Chemiluminescence immunoassay kit for Seneca valley virus nonstructural protein 3ABC antibody detection

Abstract

The invention discloses a chemiluminescence immunoassay kit for Seneca valley virus nonstructural protein 3ABC antibody detection. The kit comprises a Seneca valley virus nonstructural protein 3ABC antigen coated chemiluminescence immunoassay plate, positive control serum, negative control serum, an enzyme-labeled antibody, a sample diluent, a chemiluminescence substrate solution, a luminescence enhancing agent and concentrated washing liquid, wherein the amino acid sequence of the Seneca valley virus nonstructural protein 3ABC is shown as SEQ ID NO.2. The kit provided by the invention uses nonstructural protein 3ABC antigen expressed by a prokaryotic expression system for coating a reaction plate; the antigen consumption is low; whether the Seneca valley virus exists in the pig serum or not can be efficiently detected; in addition, reaction with the swine vesicular disease viruses, hog cholera viruses and porcine circovirus type 2 cannot occur. An enzymatic chemiluminescence reactionsystem is used for judging the result; the detection sensitivity is improved. The kit provided by the invention has the advantages that the specificity is high; sensitivity and high efficiency are realized; good market prospects are realized.

Description

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AI Technical Summary

Benefits of technology

In this new discovery that describes how certain viruses cause diseases called Sickness Fever (SF) have been discovered by researchers at home or abroad. These symptoms are caused when they infect humans through contact between people who come into direct physical proximate during their interactions over long periods without being sickened from them. They also affect other animals like dogs but it's important because these harmful agents may spread quickly throughout human populations worldwide. Current methods require expensive equipment such as ELISA analysis, making testing difficult due to limited resources available.

Problems solved by technology

The problem addressed in this patented text relates to identifying and differentiate Swine Senkevices Virus Variant Causing Foot Blotch Syndrome (SFRS); specifically, finding ways to identify and separate these viruses without causing damage to humans.

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