCYBJC49200), Tianjin Normal University Development Fund (Project No. 52XK1505), Tianjin Normal University Municipal Key Laboratory Open Fund (Project No. 117-YF11700102). technical field [0002] The invention belongs to the technical field of stable isotope analysis, in particular to a method for detecting carbon and nitrogen isotopes in samples with a high carbon-to-nitrogen ratio. Background technique [0003] With the continuous development and improvement of carbon and nitrogen stable isotope analysis technology, its application in the environment and agriculture is becoming more and more extensive. Many scholars have used carbon and nitrogen stable isotopes to study the sources of organic matter and pollutants in oceans, lakes, wetlands, and farmland, to conduct environmental traces, and to reconstruct paleoclimate and paleovegetation through stable isotopes. [0004] With...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 尚云涛王义东陈奕霖曹佳蕊杨曦李勇李军
Owner TIANJIN NORMAL UNIVERSITY
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