Method for rapid breeding of hornwort

A hornwort, fast technology, applied in the field of hornwort cultivation, can solve problems such as lack of mature technology, and achieve the effect of promoting speed

Inactive Publication Date: 2018-11-06
四川盈乾生态环境建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of mature technology, and it is impossible to improve the cultivation speed based on large-scale engineering cultivation technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The rapid propagation method of hornwort comprises the following steps:

[0020] A, explant selection and pretreatment: select the hornwort stem section that grows vigorously, bright green in color, and can germinate axillary buds without damage by diseases and insect pests as explants, then soak with detergent, wash with sterile water and carry out pretreatment;

[0021] B, explant sterilization: process explant with alcohol and plant tissue antibacterial agent, prepare aseptic explant;

[0022] C. Bud induction: select 0.50mg / L 6-BA and 1.30mg / L IAA MS solid medium, the mass volume concentration of sucrose is 3%, the pH is adjusted to 5.8-6.0, and the solid medium is packed in a 50 ml Erlenmeyer flask , 20 ml per bottle; the solid medium was sterilized at 120°C for 20 minutes, cooled, inserted into sterilized 1-1.5 cm hornwort explants, and placed in a light incubator for static culture. Grow to 1.0-1.5cm for proliferation culture;

[0023] D. Proliferation culture:...

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PUM

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Abstract

The invention discloses a method for rapid breeding of hornwort. The breeding process is repeated, seedlings germinated, matured and sown firstly are screened each time the seedlings are bred, the selected seedlings for breeding in accordance with the above breeding method by fixing the good breeding environment are used after multi-band filtering, fast growing varieties are screened, the fast growing hornwort is used as initial seedlings for each generation, then the seedlings are continuously bred in the fixed good breeding environment, and the mating rate is commonly promoted by the speed at which the hornwort is bred, from genetic and environmental factors.

Description

technical field [0001] The invention relates to a kind of hornwort cultivation, in particular to a method for rapid propagation of hornwort. Background technique [0002] Hornwort belongs to the genus Hornwort of the family Chrysphorus. It is used as a water purification tool species and a pioneer species of vegetation restoration in lake ecological restoration projects, as bait, refuge and spawning place in the process of fish, shrimp and crab pond culture, and in the process of indoor ornamental aquaculture. as scenery material. At present, there is still a lack of mature technology, and it is impossible to improve the cultivation speed based on large-scale engineering cultivation technology. Contents of the invention [0003] The invention provides a method for rapid propagation of hornwort that solves the above-mentioned problems and improves the cultivation speed of hornwort. [0004] The present invention realizes through following technical scheme: [0005] The r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 戴晓峰姚巧玲顾继光王苏霞杨春银
Owner 四川盈乾生态环境建设有限公司
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